BIỂU HIỆN GEN SINH CƠ Ở GÀ RI LAI VÀ LƯƠNG PHƯỢNG KHI ĐƯỢC NUÔI BẰNG CÁC KHẨU PHẦN CÓ MỨC METHIONINE KHÁC NHAU
The aim of this study was to investigate the effects of methionine concentrations in diet on the expression of myogenic genes in Ri hybrid and Luong Phuong chickens at 12 weeks of age. A total of 240 Ri hybrid and 240 Luong Phuong one-day-old chickens of uniform body weight was divided into a 2 × 3 factorial arrangement of treatments with four replicate cages of 20 chicks per treatment. Six treatments were set up including 2 chicken types and three diets with low, adequate, high methionine concentrations compared to methionine level recommended by Evonik (2010) for colored chickens. At 12 weeks of age, 36 chickens from 2 groups were randomly chosen for the absolute and relative breast muscle weight calculation, and myogenic gene expression evaluation (myostatin, myf5, MEF2B) in breast muscle using RT-realtime PCR technique. The results showed that using the 0.08% higher of methionine level in diets than Evonik recommendation (2010) improved the absolute weight of breast muscle of the two colored chicken groups. The addition of methionine with 0.08% higher than Evonik recommendation (2010) decreased myostatin and increased MEF2B gene expression in Ri hybrid. On the other hand, using the 0.08% lower of methionine level in diets had no effect on gene expression of the three myogenic genes (myostatin, myf5 and MEF2B) comparing to using the adequate methionine diets. In the case of Luong Phuong chickens, the increasing methionine level in diets reduced myostatin gene expression, but increased myf5 and MEF2B gene expression.