scholarly journals Synergistic effect of certain natural permeabilizers with antimicrobial agents on outer membrane of some multidrug Gram- negative pathogenic bacteria

2015 ◽  
Vol 32 (part 1) ◽  
pp. 107-121 ◽  
Author(s):  
Hala Farrag ◽  
Mona Shehata ◽  
Nagwa Abdallah ◽  
Ebthag Awad
Keyword(s):  
Synergistic Effect ◽  
Outer Membrane ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Gram Negative

An outlook for food sterilization technology: targeting the outer membrane of foodborne gram-negative pathogenic bacteria

2021 ◽  
Vol 42 ◽  
pp. 15-22
Author(s):  
Zhaohuan Zhang ◽  
Zhenhua Huang ◽  
Jinrong Tong ◽  
Qian Wu ◽  
Yingjie Pan ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Pathogenic Bacteria ◽  
Gram Negative ◽  
Food Sterilization

Synergistic Activity of Phenolic Compounds of Some Plants Against Bacteria

2021 ◽  
Vol 8 (6) ◽  
pp. 102-110
Author(s):  
Roaa M. H. Shoker
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Synergistic Effect ◽  
Pathogenic Bacteria ◽  
Total Concentration ◽  
Sesamum Indicum ◽  
Gram Negative Bacteria ◽  
Synergistic Activity ◽  
Gram Negative ◽  
Phenolic Extracts

Medicinal plants produce great groups of secondary metabolites which are essential for medicine purpose, one of them phenolic compounds, antimicrobial activity of phenolic compounds which derivative from plants has been examined for several years. The phenolic extracts of Sesamum indicum and Pimpinella anisum seeds have antibacterial action against Gram positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), (Acinetobacter baumannii), and (Pseudomonas aeruginosa) (Proteus mirabilis). The current findings show that the synergistic impact of phenolic extracts from S. indicum and P. anisum is active against a variety of pathogenic bacteria, and that the synergistic effect for two plants is more antibacterial than phenolic extracts from one plant.  The results indicated Gram- negative (P. aeruginosa) more effected by plants, than Gram-negative (S. aureus) which have the lower effects. The results of HPLC indicated Sesame (S. indicum) have total concentration of phenolic compounds was (1313.7 µg/ml) higher than total concentration of phenolic compounds of Anise (P. anisum) (220.991 µg/ml), and have varied types of phenolic compounds were Pyrogallol, Gallic acid, Rutin, Kaempferol, Cinnamaldehyde, Qurctin, Eugenol, Lignan with different concentration. From this study may be conclusion Synergistic effect for two plants have more antibacterial than phenolic extracts of one plant, and Sesame (S. indicum) have higher antimicrobial activity than Anise (P. anisum).

scholarly journals Ciprofloxacin-Resistant Gram-Negative Bacilli in the Fecal Microflora of Children

2006 ◽  
Vol 50 (10) ◽  
pp. 3325-3329 ◽  
Author(s):  
Xuan Qin ◽  
Yasmin Razia ◽  
James R. Johnson ◽  
Jennifer R. Stapp ◽  
Daniel R. Boster ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Enterobacter Aerogenes ◽  
Fecal Excretion ◽  
Achromobacter Xylosoxidans ◽  
Resistant Bacteria ◽  
Phylogenetic Groups ◽  
Gram Negative ◽  
E Coli ◽  
Public Health Challenges

ABSTRACT The extent to which antibiotic-resistant bacteria are excreted by humans who have not been exposed to antibiotics is not known. Children, who rarely receive fluoroquinolones, provide opportunities to assess the frequency of fecal excretion by fluoroquinolone-naïve hosts of fluoroquinolone-resistant gram-negative bacilli. Fresh nondiarrheal stools from children were processed by screening them on agar containing ciprofloxacin to recover ciprofloxacin-resistant gram-negative bacilli. Resistant isolates were identified, and ciprofloxacin MICs were determined. Resistant Escherichia coli isolates were also analyzed for urovirulence-associated loci. Thirteen (2.9%) of 455 stools yielded ciprofloxacin-resistant E. coli (seven children), Stenotrophomonas maltophilia (four children), and Achromobacter xylosoxidans and Enterobacter aerogenes (one child each). Neither the subjects themselves nor members of their households used fluoroquinolones in the 4 weeks preceding collection. Six of the seven resistant E. coli isolates belonged to phylogenetic groups B2 and D, in which extraintestinal pathogenic E. coli bacteria are frequently found. All resistant E. coli isolates contained at least three putative E. coli virulence loci. Most ciprofloxacin-resistant bacteria were resistant to additional antibiotics. Potentially pathogenic bacteria that are resistant to therapeutically important antimicrobial agents are excreted by some humans, despite these persons' lack of exposure to the particular drugs. The sources of these resistant organisms are unknown. This underrecognized reservoir of drug-resistant potential pathogens poses public health challenges.

Development of benzimidazole-based derivatives as antimicrobial agents and their synergistic effect with colistin against gram-negative bacteria

2020 ◽  
Vol 186 ◽  
pp. 111850 ◽  
Author(s):  
Eman M.E. Dokla ◽  
Nader S. Abutaleb ◽  
Sandra N. Milik ◽  
Daoyi Li ◽  
Karim El-Baz ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Antimicrobial Agents ◽  
Gram Negative Bacteria ◽  
Gram Negative

scholarly journals Carriage of multi-drug resistant Gram-negative pathogenic bacteria by the house fly Musca domestica

2017 ◽  
Vol 26 (1) ◽  
pp. 91-99
Author(s):  
Tangin Akter ◽  
Sangita Ahmed ◽  
Badhan Rani Das
Keyword(s):  
Body Surface ◽  
Pathogenic Bacteria ◽  
Alimentary Tract ◽  
Antimicrobial Agents ◽  
House Fly ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
House Flies ◽  
E Coli ◽  
External Body

Fifteen house flies were used to isolate bacteria from external body surface and alimentary tract. A total of 50 isolates were obtained from the house flies, of which 25 (50%) were collected from the external body surface and 25 (50%) from alimentary gut. Fifteen isolates (30%) were obtained from Shamsunnahar Hall (SN) dining room, 22 (44%) from Dhaka Medical College Hospital (DMCH) and 13 (26%) from Rokeya Hall (RH) canteen. Six Gram-negative bacteria were isolated from the house flies namely, Escherichia coli (36%), Shigella spp. (22%), Salmonella spp. (18%), Pseudomonas spp. (10%), Klebsiella spp. (8%) and Enterobacter spp. (6%). E. coli was the highest in number in all three study areas which was 33% in SN Hall dining, 36% in DMCH, and 39% in RH canteen. E. coli was present in 32 and 40% of external body surface and gut samples, respectively. Bacterial susceptibility to antimicrobial agents showed that E. coli isolates were highly resistant (66-77.7%) to ampicillin, ciprofloxacilin and penicillin antibiotics. Salmonella isolates were sensitive to chloramphenicol but it was (55.5%) resistant to ampicillin, penicillin, tetracycline, gentamycin and imipenem antibiotics. In case of Shigella and Pseudomonas, 72.72 and 80% isolates were resistant to tetracycline and chloramphenicol, respectively. Among the Enterobacter spp. 66.66% were resistant to chloramphenicol, imipenem, vancomycin and tetracycline, while Klebsiella showed 100% resistant pattern to tetracycline in the study. It was observed that house flies carry several multidrug resistant Gram-negative bacteria in their body surface and alimentary tract and played a role in the transmission of serious diseases to human. Dhaka Univ. J. Biol. Sci. 26(1): 91-99, 2017 (January)

scholarly journals Carbapenem Resistance in Gram-negative Bacteria in South-western Nigeria: The Role of Extended-spectrum β-lactamase CTX-M-15

2018 ◽  
pp. 344-349
Author(s):  
Do Ogbolu ◽  
Ma Webber
Keyword(s):  
Outer Membrane ◽  
Antimicrobial Agents ◽  
Random Amplified Polymorphic Dna ◽  
Outer Membrane Proteins ◽  
Carbapenem Resistance ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Carbapenem Resistant ◽  
Extended Spectrum

Objective: To determine the role of extended-spectrum β-lactamases in carbapenem-resistant Gram-negative bacteria from south-western Nigeria. Methods: Twenty-seven carbapenem-resistant isolates that were found to be non-carbapenemase producers (15 Escherichia coli, 9 Klebsiella pneumoniae and 3 Pseudomonas aeruginosa) were further studied. These isolates were subjected to analysis including phenotypic and genotypic detection of various β-lactamases, efflux activity, outer membrane protein, plasmids replicon typing, detection of transferable genes and resistances and typing using random amplified polymorphic DNA tests. Results: No isolates demonstrated de-repression of efflux, but all showed either complete loss or reduced production of outer membrane proteins. Transconjugants from these strains contained various genes including plasmid-mediated quinolone resistance and extended-spectrum beta-lactamases. All the transconjugants carried the blaCTX-M-15 gene. The transconjugants had varying minimum inhibitory concentrations of carbapenems ranging from 0.03 μg/ml to 8 μg/ml. Varying resistances to other antimicrobial agents were also transferred with the plasmids. The donor isolates were not clonally related by molecular typing. Conclusion: Resistance to carbapenem antibiotics in this sample was not mediated only by carbapenemases but also by production of extended-spectrum β-lactamases (largely CTX-M-15), accompanied by protein loss. This was an important mechanism underpinning carbapenem resistance in these clinical isolates of various species.

Bacteriocins active against plant pathogenic bacteria

2012 ◽  
Vol 40 (6) ◽  
pp. 1498-1502 ◽  
Author(s):  
Rhys Grinter ◽  
Joel Milner ◽  
Daniel Walker
Keyword(s):  
Recent Work ◽  
Pathogenic Bacteria ◽  
Plant Disease ◽  
Antimicrobial Agents ◽  
Genetic Resistance ◽  
Diverse Range ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Identification And Characterization

Gram-negative phytopathogens cause significant losses in a diverse range of economically important crop plants. The effectiveness of traditional countermeasures, such as the breeding and introduction of resistant cultivars, is often limited by the dearth of available sources of genetic resistance. An alternative strategy to reduce loss to specific bacterial phytopathogens is to use narrow-spectrum protein antibiotics such as colicin-like bacteriocins as biocontrol agents. A number of colicin-like bacteriocins active against phytopathogenic bacteria have been described previously as have strategies for their application to biocontrol. In the present paper, we discuss these strategies and our own recent work on the identification and characterization of candidate bacteriocins and how these potent and selective antimicrobial agents can be effectively applied to the control of economically important plant disease.

scholarly journals Prevalence of Pathogenic Bacteria Isolated from Surgical Site and Wound Infection among Patients Admitted in some selected Hospitals in Sokoto Metropolis, Nigeria

2014 ◽  
Vol 3 (3) ◽  
pp. 89-103
Author(s):  
UK Muhammad ◽  
TM Adamu ◽  
Z Binji ◽  
MA Isa
Keyword(s):  
Wound Infection ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Gram Negative Bacteria ◽  
Citrobacter Freundii ◽  
Gram Positive ◽  
Gram Negative ◽  
Predominant Pathogen ◽  
Women And Children ◽  
The Mean

Surgical and open wounds are commonly encountered in clinical practice. This study was aim to determine the prevalence of pathogenic bacteria in surgical and open wound infection among patients admitted in some selected hospitals in Sokoto metropolis. A total of one hundred and fifty one (151) isolates were obtained from two hundred (200) surgical site and wound samples collected from patients in this study. The result showed that Usmanu Danfodiyo Teaching Hospital Sokoto (UDUTH) had the highest number of clinical isolates with 64 gram positive and gram negative bacteria followed by Specialist Hospital Sokoto (S.H.S) with 57 gram positive and gram negative bacteria and then Maryam Abacha Women and Children Hospital (MAWCH) with 30 gram positive and gram negative bacteria. Gram positive cocci 108 (71.5%) were more predominant pathogen isolated in the hospitals than gram negative bacilli 43 (28.5%). Staphylococcus aureus had the highest number of occurrence with 54(35.76%) followed by Coagulate negative Staphylococci with 47(31.1%) while Citrobacter freundii had the lowest number of occurrence with 2(1.32%) isolates. Also, the susceptibility of the isolates to antimicrobial agents were carried out using Amoxacillin, Ampicillin, Erythromycin, Chloramphenicol, Ampiclox, Ciprofloxacin, Gentamycin, Tetracycline, Pefloxacin and Cotrimoxazole. The mean zone of inhibition recorded against Staphlococcus aureus by using Amoxacillin antibiotic is 2.20mm while with Citrobacter freundii is 1.00. DOI: http://dx.doi.org/10.3126/ije.v3i3.11066 International Journal of Environment Vol.3(3) 2014: 89-103

scholarly journals Gene Transfer Potential of Outer Membrane Vesicles of Gram-Negative Bacteria

2021 ◽  
Vol 22 (11) ◽  
pp. 5985
Author(s):  
Federica Dell’Annunziata ◽  
Veronica Folliero ◽  
Rosa Giugliano ◽  
Anna De Filippis ◽  
Cristina Santarcangelo ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Outer Membrane ◽  
Pathogenic Bacteria ◽  
Membrane Vesicles ◽  
Multidrug Resistant ◽  
Outer Membrane Vesicles ◽  
Biologically Active ◽  
Gram Negative Bacteria ◽  
Gram Negative

The increasing spread of multidrug-resistant pathogenic bacteria is one of the major threats to public health worldwide. Bacteria can acquire antibiotic resistance and virulence genes through horizontal gene transfer (HGT). A novel horizontal gene transfer mechanism mediated by outer membrane vesicles (OMVs) has been recently identified. OMVs are rounded nanostructures released during their growth by Gram-negative bacteria. Biologically active toxins and virulence factors are often entrapped within these vesicles that behave as molecular carriers. Recently, OMVs have been reported to contain DNA molecules, but little is known about the vesicle packaging, release, and transfer mechanisms. The present review highlights the role of OMVs in HGT processes in Gram-negative bacteria.

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