Elachi lemon (Citrus limon) peel and pulp: Antioxidant, antimicrobial, anticoagulant activities, bioactive compounds, minerals, and heavy metals

2020 ◽  
Vol 16 ◽  
Author(s):  
Shahad Saif Khandker ◽  
Alamgir Kabir ◽  
Md. Jahid Hasan ◽  
Md. Shohael Ahmed ◽  
Siew Hua Gan ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Anticoagulant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Antioxidant Power

Background: According to a number of previous studies, citrus fruits are rich in phytochemicals, minerals and bioactive compounds. Objective: The aim of this study was to investigate the antioxidant, antimicrobial and anticoagulant activities, phytonutrients, bioactive compounds, mineral and heavy metals in peel and pulp of Elachi lemon (Citrus limon). Methods: Phytochemical screening was performed to estimate the total polyphenol, flavonoid, tannin, reducing sugar, total protein and vitamin C contents. High performance liquid chromatography was performed to determine the concentrations of phenolics and ascorbic acid. Antioxidant activities were evaluated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay and ferric reducing antioxidant power (FRAP) assay. Antibacterial activity was evaluated by disc diffusion method and minimum inhibitory concentration (MIC). Besides estimating heavy metals and minerals, activated partial thromboplastin time (aPTT) and prothrombin time (PT) were observed for detecting anticoagulant activity. Results: In case of antioxidant and antimicrobial activities, pulp was found to be richer. Both the peel and pulp showed similar anticoagulant activities. Among the phytonutrients, protein content was richer in peel that in pulp. Catechin, rutin, tannic, gallic and vanilic acids were detected in peel whereas in pulp quercetin, tannic and gallic acids were found. Pb, Cr, Cd were present in a negligible amount whereas, Co, Cu and Fe content were high in both the peel and pulp. Conclusion: This study exhibits that both the peel and the pulp Citrus limon is a rich source of antioxidant, antimicrobial, anticoagulant, minerals and various bioactive compounds with nutritional values.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

scholarly journals Effect of Vermicompost Application on Bioactive Properties and Antioxidant Potential of MD2 Pineapple Fruits

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 97 ◽  
Author(s):  
Mawiyah Mahmud ◽  
Sujatha Ramasamy ◽  
Rashidi Othman ◽  
Rosazlin Abdullah ◽  
Jamilah Yaacob
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soil Condition ◽  
Chemical Fertilizer ◽  
Ananas Comosus ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Radical Scavenging Properties

Vermicompost is an organic waste produced from earthworms that can enhance the soil condition and is rich with essential plant nutrients, thus increasing produce quality and shelf life. In this study, a one-year field trial was conducted to elucidate the effects of vermicompost supplementation on the composition of bioactive compounds and antioxidant activities of pineapple (Ananas comosus var. MD2) fruits, compared to control and application of chemical fertilizer. Based on the results, pineapple fruits produced from plants supplemented with chemical fertilizer showed the strongest radical scavenging properties against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), followed by vermicompost and control plants. Application of chemical fertilizer and vermicompost also produced fruits with a very high content of chlorophylls and β-carotene compared to control plants. However, the amounts of bioactive compounds present in fruits produced with chemical fertilizer are higher than in fruits produced with vermicompost. Total phenolics content and Ferric Reducing Antioxidant Power (FRAP) reducing power were lowest in fruit extracts produced from pineapple plants supplemented with vermicompost. These results suggested that vermicompost cannot completely replace chemical fertilizer for the production of fruits with a high content of phytoconstituents but could be used as an additional supplement to reduce environmental pollution and ensure agricultural sustainability.

Antioxidant Potential and Bioactive Compounds in Extract and Fractions from the Fruit of Smilax china: an Underutilised Edible Berry

2013 ◽  
Vol 709 ◽  
pp. 883-886
Author(s):  
Hai Lan Huang ◽  
Hai Yuan Wang ◽  
Shu Mei Qiu ◽  
Zong Hua Wang
Keyword(s):  
Bioactive Compounds ◽  
Soluble Fraction ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Total Flavonoids Content ◽  
Dpph Radical Scavenging

The fruit of Smilax china is an edible spherical berry. The content of some bioactive compounds, antioxidant activities in three different assays, and the relationships between these bioactive compounds and antioxidant activity of the extracts derived from the peel of the fruit of were investigated. The results showed that the ethyl acetate-soluble fraction (EAF) was the richest fraction in total phenolic, total flavonoids content. The n-butanol-soluble fraction (BF) had the highest concentration of anthocyanins. EAF and BF showed good DPPH radical-scavenging activity (DRSA) and reducing power (RP), while the crude extract (CE) possessed better hydroxyl radical-scavenging activity (HRSA) than other fractions. There were statistically significant correlations between some antioxidants and antioxidant activities.

Evaluation of Free Radical Scavenging Activity and Reducing Power of Careya arborea Roxb.

2019 ◽  
Vol 886 ◽  
pp. 86-91
Author(s):  
Wanvisa Ninlaor ◽  
Thaksaorn Thongom ◽  
Chawapon Phoomphong ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Active Substances

Antioxidant plays an important role in inhibiting and scavenging radicals, thus providing protection for human against infection and degenerative diseases. The aim of this study was to evaluate the effect of the solvent on the extraction of the antioxidant compounds from Careya arborea (CA) Roxb. The leaves of CA were extracted by using water, ethanol, methanol, and chloroform. The total phenolic content of the extracts was determined by the Folin-Ciocalteu reagent and their antioxidant activity was measured using 2, 2-diphenyl-1 picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The antioxidant activities of the extracts for DPPH and FRAP were in the order: water > methanol > ethanol > chloroform extract. According to the results, water is the most suitable solvent for extraction of active substances with antioxidant activities.

scholarly journals Chemical Compositions and Anti-Skin-Ageing Activities of Origanum vulgare L. Essential Oil from Tropical and Mediterranean Region

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1101 ◽  
Author(s):  
Natnaree Laothaweerungsawat ◽  
Jakkapan Sirithunyalug ◽  
Wantida Chaiyana
Keyword(s):  
Essential Oil ◽  
Mediterranean Region ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Origanum Vulgare ◽  
Antioxidant Power

Origanum vulgare L. has been used as a culinary ingredient worldwide. This study revealed the cosmeceutical potential of O. vulgare essential oil as a skin-ageing retardant. The O. vulgare essential oil from a highland area of a tropical country (HO), obtained by hydrodistillation was investigated and compared to a commercial oil from the Mediterranean region (CO). Their chemical compositions were investigated by gas chromatography–mass spectrometry. Antioxidant activities were investigated by ferric reducing antioxidant power, 1,1-diphenyl-2-picrylhydrazyl, and ferric thiocyanate assay. Anti-skin-ageing activities were determined by means of collagenase, elastase, and hyaluronidase inhibition. Carvacrol was the major component in both oils, but a higher amount was detected in HO (79.5%) than CO (64.6%). HO possessed comparable radical scavenging activity to CO (IC50 = 1.8 ± 0.8 mg/mL) but significantly higher lipid peroxidation inhibition (38.0 ± 0.8%). Carvacrol was remarked as the major compound responsible for the reducing power of both oils. Interestingly, HO possessed significant superior anti-skin-ageing activity than ascorbic acid (P < 0.01), with inhibition against collagenase, elastase, and hyaluronidase of 92.0 ± 9.7%, 53.1 ± 13.3%, and 16.7 ± 0.3%, at the concentration of 67, 25, and 4 µg/mL, respectively. Since HO possessed comparable anti-hyaluronidase activity to CO and superior anti-collagenase and anti-elastase (P < 0.01), HO was suggested to be used as a natural skin-ageing retardant in a cosmetic industry.

scholarly journals Evolution of antioxidant properties of orange beverage during storage: Effects of time and temperature

2021 ◽  
Vol 25 (2) ◽  
pp. 293-300
Author(s):  
Ouahiba Soufi ◽  
Mostapha Bachir-Bey ◽  
Saida Kessi ◽  
Djamila Gani ◽  
Nourredine Touati
Keyword(s):  
Bioactive Compounds ◽  
Storage Temperature ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Duration Of Storage ◽  
Antioxidant Parameters ◽  
Storage Effects

Abstract Fruit beverages are the most consumed refreshing products worldwide. The quality of these drinks is not only influenced by the initial composition of the product but also by conditions and duration of storage. The purpose of the present investigation was to evaluate the incidence of two conservation parameters (temperature and time) on some bioactive compounds (phenolic, flavonoid, and carotenoid contents) and antioxidant capacity, measured by free radical scavenging activity (RSA) and ferric reducing power (FRP), on orange beverage during storage. The industrial drinking product freshly procured were subjected to storage under variable temperatures (5, 25, 35, and 45 °C) then analyses of bioactive compounds contents and antioxidant activity were performed periodically (0, 10, 20, and 30 days). The results showed that the initial orange beverage was endowed with interesting phenolic compound contents and considerable antioxidant potential. In the exception of 5 °C, which provided steady antioxidant parameters, all other temperatures induced decreases of analyzed phytochemicals and antioxidant activities proportionally with raising of storage temperature. The duration of storage for the temperatures 25, 35, and 45 °C also affected negatively antioxidant parameters in a proportional manner to time. The orange beverage was an interesting natural source of bioactive compounds but the duration of storage in relation to temperature must be chosen properly.

scholarly journals Effect of steaming and frozen storage on polyphenol content and antioxidant properties of Mangifera odorata (Kuini) pulp

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 313-321
Author(s):  
Norra I. ◽  
Saiful Bahri S. ◽  
Hadijah H. ◽  
Norhartini A.S.
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Frozen Storage ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Significant Difference

Mangifera odorata (kuini) is a potential source of antioxidant. As a seasonal fruit and easily perishable after harvesting, production of kuini pulp was a common practice to overcome this problem. Steaming treatment were involved in the production of kuini pulp before being stored at -18°C. These thermal treatment and storage are challenging due to the fact that it can induce changes of antioxidants properties of kuini pulp. Thus, this study evaluated the effect of steaming processing and frozen storage on the total phenolic content (TPC) and antioxidant activity (AOA) measured by ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activity (RSA) method of M. odorata (kuini) pulps. Overall, a significant difference was observed for TPC and AOA for steaming kuini pulp compared to control samples. Steaming processing significantly increased the TPC and AOA of the kuini pulps (p < 0.05). Significant relationships (p < 0.05) were identified between total phenolics and total antioxidant activities. Storage at -18°C significantly affected the TPC and AOA of the frozen kuini pulp samples. After 12 months storage of all frozen kuini pulp samples, phenolics content was decreased up to 34%; the reducing power value decreased up to 50%, and DPPH RSA decreased up to 35% of the initial value. Steam blanching is recommended to enhance the phenolic content and the antioxidant but, frozen storage at -18°C cannot preserve the phenolic content and the antioxidant of kuini pulp.

Biological Activities of Carlina Oxide Isolated from the Roots of Carthamus caeruleus

2020 ◽  
Vol 10 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Imane Rihab Mami ◽  
Rania Belabbes ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Penicillium Expansum ◽  
Natural Food ◽  
Antioxidant Power

Background: Carthamus caeruleus belongs to the Asteraceae family. The roots are traditionally used as healing agents. They help to heal burns and treat skin diseases. They are also used against joint inflammation and are very effective against diseases such as irritable bowel syndrome for cancer patients. Objectives: The purpose of this work was i) to study the chemical composition of i) the essential oil and hydrosol extract of Carthamus caeruleus, ii) to isolate the major component of both extracts and iii) to evaluate their antioxidant, antifungal and insecticidal activities. Methods: The essential oil and hydrosol extract obtained from the roots were studied by GC and GC/MS. The antioxidant activities were performed using two different methods i) Radical scavenging activity (DPPH) and ii) the Ferric-Reducing Antioxidant Power (FRAP), using BHT as a positive control. Whereas, the antifungal activity of the essential oil and Carlina oxide was investigated against plant fungi. The fumigation toxicity of C. caeruleus essential oil besides Carlina oxide was evaluated against adults of Bactrocera oleae better known as the olive fly. Results: The essential oil and hydrosol extract were mainly represented by acetylenic compounds such as carline oxide and 13-methoxy carline oxide. Carlina oxide was isolated and identified by 1H and 13C NMR spectroscopic means. The results showed that Carlina oxide presented interesting antioxidant and antifungal properties, while C. caeruleus root essential oil had better insecticidal activity. Furthermore, Carlina oxide has demonstrated promising in vivo antifungal activity to control infection of apples by Penicillium expansum. Conclusion: Carlina oxide can be used as a natural food preservative and alternative to chemical fungicides to protect stored apple against Penicillium expansum.

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

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