scholarly journals Microbiological quality of fish grown in wastewater-fed and non-wastewater-fed fishponds in Hanoi, Vietnam: influence of hygiene practices in local retail markets

2007 ◽  
Vol 5 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Nguyen Thi Phong Lan ◽  
Anders Dalsgaard ◽  
Phung Dac Cam ◽  
Duncan Mara

Mean water quality in two wastewater-fed ponds and one non-wastewater-fed pond in Hanoi, Vietnam was ∼106 and ∼104 presumptive thermotolerant coliforms (pThC) per 100 ml, respectively. Fish (common carp, silver carp and Nile tilapia) grown in these ponds were sampled at harvest and in local retail markets. Bacteriological examination of the fish sampled at harvest from both types of pond showed that they were of very good quality (2−3 pThC g−1 fresh muscle weight), despite the skin and gut contents being very contaminated (102−103 pThC g−1 fresh weight and 104−106 pThC g−1 fresh weight, respectively). These results indicate that the WHO guideline quality of ≤1000 faecal coliforms per 100 ml of pond water in wastewater-fed aquaculture is quite restrictive and represents a safety factor of ∼3 orders of magnitude. However, when the fish from both types of pond were sampled at the point of retail sale, quality deteriorated to 102−105 pThC g−1 of chopped fresh fish (mainly flesh and skin contaminated with gut contents); this was due to the practice of the local fishmongers in descaling and chopping up the fish from both types of pond with the same knife and on the same chopping block. Fishmonger education is required to improve their hygienic practices; this should be followed by regular hygiene inspections.

2011 ◽  
Vol 74 (10) ◽  
pp. 1762-1768 ◽  
Author(s):  
MOUNA BOULARES ◽  
LOBNA MEJRI ◽  
MNASSER HASSOUNA

Eighty samples of fresh fish were collected in Tunisia and analyzed for microbial load. Quality and hygienic safety of the meat and intestines of wild and aquacultured fresh fish were determined. The mesophilic aerobic plate count and populations of psychrotrophic lactic acid bacteria (LAB) and other psychrotrophic bacteria ranged from 5.67 to 7.29, 4.51 to 6, and 5.07 to 6.21 log CFU/g, respectively. For all microbiological determinations, bacterial counts were lower in meat than in the intestines of fresh fish. For all samples lower microbial populations were found in most of the wild fish than in the aquacultured fish. No isolates of the pathogenic genera Salmonella and Listeria were detected in any sample. Among the 160 strains of biopreservative psychrotrophic LAB and the 150 strains of spoilage psychrotrophic gram-negative bacteria identified by biochemical and molecular methods, Lactobacillus (six species) and Pseudomonas (six species) predominated. Lactococcus, Leuconostoc, Carnobacterium (C. piscicola and C. divergens), Aeromonas, and Photobacterium were the most common genera, and Lactococcus lactis, Lactobacillus plantarum, Pseudomonas fluorescens, and Aeromonas hydrophila were the most common species. These findings indicate that the microbiological quality of fresh fish in Tunisia can be preserved by controlling pathogenic and psychrotrophic bacteria.


1983 ◽  
Vol 46 (8) ◽  
pp. 733-736 ◽  
Author(s):  
W. L. PAYNE ◽  
A. P. DURAN ◽  
J. M. LANIER ◽  
A. H. SCHWAB ◽  
R. B. READ ◽  
...  

A national survey was conducted of the microbiological quality of three dry ingredients used in beverages and one frozen non-dairy topping obtained at retail markets. Geometric mean aerobic plate counts (APCs) of units examined at 35°C were as follows: 1,313 units of cocoa powder, 6,600 CFU/g; 1,552 units of dry instant chocolate drink mix, 290 CFU/g; 1,559 units of dry non-dairy coffee creamer, 37 CFU/g; and 1,532 units of frozen non-dairy topping, 34 CFU/g. At 30°C, the geometric mean APC was 34 CFU/g for frozen nondairy topping. Geometric means for most probable number determinations of coliform bacteria and Escherichia coli were <3/g for the four products. Geometric mean values for Staphylococcus aureus in three of the products were <10/g; no S. aureus was found in cocoa powder. Geometric mean values for yeasts and molds in dry instant chocolate drink mix and dry nondairy coffee creamer were 8 and 6 CFU/g, respectively.


2010 ◽  
Vol 55 (No. 5) ◽  
pp. 233-241 ◽  
Author(s):  
N. TTopic Popovic ◽  
A. Benussi Skukan ◽  
P. Dzidara ◽  
R. Coz-Rakovac ◽  
I. Strunjak-Perovic ◽  
...  

 Fresh and frozen seafood products (fish, shellfish, crustaceans, molluscs) in wide use in Croatia and typical of the Mediterranean diet, were examined for the presence of microbiological contamination through the winter and summer seasons. Total bacterial counts of aerobic mesophilic bacteria (AB), aerobic psychrophilic bacteria (AP), Salmonella spp., Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, sulphite-reducing clostridia (SRC), Listeria monocytogenes, Vibrio cholerae and V. parahaemolyticus were measured. The microbiological quality of individual samples varied widely between animal species and also between winter/summer seasons regarding total counts of aerobic mesophilic and psychrophilic bacteria. The poorest quality was for (both summer and winter) fish samples, where 66.6 % of fresh and frozen fish were found unacceptable by Croatian standards. The overall prevalence of V. parahaemolyticus was 5%. Its recovery rate was higher in fresh/frozen shellfish in both seasons than in other specimens or other storage/season conditions. Fresh crustaceans sampled in winter demonstrated significantly higher aerobic mesophilic counts than frozen ones. Unacceptable Enterobacteriaceae levels were obtained in 40% of the fresh fish summer samples. The results of this survey constitute an indicator of bacteriological contamination of a variety of seafood. The findings could serve as a basis for future testing of seafood, and possibly as a template for developing a regional/Mediterranean testing scheme on the microbial contamination of seafood in order to establish data with comparative epidemiological and statistical values.


2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Dema Indah Sulistyorini ◽  
Sukiran Al Jauhari ◽  
Siti Surasri

ABSTRACTPetis is a pasta-like food made of fresh fish/shrimp. Petis is usually produced by IRT innon sanitary way. These conditions allow the pollution/contamination of the petis production, eitherby microorganisms, physical objects, or chemicals. This study aims to determine the quality ofpetis production in Socah, Bangkalan, Madura.This research is a descriptive research. Data were collectetd by observations andlaboratory tests. There were 3 petis industries in Socah, where we took samples.The results showed that the quality of petis in physical aspects were not qualified, thechemical aspects were good, and microbiological aspects of two samples were not eligible. Sanitaryconditions of the production-sites were less than 53.4%, but the domestic industry‟s score were68%.The final conclusion is that petis production in Socah Madura is ineligible, on physical andmicrobiological aspect. Only in chemical aspect they were eligible. It is recomended to the peopleto be careful in buying any petis from Socah, test the smell and texture before used for dressing infood.Keywords : Petis, Physical, Chemical and Microbiological Quality.


2014 ◽  
Vol 63 (2) ◽  
pp. 237-243 ◽  
Author(s):  
ROWAIDA KHALIL ◽  
MOHAMED GOMAA

This is a pioneer study in Egypt that provides some assessment of the microbiological quality of conventional and organic leafy green vegetables that constitute an essential component of the Egyptians' daily diet. A total of 380 samples of unpackaged whole conventional and 84 packaged whole organic leafy greens were collected from retail markets in Alexandria, and analyzed for total aerobic mesophilic count (AMC) and total E. coli count (ECC) using the standard spread plate method. Mean AMC values for organic samples were statistically less (p < 0.05) than those of the corresponding conventional samples. Conventional radish and organic parsley samples had the highest AMC of 7.17 and 7.68 log CFU/g respectively, while conventional green cabbage and organic basil had the lowest AMC of 3.63 and 3.23 log CFU/g respectively. The presence of E. coli in 100% of the studied leafy greens was indicative of potential fecal contamination, in view of open and unhygienic environmental and unsanitary handling conditions, as leafy green items are available for sale by street-vendors. Unsatisfactory AMC and ECC levels encountered in the studied samples, warrant future investigations to determine the potential prevalence of foodborne pathogens, and to identify sources of dominating microorganisms, which could make a contribution to the field of food safety.


2013 ◽  
Vol 10 (2) ◽  
pp. 331-338
Author(s):  
MM Hossain ◽  
MN Hassan ◽  
UJ Lithi ◽  
AAKM Nowsad

The fish landed for domestic consumption suffers from serious post harvest loss due to ignorance and negligence of the people involved in the harvest, distribution, processing and trade. Low quality fish is a constraint for food security and public health and is a cause for economic loss. The present study was carried out to identify the cause and to quantify the post harvest loss by a sensory tool. Quality deterioration of some major inland species of wet fish was determined at different steps of distribution channel from the fish farm gate of Mymensingh to Dhaka retail markets in June and July 2010. Five distribution channels i.e. farm gate, transport, arot (commission agent), wholesale and retail markets were studied to find out the deterioration level of the quality of fish. Data were collected by travelling with fish and from the stakeholders in distribution channel through questionnaire interviews. In the distribution channel of wet fish RRA and SWOT analysis were done with fish farmer, arotder, transporter, wholesaler and retailer. The major species studied were Indian major carps (Labeo rohita, Catla catla and Cirrhina mrigala), pangas (Pagasius sutchi), silver carp (Hypopthalmichthys molitrix) and tilapia (Oriochromis niloticus). Different species showed different levels of deterioration of quality at various steps of distribution channels. The quality of fish was found to be changed in between arot and retail market. Inadequate knowledge and practice in handling, icing and container use were found to be the major bottlenecks to keep the fish in acceptable quality.DOI: http://dx.doi.org/10.3329/jbau.v10i2.14926 J. Bangladesh Agril. Univ. 10(2): 331-337, 2012


2016 ◽  
Vol 118 (4) ◽  
pp. 777-794 ◽  
Author(s):  
Phuc Hong Luu ◽  
Michael P Dunne ◽  
Warwick Pearse ◽  
Belinda Davies

Purpose – In order to improve the safety of seafood in the domestic seafood distribution chains (DSDCs) in Vietnam, a better understanding of current hygiene and practices compliance with government regulations is needed. The paper aims to discuss this issue. Design/methodology/approach – Infrastructure conditions and documents related to hygiene procedures of 50 trading places were evaluated using checklists. The hygiene handling practices of 135 fish distributors were also observed by using notational analysis methods. This notational analysis method focused on actions related to microbiological contamination in terms of hand washing or glove changing and the cleaning and sanitising of tools and equipment. Additionally, microbiological quality of 135 samples of raw finfish at points along distribution chains was compared with national standards. Findings – The results indicated that all trading places could be classified as non-compliant or seriously non-compliant with the regulations. The practices of fish distributors were also assessed to be at high risk for contamination of raw fish. The findings showed that approximately 42 and 39 per cent of samples from fishing ports and fish markets, respectively were classified as unacceptable according to the microbiological standards of Vietnam. Research limitations/implications – The present study is limited to research only from fish landing at fishing ports to the distribution to consumers at retail markets. Originality/value – Recommendations for improving food safety in the DSDCs in Vietnam have been developed from the findings of this study and are provided. However, due to similarities with fish distribution chains in other countries, these recommendations may have broader application.


1983 ◽  
Vol 46 (10) ◽  
pp. 901-909 ◽  
Author(s):  
CARLOS ABEYTA

A microbiological survey of 287 (fresh) seafood products from Puget Sound retail markets was conducted over a period of 1 year. The microbiological quality of fresh seafood was high, with only 2.1 % of the samples exceeding the maximum limit for acceptability as suggested by the International Commission on Microbiological Specifications for Foods (ICMSF). The overall microbiological data of positive units given as arithmetic means were: coliforms MPN/g, 199; Escherichia coli MPN/g, 21; coagulase-positive Staphylococcus aureus MPN/g, 66; enterococci/g, 9121; Clostridium perfringens/g, 18; Bacillus cereus/g, 100; and Vibrio parahaemolyticus MPN/g, 3.7. The standard plate count means 1.0 × 103 to 2.5 × 107 colony-forming units (CFU)/g, giving a mean value of 2.0 × 105 CFU/g. The percentages of seafood samples positive for pathogens were S. aureus, 37.6; Yersinia enterocolitica, 3.8; V. parahaemolyticus, 2.8; C. perfringens, 2.4; and B. cereus, 0.7. Vibrio cholerae, Clostridium botulinum, Salmonella and Shigella species were not isolated.


2014 ◽  
Vol 79 (9) ◽  
pp. M1739-M1744 ◽  
Author(s):  
Jee-Hoon Ryu ◽  
Minju Kim ◽  
Eun-Gyeong Kim ◽  
Larry R. Beuchat ◽  
Hoikyung Kim

2003 ◽  
Vol 66 (10) ◽  
pp. 1832-1839 ◽  
Author(s):  
HANNA MIETTINEN ◽  
ANNE ARVOLA ◽  
TIINA LUOMA ◽  
GUN WIRTANEN

The prevalence of Listeria monocytogenes in retail roe, as well as the microbiological and sensory qualities of the roe, were studied for three fish species under three different storage conditions. A total of 147 Finnish rainbow trout (Oncorhynchus mykiss), white fish (Coregonus lavaretus), vendace (Coregonus albula), and burbot (Lota lota) roe samples were bought fresh, frozen, or frozen-thawed from Finnish retail markets. The overall prevalence of L. monocytogenes was 5%; however, the prevalence of the pathogen in fresh roe was 18%. Fresh-bought roe tested positive for Listeria spp. and for L. monocytogenes, respectively, 5 and 20 times as often as did frozen and frozen-thawed roe products combined. The microbiological quality (analyzed as total aerobic heterotrophic bacteria and coliform bacteria) of 78% of the roe samples was unacceptable. Frozen roe samples were found to have the best microbiological quality. According to the results of a sensory evaluation, at least one sensory attribute (appearance, odor freshness, texture, and freshness of taste) was unacceptable for 29% of the roe samples studied. The sensory quality of roe samples bought fresh was better than that of roe samples bought frozen or frozen-thawed. From the results of this study, it is concluded that both the microbiological and the sensory qualities of roe at the retail level need to be improved.


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