EFFICIENCY OF USING TUBERCULOUS RECOMBINANT ALLERGEN FOR SCREENING FOR TUBERCULOUS INFECTION IN CHILDREN AND ADOLESCENTS IN STAVROPOL KRAY

The objective: to study the degree of specific sensitization according to the results of intradermal immunodiagnostic tests in children with different manifestations of tuberculosis infection under the current epidemic situation.Subjects and Methods. The degree of tuberculin sensitization was studied in 299 children from different age groups (0-14 years old) who were registered for dispensary follow-up in 2018-2019.Based on the results of a tuberculosis recombinant allergen test (TRA), the degree of specific sensitization was studied in children with tuberculin tests conversion (n = 102) and infected with Mycobacterium tuberculosis (MTB) for 2 years or more (n = 165) exposed to tuberculous infection in their families and from healthy environment, and 145 patients under 14 years old with local respiratory tuberculosis.Results. Moderate sensitization to tuberculin predominated in children with converted tuberculin test exposed to tuberculous infection in their families and those from healthy environments. According to the results of TRA test, a high degree of sensitization was observed 4.7 times more frequently in children with tuberculin test conversion exposed to tuberculosis in their families. In children from healthy environments, negative responses to TRA test were registered 2.4 times more frequently. In 73% of children infected with MTB, results of annual Mantoux tests didn’t differ much. Hyperergic reactions to TRA test in infected children exposed to tuberculous infection were 10 times more frequent than in those from healthy environments. A direct correlation was found between the high degree of sensitization to active M. tuberculosis and exposure to a tuberculosis case with a positive result of sputum test (χ2 < 0.001, p < 0.05). In the case of converted tuberculin test and infection with M. tuberculosis for 2 years or more, pronounced and hyperergic reactions to TRA test were registered with equal frequency and did not depend on the timing and duration of infection. A high risk factor and predictor of the disease development is high sensitization of the host to active M. tuberculosis which is found in patients with tuberculosis using TRA test six times more frequently versus Mantoux test (OR 5.951, 95% CI 3.548 and 9.981). The demonstrated results are important for the identification of children facing a high risk to develop active tuberculosis and preventive treatment prescription.

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Efficiency of screening for tuberculosis infection in children and adolescents in Moscow in 2019 based on the new procedure for using the intradermal test with tuberculosis recombinant allergen (ESAT-6/CFP-10)

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2021-99-1-15-25 ◽

2021 ◽

Vol 99 (1) ◽

pp. 15-25

Author(s):

L. V. Slogotskaya ◽

E. M. Bogorodskaya ◽

L. F. Shamuratova ◽

T. A. Sevostyanova

Keyword(s):

Children And Adolescents ◽

Tuberculosis Infection ◽

Intradermal Test ◽

Recombinant Allergen

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ACTUAL ISSUES OF TUBERCULOSIS IN ADOLESCENTS EXPOSED TO TUBERCULOSIS INFECTION

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2018-96-6-17-20 ◽

2018 ◽

Vol 96 (6) ◽

pp. 17-20 ◽

Cited By ~ 1

Author(s):

E. S. Ovsyankina ◽

L. V. Panova ◽

F. A. Poluektova ◽

A. Yu. Khiteva ◽

E. A. Viechelli

Keyword(s):

Health Education ◽

Life Quality ◽

Poor Performance ◽

Diagnostic Procedures ◽

Mantoux Test ◽

Skin Tests ◽

Recombinant Allergen ◽

Tuberculous Infection ◽

Hygienic Measures ◽

Close Relatives

The article characterizes respiratory tuberculosis in adolescents exposed to tuberculous infection. Exposure to tuberculosis in the family or when contacting close relatives makes the biggest contribution into development of the disease unless the patient is isolated from those exposed. Advanced and severe forms of tuberculosis with bacillary excretion are detected, compromising the life quality of adolescents. The main causes of late diagnostics are poor performance of TB services, primary medical units, low level of health education aimed at the increase of motivation to have planned medical examinations in the general population and to take relevant sanitary and hygienic measures in the sites of infection. Adolescents from the sites with bacillary excretion, and, first of all, if multiple or extensive drug resistant tuberculosis is detected, are to be considered a high priority group facing the risk to develop the disease with more frequent monitoring and deeper examination. In primary medical units, should any sings typical of tuberculosis be presented, it is sensible to add skin tests (Mantoux test and test with tuberculous recombinant allergen) to the minimum diagnostic procedures. Organizational, methodical and health education activities in the sites of infection are to be improved.

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FORMATION OF RISK GROUPS FOR TUBERCULOSIS DISEASE IN VARIOUS IMMUNOLOGICAL METHODS OF THE EXAMINATION OF CHILDREN POPULATION

Российский педиатрический журнал ◽

10.18821/1560-9561-2017-20-4-207-213 ◽

2019 ◽

Vol 20 (4) ◽

pp. 207-213 ◽

Cited By ~ 9

Author(s):

Liudmila V. Slogotskaya ◽

E. M. Bogorodskaya ◽

O. Yu. Senchikhina ◽

G. V. Nikitina ◽

D. A. Kudlay

Keyword(s):

Children And Adolescents ◽

Positive Reaction ◽

Primary Infection ◽

Risk Group ◽

Risk Groups ◽

Mantoux Test ◽

Immunological Methods ◽

High Threshold ◽

Recombinant Allergen ◽

Age Related

When performing mass tuberculin diagnostics in Moscow with coverage of more than 97% of children, the authors evaluated the effectiveness of the formation of risk groups for tuberculosis in children and adolescents with the use of immunological methods such as Mantoux test with 2 TE PPD-L and a test with an tuberculosis recombinant allergen (TRA). There was formed a risk group of cases with altered tuberculin sensitivity (group VI) in which all children underwent a skin test with ATP (Diaskintest preparation) at a dose of 0.2 μg in 0.1 ml. and in the presence of a positive reaction to this test, computed tomography was performed. The low specificity of tuberculin diagnostics in conditions of mass vaccination of children with BCG was found to lead to the fact that the frequency of positive reactions to the Mantoux test is determined primarily by postvaccinal allergy. Accumulation of cases of primary infection, hyperergia and increased reaction is less than 1% per year, and the frequency of positive reactions in the population is above 75%. It was also revealed that only 8.7-11.2% of children and adolescents with a positive reaction to the Mantoux test are examined in an anti-TB dispensary and less than 1% - are to be accounted by phthisiatricians at the risk group. In risk groups selected by the Mantoux sample, in cases with a high threshold of positive response to this test (17 mm and more), the frequency of positive responses to the TRA test is 53.3% in children and 81.4% in adolescents. With age, the proportion of primary infection among all tuberculin-positive Mantoux tests declines from 0.67% in children to 0.06% in adolescents, and the age-related positive responses for the RTA test in risk groups increase from 16.3 to 52.6% respectively. The implementation of the preventive therapy only for children with a high risk of the development of the disease (with a positive RTA test) increases the effectiveness of this therapy and excludes its unreasonable realization.

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Comparative sensitivity of the test with tuberculosis recombinant allergen, containing ESAT6-CFP10 protein, and Mantoux test with 2 TU PPD-L in newly diagnosed tuberculosis children and adolescents in Moscow

10.1101/343665 ◽

2018 ◽

Author(s):

Liudmila Slogotskaya ◽

Elena Bogorodskaya ◽

Diana Ivanova ◽

Tatiana Sevostyanova

Keyword(s):

Children And Adolescents ◽

Mass Screening ◽

Skin Testing ◽

High Sensitivity ◽

High Specificity ◽

Cost Effective ◽

Mantoux Test ◽

Recombinant Allergen ◽

Newly Diagnosed ◽

Comparative Sensitivity

AbstractBackground. A group of Russian scientists has developed Diaskintest, which comprises Mycobacterium tuberculosis-specific recombinant proteins CFP10-ESAT6, for skin testing (0.2 µg/0.1 ml).Study purpose: to evaluate the comparative sensitivity of TST with 2 TU PPD-L and a skin test with tuberculous recombinant allergen (Diaskintest) containing the ESAT6-CFP10 protein in children and adolescents with newly diagnosed respiratory tuberculosis during mass screening in the primary medical service in Moscow, 2013–2016.Materials and methods. The trial was a comprehensive retrospective group study of children and adolescents diagnosed in Moscow with respiratory tuberculosis in 2013–2016, aged 0 to 17 years inclusive. From 441 patients selected for analysis 408 patients had both tests (TST with 2 TU PPD-L and Diaskintest) performed, in 193 patients both tests were given simultaneously, of them 162 patients were BCG-vaccinated.Results. Comparative results of both tests in 408 patients with tuberculosis: at cut-off 5 mm, both tests has similar sensitivity: Diaskintest 98.3 % (95 % CI 97.0–99.6 %), TST 98.0 % (95 % CI 96.7–99.4 %), at cut-off 10 mm, the sensitivity decreases for both tests: Diaskintest 90.0 % (95 % CI 87.0–93.0 %), TST 88.7 % (95 % CI 85.6–91.9 %), but at cut-off 15 mm, the decrease in sensitivity is statistically significant: for Diaskintest 61.5 % (95 % CI 56.7–66.3 %), and for TST 46.3 % (95 % CI 41.4–51.3 %), p <0.0001.The results of simultaneous setting of tests on different hands in 193 people (including 162 BCG-vaccinated), do not differ from the results for 408 people.The correlation between the results of Diaskintest and TST was significant in all groups.Conclusion. In children and adolescents with respiratory tuberculosis, Diaskintest of 0.2 µg/ml and the Mantoux test with 2 TU PPD-L have high sensitivity (98%) at a cut-off of 5 mm; however, at cut-off 15 mm sensitivity is significantly reduced, and the decrease is more pronounced in the Mantoux test. The advantage of Diaskintest is that, unlike the Mantoux test, it has high specificity under the conditions of mass BCG vaccination. The test is cost-effective, simple to carry out, and can be used in mass screening.

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Tuberculosis Pulmonar Infantil

PEDIATRICS ◽

10.1542/peds.23.1.165a ◽

1959 ◽

Vol 23 (1) ◽

pp. 165-165

Author(s):

MARGARET H. D. SMITH

Keyword(s):

Pulmonary Tuberculosis ◽

Children And Adolescents ◽

Primary Infection ◽

Tuberculous Infection ◽

Primary Complex ◽

Hematogenous Dissemination ◽

Segmental Anatomy ◽

Congenital Tuberculosis ◽

Endobronchial Tuberculosis

This profusely and very beautifully illustrated textbook should be a welcome addition to the libraries of our Spanish-reading colleagues. Particularly good chapters on segmental anatomy of the lungs and on roentgenographic findings in the chest in tuberculous children are followed by chapters on the evolution of the primary complex; on endobronchial tuberculosis and obstructive lesions; pleurisy and pericarditis; progressive primary lesions with cavitation; hematogenous dissemination; chronic pulmonary tuberculosis of "reinfection" type in children and adolescents. Shorter chapters are devoted to such special problems as primary infection during puberty, primary infection in adults, congenital tuberculosis and the chronology of tuberculous infection in children.

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ASSESSMENT OF DANGERS FOR CHILDREN AND ADOLESCENTS WHO ARE HOUSEHOLD CONTACT OF TUBERCULOSIS INFECTION

JOURNAL OF CARDIORESPIRATORY RESEARCH ◽

10.26739/2181-0974-2020-2-14 ◽

2020 ◽

Vol 2 (1) ◽

pp. 77-80

Author(s):

Svetlana Khodjaeva ◽

◽

Dinara Adjablaeva

Keyword(s):

Prognostic Factor ◽

Children And Adolescents ◽

Social Factors ◽

Household Contact ◽

Tuberculosis Infection ◽

Tuberculous Infection ◽

Tuberculosis Patients ◽

Close Relatives

In article is evaluated danger household contact of tuberculous infection in development of the disease of children and adolescents in Samarkand state anti tuberculosis dispensary. Often in family tuberculosis was ill the mother, in most cases noted contact with the mother. In general, children and adolescents are infected as result of contact with disseminated form tuberculosis patients. The riskof the development tuberculosis of children, increased if the source of infections was earlier in prison zone. The negative social factors are considered as the prognostic factor to epidemic tension. The danger of the disease tuberculosis increases of children and adolescents from household centre tuberculosis, in which mother or two members of families (the close relatives) seek with tuberculosis. The children have a high danger of the development of the disease and particularly need for observation and undertaking preventive sanitary action

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Tuberculosis situation among the pediatric population of Saratov Region (2015-2019)

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2021-99-12-33-37 ◽

2022 ◽

Vol 99 (12) ◽

pp. 33-37

Author(s):

E. N. Aleksandrova ◽

T. I. Morozova ◽

T. Yu. Salina

Keyword(s):

Children And Adolescents ◽

Population Analysis ◽

Pediatric Population ◽

Recombinant Allergen ◽

Tuberculosis Screening ◽

Group Vi ◽

Saratov Region ◽

Number Of Children ◽

Group Iva ◽

Tuberculosis In Children

Subjects and Methods. Incidence and prevalence of tuberculosis among children and adolescents in Saratov Region were retrospectively analyzed for 2015-2019.Results. The incidence of tuberculosis in children reflects general trends in the epidemic situation over the years in Saratov Region. In this region during 10 years of monitoring, tuberculosis incidence among children decreased 4 times by 2019 and it made 2.3 per 100,000 population, and among adolescents, it decreased 1.6 times (16.3 per 100,000 population). The number of children with post-tuberculosis changes detected for the first time increased 3 times in 2019 versus 2015, and the number of adolescents increased 4.5 times not only due to transition to tuberculosis screening in children aged 8-17 years old by skin test with tuberculosis recombinant allergen but also to the examination of children by computed tomography. The incidence in children followed up as Group IVA in 2019 was 15 times higher than this rate in the overall pediatric population. Analysis of the number of children and adolescents who developed tuberculosis and followed up as Groups IV and VI in 2015-2019 showed that the number of children ill with tuberculosis in Group IV was 3 times higher than the number of children in Group VI.

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