ANTIPROLIFERATIVE AND DNA CLEAVAGE ACTIVITY OF VARIOUS SOLVENT EXTRACTS OF ACACIA FARNESIANA LINN. POD

Objective: Cancer has become the major disease by uncontrolled cell growth effecting large population on the globe. This study, deals with antiproliferative activity of different solvent extracts, viz., n-hexane (S1), dichloromethane (DCM) (S2), and methanol (S3) of Acacia farnesiana pod on four cancer cell lines, viz., chronic myelogenous leukemia (K562), breast cancer (MCF-7), hepatocellular carcinoma (HePG2), colorectal adenocarcinoma (Colo 205), and DNA cleavage activity of the extracts on CT-DNA.Methods: The antiproliferative study was performed by MTT assay and DNA cleavage studies of the solvent extracts (S1, S2, and S3) and its fractions (S4 and S5) was carried out by agarose gel electrophoresis method.Results: The antiproliferative activity results revealed that n-hexane extract (S1) has showed activity against MCF-7 (21.70 %) cell line and methanol extract (S3) against K562 (24.5%) and HePG2 (23.3%) cell lines. The DNA cleavage could be seen at every concentration tested by n-hexane (S1), DCM (S2), and methanol (S3) extracts and significant cleavage was observed at concentrations of 25, 50, and 100 μg by fractions (S4) and (S5) of methanol extract.Conclusion: The results indicated that the extract of A. farnesiana pod (n-hexane and methanol) has antiproliferative properties and the DNA cleavage studies performed on CT-DNA was found that the extracts and its fractions showed significant activity at the concentrations tested.

Download Full-text

COMPARATIVE PHYTOCHEMICAL INVESTIGATION, ANTIOXIDANT, ANTICANCER AND DNA CLEAVAGE ACTIVITY OF DIFFERENT KADAM EXTRACTS ON CANCER AND NON CANCER CELL LINES

Era s journal of medical research ◽

10.24041/ejmr2019.128 ◽

2019 ◽

Vol 6 (2) ◽

pp. 39-46

Author(s):

Vishnu Kumar ◽

Anchal Trivedi ◽

Aparna Misra ◽

Tanvir Raza Jafri ◽

Farzana Mahdi ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Dna Cleavage ◽

Cancer Cell Lines ◽

Cleavage Activity ◽

Phytochemical Investigation ◽

Dna Cleavage Activity

Download Full-text

The synthesis and evaluation of the antitumor and antibacterial activity of two novel oxovanadium complexes

Journal of Chemical Research ◽

10.1177/17475198211045894 ◽

2021 ◽

pp. 174751982110458

Author(s):

Jing Wang ◽

Bin Huang ◽

Liqiang Wang ◽

Guijjuan Jiang ◽

Jianxin Cheng ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Biological Activity ◽

Antitumor Activity ◽

Plasmid Dna ◽

Dna Cleavage ◽

Biofilm Formation ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Mcf 7

Two novel oxovanadium(IV) complexes ([VO(hntdtsc)(BPIP)] and [VO(hntdtsc)(MOPIP)] (hntdtsc = 2-hydroxy-1-naphthaldehydethiosemicarbazone, BPIP = 2-(4-bromophenyl)-imidazo[4,5- f]-1,10-phenanthroline, MOPIP = 2-(4-methoxyphenyl)-imidazo[4,5- f]1,10-phenanthroline), are synthesized and characterized. Subsequently, the Methyl Thiazolyl Tetrazolium (MTT) assay is used to investigate the antitumor activity of the ligand and two complexes in vitro.The results indicate that both complexes could significantly inhibit selected tumor cells (SH-SY5Y, MCF-7, and SK-N-SH). In addition, the antibacterial activity of VO(hntdtsc)(BPIP) against Staphylococcus aureus is further investigated. Interestingly, VO(hntdtsc)(BPIP) can efficiently attenuate S. aureus growth and abrogate α-hemolysin secretion and biofilm formation. The plasmid DNA cleavage activity of both complexes is also investigated. The results suggest that supercoiled plasmid DNA is efficiently cleaved after treatment with each complex, which might contribute to the biological activity of these oxovanadium(IV) complexes.

Download Full-text

Study on the Interaction of 4'-Hydroxychalcones and their Mannich Derivatives with Calf Thymus DNA by TLC and Spectroscopic Methods, a DNA Cleavage Study

The Open Medicinal Chemistry Journal ◽

10.2174/1874104502014010122 ◽

2020 ◽

Vol 14 (1) ◽

pp. 122-131

Author(s):

Zsuzsanna Rozmer ◽

Aline Bernardes ◽

Caridad N. Pérez ◽

Pál Perjési

Keyword(s):

Cell Lines ◽

Dna Cleavage ◽

Binding Constant ◽

Spectroscopic Method ◽

Spectrophotometric Titration ◽

Mannich Bases ◽

Calf Thymus Dna ◽

Cleavage Activity ◽

Dna Cleavage Activity ◽

Calf Thymus

Background: Phenolic Mannich bases derived from hydroxychalcones show remarkable cytotoxic potencies towards cancer cell lines. However, the exact mechanism of action is still partially uncleared. Objective: Interaction of two hydroxychalcones and their Mannich derivatives with calf thymus DNA (ctDNA) has been investigated. Methods: Thin-layer chromatography and UV-Vis spectroscopic method were used for studying the interaction. The binding constant has been determined by UV-Vis spectrophotometric titration. The DNA cleavage activity of the compounds was studied by agarose gel electrophoresis. Results: Interaction of the compounds with ctDNA exhibited relatively high intrinsic binding constant (4-5x104 M-1). The results indicate existence of weak, non-covalent interactions between the investigated derivatives with ctDNA. Some compounds showed a slight DNA cleavage activity with pBR322. Conclusion: The obtained results provide additional knowledge on the previously documented cytotoxicity against tumor cell lines of the hydroxychalcones and their Mannich-derivatives.

Download Full-text

DNA-cleavage activity of the iron(II) complex with optically active ligands, meta- and para-xylyl-linked N’,N’-dipyridylmethyl-cyclohexane-1,2-diamine

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2021.127834 ◽

2021 ◽

Vol 36 ◽

pp. 127834

Author(s):

Koichi Kato ◽

Yoshimi Ichimaru ◽

Yoshinori Okuno ◽

Yoshihiro Yamaguchi ◽

Wanchun Jin ◽

...

Keyword(s):

Dna Cleavage ◽

Optically Active ◽

Cleavage Activity ◽

Dna Cleavage Activity

Download Full-text

A YoeB toxin cleaves both RNA and DNA

Scientific Reports ◽

10.1038/s41598-021-82950-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Julia McGillick ◽

Jessica R. Ames ◽

Tamiko Murphy ◽

Christina R. Bourne

Keyword(s):

Dna Cleavage ◽

Divalent Cations ◽

Nuclease Activity ◽

Dose Dependence ◽

Cleavage Activity ◽

Double Stranded Dna ◽

Dna Cleavage Activity ◽

Evolutionarily Conserved ◽

Toxin Protein ◽

Rna And Dna

AbstractType II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.

Download Full-text