Development of standardized Cyanthillium cinereum (L.) H.Rob. extract and determination of its biological activities

2021 ◽  
Vol 11 ◽  
Author(s):  
Mafusol Kaji ◽  
Panupong Puttarak
Keyword(s):  
Extraction Method ◽  
Biological Activities ◽  
Herbal Medicines ◽  
Hplc Method ◽  
Extraction Yield ◽  
High Yield ◽  
Herbal Tea ◽  
Frap Assay ◽  
Anti Inflammatory ◽  
Dpph Scavenging

Aim: The development of a suitable standardized Cyanthillium cinereum (L.) H.Rob. extract is an active ingredient in healthcare products. Background: C. cinereum is the herbal tea specified in the Thai National List of Essential (herbal) Medicines (NLEM) as the most efficacious tea for smoking cessation. However, herbal tea is inconvenient, and no standardized C. cinereum extraction method was known. Objectives: The study aims to develop a standardized C. cinereum extract preparation method and determine its biological activities. Methods: Various extraction and fractionation methods were performed in order to optimize a suitable standardized extract. The extraction yield, biological activities, and biomarkers (apigenin and luteolin) of the HPLC method were used to select the most suitable extraction method. Results: The results showed that the microwave-assisted extraction (MAE) method with 75% EtOH was the most suitable method. The MAE method obtained apigenin and luteolin at 0.320±0.029 and 0.487±0.012 mg/g, respectively. MAE showed good anti-inflammatory and antioxidant (DPPH and FRAP assay) activities. Subsequently, the MAE extract was fractionated by the Diaion® HP-20 column in order to obtain the most suitable standardized extract. The 50% plus 75% EtOH fractions showed high apigenin (91.20±1.23 mg/g) and luteolin (167.00±0.49 mg/g) contents and exerted potent bioactivities. The standardized C. cinereum extract presented high effectiveness of NO inhibitory activity with an IC50 of 7.88±3.56 µg/mL, and also exerted DPPH scavenging efficacy with an IC50 value of 8.88±0.17 µg/mL and quercetin equivalent at 137.50±2.20 mg/g by FRAP assay. Conclusion : This study succeeded in developing a high-yield extraction method of standardized C. cinereum extract, with potent antioxidant and anti-inflammatory activities, suitable for various purposes.

scholarly journals Extraction of Nobiletin and Tangeretin From Peels of Shekwasha and Ponkan Using [C2mim][(MeO)(H)PO2] and Centrifugation

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984581 ◽  
Author(s):  
Hitomi Mizuno ◽  
Hibiki Yoshikawa ◽  
Toyonobu Usuki
Keyword(s):  
Organic Solvent ◽  
Organic Compounds ◽  
Efficient Method ◽  
Extraction Method ◽  
Biological Activities ◽  
Extraction Yield ◽  
Citrus Reticulata ◽  
Natural Sources ◽  
Citrus Depressa ◽  
Anti Inflammation

The polymethoxyflavonoids nobiletin and tangeretin are isolated from peels of shekwasha ( Citrus depressa Hayata) and ponkan ( Citrus reticulata Blanco). These natural products possess biological activities, including anticancer and anti-inflammation properties. This report describes an efficient method for the extraction and isolation of nobiletin and tangeretin from citrus peels using 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][(MeO)(H)PO2]) and centrifugation/decantation. The results showed that the extraction yield of nobiletin and tangeretin using [C2mim][(MeO)(H)PO2]/organic solvent (1:1 w/w) was 1.4 to 1.6 times higher than that using organic solvent. This extraction method is expected to be applicable for obtaining other organic compounds from natural sources.

scholarly journals Chemicals Compositions, Antioxidant and Anti-Inflammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Maryem Ben Salem ◽  
Hanen Affes ◽  
Khaled Athmouni ◽  
Kamilia Ksouda ◽  
Raouia Dhouibi ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Reference Group ◽  
Histopathological Examination ◽  
Beta Carotene ◽  
Hplc Method ◽  
Inflammatory Activity ◽  
Frap Assay ◽  
Cynara Scolymus ◽  
Etoh Extract ◽  
Anti Inflammatory

Objective. Artichoke (Cynara scolymus L.) was one of the plant remedies for primary health care. The present study was focused on the determination of chemical composition, antioxidant activities, and anti-inflammatory activity and on analyzing its major bioactive polyphenols by HPLC. Methods. Artichoke Leaves Extracts (ALE) were analyzed for proximate analysis and phytochemical and antioxidant activity by several methods such as DDPH, ABTS, FRAP, and beta-carotene bleaching test. The carrageenan (Carr) model induced paw oedema in order to investigate the anti-inflammatory activity. Identification and quantification of bioactive polyphenols compounds were done by HPLC method. The oxidative stress parameters were determined; CAT, SOD, GSH, MDA, and AOPP activities and the histopathological examination were also performed. Results. It was noted that EtOH extract of ALE contained the highest phenolic, flavonoid, and tannin contents and the strongest antioxidants activities including DDPH (94.23%), ABTS (538.75 mmol), FRAP assay (542.62 umol), and β-carotene bleaching (70.74%) compared to the other extracts of ALE. Administration of EtOH extract at dose 400 mg/kg/bw exhibited a maximum inhibition of inflammation induced by Carr for 3 and 5 hours compared to reference group Indomethacin (Indo). Conclusion. ALE displayed high potential as natural source of minerals and phytochemicals compounds with antioxidant and anti-inflammatory properties.

Optimization of Enzymatic Hydrolytic Extraction Conditions for High Yield Polysaccharide Manufacturing from Liquid Fermented Materials of Hypsizigus Marmoreus

2011 ◽  
Vol 145 ◽  
pp. 154-158
Author(s):  
Pei Sheng Yan ◽  
Li Ya Ma ◽  
Li Xin Cao
Keyword(s):  
Regression Model ◽  
Extraction Method ◽  
Biological Activities ◽  
Uniform Design ◽  
Hot Water ◽  
Water Extraction ◽  
Neutral Protease ◽  
High Yield ◽  
Hot Water Extraction ◽  
Hypsizigus Marmoreus

Polysaccharides extracted from fruiting body and mycelia of higher fungi have been found to show various biological activities. Traditionally, these polysaccharides are manufactured by the hot water extraction method. Low polysaccharide yield is the disadvantage of this method. This paper reports the optimal conditions to manufacture polysaccharide with high-yield from Hypsizigus marmoreus mycelia using enzymatic hydrolytic extraction method. Neutral protease was selected as the most cost efficient among six enzymes. The uniform design was further conducted to optimize the enzymatic hydrolytic extraction conditions, and a regression model was constructed to predict polysaccharide yield. The optimized procedures was that 2% (w/w) of neutral protease was added into hydrolytic solution and incubated for 4 h at 43°C, then extracted one time at 100°C for 1 h. Under optimized procedures, the measured polysaccharide yield was 15.73±0.15 (mg/g), which was very close to the predicted value of 16.65 (mg/g). The results validated the accuracy of the regression model and the optimized procedure. When following the optimized procedures, polysaccharide yield was raised 75.0% over that before optimization, and increased by 122.5% and 104.8% over hot-water extraction and microwave assisted extraction methods respectively. This was the first study to apply uniform design for optimizing high yield manufacture of mycelia polysaccharides by enzymatic hydrolytic extraction. We concluded that enzymatic hydrolytic extraction was a simple, high yield method to manufacture polysaccharides from mushroom mycelia and might be used widely in polysaccharide-derived functional foods production.

Sulfation of Quercitrin, Epicatechin and Rutin by Human Cytosolic Sulfotransferases (SULTs): Differential Effects of SULT Genetic Polymorphisms

Planta Medica ◽  
2021 ◽  
Author(s):  
Xue Mei ◽  
Saud A. Gohal ◽  
Eid S. Alatwi ◽  
Ying Hui ◽  
Chunyan Yang ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Chronic Inflammation ◽  
Genetic Polymorphisms ◽  
Biological Activities ◽  
Herbal Medicines ◽  
Biochemical Basis ◽  
Differential Effects ◽  
Sulfotransferase 1A1 ◽  
Radix Bupleuri ◽  
Anti Inflammatory

AbstractRadix Bupleuri is one of the most widely used herbal medicines in China for the treatment of fever, pain, and/or chronic inflammation. Quercitrin, epicatechin, and rutin, the flavonoids present in Radix Bupleuri, have been reported to display anti-inflammatory, antitumor, and antioxidant biological activities among others. Sulfation has been reported to play an important role in the metabolism of flavonoids. In this study, we aimed to systematically identify the human cytosolic sulfotransferase enzymes that are capable of catalyzing the sulfation of quercitrin, epicatechin, and rutin. Of the thirteen known human cytosolic sulfotransferases, three (cytosolic sulfotransferase 1A1, cytosolic sulfotransferase 1C2, and cytosolic sulfotransferase 1C4) displayed sulfating activity toward quercitrin, three (cytosolic sulfotransferase 1A1, cytosolic sulfotransferase 1A3, and cytosolic sulfotransferase 1C4) displayed sulfating activity toward epicatechin, and six (cytosolic sulfotransferase 1A1, cytosolic sulfotransferase 1A2, cytosolic sulfotransferase 1A3, cytosolic sulfotransferase 1B1, cytosolic sulfotransferase 1C4, and cytosolic sulfotransferase 1E1) displayed sulfating activity toward rutin. The kinetic parameters of the cytosolic sulfotransferases that showed the strongest sulfating activities were determined. To investigate the effects of genetic polymorphisms on the sulfation of quercitrin, epicatechin, and rutin, individual panels of cytosolic sulfotransferase allozymes previously prepared were analyzed and shown to display differential sulfating activities toward each of the three flavonoids. Taken together, these results provided a biochemical basis underlying the metabolism of quercitrin, epicatechin, and rutin through sulfation in humans.

scholarly journals PHYLA NODIFLORA (VERBENACEAE): A REVIEW

2016 ◽  
Vol 2 (1) ◽  
pp. 49
Author(s):  
Bashir Ahmad Chaudhary ◽  
Mehreen Jabeen ◽  
Umair Jillani ◽  
Muhammad Uzair
Keyword(s):  
Secondary Metabolites ◽  
Knee Joint ◽  
Joint Pain ◽  
Biological Activities ◽  
Folk Medicine ◽  
Menstrual Disorders ◽  
Herbal Tea ◽  
Future Studies ◽  
Anti Inflammatory ◽  
Antioxidant Effects

Phyla nodiflora (Verbenaceae) has been used in folk medicine for various ailments such as asthma, bronchitis, knee  joint  pain, gonorrhea, irritation of internal haemorrhoids, cardiopathy, hepatitis and fever. It is known to have various biological activities such as antimicrobial, antitumor, anti-inflammatory, antidiabetic, antimelanogenesis, hepatoprotective and antioxidant effects. Phyla nodiflora is a common ingredient of herbal tea used for the treatment of inflammation, menstrual disorders, and infactious disease. In the present study the plant has been completly reviewed for detection and isolation of secondary metabolites and biological activities which will facilitate the scientists to plan for future studies

scholarly journals Synthesis and Biological Evaluation of Some Novel Quinoline based Chalcones as Potent Antimalarial, Anti-inflammatory, Antioxidant and Antidiabetic Agents

2020 ◽  
Vol 32 (4) ◽  
pp. 959-964
Author(s):  
Nargisbano A. Peerzade ◽  
Shravan Y. Jadhav ◽  
Raghunath B. Bhosale
Keyword(s):  
Antimalarial Activity ◽  
Biological Activities ◽  
13C Nmr Spectroscopy ◽  
Biological Evaluation ◽  
New Drugs ◽  
Antidiabetic Activity ◽  
High Yield ◽  
Excellent Activity ◽  
Anti Inflammatory

The objective of the present study was to synthesize a series of some novel quinoline based methoxy substituted chalcones and to evaluate their in vitro antimalarial, anti-inflammatory, antioxidant and antidiabetic activitites. The quinoline based chalcones was synthesized by condensation of 2-chloro-3-formyl qunoline with various methoxy substituted acetophenone in presence of NaOH. The Claisen-Schmidt condensation gave high yield of quinoline based chalcones. Synthesis of 2-chloro-3-formyl quinoline was carried out by Vilsmeir-Haack reaction on acetanilide and 4-methoxy acetanilide which on cyclization along with formylation give corresponding 2-chloro-3-formyl quinoline. The synthesized compounds were screened for in vitro antimalarial, anti-inflammatory, antioxiadant and antidiabetic activities. The structures of the synthesized compounds were characterized by infrared, 1H NMR and 13C NMR spectroscopy. Compounds 1f and 1h showed highest antimalarial activity even more than standard chloroquine diphosphate. Compound 1a showed excellent activity whereas 1c and 1d showed potent anti-inflammatory activity as compared to standard diclofenac. On the other hand, compounds 1a and 1g showed excellent antioxidant activity for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical while compound 1a showed highest inhibition of nitic oxide free radical (NO•) and compound 1h showed highest inhibition for super oxide radical (SOR) as well as highest antidiabetic activity as compared to standard acarbose. All quinolne based chalcones were synthesized in good yields and showed potential biological activities hence they may be helpful for the designing of new drugs.

scholarly journals Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Gahee Ryu ◽  
Jin Bae Weon ◽  
Woo Seung Yang ◽  
Choong Je Ma
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Herbal Medicines ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Nelumbo Nucifera ◽  
Linear Relationships ◽  
Liquid Chromatographic ◽  
Optimized Conditions

Nelumbo nucifera has a variety of biological activities. So it was importantly used as various herbal medicines since traditional times. A simple, fast, and sensitive high-performance liquid chromatographic (HPLC) method was developed in this study for efficient quality control of N. nucifera. Four different compounds, including neferine, 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol, 1-hydroxy-2-methylpropene, and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol, were simultaneously determined. The four compounds were isolated through a Dionex C18 column by gradient elution with 0.1% TFA-water and methanol. The flow rate was 1.0 mL/min, and the wavelength was detected at 205, 254, 280, and 330 nm. The chromatograms were acquired at 205 nm. The four compounds showed good linear relationships (r2>0.96) over five different concentrations, and an average recovery of the method ranged from 96.27% to 108.78%. Through the analysis validation test and application of the method, the optimized conditions verified that it is efficient to isolate the compounds of N. nucifera seed embryos.

scholarly journals Kaempferol as a Dietary Anti-Inflammatory Agent: Current Therapeutic Standing

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4073 ◽  
Author(s):  
Waqas Alam ◽  
Haroon Khan ◽  
Muhammad Ajmal Shah ◽  
Omar Cauli ◽  
Luciano Saso
Keyword(s):  
Molecular Mechanisms ◽  
Oral Absorption ◽  
Biological Activities ◽  
Herbal Medicines ◽  
Mechanical Trauma ◽  
Active Constituent ◽  
Natural Sources ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Inflammation is a physiological response to different pathological, cellular or vascular damages due to physical, chemical or mechanical trauma. It is characterized by pain, redness, heat and swelling. Current natural drugs are carefully chosen as a novel therapeutic strategy for the management of inflammatory diseases. Different phytochemical constituents are present in natural products. These phytochemicals have high efficacy both in vivo and in vitro. Among them, flavonoids occur in many foods, vegetables and herbal medicines and are considered as the most active constituent, having the ability to attenuate inflammation. Kaempferol is a polyphenol that is richly found in fruits, vegetables and herbal medicines. It is also found in plant-derived beverages. Kaempferol is used in the management of various ailments but there is no available review article that can summarize all the natural sources and biological activities specifically focusing on the anti-inflammatory effect of kaempferol. Therefore, this article is aimed at providing a brief updated review of the literature regarding the anti-inflammatory effect of kaempferol and its possible molecular mechanisms of action. Furthermore, the review provides the available updated literature regarding the natural sources, chemistry, biosynthesis, oral absorption, metabolism, bioavailability and therapeutic effect of kaempferol.

High yield ultrasonication extraction method for Undaria pinnatifida sporophyll and its anti-inflammatory properties associated with AP-1 pathway suppression

LWT ◽  
2015 ◽  
Vol 64 (2) ◽  
pp. 1315-1322 ◽  
Author(s):  
Kyung-Mo Song ◽  
Su Jong Ha ◽  
Jang-Eun Lee ◽  
Soon-Hee Kim ◽  
Yong Ho Kim ◽  
...  
Keyword(s):  
Extraction Method ◽  
Undaria Pinnatifida ◽  
High Yield ◽  
Anti Inflammatory

Pharmacologically potentials of different substituted coumarin derivatives

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Benzene Ring ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Pharmacological Properties ◽  
Coumarin Derivatives ◽  
Wide Range ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Coumarin Compounds

Coumarin and its derivatives are widely spread in nature. Coumarin goes to agroup as benzopyrones, which consists of a benzene ring connected to a pyronemoiety. Coumarins displayed a broad range of pharmacologically useful profile.Coumarins are considered as a promising group of bioactive compounds thatexhibited a wide range of biological activities like anti-microbial, anti-viral,antiparasitic, anti-helmintic, analgesic, anti-inflammatory, anti-diabetic, anticancer,anti-oxidant, anti-proliferative, anti-convulsant, and antihypertensiveactivities etc. The coumarin compounds have immense interest due to theirdiverse pharmacological properties. In particular, these biological activities makecoumarin compounds more attractive and testing as novel therapeuticcompounds.

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