scholarly journals Degradation of 2,4-dinitrophenol by Aspergillus niger AN 400 in batch reactors with immobilized biomass

2021 ◽  
Vol 8 (18) ◽  
pp. 623-634
Author(s):  
Renata Barros Silveira Brasil ◽  
Bárbara Chaves Aguiar Barbosa ◽  
Kelly Rodrigues ◽  
Glória Marinho

Aspergillus niger AN 400 was tested in the treatment of a synthetic water containing 2,4-dinitrophenol (2,4-DNP) by operating batch reactors with immobilized biomass. The treatment was carried out in two stages: 1) accomplished growth of fungal inoculum Aspergillus niger AN 400, and 2) the biomass was immobilized on a support at a concentration of 2 x 106 mL-1 of spores. The reaction times (RT) were 168, 48, 24 and, 8 h. The variables analyzed were COD, pH and nitrogen fractions. Decay temporal (RT168 h) of 2,4-DNP and COD adjusted to the first-order model. The higher removal efficiency 2,4 DNP was 96% and COD was 83%. There was a decrease of sequencing batch reactor SBR efficiency when RT were 8 h. The use of Aspergillus niger AN 400 may be an alternative treatment of wastewater containing nitrophenolic compounds.

Polymers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 4190
Author(s):  
Simona Popa ◽  
Andra Tamas ◽  
Vasile Simulescu ◽  
Dorin Jurcau ◽  
Sorina Boran ◽  
...  

Fatty acids esters have a wide application as bioplasticizers and biolubricants in different industries, obtained mainly in classic batch reactors, through an equilibrium complex reaction, that involves high temperatures, long reaction times, vigorously stirring, and much energy consumption. To overcome these shortcomings, we synthesized a series of fatty acid esters (soybean oil fatty acids being the acid components with various hydroxyl compounds) through novel low energy consumption technologies using a bubble column reactor, a microwave field reactor and for comparison meaning, a classic batch reactor. The obtained bioesters physicochemical properties were similar to one another, a good concordance among their rheological properties was obtained, but the energetic consumption is lower when using the bubble column or the microwave reactors instead of the classical batch reactor.


2020 ◽  
Vol 22 (2) ◽  
pp. 67-72
Author(s):  
Justyna Miłek

AbstractThe thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, thermal deactivation profiles of catalase Aspergillus niger were obtained at different temperatures in the range of 35°C to 70°C. It has been shown that the thermal deactivation of catalase Aspergillus niger follows the first-order model. The half-life time t1/2 of catalase Aspergillus niger at pH 7.0 and the temperature of 35°C and 70°C were 197 h and 1.3 h respectively. Additionally, t1/2 of catalase Aspergillus niger at the temperature of 5°C was calculated 58 months. Thermodynamic parameters the change in enthalpy ΔH*, the change in entropy ΔS* and the change Gibbs free energy ΔG* for the deactivation of catalase at different temperatures in the range of 35°C to 70°C were estimated. Catalase Aspergillus niger is predisposed to be used in biosensors by thermodynamics parameters obtained.


Author(s):  
Hongwei Sun ◽  
Chenjian Cai ◽  
Jixue Chen ◽  
Chunyu Liu ◽  
Guangjie Wang ◽  
...  

Abstract In order to investigate the effect of temperatures and operating modes on extracellular polymeric substances (EPS) contents, three sequencing batch reactors (SBRs) were operated at temperatures of 15, 25, and 35 °C (R15 °C, R25 °C, and R35 °C, respectively), with two SBRs operated under alternating anoxic/oxic conditions (RA/O and RO/A, respectively). Results showed that higher contents of tightly bound EPS (TB-EPS) and total EPS appeared in R15 °C, while loosely bound EPS (LB-EPS) dominated in R35 °C. In all three kinds of EPS (LB-EPS, TB-EPS and total EPS) assessed, protein was the main component in R15 °C and R25 °C, while polysaccharides dominated in R35 °C. Moreover, compared with RO/A, RA/O was favorable for the production of the three kinds of EPS. Furthermore, three kinds of EPS and their components were augmented during the nitrification process, while they declined during the denitrification process under all conditions except for R35 °C.


2013 ◽  
Vol 807-809 ◽  
pp. 1445-1450
Author(s):  
Jun Zhang ◽  
Hui Ping Yang ◽  
Dun Qiu Wang ◽  
Zheng Shi Zhang

A bioleaching experiment was carried out in two sequencing batch reactors (SBR) of 80 L. Acidithiobacillus thiooxidans (A. t) and Acidithiobacillus ferrooxidans (A. f) were enrichment cultured by adding sulfur powder and ferrous sulfate as substrate respectively. Sequential batch leaching ran for three periods in succession. The average removal rate of As, Cd, Cr, Cu, Ni, Pb, Zn was 90.46%, 90.28%, 90.70% and 79.70%, 81.19%, 84.52% respectively. The average loss rate of total nutrient (total account of organic matter, TN, TP and TK) is 35.36%, 33.36% and 35.54% for three runs in sequence. Comparing with A. f, the acidification time of A. t is shortened by 3.0, 6.6 and 4.9 d, with the increase of 13.5 %, 11.2% and 7.3% for the removal rate of heavy metals.


1986 ◽  
Vol 18 (6) ◽  
pp. 21-33 ◽  
Author(s):  
D. Orhon ◽  
Y. Cimşit ◽  
O. Tünay

All factors affecting substrate removal in sequencing batch reactor types of biological treatment systems should be fully explored to benefit from the many flexibilities of operation they offer. In this paper, a growth limited model is proposed for this purpose and verified by experimental observations. Interpretation of the results enabled a broader description of the process. It also indicated a number of interesting issues requiring further exploration.


2001 ◽  
Vol 44 (4) ◽  
pp. 305-312 ◽  
Author(s):  
S. M. Ratusznei ◽  
J. A.D. Rodrigues ◽  
E. F.M. Camargo ◽  
M. Zaiat ◽  
W. Borzani

The present work reports on the influence of the mechanical agitation rates on the performance of a stirred anaerobic sequencing batch reactor containing immobilized biomass on polyurethane foam, as inert support, treating synthetic domestic wastewater. The reactor was operated at 30°C and an 8-hour cycle was used to treat approximately 0.5 L of the synthetic substrate with a COD concentration of nearly 500 mg/L. The studied agitation rates ranged from no agitation to 750 rpm. The system attained non-filtered substrate removal efficiency greater than 83% when agitation was employed. A very short start-up period and good solid retention could be observed. The use of agitation increased the efficiency of the reactor and enabled reduction of the total cycle time. An empirical equation and a first-order kinetic model are proposed to analyze the influence of agitation rates on the reactor's performance.


1992 ◽  
Vol 25 (6) ◽  
pp. 239-249 ◽  
Author(s):  
J. Wanner

Sequencing batch reactors (SBR) are often used for research on nutrient removal systems. A model anaerobic-oxic SBR was compared with a compartmentalized continuous-flow system. The levels of COD, phosphorus, and nitrogen removal in both systems were comparable but the biocenoses differed significantly. The SVI values of activated sludge from the continuous reactor ranged between 100 and 200 ml/g although no significant occurrence of filamentous microorganisms was observed. The sequencing batch reactor produced activated sludge with the SVIs below 100 ml/g and with high settling velocities. Filamentous microorganisms were frequently observed in the biocenosis of the SBR. The differences in settling properties and filamentous growth in both reactors are discussed and explained.


2004 ◽  
Vol 49 (11-12) ◽  
pp. 35-40 ◽  
Author(s):  
J.H. Tay ◽  
Q.S. Liu ◽  
Y. Liu

The effect of upflow air velocity on the formation and structure of aerobic granules was studied in three column sequencing batch reactors. Upflow aeration would be the major cause of hydrodynamic shear force in the column reactor. Results showed that high upflow air velocity resulted in more compact, denser, rounder, stronger and smaller aerobic granules, while high biomass retention in the reactor was achieved. It was found that high upflow air velocity could induce granular sludge to secrete more cell polysaccharides which in turn contributed to the compact and strong structure. It appears from this study that the structure of aerobic granules could be controlled by manipulating the upflow air velocity.


2018 ◽  
Vol 196 ◽  
pp. 04009
Author(s):  
Tatiana Dormidontova ◽  
Anastasiya Filatova

The task of planning an experiment can be divided into two stages: screening experiments conducting, step-by-step experiments performed on complete factorial design or on fractional replicates. As the number of publications on the practical use of plans-experiments in construction is insufficient, the paper presents appropriate recommendations in common form. Screening experiments are usually conducted with the aim of approximate orientation among many factors and for more precise future planning. These experiments can be carried out on the minimum fractional replicates making it possible to obtain the first order model. After obtaining the model, the factors that are included in it are appropriately ranked. Using the results of ranking, researchers take into account economic considerations, leave away unimportant factors and conduct experiments with remaining factors to obtain a mathematical model of the process. In order to find out the nomenclature of these factors, screening experiments are carried out. These experiments are performed to obtain an approximate dependence between the outcome of the process and the factors. This dependence is used to determine the influence that each of the factors has on the experience result. When the crucial factors are considered, it is possible to plan the basic experiment.


2001 ◽  
Vol 43 (3) ◽  
pp. 275-282 ◽  
Author(s):  
G. Ma ◽  
N. G. Love

An experimental strategy is introduced for studying the biodegradation of wastewaters containing volatile contaminants using an alternating anoxic/microaerobic sequencing batch reactor (SBR). Benzene, toluene, and the xylene isomers (BTX) served as model volatile contaminants for this study. The reactor was configured to overcome stripping the volatile BTX compounds into the atmosphere to provide opportunities for BTX biodegradation. Oxygen-free anoxic and microaerobic (< 0.2 mg/L dissolved oxygen) conditions were established using a novel laboratory reactor configuration. ORP was successfully used to monitor different electron acceptor conditions in the SBR. Toluene and m-xylene were amenable to anoxic (denitrifying) metabolism while benzene, o-, and p-xylene were biodegradable under microaerobic conditions. The results demonstrate that establishing microaerobic conditions in full-scale bioreactors may be an appropriate way to encourage the biodegradation of aerobically biodegradable volatile contaminants. Additionally, the laboratory reactor configuration introduced in this paper may be useful in subsequent studies involving microaerobic metabolism.


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