scholarly journals Effect of Vermicompost Application on Bioactive Properties and Antioxidant Potential of MD2 Pineapple Fruits

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 97 ◽  
Author(s):  
Mawiyah Mahmud ◽  
Sujatha Ramasamy ◽  
Rashidi Othman ◽  
Rosazlin Abdullah ◽  
Jamilah Yaacob
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soil Condition ◽  
Chemical Fertilizer ◽  
Ananas Comosus ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Radical Scavenging Properties

Vermicompost is an organic waste produced from earthworms that can enhance the soil condition and is rich with essential plant nutrients, thus increasing produce quality and shelf life. In this study, a one-year field trial was conducted to elucidate the effects of vermicompost supplementation on the composition of bioactive compounds and antioxidant activities of pineapple (Ananas comosus var. MD2) fruits, compared to control and application of chemical fertilizer. Based on the results, pineapple fruits produced from plants supplemented with chemical fertilizer showed the strongest radical scavenging properties against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), followed by vermicompost and control plants. Application of chemical fertilizer and vermicompost also produced fruits with a very high content of chlorophylls and β-carotene compared to control plants. However, the amounts of bioactive compounds present in fruits produced with chemical fertilizer are higher than in fruits produced with vermicompost. Total phenolics content and Ferric Reducing Antioxidant Power (FRAP) reducing power were lowest in fruit extracts produced from pineapple plants supplemented with vermicompost. These results suggested that vermicompost cannot completely replace chemical fertilizer for the production of fruits with a high content of phytoconstituents but could be used as an additional supplement to reduce environmental pollution and ensure agricultural sustainability.

scholarly journals Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1191
Author(s):  
Mahak Majeed ◽  
Tanveer Bilal Pirzadah ◽  
Manzoor Ahmad Mir ◽  
Khalid Rehman Hakeem ◽  
Hesham F. Alharby ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Juglans Regia ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Viscum Album ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

Evaluation of Free Radical Scavenging Activity and Reducing Power of Careya arborea Roxb.

2019 ◽  
Vol 886 ◽  
pp. 86-91
Author(s):  
Wanvisa Ninlaor ◽  
Thaksaorn Thongom ◽  
Chawapon Phoomphong ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Active Substances

Antioxidant plays an important role in inhibiting and scavenging radicals, thus providing protection for human against infection and degenerative diseases. The aim of this study was to evaluate the effect of the solvent on the extraction of the antioxidant compounds from Careya arborea (CA) Roxb. The leaves of CA were extracted by using water, ethanol, methanol, and chloroform. The total phenolic content of the extracts was determined by the Folin-Ciocalteu reagent and their antioxidant activity was measured using 2, 2-diphenyl-1 picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The antioxidant activities of the extracts for DPPH and FRAP were in the order: water > methanol > ethanol > chloroform extract. According to the results, water is the most suitable solvent for extraction of active substances with antioxidant activities.

Elachi lemon (Citrus limon) peel and pulp: Antioxidant, antimicrobial, anticoagulant activities, bioactive compounds, minerals, and heavy metals

2020 ◽  
Vol 16 ◽  
Author(s):  
Shahad Saif Khandker ◽  
Alamgir Kabir ◽  
Md. Jahid Hasan ◽  
Md. Shohael Ahmed ◽  
Siew Hua Gan ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Anticoagulant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Antioxidant Power

Background: According to a number of previous studies, citrus fruits are rich in phytochemicals, minerals and bioactive compounds. Objective: The aim of this study was to investigate the antioxidant, antimicrobial and anticoagulant activities, phytonutrients, bioactive compounds, mineral and heavy metals in peel and pulp of Elachi lemon (Citrus limon). Methods: Phytochemical screening was performed to estimate the total polyphenol, flavonoid, tannin, reducing sugar, total protein and vitamin C contents. High performance liquid chromatography was performed to determine the concentrations of phenolics and ascorbic acid. Antioxidant activities were evaluated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay and ferric reducing antioxidant power (FRAP) assay. Antibacterial activity was evaluated by disc diffusion method and minimum inhibitory concentration (MIC). Besides estimating heavy metals and minerals, activated partial thromboplastin time (aPTT) and prothrombin time (PT) were observed for detecting anticoagulant activity. Results: In case of antioxidant and antimicrobial activities, pulp was found to be richer. Both the peel and pulp showed similar anticoagulant activities. Among the phytonutrients, protein content was richer in peel that in pulp. Catechin, rutin, tannic, gallic and vanilic acids were detected in peel whereas in pulp quercetin, tannic and gallic acids were found. Pb, Cr, Cd were present in a negligible amount whereas, Co, Cu and Fe content were high in both the peel and pulp. Conclusion: This study exhibits that both the peel and the pulp Citrus limon is a rich source of antioxidant, antimicrobial, anticoagulant, minerals and various bioactive compounds with nutritional values.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

scholarly journals Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5337 ◽  
Author(s):  
Chanikan Sonklin ◽  
Natta Laohakunjit ◽  
Orapin Kerdchoechuen
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Peptide Chain ◽  
Antioxidant Power ◽  
Meal Protein ◽  
Ferric Reducing Antioxidant Power

Background Bioactive peptides can prevent damage associated with oxidative stress in humans when consumed regularly. Recently, peptides have attracted immense interest because of their beneficial functional properties, safety and little or no side effects when used at high concentration. Most antioxidant peptides are small in size, less than 1 kDa, and contains a high proportion of hydrophobic amino acid. Particularly, tyrosine, leucine, alanine, isoleucine, valine, lysine, phenyalanine, cysteine, methionine and histidine in peptide chain exhibited high antioxidant activity. Mungbean meal protein (MMP) is highly abundant in hydrophobic amino acids. It indicated that MMP might be a good source of antioxidants. Therefore, the objectives were to optimize the conditions used to generate mungbean meal protein hydrolysate (MMPH) with antioxidant activity from bromelain and to investigate the antioxidant activities of different molecular weight (MW) peptide fraction. Methods Response Surface Methodology (RSM) was used for screening of the optimal conditions to produce MMPH. After that MMPH was fractionated using ultrafiltration membranes with different MW distributions. Crude-MMPH and four fractions were investigated for five antioxidant activities: 2,2,1-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide, ferric reducing antioxidant power (FRAP) and metal ion chelation activity. Results The optimal condition to produce the MMPH was 15% (w/w) of bromelain and hydrolysis time for 12 h which showed the greatest DPPH and ABTS radical scavenging activity. After mungbean protein from optimal condition was separated based on different molecular weight, the DPPH radical scavenging activity was the highest for the F4 (less than 1 kDa) peptide fraction. Metal ion chelating activity was generally weak, except for the F4 that had a value of 43.94% at a protein concentration of 5 mg/mL. The F4 also exhibited high hydroxyl and superoxide activities (54 and 65.1%), but moderate activity for ferric reducing antioxidant power (0.102 mmole Fe2+/g protein) compared to other peptide fractions and crude-MMPH. Molecular weight and amino acid were the main factors that determined the antioxidant activities of these peptide fractions. Results indicated that F4 had strong antioxidant potentials. Discussion The lowest MW fraction (less than 1 kDa) contributed to the highest DPPH, superoxide, hydroxyl and metal chelation activity because influence of low MW and high content of hydrophobic amino acid in peptide chain. Results from this study indicated that MMPH peptides donate protons to free radicals because they had significantly high DPPH value compared to superoxide, hydroxyl and FRAP, which reactions were electron donation. Moreover, MMPH peptides had the ability to inhibit transition metal ions because of highly abundant glutamic acid and aspartic acid in peptide chain.

scholarly journals Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5295
Author(s):  
Xinyu Zhao ◽  
Ruyi Chen ◽  
Yueyue Shi ◽  
Xiaoxi Zhang ◽  
Chongmei Tian ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preparative Hplc ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory

This study aimed to isolate, prepare and identify the main flavonoids from a standardized Smilax glabra flavonoids extract (SGF) using preparative HPLC, MS, 1H NMR and 13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+ radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p < 0.01) and the protein expression of NF-κB p-p65 (p < 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids in S. glabra.

scholarly journals Radical Scavenging and Antioxidant Activities of Essential Oil Components – An Experimental and Computational Investigation

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Farukh S. Sharopov ◽  
Michael Wink ◽  
William N. Setzer
Keyword(s):  
Essential Oil ◽  
Electronic Properties ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Oil Components ◽  
Dpph Radical Scavenging

The antioxidant activities of eighteen different essential oil components have been determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, the 2,2 ’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation assay, and the ferric reducing antioxidant power (FRAP) assay. The phenolic compounds, carvacrol, thymol, and eugenol, showed the best antioxidant activities, while camphor, menthol, and menthone were the least active. The structural and electronic properties of the essential oil components were assessed using density functional theory (DFT) at the B3LYP/6-311++G** level. Correlations between calculated electronic properties and antioxidant activities were generally poor, but bond-dissociation energies (BDEs) seem to correlate with DPPH radical-scavenging activities, and the ferric reducing antioxidant power (FRAP) assay correlated with vertical ionization potentials calculated at the Hartree-Fock/6-311++G** level.

scholarly journals Antibacterial and Antioxidant Activities of Derriobtusone A Isolated fromLonchocarpus obtusus

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mayron Alves Vasconcelos ◽  
Francisco Vassiliepe Sousa Arruda ◽  
Daniel Barroso de Alencar ◽  
Silvana Saker-Sampaio ◽  
Maria Rose Jane Ribeiro Albuquerque ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Test Results ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
E Coli ◽  
Planktonic Growth ◽  
Ferric Reducing Antioxidant Power

This study evaluated the effect of derriobtusone A, a flavonoid isolated fromLonchocarpus obtusus, on two important pathogenic bacteria,Staphylococcus aureusandEscherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, andβ-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth ofS. aureusat 250 and 500 μg/mL; however, it did not have the same activity onE. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) ofS. aureusbiofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass ofE. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibitingβ-carotene oxidation. The compound showed no lethality toArtemiasp. nauplii. In conclusion, derriobtusone A may be an effective molecule againstS. aureusand its biofilm, as well as a potential antioxidant compound with no toxicity.

scholarly journals Evaluation and Comparison of the Antioxidant Activities and Nutritional Composition of Cucurbita maxima and Vigna unguiculata Leaf Extracts

2021 ◽  
pp. 29-39
Author(s):  
K. K. Sha’a
Keyword(s):  
Vigna Unguiculata ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Nutritional Composition ◽  
Leafy Vegetables ◽  
Human Consumption ◽  
Cucurbita Maxima ◽  
Phytochemical Screening ◽  
Antioxidant Power

Antioxidant activities and nutritional composition are essential ingredients normally considered in the choice of vegetables for human consumption. Leafy vegetables in particular, are regarded as protective foods in human diet due to their many health benefits. The aim of this research was to carry out the quantitative phytochemical screening, the antioxidant activities of extracts and determine the nutritional content of Cucurbita maxima and Vigna unguiculata leaves. Quantitative phytochemical screening were conducted using standard techniques. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays were used to determine antioxidant activities of these extracts. Nutritional composition was determined using standard procedures.  Quantitative analysis revealed the phytochemicals in C. maxima and V. unguiculata as; saponins (1.03%, and 1.34%), tannins (3.49% and 2.60%), terpenoids (0.0% and 0.47%), flavonoids (2.81% and 4.11%), alkaloids (5.72% and 3.5%), phenols (4.02% and 3.83%) respectively. There was a significant (p=0.05) difference in radical scavenging activity of ethanol leaf extract of V. unguiculata comprared to C. maxima. In FRAP both plants’ extract revealed a good antioxidant reducing power at 100mg/ml (range 0.40 to 0.5 absorbance) at 700nM. Antioxidant activities of extracts is attributed to their flavonoid and phenolic contents. Proximate analysis revealed the nutrients for C. maxima and V. unguiculata as; crude protein (11.58% and 14.83%), crude fat (0.47% and 0.61%), ash (4.11% and 3.72%), crude fiber (6.95 and 4.68%), moisture (1.03% and 1.38%), carbohydrate (75.86% and 74.78%) respectively. This shows that the leaves are a good source of energy to both humans and animals. Also, both vegetables revealed good percentages of proteins which can be used to compliment other sources of protein.

scholarly journals Antioxidant activity and polyphenol composition of Pistacia terebinthus fruit from Tessala (Western Algeria)

2021 ◽  
Vol 5 (11) ◽  
pp. 30-34
Author(s):  
Nazim Bellifa ◽  
Abdelhak Ismail Benhaddou ◽  
Houssem Eddine Ferkous ◽  
Mohammed Adil Selka ◽  
Houari Toumi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Scavenging Effect ◽  
Coffee Substitute ◽  
Ferric Reducing Antioxidant Power ◽  
Pistacia Terebinthus

Background: Consumption of traditional herbal beverages has been generally increased in the last decades, Terebinth coffee, known as ‘‘menengic coffee’’ in Turkish, is one of the most consumed herbal coffees in Turkey, turpentine tree is one of the components of the Mediterranean bush, particularly in Algeria, known as Betoum el Kiffan is largely used as food and in traditional medicine. Aims: In this study, Total phenol, flavonoid content, and antioxidant activity of three extracts of Pistacia trebinthus fruit growing in Algeria was measured using radical scavenging activity tests and metal-related tests including, ferric-reducing antioxidant power (FRAP). The chemical composition profile of the fruits and the coffee brands was identified by thin-layer chromatography, the effects of roasting method of this fruit was rivaled also. Materials and Methods: The total phenolic content of the extracts was determined using the Folin-Ciocalteu method. All extracts of the terebinth fruits and coffee brands displayed a high DPPH scavenging effect. Results: The results of the ferric-reducing antioxidant power show that the reduction capacity is proportional to the increase in the concentration of the samples. All the extracts of the plant exhibit antioxidant activities lower than those of the reference product besides the infusion extract of the P. terebinthus roasted coffee, which is the most active with an optical density of 1.68 nm at a concentration of 400 μg/mL. The chromatography results show that the various extracts of Pistacia terebinthus fruit carry a large number of polyphenols, in particular the carboxylic acids phenols. Conclusions: The plant can be considered as a coffee substitute and opens up promising avenues for the food and pharmaceutical industry in Algeria. Keywords: Antioxidant, Pistacia terebinthus, Coffee, FRAP, polyphenol.

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