Study on the Interaction of 4'-Hydroxychalcones and their Mannich Derivatives with Calf Thymus DNA by TLC and Spectroscopic Methods, a DNA Cleavage Study

Background: Phenolic Mannich bases derived from hydroxychalcones show remarkable cytotoxic potencies towards cancer cell lines. However, the exact mechanism of action is still partially uncleared. Objective: Interaction of two hydroxychalcones and their Mannich derivatives with calf thymus DNA (ctDNA) has been investigated. Methods: Thin-layer chromatography and UV-Vis spectroscopic method were used for studying the interaction. The binding constant has been determined by UV-Vis spectrophotometric titration. The DNA cleavage activity of the compounds was studied by agarose gel electrophoresis. Results: Interaction of the compounds with ctDNA exhibited relatively high intrinsic binding constant (4-5x104 M-1). The results indicate existence of weak, non-covalent interactions between the investigated derivatives with ctDNA. Some compounds showed a slight DNA cleavage activity with pBR322. Conclusion: The obtained results provide additional knowledge on the previously documented cytotoxicity against tumor cell lines of the hydroxychalcones and their Mannich-derivatives.

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Synthesis, physicochemical investigations, DNA cleavage activity of biogenic zinc oxide nanoparticles and their interaction with Calf-Thymus DNA

Materials Today Proceedings ◽

10.1016/j.matpr.2020.12.742 ◽

2021 ◽

Author(s):

Anindita De ◽

Riya Das ◽

Harsimrut Kaur ◽

Preeti Jain

Keyword(s):

Zinc Oxide ◽

Dna Cleavage ◽

Zinc Oxide Nanoparticles ◽

Calf Thymus Dna ◽

Oxide Nanoparticles ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Calf Thymus

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COMPARATIVE PHYTOCHEMICAL INVESTIGATION, ANTIOXIDANT, ANTICANCER AND DNA CLEAVAGE ACTIVITY OF DIFFERENT KADAM EXTRACTS ON CANCER AND NON CANCER CELL LINES

Era s journal of medical research ◽

10.24041/ejmr2019.128 ◽

2019 ◽

Vol 6 (2) ◽

pp. 39-46

Author(s):

Vishnu Kumar ◽

Anchal Trivedi ◽

Aparna Misra ◽

Tanvir Raza Jafri ◽

Farzana Mahdi ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Dna Cleavage ◽

Cancer Cell Lines ◽

Cleavage Activity ◽

Phytochemical Investigation ◽

Dna Cleavage Activity

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Calorimetric and Spectroscopic Studies to Monitor the Interaction of L-Cysteine Functionalized Selenium Nanoparticles with Calf Thymus DNA

Advanced Science Letters ◽

10.1166/asl.2018.12163 ◽

2018 ◽

Vol 24 (8) ◽

pp. 5624-5628 ◽

Cited By ~ 4

Author(s):

Riju K Thomas ◽

S Prasanth ◽

T. V Vineeshkumar ◽

C Sudarsanakumar

Keyword(s):

Binding Sites ◽

Binding Constant ◽

Spectrophotometric Titration ◽

Spectroscopic Studies ◽

Binding Energies ◽

Selenium Nanoparticles ◽

Calf Thymus Dna ◽

Calf Thymus ◽

Number Of Binding Sites ◽

Ct Dna

The focus of the study is to understand the interaction between calf thymus DNA and L-Cysteine capped Selenium nanoparticles (cys-SeNPs) using spectroscopic and calorimetric techniques. The size of the nanoparticles was confirmed using transmission electron microscope (TEM). Isothermal titration calorimerty (ITC) provided the binding energy, the number of binding sites and the thermodynamic parameters. UV-Vis absorption and fluorescence quenching studies established the number of binding sites and binding constant. Stabilization of the ct-DNA in the presence of cys-SeNPs was determined from thermal melting studies. It is confirmed by the spectrophotometric titration and calorimetric studies that, selenium nanoparticles can form a complex with the double–helical DNA. The binding constant of cys-SeNPs with the DNA from fluorescence study was found to be 2.953 × 106 M−1 and is consistent with the value obtained from ITC experiment. The binding energies along with the binding sites symbolize the crucial role of van der Waals forces and hydrogen bonds in the binding of cys-SeNPs with ct-DNA.

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Synthesis, characterization and DNA cleavage activity of nickel(II) adducts with aromatic heterocyclic bases

Journal of the Serbian Chemical Society ◽

10.2298/jsc1001061b ◽

2010 ◽

Vol 75 (1) ◽

pp. 61-74 ◽

Cited By ~ 5

Author(s):

Surendra Babu ◽

Pitchika Krishna ◽

Hussain Reddy ◽

G.H. Philip

Keyword(s):

Dna Cleavage ◽

Magnetic Moments ◽

Nuclease Activity ◽

Mixed Ligand ◽

Mixed Ligand Complexes ◽

Cleavage Activity ◽

Redox Couples ◽

Dna Cleavage Activity ◽

Calf Thymus ◽

Heterocyclic Bases

Mixed ligand complexes of nickel(II) with 2,4-dihydroxyacetophenone oxime (DAPO) and 2,4-dihydroxybenzophenone oxime (DBPO) as primary ligands, and pyridine (Py) and imidazole (Im) as secondary ligands were synthesized and characterized by molar conductivity, magnetic moments measurements, as well as by electronic, IR, and 1H-NMR spectroscopy. Electrochemical studies were performed by cyclic voltammetry. The active signals are assignable to the NiIII/II and NiII/I redox couples. The binding interactions between the metal complexes and calf thymus DNA were investigated by absorption and thermal denaturation. The cleavage activity of the complexes was determined using double-stranded pBR322 circular plasmid DNA by gel electrophoresis. All complexes showed increased nuclease activity in the presence of the oxidant H2O2. The nuclease activities of mixed ligand complexes were compared with those of the parent copper(II) complexes.

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ANTIPROLIFERATIVE AND DNA CLEAVAGE ACTIVITY OF VARIOUS SOLVENT EXTRACTS OF ACACIA FARNESIANA LINN. POD

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i9.19548 ◽

2017 ◽

Vol 10 (9) ◽

pp. 197

Author(s):

Soul Shekhar Sp ◽

Manjulatha K ◽

Satyanarayan Nd

Keyword(s):

Cell Lines ◽

Antiproliferative Activity ◽

Dna Cleavage ◽

Large Population ◽

Myelogenous Leukemia ◽

Significant Activity ◽

Acacia Farnesiana ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Mcf 7

Objective: Cancer has become the major disease by uncontrolled cell growth effecting large population on the globe. This study, deals with antiproliferative activity of different solvent extracts, viz., n-hexane (S1), dichloromethane (DCM) (S2), and methanol (S3) of Acacia farnesiana pod on four cancer cell lines, viz., chronic myelogenous leukemia (K562), breast cancer (MCF-7), hepatocellular carcinoma (HePG2), colorectal adenocarcinoma (Colo 205), and DNA cleavage activity of the extracts on CT-DNA.Methods: The antiproliferative study was performed by MTT assay and DNA cleavage studies of the solvent extracts (S1, S2, and S3) and its fractions (S4 and S5) was carried out by agarose gel electrophoresis method.Results: The antiproliferative activity results revealed that n-hexane extract (S1) has showed activity against MCF-7 (21.70 %) cell line and methanol extract (S3) against K562 (24.5%) and HePG2 (23.3%) cell lines. The DNA cleavage could be seen at every concentration tested by n-hexane (S1), DCM (S2), and methanol (S3) extracts and significant cleavage was observed at concentrations of 25, 50, and 100 μg by fractions (S4) and (S5) of methanol extract.Conclusion: The results indicated that the extract of A. farnesiana pod (n-hexane and methanol) has antiproliferative properties and the DNA cleavage studies performed on CT-DNA was found that the extracts and its fractions showed significant activity at the concentrations tested.

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DNA-cleavage activity of the iron(II) complex with optically active ligands, meta- and para-xylyl-linked N’,N’-dipyridylmethyl-cyclohexane-1,2-diamine

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2021.127834 ◽

2021 ◽

Vol 36 ◽

pp. 127834

Author(s):

Koichi Kato ◽

Yoshimi Ichimaru ◽

Yoshinori Okuno ◽

Yoshihiro Yamaguchi ◽

Wanchun Jin ◽

...

Keyword(s):

Dna Cleavage ◽

Optically Active ◽

Cleavage Activity ◽

Dna Cleavage Activity

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A YoeB toxin cleaves both RNA and DNA

Scientific Reports ◽

10.1038/s41598-021-82950-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Julia McGillick ◽

Jessica R. Ames ◽

Tamiko Murphy ◽

Christina R. Bourne

Keyword(s):

Dna Cleavage ◽

Divalent Cations ◽

Nuclease Activity ◽

Dose Dependence ◽

Cleavage Activity ◽

Double Stranded Dna ◽

Dna Cleavage Activity ◽

Evolutionarily Conserved ◽

Toxin Protein ◽

Rna And Dna

AbstractType II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.

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