scholarly journals A Review of Staphylococcal Cassette Chromosome mec (SCCmec) Types in Coagulase-Negative Staphylococci (CoNS) Species

2017 ◽  
Vol 24 (5) ◽  
pp. 7-18 ◽  
Author(s):  
Huda Saber ◽  
◽  
Azmiza Syawani Jasni ◽  
Tengku Zetty Maztura Tengku Jamaluddin ◽  
Rosni Ibrahim ◽  
...  
Keyword(s):  
Coagulase Negative Staphylococci ◽  
Sccmec Types ◽  
Staphylococcal Cassette Chromosome

scholarly journals Antimicrobial susceptibility pattern and SCCmec types of methicillin-resistant coagulase-negative staphylococci from subclinical bovine mastitis in Hatay, Turkey

2014 ◽  
Vol 58 (4) ◽  
pp. 563-566 ◽  
Author(s):  
Özkan Aslantaş ◽  
Mehmet Ali Yılmaz ◽  
Ebru Şebnem Yılmaz ◽  
Cemil Kurekci
Keyword(s):  
Antimicrobial Susceptibility ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
High Rate ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Antimicrobial Susceptibility Pattern ◽  
Sccmec Types ◽  
Susceptibility Patterns ◽  
Staphylococcal Cassette Chromosome

Abstract Eighty-nine isolates of coagulase-negative staphylococci (CoNS) of eight species from subclinical bovine mastitis were screened for the phenotypic and genotypic methicilline-resistance. In addition, all methicillin-resistant (MR) isolates indicating the mecA gene were examined by PCR for the antimicrobial susceptibility patterns, and staphylococcal cassette chromosome mec (SCCmec) types were also determined by multiplex PCR. A total of 21 (23.6%) CoNS isolates were found to be resistant to oxacillin in broth microdilution assay. All isolates phenotypically resistant to oxacillin did not have the mecA gene, which was only found in 14.6% (13) of the isolates. Most MR-CoNS isolates were highly resistant to erythromycin (92.3%), fusidic acid (84.6%), penicillin (76.9%), and rifampycin (61.5%), and susceptible to mupirocin (100%), tetracycline (100%), vancomycin (100%), clindamycin (92.3%), and sulfamethoxazole-trimethoprim (69.2%). In conclusion, a high rate of antimicrobial resistance among MR-CoNS isolated from food producing animals emphasises the need for periodic surveillance of their resistance.

scholarly journals Diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance

2010 ◽  
Vol 59 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Elvira Garza-González ◽  
Daniel López ◽  
Cesar Pezina ◽  
Walter Muruet ◽  
Virgilio Bocanegra-García ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Tertiary Care ◽  
Tracheal Aspirate ◽  
Type I ◽  
Care Hospital ◽  
Coagulase Negative Staphylococci ◽  
High Genetic Diversity ◽  
Type Iii ◽  
Broth Microdilution Method ◽  
Staphylococcal Cassette Chromosome

The objective of this study was to determine the distribution of staphylococcal cassette chromosome mec (SCCmec) elements in meticillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from a tertiary-care hospital in Mexico and to examine the relationship to drug resistance. Fifty selected MR-CoNS isolates collected from catheters (n=15), blood (n=15), bone (n=9), bronchial lavage (n=2) and urine (n=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion, synovial fluid, tracheal aspirate and wound secretion were examined. Susceptibility testing was performed by the broth microdilution method. SCCmec types were determined by multiplex PCR and PFGE was carried out as described previously for Staphylococcus aureus. Among the MR-CoNS strains studied, the most frequently isolated species were Staphylococcus epidermidis (n=26) and Staphylococcus haemolyticus (n=13). Staphylococcus cohnii (n=5), Staphylococcus hominis (n=3), Staphylococcus sciuri (n=1), Staphylococcus pasteuri (n=1) and the recently described species Staphylococcus pettenkoferi (n=1) were also identified. The most frequent MR-CoNS genotype identified was SCCmec type IVa in S. epidermidis isolates, which also showed a high diversity in their PFGE patterns. A clone was found that amplified both SCCmec III and V elements in five isolates examined. The single MR S. pettenkoferi isolate harboured SCCmec type IVd and the single MR S. pasteuri isolate harboured SCCmec type I. The carriage of SCCmec type III was associated with resistance or intermediate resistance to meropenem (P <0.05). These results confirm the high prevalence of S. epidermidis SCCmec IVa and the high genetic diversity among MR-CoNS strains. As far as is known, this is the first report describing the newly identified S. pettenkoferi possessing SCCmec IVd and S. pasteuri harbouring SCCmec type I. MR-CoNS harbouring SCCmec type III were found to be more resistant to meropenem.

scholarly journals Current Status of Staphylococcal Cassette Chromosome mec (SCCmec)

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 86
Author(s):  
Yuki Uehara
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Current Status ◽  
Whole Genome ◽  
Identification Methods ◽  
Sccmec Types ◽  
Staphylococcal Cassette Chromosome ◽  
Conventional Sequencing

Staphylococcal cassette chromosome mec (SCCmec) typing was established in the 2000s and has been employed as a tool for the molecular epidemiology of methicillin-resistant Staphylococcus aureus, as well as the evolution investigation of Staphylococcus species. Molecular cloning and the conventional sequencing of SCCmec have been adopted to verify the presence and structure of a novel SCCmec type, while convenient PCR-based SCCmec identification methods have been used in practical settings for many years. In addition, whole-genome sequencing has been widely used, and various SCCmec and similar structures have been recently identified in various species. The current status of the SCCmec types, SCCmec subtypes, rules for nomenclature, and multiple methods for identifying SCCmec types and subtypes were summarized in this review, according to the perspective of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements.

scholarly journals Detection of Integrons and Staphylococcal Cassette Chromosome mec Types in Clinical Methicillin-resistant Coagulase Negative Staphylococci Strains

2017 ◽  
Vol 8 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Fahimeh Hajiahmadi ◽  
Elham Salimi Ghale ◽  
Mohammad Yousef Alikhani ◽  
Alireza Mordadi ◽  
Mohammad Reza Arabestani
Keyword(s):  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Staphylococcal Cassette Chromosome

The Staphylococcal Cassette Chromosome mec (SCCmec) Analysis and Biofilm Formation of Methicillin-Resistant Staphylococcus cohnii Isolated from Clinical Samples in Tehran, Iran

2021 ◽  
Vol 16 ◽  
Author(s):  
Somaye Delfani ◽  
Faranak Rezaei ◽  
Setareh Soroush ◽  
Pegah Shakib
Keyword(s):  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Antibiotic Resistance Genes ◽  
Mobile Element ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Inducible Clindamycin Resistance ◽  
Sccmec Types

Background: Methicillin-resistant coagulase-negative staphylococci is responsible for hospital and community-acquired infections. Objective: This study aimed to investigate the antibiotic-resistance patterns, antibiotic-resistance genes, namely, ermA, ermB, ermC, blaZ, msrA, tetK, tetM, mup, and vanA, biofilm formation, and prevalence of different SCCmec types among the Staphylococcus cohniistrains isolated from clinical samples in Tehran, Iran. Methods: In this study,S. cohniiisolates were screened from the clinical samples from March 2012 to February 2013 in Tehran, Iran.Antimicrobial susceptibility test and inducible clindamycin resistance were evaluated by disc diffusion method, andresistance genes were examined using Polymerase Chain Reaction (PCR) assays. Then, biofilm formation assay was analyzed by Microtiter-plate test to detect the icaA and icaDgenes. The SCCmec and the Arginine Catabolite Mobile Element (ACME) typing were performed using the PCRmethod. Results: FromtwentyS. cohnii, all isolates were resistant to cefoxitin. 95% of the S. cohnii was defined as multidrug resistance (MDR)strains. The ermB, ermC, and vanA genes were not detected in any isolates; however, the blaZ gene had the highest frequency.95% of the S. cohnii isolates produced biofilm. Also, 4 SCCmec types, including V, IV, III+ (C2), VIII+ (AB1), were identified. Therefore, the majority of SCCmec were untypable. Based on the ACME typing, arcA and opp3 genes were positive in 13 (65%) and 1 (5%) isolates, respectively. Conclusion: Due to the high antimicrobial resistance and the spread of untypableSCCmecamong the isolates studied, the control and treatment of methicillin-resistantS. cohnii in hospitals and public health centers is a significant concern.

Characterizing a novel SCC mec with a composite structure from a clinical strain of Staphylococcus hominis , C34847

2021 ◽  
Author(s):  
Jo-Ann McClure ◽  
John M. Conly ◽  
Kunyan Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Composite Structure ◽  
Complex Structure ◽  
Clinical Strain ◽  
Coagulase Negative Staphylococci ◽  
Element Bearing ◽  
Staphylococcus Hominis ◽  
High Degree ◽  
Secondary Element ◽  
Staphylococcal Cassette Chromosome

Staphylococcal cassette chromosome mec (SCC mec ) has predominantly been described in methicillin-resistant Staphylococcus aureus . However, studies have indicated that coagulase-negative staphylococci (CoNS) carry a larger diversity of SCC elements. We characterized a composite SCC mec element carrying an uncharacterized ccr1 and type A mec gene combination, in conjunction with a secondary element bearing ccr4 , from a clinical strain of S. hominis . The element’s complex structure points to a high degree of recombination occurring in SCC mec in CoNS.

scholarly journals Occurrence of II and V Staphylococcal Cassette Chromosome mec Types among Coagulase-Negative Staphylococci from Northeastern Part of India

2018 ◽  
Vol 36 (4) ◽  
pp. 522-525
Author(s):  
Deepshikha Bhowmik ◽  
Pinki Chakraborty ◽  
Rituparna Bhowmick ◽  
Rakesh Sarkar ◽  
Shiela Chetri ◽  
...  
Keyword(s):  
Coagulase Negative Staphylococci ◽  
Northeastern Part ◽  
Staphylococcal Cassette Chromosome

scholarly journals Staphylococcal Cassette Chromosome mec (SCCmec) in coagulase negative staphylococci

2015 ◽  
Vol 17 (69) ◽  
pp. 229-233
Author(s):  
A. Martínez-Meléndez ◽  
R. Morfín-Otero ◽  
L. Villarreal-Treviño ◽  
G. González-González ◽  
J. Llaca-Díaz ◽  
...  
Keyword(s):  
Coagulase Negative Staphylococci ◽  
Staphylococcal Cassette Chromosome

scholarly journals Novel Type XII Staphylococcal Cassette ChromosomemecHarboring a New Cassette Chromosome Recombinase, CcrC2

2015 ◽  
Vol 59 (12) ◽  
pp. 7597-7601 ◽  
Author(s):  
Zhaowei Wu ◽  
Fan Li ◽  
Dongliang Liu ◽  
Huping Xue ◽  
Xin Zhao
Keyword(s):  
United States ◽  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
The United States ◽  
Gene Complex ◽  
Coagulase Negative Staphylococci ◽  
Typing Method ◽  
Content Type ◽  
Staphylococcal Cassette Chromosome ◽  
Sequence Identities

ABSTRACTExcision and integration of staphylococcal cassette chromosomemec(SCCmec) are mediated by cassette chromosome recombinases (Ccr), which play a crucial role in the worldwide spread of methicillin resistance in staphylococci. We report a novelccrgene,ccrC2, in the SCCmecof aStaphylococcus aureusisolate, BA01611, which showed 62.6% to 69.4% sequence identities to all publishedccrC1sequences. A further survey found that theccrC2gene was mainly located among coagulase-negative staphylococci (CoNS) and could be found in staphylococcal isolates from China, the United States, France, and Germany. Theccrgene complex harboring theccrC2gene was designated a type 9 complex, and the SCCmecof BA01611 was considered a novel type and was designated type XII (9C2). This novel SCCmecelement in BA01611 was flanked by a pseudo-SCC element (ΨSCCBA01611) carrying a truncatedccrA1gene. Both individual SCC elements and a composite SCC were excised from the chromosome based on detection of extrachromosomal circular intermediates. We advocate inclusion of the ccrC2gene and type 9ccrgene complex during revision of the SCCmectyping method.

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