Efficacy of MTA Modified by Nanosilver for the Prevention of Coronal Leakage

Background: Numerous materials have been introduced as coronal barriers, however, they have shown various degrees of microleakage. Therefore, attempts are undertaken to introduce more reliable materials with the potential to provide a long-term coronal seal. Introduction: This in vitro study aimed to assess the efficacy of gray ProRoot Mineral Trioxide Aggregate (MTA) modified by a suspension of silver nanoparticles (AgNPs) as an orifice plug using a bacterial leakage method. Methods: 40 extracted human teeth were prepared and filled (except for the negative control group) using the lateral compaction technique. The coronal 2 mm of the root filling was removed in the experimental groups. The teeth were randomly divided into two experimental (n = 15) and two control (n = 5) groups. In the experimental group 1 (MTA group) and the experimental group 2 (Ag-MTA group), MTA modified by a suspension of AgNPs was used as an orifice plug. In the negative control group, the entire root surfaces were covered with two layers of nail varnish. In the positive control group, the root canals were filled with a single gutta-percha cone without a sealer and no orifice plug. Bacterial leakage was assessed using a two-chamber system. The teeth were incubated at 37°C and 100% humidity for 120 days, and human saliva was added to the samples every 3 days. Bacterial microleakage was assessed by daily monitoring and observating the turbidity of the Brain Heart Infusion (BHI) broth. The data were analyzed using the log-rank test. Results: All positive controls showed contamination after 5 days. None of the negative controls were contaminated during the experiment. The Ag-MTA group demonstrated a significantly better coronal seal than the MTA group (p = 0.031). Conclusion: Gray ProRoot MTA modified by AgNPs has the potential for being used as an orifice plug in endodontically treated teeth.

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In Vitro Comparison of MTA and BC RRM-Fast Set Putty as Retrograde Filling Materials

10.21203/rs.3.rs-1099531/v1 ◽

2021 ◽

Author(s):

Carla Arvelaiz ◽

Andreina Fernandes ◽

Veronica Graterol ◽

Katiuska Gomez ◽

Jose Francisco Gomez-Sosa ◽

...

Keyword(s):

Brain Heart Infusion ◽

Bacterial Count ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Material ◽

Human Teeth ◽

Retrograde Filling ◽

Significant Difference

Abstract Background: To compare, in vitro, the bioceramic materials (MTA and BC RRM-fast set putty) capacity to prevent microleakage of Enterococcus faecalis over time. Methods: An experimental design was made with forty extracted human teeth, coronally cut, and prepared to be placed in a leakage system under sterile conditions. They were randomly divided into an experimental group: thirty teeth (fifteen for retrograde filling material MTA and BC RRM-fast set putty, respectively) and a control group: ten teeth (five positive control, five negative control). The 3 mm root-ends were submerged in a brain-heart infusion broth with a red phenol indicator. The coronal access of each sample was inoculated with E. faecalis every seven days to maintain bacterial viability. The lower chamber was evaluated daily for 30 days to observe the turbidity of the culture medium and establish the presence and day of the filtration. Calculation of the colony-forming units (CFU) was performed for each leaked sample. Results: Of the total samples prepared for each type of bioceramic material, leaked 60.0% (9/15) of the MTA group and 40.0% (6/15) of the BC RRM-fast set putty group. All positive controls filtered on the first day of evaluation, while 20% (1/5) of the negative control leaked in the second week. There was no significant difference in leakage between the bioceramic material types, nor concerning the bacterial count and the type of cement used (p = 0.101). Conclusions: This study suggests that BC RRM-fast set putty and MTA have a similar sealing capacity when used as a retrograde filling material in vitro.

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Estudio in vitro de la erosión dental asociada al chimó / In Vitro Study of Dental Erosion associated to Chimo

Universitas Odontologica ◽

10.11144/javeriana.uo36-76.eved ◽

2017 ◽

Vol 36 (76) ◽

Author(s):

Néstor Febres Giordano ◽

Paola Gilioli Medina ◽

Daniela Olávez Cepeda ◽

Carlos Omaña Cepeda ◽

Eduvigis Solórzano Navarro

Keyword(s):

Color Change ◽

Negative Control Group ◽

Dental Erosion ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Experimental Group

ABSTRACT. Background: In Venezuela, chimó is a blackish goo derived from tobacco leaf. Its consumption has been associated with systemic and oral diseases. Purpose: To identify in vitro dental erosion associated to chimó exposure. Methods: The sample consisted of 30 teeth that were assigned 10 to the negative control group, another 10 to the positive control group, and 10 to the experimental group, which were exposed respectively to artificial saliva, Coca-Cola® light, and chimó for 20 weeks. Tooth surfaces were analyzed clinically and structurally, the latter through scanning electron microscope. Results: Clinical observations showed surface color change in the negative control group; color change and surface gloss loss in the positive control group; color and surface changes in the experimental group (p=0.000). Ultrastructural analysis showed no enamel surface alterations in the negative control group. Acid etching scale values were 4 for the positive control group and 3-4 for the experimental group. The latter had signs of enamel erosion. In terms of severity of damage, the experimental group was type 1, that is, enamel loss without involving dentin. Conclusion: Under these experimental conditions, chimó caused clinical and ultrastructural enamel surface loss with type 3-4 erosion patterns, and type 1 severity.

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In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

10.21203/rs.3.rs-1017752/v1 ◽

2021 ◽

Author(s):

Mehdi Dastorani ◽

Muhammad javad Aliee ◽

Raheleh Halabian ◽

Mostafa Solati ◽

Mohammadsadegh Alemrajabi

Keyword(s):

Repeated Measures ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Gingival Fibroblasts ◽

Periapical Lesions ◽

Ah Plus ◽

Low Cytotoxicity ◽

Endodontic Sealers

Abstract Background: This study aimed to assess the cytotoxicity of four commonly used endodontic sealers namely AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal against human gingival fibroblasts (HGFs). Methods: After culturing the HGFs, they were exposed to the respective sealers in set form and in five different weights, after sterilization. The cytotoxicity of the sealers was evaluated after 1, 3 and 7 days using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by repeated measures ANOVA. Results: After 24 h, all sealers showed low cytotoxicity. However, all sealers in 250 mg and 500 mg weights showed significantly higher cytotoxicity than the negative control group at 72 h, and 7 days (P<0.05) except for AdSeal in 80 mg weight (P>0.05). AH Plus was significantly more cytotoxic than other sealers at 3 and 7 days (P<0.05) while AdSeal had the closest results to the negative control group, and showed significantly higher biocompatibility than other sealers in 250 mg concentration. Conclusion: AdSeal showed the highest biocompatibility while AH Plus had the highest cytotoxicity among the tested sealers. Thus, its application may delay the healing of periapical lesions.

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Cytogenetic biomonitoring of Brazilian dentists occupationally exposed to low doses of X-radiation

Pesquisa Odontológica Brasileira ◽

10.1590/s1517-74912002000300003 ◽

2002 ◽

Vol 16 (3) ◽

pp. 196-201 ◽

Cited By ~ 7

Author(s):

Cintia Kaori Miyaji ◽

Ilce Mara de Syllos Cólus

Keyword(s):

Statistical Tests ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Low Doses ◽

Confounding Factors ◽

X Rays ◽

Occupationally Exposed ◽

Experimental Group ◽

Personal Questionnaire

Exposure to X-rays (ionizing radiation) may cause chromosomal aberrations (CA) in somatic or germinative cells in exposed individuals, and may lead to manifestations of diseases such as cancer. This study was carried out to assess the CA frequency in lymphocytes obtained by means of temporary culture of peripheral blood from dentists in Londrina, Paraná, Brazil, who have worked for more than ten years with X-rays. The results obtained from the experimental group were compared with a matched negative control group, which had never been exposed to X-rays. All individuals, dentists and controls, answered a personal questionnaire, from which a profile of each group was obtained. Slides, prepared after the cultures, were stained with Giemsa, and 100 to 200 metaphase cells were analyzed per individual. CA frequencies and types were registered and statistical tests were not necessary to evaluate the obtained data. The analysis of mitotic index (MI) did not indicate significant differences (p < 0.05) between the group of individuals exposed to X-rays and the control group. The analyzed confounding factors did not influence the results of MI and CA frequencies.

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Comparative SEM evaluation of penetration of adhesive systems in human dentin with a non-rinse conditioner and a self-etching primer

Brazilian Dental Journal ◽

10.1590/s0103-64402004000100004 ◽

2004 ◽

Vol 15 (1) ◽

pp. 19-25 ◽

Cited By ~ 9

Author(s):

Julio Orrico de Aragão Pedra e Cal-Neto ◽

Mauro Sayão de Miranda ◽

Katia Regina Hostílio Cervantes Dias

Keyword(s):

Penetration Depth ◽

Third Molar ◽

Adhesive System ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Human Tooth ◽

Control Group ◽

Human Teeth ◽

Homogeneous Groups

The purpose of this study was to compare the effect of a self-etching primer and a non-rinse conditioner with the effect of a conventional adhesive system on the penetration depth in dentin of human teeth, using scanning electronic microscopy (SEM). Fifteen human third molar teeth were sectioned into 2 pieces. All pieces were flattened with grade 600 to 1200 silicon carbide paper and divided into 6 groups: group 1 - Prime & Bond NT (NT), negative control; group 2 - 37% phosphoric acid + Prime & Bond NT (PANT), positive control; group 3 - Non-rinse Conditioner (NRC) + Prime & Bond NT (NRCNT); group 4 - NRC + Prime & Bond 2.1 (NRCPB); group 5 - NRC + Scotchbond MP (NRCSB); group 6 - Prompt L-Pop (PLP). All teeth were covered with Dyract AP. The tooth fragments were decalcified, and its resin replicas were evaluated by SEM by three previously standardized examiners. The penetration was scored from 0 (no penetration) to 3 (maximum penetration). The Kruskal-Wallis and Mann-Whitney U tests (p<0.05) showed three statistically homogeneous groups: {NT, NRCPB, NRCSB and PLP}, {NRCNT} and {PANT}. The authors concluded that the self-etching primer and the non-rinse conditioner provide a lower penetration depth in human tooth dentin than the conventional adhesive system.

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THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.28435 ◽

2018 ◽

Vol 11 (12) ◽

pp. 411

Author(s):

Ameta Primasari ◽

Minasari Nasution ◽

Nurul Hidayati Arbi ◽

Dini Permata Sari ◽

Mohammad Basyuni

Keyword(s):

Leaf Extract ◽

Brain Heart Infusion ◽

Aggregatibacter Actinomycetemcomitans ◽

Negative Control ◽

Plate Count ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

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Effect of different periodontal ligament simulating materials on the incidence of dentinal cracks during root canal preparation

Journal of Dental Research Dental Clinics Dental Prospects ◽

10.15171/joddd.2018.030 ◽

2018 ◽

Vol 12 (3) ◽

pp. 196-200

Author(s):

Ajita Rathi ◽

Prateeksha Chowdhry ◽

Mamta Kaushik ◽

Pallavi Reddy ◽

Roshni Roshni ◽

...

Keyword(s):

Root Canal ◽

Periodontal Ligament ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

P Value ◽

Root Canal Preparation ◽

Impression Material ◽

Group 1

Background. The present study was undertaken to evaluate the incidence of dentinal cracks during root canal preparation with different periodontal ligament simulating materials in vitro. Methods. Seventy freshly extracted human mandibular first premolars were selected and divided into 7 groups in terms of simulating material: group 1: polyether impression material; group 2: polyvinyl acetate adhesive; group 3: polyvinyl siloxane impression material; group 4: cyanoacrylate adhesive; group 5: epoxy resin adhesive; group 6: positive control, without any periodontal ligament simulation; and group 7: negative control, where neither a periodontal ligament simulating material was used nor canal preparation was carried out. Root canal preparation was carried out in all the groups followed by sectioning of roots at 3 mm, 6 mm and 9 mm. The sections were evaluated under a stereomicroscope at ×2.5 for the presence or absence of cracks. Chi-squared test was used to compare the appearance of defective roots between the different experimental groups. Results. The least number of cracks were found in the negative control group, followed by group 1 where polyether impression material was used for periodontal ligament simulation. The difference was significant with a P-value of 0.002 for coronal sections. Conclusion. Under the limitation of the present study, polyether and polyvinyl siloxane (light body) can both be used for simulation of periodontal ligament.

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Cytotoxicity and Hemolytic Properties of Nano-Hydroxyapatite/Polyetheretherketone Biocomposites

Materials Science Forum ◽

10.4028/www.scientific.net/msf.848.567 ◽

2016 ◽

Vol 848 ◽

pp. 567-572 ◽

Cited By ~ 1

Author(s):

Lin Wang ◽

Shen Hua Song ◽

Zheng Zhi Wu ◽

Li Hong Duan ◽

Chun Bao Wang

Keyword(s):

Negative Control Group ◽

Negative Control ◽

National Standard ◽

Control Group ◽

Culture Solution ◽

Contact Method ◽

Cytotoxicity Test ◽

L929 Cells ◽

Hemolysis Test

In order to evaluate the cytocompatibility and hemolytic properties of n-HA/PEEK biocomposites the nanohydroxyapatite/polyetheretherketone (n-HA/PEEK) biocomposites were successfully prepared. The mechanical properties of the biocomposites were proximal to human bone, at the same time, they had the optimal value with the HA volume content of 5%. The PEEK and n-HA/PEEK biocomposites with different HA content extraction medium was prepared with fresh medium. Simple DMEM culture solution was taken as negative control group. The pure PEEK and 5vol.%, 15vol.%, 30vol.% n-HA/PEEK biocomposites were the testing group. The relative proliferation rate of L929 cells was determined on the 1st, 2nd, 3rd and 6th days with CCK-8 assay. The cytotoxicity of n-HA/PEEK biocomposites were evaluated according to ISO 10993-5: 2009. The L929 cells morphology and growth on the 1st, 2nd, 3rd and 6th days were determined under inverted microscope. The hemolysis test in vitro of n-HA/PEEK biocomposites were evaluated through measuring erythrocyte lysis and ferro-hemoglobin freeing degree with indirect contact method basing on ISO 10993-4:2009. The experimental results showed that the growth and morphology of cells in pure PEEK and n-HA/PEEK biocomposites extraction medium had no difference from negative control group. Cytotoxicity test showed that PEEK and n-HA/PEEK biocomposites did not have obvious toxicity on L929 cells, and the cytotoxicity of these extracts was in grade 0-1. Hemolysis test suggested that PEEK and n-HA/PEEK biocomposites did not have obvious hemolysis reaction, and the hemolysis rate of PEEK and n-HA/PEEK biocomposites were 2.37%, 1.71%, 1.05% and 1.32% respectively, which are less than the national standard (5%). It may be concluded that the n-HA/PEEK biocomposites did not have obvious cytotoxicity and hemolysis reaction, which demonstrated that n-HA/PEEK biocomposites had good cytocompatibility.

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Potential of Cocoa Extract (Theobroma cacao) in Inhibiting Erythrocyte Damage Induced by Physalia utriculus Venom

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v6i3.9661 ◽

2020 ◽

Vol 6 (3) ◽

pp. 157

Author(s):

Adinningtyas Intansari ◽

Al Munawir ◽

Laksmi Indreswari

Keyword(s):

Theobroma Cacao ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Marine Biota ◽

Erythrocyte Lysis ◽

Theobroma Cacao L

Physalia utriculus is one of the invertebrate marine biota that is often found in Indonesia. Some symptoms of venoming due to jellyfish stings cause pain, itching, and hemolysis. In Indonesia, 13 cases of jellyfish stings were reported in 2005-2009 with three people dying in Java, Bali, and Bangka. Cocoa beans (Theobroma cacao L.) contain fat, carbohydrates, proteins, and polyphenol compounds that are useful as antioxidants. Polyphenols in the form of epicathechins, catechins, and procyanidins serve to provide protection to hemolysis. The purpose of this study was to determine the potential of ethanol extract of cacao (Theobroma cacao L.) in inhibiting the damage of erythrocyte induced by Physalia utriculus in vitro. This study used 28 samples of erythrocytes divided into seven groups, namely the normal control group, negative controls, and treatment with cocoa ethanol extract 0.2%, 0.1%, 0.04%, and 0.02%. Each subsequent group induced venom Physalia utriculus. The results showed that the average speed of erythrocyte lysis in the treatment group by giving cocoa ethanol extract 0.2%, 0.1%, 0.04%, and 0.02% respectively (seconds ± standard deviation) was 858,25 ± 94,44; 1.000,5 ± 159,93; 678,5 ± 19,71; and 1.006 ± 159,50. The mean speed of erythrocyte lysis in the negative control group was 1,025 ± 164.63 and the positive control group with the administration of N-Acetylcystein can last up to one hour after administration of venoms. Test for normality and homogeneity shows that data is normally distributed and homogeneous. One Way Annova analysis shows the significance value of p <0.05, then a post hoc analysis test was performed with the Bonferoni method to find out the differences in significance in each group. In this study it can be concluded that the administration of cocoa ethanol extract has no potential to inhibit erythrocyte damage that has been venomed by Physalia utriculus in vitro. Keywords: Physalia utriculus, cacao, erythrocyte damage

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Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v3 ◽

2020 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs. Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm. Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively. Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

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