scholarly journals Astaxanthin Recovery from Shrimp Residue by Solvent Ethanol Extraction Using Choline Chloride:Glycerol Deep Eutectic Solvent as Adjuvant

2021 ◽  
Author(s):  
Cleidijane dos Santos ◽  
Carlos Padilha ◽  
Karla Damasceno ◽  
Pedro Leite ◽  
Ana de Araújo ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Deep Eutectic Solvent ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Total Carotenoid ◽  
Total Carotenoids ◽  
Clear Trend ◽  
Ethanol Extraction ◽  
Gram Negative ◽  
Ultrasound Assisted

The present study investigated the use of choline chloride:glycerol deep eutectic solvent as an adjuvant for tuning the ethanol extraction of astaxanthin from Litopenaeus vannamei processing residues. The adjuvant concentration did not show a clear trend on the total carotenoid, however, the addition of adjuvant proved to be advantageous in the ultrasound-assisted experiments. In order to establish a balance between total carotenoid and productivity, an operational condition with ultrasound, 5% (m/v) choline chloride:glycerol and 10 min of incubation was selected, which provided 737.69 μg g-1 total carotenoids and 32.71 μg g-1 astaxanthin. When compared to the astaxanthin standard, the shrimp residue extract obtained greater antioxidant activity in free radical scavenging tests. In addition, the shrimp residue extract dramatically reduced the dosage of norfloxacin (up to 87.5%) and gentamicin (up to 75.0%) antibiotics in tests to inhibit the growth of gram-negative bacteria.

scholarly journals Antibacterial, Antioxidant and Cytotoxic Properties of Crinum asiaticum Bulb Extract

2012 ◽  
Vol 28 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Md Atiar Rahman ◽  
Rumana Sharmin ◽  
Md Nazim Uddin ◽  
Md Sohel Rana ◽  
Nazim Uddin Ahmed
Keyword(s):  
Radical Scavenging ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Scavenging Effect ◽  
Ic50 Value ◽  
Radical Scavenging Effect ◽  
Dpph Scavenging ◽  
Crinum Asiaticum

Antibacterial effect of Crinum asiaticum bulb extract (1mg/disc) was tested on four Gram- positive and six Gram-negative bacteria by disc diffusion method using kanamycin (30 ìg/disc) as standard antibiotic disc. The bulb extract (250-1000mg/disc) showed significant zone of inhibition against all Gram-positive and Gram-negative bacteria ranging from 12-14 mm in diameter. Antioxidant potential of the same extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The extract showed remarkable free radical scavenging effect (95.96%) providing the IC50 value of 5.62 for the bulb extract and 5.46 for ascorbic acid (standard antioxidant) at the concentration of 1000 ìg/ml. The bulb extract was found to be (LC50 value 94.06 ?g/ml) in Brine-Shrimp lethality test. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11801 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 1-5

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

scholarly journals Analysis of Phytochemical Constituents and Biological Activity of Taxus Wallichiana Zucc. Dolakha District of Nepal

2018 ◽  
Vol 6 (2) ◽  
pp. 110-114
Author(s):  
Bimala Subba
Keyword(s):  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Pharmacologically Active ◽  
Taxus Wallichiana

Phytochemical and biological activities of methanolic extract of Taxus wallichiana Zucc. (Leaf, stem) were carried out. The brine shrimp bioassay showed T. wallichiana is pharmacologically active. The antibacterial potential was studied against one gram positive bacteria (Staphylococcus aureus) and one gram negative bacteria (Escherichia Coli) using Agar Well Diffusion Method. Stem of T. wallichiana showed significant zone of inhibition against gram positive bacteria while the leaf of T. wallichiana did not show significant zone of inhibition against both gram positive and gram negative bacteria. Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and FRAP assay. Both assay showed that T. wallichiana leaves has high antioxidant activities.Int. J. Appl. Sci. Biotechnol. Vol 6(2): 110-114

scholarly journals Analysis of Phytoconstituents and Biological Activities on The Selected Medicinal Plants of Dolakha and Sindhualchowk District of Nepal

2018 ◽  
Vol 22 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Bimala Subba ◽  
Susila Thapa
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Antioxidant Power ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Pharmacologically Active

Phytochemical and biological activities of methanolic extract of seven plants viz. Scindapsus officinalis Schott. , Lepisorus loriformis, Nicotiana tabacum L., Clematis buchananiana DC., Astilbe rivularis D. Don, Potentilla fulgens Wall. ex Hook and Taxus wallichiana Zucc. were carried out. The brine shrimp bioassay showed that N. tabacum, A. rivularis, and P. fulgens were pharmacologically active. The antibacterial potential was studied against Staphylococcus aureus (gram positive bacteria) and Escherichia coli (gram negative bacteria) using Agar Well Diffusion Method. Roots of P. fulgens showed inhibition against gram positive bacteria while the rhizome of A. rivularis showed inhibition against both gram positive and gram negative bacteria. Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power FRAP assay. Both assays showed that P. fulgens has high antioxidant activities with (IC50 =15.57 ± 3.6) lower than standard ascorbic acid.  Journal of Institute of Science and TechnologyVolume 22, Issue 2, January 2018, Page: 140-147

scholarly journals Optimizing Ultrasound-Assisted Deep Eutectic Solvent Extraction of Bioactive Compounds from Chinese Wild Rice

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2718 ◽  
Author(s):  
Jia Zeng ◽  
Yuqing Dou ◽  
Ning Yan ◽  
Na Li ◽  
Huaibao Zhang ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Wild Rice ◽  
Choline Chloride ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Deep Eutectic Solvent ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Green Solvents ◽  
Ultrasound Assisted

In this study, deep eutectic solvents (DESs) were used for the ultrasound-assisted extraction (UAE) of valuable bioactive compounds from Chinese wild rice (Zizania spp.). To this end, 7 different choline chloride (CC)-based DESs were tested as green extraction solvents. Choline chloride/1,4-butanediol (DES-2) exhibited the best extraction efficiency in terms of parameters such as the total flavonoid content (TFC), total phenolic content (TPC), and free radical scavenging capacity (DPPH● and ABTS●+). Subsequently, the UAE procedure using 76.6% DES-2 was also optimized: An extraction temperature of 51.2 °C and a solid–liquid ratio of 37.0 mg/mL were considered optimal by a Box–Behnken experiment. The optimized extraction procedure proved efficient for the extraction of 9 phenolic and 3 flavonoid compounds from Chinese wild rice as determined by quantification based on ultra-performance liquid chromatography–triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS). This work, thus, demonstrates the possibility of customizing green solvents that offer greater extraction capacity than that of organic solvents.

scholarly journals Phytoconstituents and biological analysis of Acorus calamus rhizome of Sindhupalchowk District, Nepal

BIBECHANA ◽  
2020 ◽  
Vol 17 ◽  
pp. 104-109
Author(s):  
Shanta Pokhrel ◽  
Kalpana Chaulagain
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Acorus Calamus ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative

Phytochemical and biological activities of methanolic and hexane extract of Acorus calamus rhizome were carried out. Phytochemical analysis showed the presence of flavonoid, glycoside, saponin, resin and carbohydrates which are responsible for the antibacterial activities. The antibacterial potential was studied against Staphylococcus aureus (gram positive bacteria) and Escherichia coli, Salmonella typhi (gram negative bacteria) using Agar Well Diffusion Method. The activity was shown by both methanolic and hexane extract of Acorus calamus rhizome against both Gram positive and Gram negative bacteria. Antioxidant activity of methanolic extract was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity showed potent antioxidant activities with IC50 value 3.74 μg/mL slightly higher than standard ascorbic acid (IC50 = 3.56 μg/mL). BIBECHANA 17 (2020) 104-109

In Vitro Antimicrobial and Antioxidant Activity of Commercial Rosemary Extract Formulations

2009 ◽  
Vol 72 (8) ◽  
pp. 1744-1752 ◽  
Author(s):  
ANJA KLANČNIK ◽  
BERNARDA GUZEJ ◽  
MAJDA HADOLIN KOLAR ◽  
HELENA ABRAMOVIČ ◽  
SONJA SMOLE MOŽINA
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Water Soluble ◽  
Total Phenolic ◽  
Gram Negative ◽  
Antimicrobial And Antioxidant Activity

Phenolic plant extracts are sources of natural bioactive compounds, which can inhibit the rate of food spoilage. MIC and MBC concentrations of four oil- or water-soluble rosemary (Rosmarinus officinalis) extracts against gram-positive (Bacillus and Staphylococcus) and gram-negative (Campylobacter and Salmonella) bacteria were determined by using disk diffusion, agar dilution, and broth microdilution methods, as well as bacterial survival kinetics in a macrodilution test. To describe the antioxidant properties of the extracts, the reducing power, free radical scavenging effectiveness, and β-carotene bleaching test were used. The antimicrobial and antioxidant activity depended on the concentration and chemical nature of the phenolic compounds in the extracts. Gram-positive bacteria were more sensitive than were gram-negative bacteria, especially for oil-soluble extracts with carnosic acid as the major phenolic compound. A microdilution method based on ATP measurement was found to be a useful, rapid technique for determining antibacterial efficiency, and its results correlated well with MICs from survival curve measurement. Reducing power and free radical scavenging effectiveness was higher in water-soluble formulations, according to their higher total phenolic content, but in an aqueous emulsion system of linoleic acid, they exhibited lower antioxidant activity. This correlated well with the higher efficiency of antimicrobial activity of oil-soluble formulations, despite the lower total phenolic content of these extracts.

scholarly journals Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity

2020 ◽  
Vol 11 (4) ◽  
pp. 76
Author(s):  
Erum Dilshad ◽  
Mehmoona Bibi ◽  
Nadeem Ahmed Sheikh ◽  
Khairul Fikri Tamrin ◽  
Qaisar Mansoor ◽  
...  
Keyword(s):  
Citric Acid ◽  
Maleic Acid ◽  
Brine Shrimp ◽  
Radical Scavenging ◽  
Sem Analysis ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Silver Particles ◽  
X Ray ◽  
Average Size

An accumulating body of evidence reports the synthesis and biomedical applications of silver nanoparticles. However, the studies regarding the use of maleic acid and citric acid in the synthesis of nano-sized silver particles (AgNPs) and micro-sized silver particles (AgMPs) as well as their antibacterial, antifungal, and anticancer activities have not been reported. In the current study, we synthesized AgNPs and AgMPs using maleic acid and citric acid as capping agents and have characterized them by UV-Vis, energy-dispersive X-Ray spectroscopy (EDS), X-Ray diffraction (XRD), and scanning electron microscope (SEM) analysis. The capped silver particles were examined for their antimicrobial activity and cytotoxicity against bacteria, fungi, and brine shrimp. Additionally, the anticancer activity of these particles was tested against human breast and liver cancer cell lines. The free radical scavenging activity of capped silver particles was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. SEM analysis revealed a round plate-like morphology of maleic acid capped particles with an average size of 39 ± 4 nm, whereas citric acid capped particles display flower-shaped morphology with rough surfaces and an average size of 250 ± 5 nm. The uncapped AgMPs were hexagonal with 500 ± 4 nm size. EDS and XRD analysis confirmed the presence of Ag and face-centered cubic crystalline nature, respectively. Functionally, capped silver particles exhibited antibacterial activity against Gram-positive (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) and Gram-negative bacteria (Salmonella setubal, Enterobacter aerogenes, and Agrobacterium tumefaciens). The bactericidal activity was more active against Gram-negative bacteria with minimum inhibitory concentration (MIC) as low as 5 ppm as compared to 25 ppm for Gram-positive. Similarly, the silver particles demonstrated antifungal activity by inhibiting the growth of five fungal strains (Mucor species, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, and Fusarium solani) up to 50% at the concentration of 500 ppm. Additionally, these particles showed substantial toxicity against brine shrimp and also significantly inhibited the proliferation of breast cancer (MCF7) and liver cancer (HePG2) cell lines (IC50 8.9–18.56 µM). Uncapped AgMPs were less effective, inhibiting only the proliferation of MCF7 cells with IC50 46.54 µM. Besides cytotoxicity, these particles acted as potential antioxidants, showing free radical scavenging up to 74.4% in a concentration-dependent manner. Taken together, our results showed that the modifiers affect the shape and size of silver particles and may, in part, contribute to the antimicrobial and antioxidant activity of silver particles. However, the contribution of maleic acid and citric acid in enhancing the antimicrobial, anticancer, and antioxidant potential independent of silver nano and microparticles needs to be studied further. In vivo experiments may determine the therapeutic effectiveness of silver particles capped with these modifiers.

scholarly journals Japanese Quince (Chaenomeles japonica) as a Potential Source of Phenols: Optimization of the Extraction Parameters and Assessment of Antiradical and Antimicrobial Activities

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1132
Author(s):  
Ieva Urbanavičiūtė ◽  
Mindaugas Liaudanskas ◽  
Česlovas Bobinas ◽  
Antanas Šarkinas ◽  
Aistė Rezgienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Japanese Quince

The value of fruits is determined by the quantity and variety of biologically active compounds they contain, and their benefits on human health. This work presents the first study of the biochemical composition and antibacterial activity of the new Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ fruits. The total phenolic content (TPC) was determined using the Folin–Ciocalteu method and each compound was identified by HPLC High Performance Liquid Chromatography. The antimicrobial activity against three Gram-positive and three Gram-negative bacteria, and one yeast strain, was evaluated by the agar well diffusion method using three different concentrations. The free radical scavenging activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) methods and ranged from 99.1 to 115.9 μmolTE/100 g, and from 372 to 682 μmolTE/100 g, respectively. TPC ranged from 3906 to 4550 mgGAE/100 g, and five compounds, isoquercitrin, rutin, (+)-catechin, (–)-epicatechin, and chlorogenic acid were identified. All JQ extracts possessed antimicrobial activity against Gram-positive and Gram-negative bacteria, and Enterococcus faecalis (ATCC 29212) was the most sensitive strain. These results indicate that JQ fruits are a significant source of bio-compounds, which can enrich the diet with strong antioxidants, and they are very promising as a substitute for chemical preservatives in the food and cosmetic industry.

scholarly journals Phytochemical characterization, and antioxidant and antimicrobial activities of essential oil from leaves of the common sage Salvia officinalis L. from Abha, Saudi Arabia

2020 ◽  
Vol 14 (6) ◽  
pp. 261-270 ◽  
Author(s):  
Atef El Jery ◽  
Mudassir Hasan ◽  
Md Mamoon Rashid ◽  
Mohammed Khaloofah Al Mesfer ◽  
Mohd Danish ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Salvia Officinalis ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
The Common

AbstractBackgroundThe composition and activities of essential oil of common sage from Saudi Arabia have not yet been reported.ObjectivesTo analyze the composition and antibacterial and antioxidant activities of essential oil from leaves of the common sage Salvia officinalis L. from Abha, Saudi Arabia.MethodsEssential oil was extracted from the leaves of S. officinalis by hydrodistillation, and its composition was analyzed using gas chromatography and mass spectrometry. Phenolics and flavonoids were determined using gallic acid and quercetin standards. Antioxidant activity was determined using a 2,2-diphenyl-1-picrylhydrazyl radical scavenging method. Activity against various gram-positive and gram-negative bacteria was determined by disk diffusion and microdilution.ResultsThe yield of essential oil was 3.24 ± 0.55% (w/dry weight). Major compounds identified were camphor (20.3%), 1,8-cineole (15.0%), α-thujone (14.9%), viridiflorol (9.9%), carvone (6.2%), and β-thujone (5.7%). Phenolic content was 134.3 ± 17.61 μg/mL and flavonoid content was 119.5 ± 18.75 μg/mL. Antioxidant IC50 was 970 ± 5.5 μg/mL. The highest gram-positive antibacterial activity was for Bacillus subtilis and the highest gram-negative activity was for Escherichia coli. Minimum inhibitory concentrations ranged from 62.2 ± 3.9 to 1398.1 ± 50.7 μg/mL for gram-positive bacteria and from 323.4 ± 69.5 to 968.4 ± 120.6 μg/mL for gram-negative bacteria. Minimum bactericidal concentrations ranged from 120.3 ± 7.6 to 1387.4 ± 161.8 μg/mL for gram-positive bacteria and from 386 ± 8.3 to 1225.2 ± 100.9 μg/mL for gram-negative bacteria.ConclusionsEssential oil of S. officinalis L. from Abha, Saudi Arabia, showed compositional, antioxidant, and antibacterial properties generally consistent with essential oil of S. officinalis L. from other locations as reported in the literature.

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