scholarly journals SCREENING OF C-DNA LIBRARY USING COLONY PCR

2021 ◽  
Vol 9 (08) ◽  
pp. 37-43
Author(s):  
Sakshi Narayan
Keyword(s):  
Myeloma Cells ◽  
Colony Pcr ◽  
Dna Library ◽  
The Absolute ◽  
Set Up

The principal of cdna strand was that it was combined from complete rna utilizing an oligo(dt)- containing preliminary. After oligo(dg) following the absolute cdna was enhanced by pcr utilizing two groundworks correlative to oligo(da) and oligo(dg) closures of the cdna beginning from 10 j558lμm3 myeloma cells, absolute cdna was incorporated and intensified roughly 10 5 overlay. A library containing 10 6 clones was set up from 1/6 of the enhanced cdna. Screening of the library with tests for three qualities communicated in these cells uncovered various comparing clones for each situation. The longest acquired clones contained supplements of 1. 5kb length. No arrangements starting from transporters or from rrna was found in 14 haphazardly picked clones.

Total Synthesis of (–)-Strempeliopine via Palladium-Catalyzed Decarboxylative Asymmetric Allylic Alkylation

2021 ◽  
Author(s):  
Yi An ◽  
Mengjuan Wu ◽  
Weijian Li ◽  
Yaling Li ◽  
Zhenzhen Wang ◽  
...  
Keyword(s):  
Total Synthesis ◽  
Allylic Alkylation ◽  
Asymmetric Allylic Alkylation ◽  
Enantioselective Total Synthesis ◽  
Synthetic Strategy ◽  
Palladium Catalyzed ◽  
The Absolute ◽  
Set Up

Reported herein a concise and enantioselective total synthesis of Schizozygine alkaloids (–)-strempeliopine. This synthetic strategy features a palladium-catalyzed decarboxylative asymmetric allylic alkylation of N-benzoyl lactam to set up the absolute...

Application of PPP Solution to Determine the Absolute Vertical Crustal Movements: Case Study for Northeastern Europe

2017 ◽  
Author(s):  
Kamil Kowalczyk ◽  
Janusz Bogusz
Keyword(s):  
Time Series ◽  
Vertical Movements ◽  
Crustal Movements ◽  
Northeastern Poland ◽  
The Absolute ◽  
Set Up ◽  
Changes Over Time ◽  
The Relationship ◽  
Vertical Crustal Movements

To estimate the relationship between vertical movements of the Earth’s crust, geoid temporal changes and Mean Sea Level (MSL) variations, a knowledge about the absolute (determined from satellite and space techniques) height changes over time is required. In this paper, we give an idea of determining the height changes with a use of Vertical Switching Edge Detection (VSED) algorithm. On the basis of the least squares estimation, the VSED method detects the discontinuities in time series and determines the values of jumps at the same time. We used the time series from PPP (Precise Point Positioning) solution obtained in NGL (Nevada Geodetic Laboratory) using satellite data gathered at more than 50 permanent stations located in Latvia, Lithuania and northeastern Poland. The minimum time span of data was set up to 3 years. Data were pre-analyzed by removing outliers and interpolating small gaps. The obtained results give an overview of a possibility of the proposed method to be used and the ongoing vertical movements on the area we considered.

scholarly journals On the determination of the absolute unit of resistance by alternating current methods

1912 ◽  
Vol 87 (597) ◽  
pp. 391-414 ◽  
Keyword(s):  
Lower Order ◽  
Alternating Current ◽  
National Physical Laboratory ◽  
Mutual Inductance ◽  
Electrical Circuits ◽  
Physical Laboratory ◽  
Absolute Measure ◽  
The Absolute ◽  
Set Up

1. Introductory .—Recently at the National Physical Laboratory we have constructed a standard of mutual inductance of novel type, whose value has been accurately calculated from the dimensions. This inductance has formed the basis for the determination of the unit of resistance in absolute measure by two different methods, in both of which alternating current is employed. Although there is no doubt that the accuracy attainable by these methods could be increased by greater elaboration of the apparatus used, the results already obtained seem to be of sufficient interest to warrant publication. It should be mentioned that the accuracy here aimed at was of a considerably lower order than that contemplated in the determination of the ohm by the Lorenz apparatus which is at present being carried out in the laboratory. For the experiments here described, no apparatus was specially constructed, but use was made of instruments which had already been designed and set up for the measurement of inductance and capacity. I shall first give a brief description of the standard inductance and then pass on to the methods and results. 2. Standard Mutual Inductance .—The design of the mutual inductance has already been described. The electrical circuits have the form and arrange­ment shown in section in fig. 1.

scholarly journals The Absolute Deviation Rank Diagnostic Approach to Gear Tooth Composite Fault

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Guangbin Wang ◽  
Wenhui Deng ◽  
Xiaoyang Du ◽  
Xuejun Li
Keyword(s):  
Gear Tooth ◽  
Frequency Doubling ◽  
Diagnostic Approach ◽  
Dynamics Model ◽  
Absolute Deviation ◽  
Single Fault ◽  
Simulating Analysis ◽  
Gear Fault ◽  
The Absolute ◽  
Set Up

Aiming at nonlinear and nonstationary characteristics of the different degree with single fault gear tooth broken, pitting, and composite fault gear tooth broken-pitting, a method for the diagnosis of absolute deviation of gear faults is presented. The method uses ADAMS, respectively, set-up dynamics model of single fault gear tooth broken, pitting, and composite fault gear tooth broken-pitting, to obtain the result of different degree of broken teeth, pitting the single fault and compound faults in the meshing frequency, and the amplitude frequency doubling through simulating analysis. Through the comparison with the normal state to obtain the sensitive characteristic of the fault, the absolute value deviation diagnostic approach is used to identify the fault and validate it through experiments. The results show that absolute deviation rank diagnostic approach can realize the recognition of gear single faults and compound faults with different degrees and provide quick reference to determine the degree of gear fault.

Effect of fatigue on force fluctuations in knee extensors in young adults

2010 ◽  
Vol 368 (1920) ◽  
pp. 2783-2798 ◽  
Author(s):  
Navrag B. Singh ◽  
Adamantios Arampatzis ◽  
Georg Duda ◽  
Markus O. Heller ◽  
William R. Taylor
Keyword(s):  
Young Adults ◽  
Absolute Error ◽  
Knee Extensors ◽  
Spectral Structure ◽  
Maximum Voluntary Isometric Contraction ◽  
Force Fluctuations ◽  
Frequency Domains ◽  
The Absolute ◽  
Set Up ◽  
Time And Frequency Domains

This study investigated the hypothesis that fatiguing exercises led to increased force fluctuations during submaximal isometric knee extensions and to decreased accuracy and steadiness in the time and frequency domains. Sixteen young adults (eight males, eight females) were tested, in a seated posture with 90° knee flexion, to assess their ability to reproduce target extensor torques set at 15 per cent and 20 per cent of their maximum voluntary isometric contraction, both before and after fatiguing exercises. Normalized mean (NMAE) and peak (NPAE) of the absolute error were both used to quantify accuracy, whereas normalized standard deviation of the absolute error (NSAE) was used to quantify steadiness of the torque trials in the time domain. Mean and median power frequencies (MnPF, MdPF) and normalized peak power (NPkP) were used to assess the spectral structure of the torque signals. NMAE, NSAE and NPAE all showed excellent intra- as well as intersession reliabilities (intraclass correlation values greater than 0.75 and low standard error of measurement values), demonstrating repeatability of the test set-up. NMAE, NSAE and NPAE increased significantly post-fatigue (greater than 42%), together with a shift towards higher frequency (MnPF and MdPF) components, indicating that the set-up was sensitive enough to detect the decreased force accuracy and steadiness of the musculature after fatigue. Increased force variability in both the time and frequency domains could therefore explain decreased steadiness after fatigue.

scholarly journals BIỂU HIỆN GEN SINH CƠ Ở GÀ RI LAI VÀ LƯƠNG PHƯỢNG KHI ĐƯỢC NUÔI BẰNG CÁC KHẨU PHẦN CÓ MỨC METHIONINE KHÁC NHAU

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Châu Hồ Lê Quỳnh ◽  
◽  
Trà Thân Thị Thanh ◽  
Hương Dương Thị ◽  
Dũng Đinh Văn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Breast Muscle ◽  
Muscle Weight ◽  
Myostatin Gene ◽  
Absolute Weight ◽  
Weight Calculation ◽  
The Absolute ◽  
Set Up ◽  
Expression Evaluation ◽  
Methionine Level

The aim of this study was to investigate the effects of methionine concentrations in diet on the expression of myogenic genes in Ri hybrid and Luong Phuong chickens at 12 weeks of age. A total of 240 Ri hybrid and 240 Luong Phuong one-day-old chickens of uniform body weight was divided into a 2 × 3 factorial arrangement of treatments with four replicate cages of 20 chicks per treatment. Six treatments were set up including 2 chicken types and three diets with low, adequate, high methionine concentrations compared to methionine level recommended by Evonik (2010) for colored chickens. At 12 weeks of age, 36 chickens from 2 groups were randomly chosen for the absolute and relative breast muscle weight calculation, and myogenic gene expression evaluation (myostatin, myf5, MEF2B) in breast muscle using RT-realtime PCR technique. The results showed that using the 0.08% higher of methionine level in diets than Evonik recommendation (2010) improved the absolute weight of breast muscle of the two colored chicken groups. The addition of methionine with 0.08% higher than Evonik recommendation (2010) decreased myostatin and increased MEF2B gene expression in Ri hybrid. On the other hand, using the 0.08% lower of methionine level in diets had no effect on gene expression of the three myogenic genes (myostatin, myf5 and MEF2B) comparing to using the adequate methionine diets. In the case of Luong Phuong chickens, the increasing methionine level in diets reduced myostatin gene expression, but increased myf5 and MEF2B gene expression.

The Effects of Drying Techniques on Clay-Rich Soil Texture

1974 ◽  
Vol 32 ◽  
pp. 466-467
Author(s):  
T. G. Naymik
Keyword(s):  
Critical Point ◽  
Liquid Nitrogen ◽  
Liquid Nitrogen Temperature ◽  
Drying Methods ◽  
Air Drying ◽  
Freeze Dried ◽  
Critical Point Drying ◽  
Set Up ◽  
The Relationship ◽  
Quartz Grains

Three techniques were incorporated for drying clay-rich specimens: air-drying, freeze-drying and critical point drying. In air-drying, the specimens were set out for several days to dry or were placed in an oven (80°F) for several hours. The freeze-dried specimens were frozen by immersion in liquid nitrogen or in isopentane at near liquid nitrogen temperature and then were immediately placed in the freeze-dry vacuum chamber. The critical point specimens were molded in agar immediately after sampling. When the agar had set up the dehydration series, water-alcohol-amyl acetate-CO2 was carried out. The objectives were to compare the fabric plasmas (clays and precipitates), fabricskeletons (quartz grains) and the relationship between them for each drying technique. The three drying methods are not only applicable to the study of treated soils, but can be incorporated into all SEM clay soil studies.

Evaluation of Freezing Methods by Low Temperature X-Ray Diffraction

1977 ◽  
Vol 35 ◽  
pp. 330-333
Author(s):  
T. Gulik-Krzywicki ◽  
M.J. Costello
Keyword(s):  
Biological Materials ◽  
Molecular Organization ◽  
X Ray Diffraction ◽  
Glass Capillary ◽  
X Ray ◽  
Rate Dependent ◽  
Before And After ◽  
Freeze Etching ◽  
Diffraction Patterns ◽  
Set Up

Freeze-etching electron microscopy is currently one of the best methods for studying molecular organization of biological materials. Its application, however, is still limited by our imprecise knowledge about the perturbations of the original organization which may occur during quenching and fracturing of the samples and during the replication of fractured surfaces. Although it is well known that the preservation of the molecular organization of biological materials is critically dependent on the rate of freezing of the samples, little information is presently available concerning the nature and the extent of freezing-rate dependent perturbations of the original organizations. In order to obtain this information, we have developed a method based on the comparison of x-ray diffraction patterns of samples before and after freezing, prior to fracturing and replication.Our experimental set-up is shown in Fig. 1. The sample to be quenched is placed on its holder which is then mounted on a small metal holder (O) fixed on a glass capillary (p), whose position is controlled by a micromanipulator.

Eels Under Standing Wave Conditions

1982 ◽  
Vol 40 ◽  
pp. 492-495
Author(s):  
O.L. Krivanek ◽  
J. TaftØ
Keyword(s):  
Standing Wave ◽  
Wave Conditions ◽  
Set Up ◽  
A Site ◽  
Complex Crystal

It is well known that a standing electron wavefield can be set up in a crystal such that its intensity peaks at the atomic sites or between the sites or in the case of more complex crystal, at one or another type of a site. The effect is usually referred to as channelling but this term is not entirely appropriate; by analogy with the more established particle channelling, electrons would have to be described as channelling either through the channels or through the channel walls, depending on the diffraction conditions.

Low-Loss Images in a Stem/Tem Microscope

1976 ◽  
Vol 34 ◽  
pp. 496-497 ◽  
Author(s):  
David C. Joy ◽  
Dennis M. Maher
Keyword(s):  
High Resolution ◽  
Surface Topography ◽  
Beam Direction ◽  
Low Loss ◽  
Specimen Holder ◽  
Glancing Angle ◽  
High Resolution Images ◽  
Set Up ◽  
Conventional Manner ◽  
Objective Type

High-resolution images of the surface topography of solid specimens can be obtained using the low-loss technique of Wells. If the specimen is placed inside a lens of the condenser/objective type, then it has been shown that the lens itself can be used to collect and filter the low-loss electrons. Since the probeforming lenses in TEM instruments fitted with scanning attachments are of this type, low-loss imaging should be possible.High-resolution, low-loss images have been obtained in a JEOL JEM 100B fitted with a scanning attachment and a thermal, fieldemission gun. No modifications were made to the instrument, but a wedge-shaped, specimen holder was made to fit the side-entry, goniometer stage. Thus the specimen is oriented initially at a glancing angle of about 30° to the beam direction. The instrument is set up in the conventional manner for STEM operation with all the lenses, including the projector, excited.

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