scholarly journals GCMS, phytochemicals and antioxidant activities of in vitro callus extracts of Strobilanthes kunthiana (Nees) T. Anderson ex Benth: An endemic plant of Acanthaceae

2018 ◽  
Vol 5 (10) ◽  
pp. 359-372 ◽  
Author(s):  
R. Prabakaran ◽  
E. Kirutheka
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Dpph Method

Strobilanthes kunthiana (Neelakurinji) is an endemic and underexploited plant belongs to the Family Acanthaceae. The aim of our study was to evaluate phytochemical analysis, GC-MS analysis and antioxidant activity of in vitro callus extract of Strobilanthes kunthiana. In this present study the phytochemical analysis of various extract of S. kunthiana in vitro callus were studied. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, steroids, terpenoids and phenols in the methanolic extract comparing to the other extracts. In this study GC-MS analysis revealed the presence of 10 bioactive phytochemical compounds were identified in the methanolic extract. The prevailing compounds were 9,12-octadecadienoic acid (Z,Z) (50.32%), hexadecanoic acid, methyl ester (20.69%), 9-octadecenoic acid (Z)-,methyl ester (10.45%), heptadecanoic acid, 16-methyl-, methyl ester (5.78%), 2,6-bis(1,1-dimethylethyl)-4-methyl phenol (4.70%). 3-methyl-2-ketobutyric acid tbdms (2.79%), 2,2,3,4-tetramethyl-5-hexen-3-ol (2.41%), N-(tert-butoxycarbonyl)-2-(4-methoxyphenyl) allylamine (1.06%), cyclotrisiloxane, hexamethyl (0.94%), benzenesulfonamide (0.87%). The antioxidant property was evaluated for methanol and ethanolic extract by DPPH method. The higher percentage of inhibition (79.23 +/- 0.37) was observed in 250 μg/mL of ethanol extract followed by (90.35 +/- 0.54) methanolic extract against the standard ascorbic acid (91.25 +/- 0.33). The results show that the methanolic extract possesses more antioxidant activity than ethanol. The plant S. kunthiana may be exploited as a source of natural antioxidant and as herbal alternatives for various disorders.

scholarly journals Bioactive Variability and In Vitro and In Vivo Antioxidant Activity of Unprocessed and Processed Flour of Nine Cultivars of Australian lupin Species: A Comprehensive Substantiation

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 282 ◽  
Author(s):  
Kishor Mazumder ◽  
Afia Nabila ◽  
Asma Aktar ◽  
Asgar Farahnaky
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Acid Methyl Ester ◽  
Heat Processing ◽  
Acid Methyl ◽  
Ms Analysis

The aim of this present investigation was to analyze bioactive compounds, as well as demonstrate the antioxidant activities of nine cultivars of Australian lupin species accompanied by observing the effect of domestic heat processing on their antioxidant activities adopting in vivo and in vitro approaches. Gas chromatography mass spectroscopy (GC-MS) analysis was performed for profiling bioactive compounds present in lupin cultivars. Multiple assay techniques involving quantification of polyphenolics, flavonoids and flavonol, electron transfer (ET) based assay, hydrogen atom transfer (HAT)-based assay and in vivo assays were performed. The major compounds found were hexadecanoic acid methyl ester, 9,12-octadecadienoic acid methyl ester, methyl stearate, lupanine,13-docosenamide and 11-octadecenoic acid (Z)- methyl ester. Mandelup was found to show excellent antioxidant activity. Moreover, Jurien, Gunyidi and Barlock had strong antioxidant activity. Both positive and negative impacts of heat processing were observed on antioxidant activity. Heating and usage of excess water during processing were the key determinants of loss of antioxidants. Negligible loss of antioxidant activity was observed in most of the assays whereas inhibition of both lipid peroxidation (33.53%) and hemolysis of erythrocytes (37.75%) were increased after processing. In addition, in vitro and in vivo antioxidant assays are found to show statistically significant (* p < 0.05 and ** p < 0.01) results, which are supported by the presence of a number of antioxidant compounds in GC-MS analysis.

scholarly journals Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2392 ◽  
Author(s):  
Abdirahman Elmi ◽  
Rosella Spina ◽  
Arnaud Risler ◽  
Stéphanie Philippot ◽  
Ali Mérito ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Acacia Seyal

Water extract of Acacia seyal bark is used traditionally by the population in Djibouti for its anti-infectious activity. The evaluation of in vitro antibacterial, antioxidant activities and cytotoxicity as well as chemical characterization of Acacia seyal bark water and methanolic extracts were presented. The water extract has a toxicity against the MRC-5 cells at 256 μg/mL while the methanolic extract has a weak toxicity at the same concentration. The methanolic extract has a strong antioxidant activity with half maximal inhibitory concentration (IC50) of 150 ± 2.2 μg/mL using 1-diphenyl-2-picrylhydrazyl (DPPH) and IC50 of 27 ± 1.3 μg/mL using 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical methods. For ferric reducing/antioxidant power (FRAP) assay, the result is 45.74 ± 5.96 μg Vitamin C Equivalent (VCE)/g of dry weight (DW). The precipitation of tannins from methanol crude extract decreases the MIC from 64 µg/mL to 32 µg/mL against Staphylococcus aureus and Corynebacterium urealyticum. However, the antioxidant activity is higher before tannins precipitation than after (IC50 = 150 µg/mL for methanolic crude extract and 250 µg/mL after tannins precipitation determined by DPPH method). By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, the results showed that the condensed tannins consist of two types of catechin and gallocatechin-based oligomers. The fractionation led to the identification of three pure compounds: two flavanols catechin and epicatechin; one triterpene as lupeol; and a mixture of three steroids and one fatty acid: campesterol, stigmasterol, clionasterol, and oleamide.

scholarly journals Phytochemical analysis, cytotoxicity and antioxidant activity of cuckoo pint (Arum maculatum) leaf extract

2020 ◽  
Vol 63 (2) ◽  
pp. 119-124
Author(s):  
Hanady SA Al-Shmgani ◽  
Zahraa Hussein M Kadri ◽  
Mohammad MF Al-Halbosiy ◽  
Yaser Hassan Dewir
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Cell Line ◽  
Leaf Extract ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Enzyme Linked Immunosorbent Assay ◽  
Phytochemical Analysis ◽  
Acid Methyl ◽  
Arum Maculatum

Arum maculatum is traditionally used for the control of many diseases and illnesses such as kidney pain, liver injury, hemorrhoids. However, the detailed biomedical knowledge about this species is still lacking. This study reports on the bioactive components and the possible mechanisms underlying the antioxidant, anti-inflammatory and cytotoxic activity of A. maculatum leaf extract. Gas chromatography-mass spectrometry (GC-MS) was used for phytochemical analysis. Assay of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ) (MTT) was used to determine the cytotoxicity in the murine cell line L20B upon exposure to different extract concentrations for 24 h. Enzyme-linked immunosorbent assay (ELISA) was used to detect pro-inflammatory cytokines and tumor necrosis factor-α (TNF-α). GC-MS analysis identified the presence of important phytochemical components, e.g., 9-octadecenoic acid, methyl ester, (E), hexadecanoic acid, methyl ester, followed by benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester (17.74%), heptadecanoic acid, 16-methyl-, methyl ester and dibutyl phthalate. The results indicated a significant dose-dependent decrease in L20B cell growth at a dose of 400 μg/ml (IC50) that is associated with a significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The results suggested that the aqueous extract of A. maculatum leaves have potent antioxidant activity and cytotoxicity against L20B cell line with potential pro-inflammatory activity.

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTI-INFLAMMTORY ACTIVITY OF METHANOLIC EXTRACT OF SOLANUM NIGRUM FRUITS

2019 ◽  
Vol 1 (1) ◽  
pp. 1-5
Author(s):  
Reema Sinha
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Solanum Nigrum ◽  
Inflammatory Activity ◽  
Control Group ◽  
Root Bark ◽  
Dpph Method ◽  
Anti Inflammatory

Introduction: Solanum nigrum (Linn)  is known as Makoy and Black night shade. It is found in India and in cultivated ground. This plant has been reported for hepatoprotective, antibacterial,  antimicrobial, antiseptic, narcotic, antispasmodic, anti-inflammatory, CNS depressant, molluscidal, antiulcer, cardiac depressant, immunomodulatory and antioxidant activities. It is widely used as expectorant, anodyne, vulnerary, digestive, laxative, diuretic, cardiotonic, depurative, diaphoretic, febrifuge, swelling, wounds, ulcer, dyspepsia, ophthalmic disorder, vomiting, cardiac disorder, leprosy, skin disease, fever, splenomegaly, hemorrhoids, hoarseness, nephropathy, dropsy, gonorrhea.Leaves are use as poultice for rheumatic an gouty joints, decoction of berries and flower useful in cough, erysipelas, rat bite, bronchitis, pulmonary tuberculosis, fever, diarrhea, ophthalmopathy and hydrophobia. Root bark is useful in diseases of ear, eye, nose and hepatitis. Material and methods: For anti-inflammatory activity adult wister rats of both sexes weighing between 200-250g was used for experiment. Group-1 received 0.5% CMC suspension (control) group 2, 3 and 4 received methanolic extracts (125, 250 and 375 mg/kg) of S. nigrum respectively. Group 5 received diclofenac (reference standard 1mg/kg). The antioxidant activity of the fruit of S. nigrum was determined by using a method based on the reduction of methanolic solution of colored free radical 1, 1 di phenyl-1-2 picryl hydrazyl (DPPH). Result and discussion: Methanolic extract of Solanum nigrum Linn. fruit has shown antioxidant activity in vitro DPPH Method. The methanolic extract of S. nigrum (375 mg/kg) prevented the formation of edema induced by carrageenan and thus showed significant anti-inflammatory activity (p<0.05). Conclusion: The data collectively indicates that methanolic extract of Solanum nigrum fruits have potential anti-inflammatory and antioxidant activity.

scholarly journals GC-MS, Molecular Docking and In Vitro Studies of Methanolic Extract of Leaves of Jatropha gossypiifolia as Potent Anti-alzheimer’s

2021 ◽  
Vol 68 (1) ◽  
Author(s):  
S. Babyvanitha ◽  
Jaykar B.
Keyword(s):  
Molecular Docking ◽  
Methyl Ester ◽  
Methanolic Extract ◽  
Acetylcholinesterase Inhibitor ◽  
Research Work ◽  
Acid Methyl Ester ◽  
Phytochemical Analysis ◽  
Dodecanoic Acid ◽  
Ms Analysis ◽  
Jatropha Gossypiifolia

The aim of this study is to explore anti-alzheimer’s effect of phytocompounds identified from methanolic extract leaves of Jatropha gossypiifolia using GC-MS and Molecular docking studies. Acetylcholinesterase inhibitors improved cholinergic deficit in the brain which are beneficial in the treatment of Alzheimer’s disease. In this research work we studied the AchE antagonistic effect of phytocompounds identified from Jatropha gossypiifolia using an in silico approach. This work reported the phytochemical analysis of the methanolic extract of the leaves of Jatropha gossypiifolia. Eleven compounds were revealed through GC-MS analysis and screened using GC-MS QP2010PLUS, Shimadzu, Japan. Interpretation on mass spectrum of GC-MS was done using the database of National Institute Standard and Technology (NIST). 1,1,5-Trimethyl-1, 2-dihydro naphthalene, Methyl 10,12-pentacosadiynoate, Dodecanoic acid, methyl ester, Methyl tetradecanoate, 3,7,11,15-Tetramethyl-2-hexadecen-ol, Cyclopropanepentanoic acid 2-undecyl, methyl ester-trans, N-hexadecanoic acid, 9,12,15- Octadecatrienoic acid, Heptadecanoic acid, 16-methyl methyl ester, 7,8 Epoxylanostan 11-ol, 3-acetoxy, Cis,Cis, Cis – 7,10,13 – Hexadecatrienal compounds identified from methanolic leaf extract of Jatropha gossypiifolia using GC-MS analysis. Molecular docking of Jatropha gossypiifolia on human AchE protein was determined by using AutoDock Vina software and compared with Donepezil a known reversible acetylcholinesterase inhibitor. The interactions of Human Acetylcholinesterase-ligand conformations, including hydrogen bonds and various other bonds were analyzed using BIOVIA Discovery Studio 2016. Cis, Cis, Cis – 7,10,13-Hexadecatrienal existing phytochemicals from the leaves of Jatropha gossypiifolia had the highest fitness score of -7.6 kcal/mol comparable with donepezil having -9.2 kcal/mol and hence could be a potent antialzheimer’s drugs.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2739
Author(s):  
Liza Devita ◽  
Hanifah Nuryani Lioe ◽  
Mala Nurilmala ◽  
Maggy T. Suhartono
Keyword(s):  
Antioxidant Activity ◽  
In Silico ◽  
Antioxidant Activities ◽  
In Silico Analysis ◽  
Weight Fraction ◽  
Amino Acid Sequences ◽  
Skin Collagen ◽  
Dpph Method ◽  
Peptide Fractions

The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

scholarly journals CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SECONDARY METABOLITES FROM PULICARIA UNDULATA

2016 ◽  
Vol 8 (9) ◽  
pp. 150 ◽  
Author(s):  
Taha A. Hussien ◽  
Sayed A. El-toumy ◽  
Hossam M. Hassan ◽  
Mona H. Hetta
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Antioxidant Activities ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Hep G2 ◽  
Structure Activity ◽  
Dpph Method ◽  
Mcf 7

<p><strong>Objective:</strong><strong> </strong>To evaluate the <em>in vitro</em> cytotoxicity, antioxidant activities and structure-activity relationship of secondary metabolites isolated from <em>Pulicaria undulata</em>.</p><p><strong>Methods: </strong>The methylene chloride-methanol (1:1) extract of the air-dried aerial parts of <em>Pulicaria undulata</em> was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of the isolated compounds were determined on the basis of the extensive spectroscopic analysis, including 1D and 2D NMR and compared with the literature data. The crude extract and the isolated compounds were evaluated for <em>in vitro</em> antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method and cytotoxic assay using human breast cancer (MCF-7) and hepatoma (Hep G2) cell line.</p><p><strong>Results: </strong>Nine secondary metabolites were isolated from <em>Pulicaria undulata</em> in this study. Of which two terpenoidal compounds; 8-epi-ivalbin and 11β, 13-dihydro-4H-xanthalongin 4-<em>O</em>-β-D-glucopyranoside firstly isolated from the genus <em>pulicaria</em> and three flavonoids; eupatolitin, 6-methoxykaempferol, and patulitrin firstly isolated from <em>P. undulata</em>. 6-methoxykaempferol (IC<sub>50</sub> 2.3 µg/ml) showed the most potent antioxidant activity. The highest cytotoxic effect against MCF-7 and Hep G2 cells was obtained with eupatolitin (IC<sub>50</sub> 27.6 and 23.5 µg/ml) respectively. The structure-activity relationship was also examined and the findings presented here showed that 3, 5, 7, 4' and 3, 5, 4', 5'-hydroxy flavonoids were potent antioxidant and has cytotoxic activity.</p><p><strong>Conclusion: </strong><em>Pulicaria undulata</em> is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as antioxidant drug and in the treatment of cancer.</p>

In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum

2009 ◽  
Vol 64 (3-4) ◽  
pp. 197-200 ◽  
Author(s):  
Hari Datta Bhattarai ◽  
Babita Paudel ◽  
Hong Kum Lee ◽  
Hyuncheol Oh ◽  
Joung Han Yim
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Commercial Application ◽  
Moss Species ◽  
Antioxidant Capacities ◽  
Antioxidant Agents ◽  
Antarctic Moss

Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island (Antarctica). The identifi cation of the moss species was performed on the basis of morphological characteristics and molecular sequencing of the 18S rRNA gene. Two benzonaphthoxanthenones: ohioensins F and G, were isolated from the extract after several chromatographic procedures. The various in vitro antioxidant capacities of a methanolic extract of P. alpinum and the isolated compounds were evaluated by analyzing the scavenging capacities of free radicals of 2,2-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the total phenol assay with Folin-Ciocalteu reagent, the ferric ion (Fe3+) reducing power and the nitric oxide (NO) scavenging activity and compared to those of commercial standards for each assay. The experimental data showed that even the crude extract of P. alpinum exhibited potent antioxidant activity. The antioxidant activity was increased two- to seven-fold for the purified compounds. The antioxidant activities of both purified compounds were found to be more or less the same in all experiments. However, the obtained data showed that the Fe3+ reducing power of the purified compounds and crude methanolic extract was almost the same suggesting the presence of other stronger reducing agents in the methanolic extract which could not be isolated in the present experiment. Therefore, further work on the isolation of these stronger antioxidant agents from this moss specimen of the extreme environment is warranted. Developments of laboratory mass culture techniques are anticipated to achieve bulk production of the active constituents for commercial application.

scholarly journals QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Deepak Jain ◽  
Meenakshi Jain ◽  
Anurekha Jain
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Test Protocol ◽  
Rauwolfia Serpentina ◽  
Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

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