scholarly journals PERBANDINGAN AKTIVITAS SITOTOKSIK EKSTRAK DAN MINYAK ATSIRI RIMPANG Curcuma mangga Val. TERHADAP SEL MCF-7

2021 ◽  
Vol 26 (1) ◽  
pp. 85-94
Author(s):  
Purwanto Purwanto ◽  
Putri Khaerani Cahyaningrum ◽  
Retno Sunarminingsih Sudibyo
Keyword(s):  
Breast Cancer ◽  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Steam Distillation ◽  
Mcf 7

Rimpang Curcuma mangga Val. banyak digunakan sebagai obat herbal antikanker payudara. Penelitian aktivitas sitotoksik terhadap sel kanker payudara banyak dilakukan utamanya untuk minyak atsiri rimpang, dan hanya sedikit penelitian terhadap ekstraknya. Walaupun demikian belum ada yang membandingkan aktivitas sitotoksik dari ekstrak dan minyak atsiri tersebut terhadap sel kanker payudara; meskipun kandungan senyawa keduanya berbeda. Oleh karena itu penelitian ini bertujuan membandingkan aktivitas sitotoksik dari ekstrak dan minyak atsiri rimpang C. mangga Val. secara in vitro terhadap sel kanker payudara MCF7. Ekstrak rimpang dibuat secara maserasi menggunakan pelarut n-heksana; sedangkan minyak atsiri dibuat melalui destilasi uap irisan rimpang selama 5 jam. Uji aktivitas sitotoksik in vitro dilakukan menggunakan metoda MTT Assay. Rendemen minyak dari ekstrak n-heksana rimpang C. mangga Val. adalah 1,15 x 10-2 % sedangkan rendemen minyak atsiri adalah 6,3 x 10-2 %. Hasil uji sitotoksik menghasilkan IC50 ekstrak 106,414 µg/ml (R2=0,9677) dan minyak atsiri 198,557 µg/ml (R2=0,8037). Hal ini menunjukkan bahwa ekstrak rimpang C. mangga Val. lebih sitotoksik terhadap sel kanker payudara MCF-7 daripada minyak atsirinya, karena kandungan ekstrak mayoritas diterpenoid (53,18%) sedangkan minyak atsiri mayoritas monoterpenoid (51,34%).THE COMPARISON BETWEEN THE ACTIVITIES OF CYTOTOXIC EXTRACTS AND ESSENTIAL OILS OF RHIZOME Curcuma mango Val. TOWARD MCF-7 CELLSCurcuma mangga Val. rhizome has been used as herbal anti breast cancer. Researches on cytotoxic activity towards breast cancer cells have been done especially to the rhizome’s essential oil; and only few researches done to the extract. However there is no cytotoxic activity comparation of the extract and essential oil towards breast cancer cells; even tough their substance contents are different. Therefore, this study aimed to compare the cytotoxic activity in vitro of the extract and essential oil of C. mangga Val. rhizomes towards breast cancer cells of MCF-7. The rhizome extract was prepared by maceration using N-hexane; while the essential oil was prepared by steam distillation for 5 hours of the sliced rhizomes. The in vitro cytotoxic test was carried out using MTT Assay. The yield of oil from rhizome extract was 1.15 x 10-2 %; while the yield of essential oil was 6.3 x 10-2 %. The IC50 of extract oil was 106.414 µg/ml (R2=0.9677) and the IC50 of essential oil was 198.557 µg/ml (R2=0.8037). It shows that rhizome extract of C. mangga Val. was more cytotoxic towards MCF-7 than the oil because the majority content of extract were diterpenoids (53.18%) while the oil were monoterpenoids (51.34%).

Structural and In Vitro Biological Studies of Organotin(IV) Precursors; Selective Inhibitory Activity Against Human Breast Cancer Cells, Positive to Estrogen Receptors

2012 ◽  
Vol 65 (12) ◽  
pp. 1625 ◽  
Author(s):  
Vasilis I. Balas ◽  
Christina N. Banti ◽  
Nikolaos Kourkoumelis ◽  
Sotiris K. Hadjikakou ◽  
George D. Geromichalos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
X Ray ◽  
Er Positive ◽  
Normal Human ◽  
Mcf 7

Crystals of Ph3SnCl (1) were grown from a methanol/acetonitrile solution. Compounds [Ph3SnOH]n (2) and [(Ph2Sn)4Cl2O2(OH)2] (3) were crystallized from diethyl ether/methanol/acetonitrile and hot acetone/water solutions respectively, of the white precipitation, formed by adding KOH to solutions of 1 and [Ph2SnCl2] in 1 : 1 and 1 : 2 molar ratios respectively. Complex 1 was characterized by X-ray crystallography. X-ray structure determination of compounds 2 and 3 confirmed the previously reported identities. The molecular structure of 1, reported here, is a new polymorphic form of the known one for Ph3SnCl. Four independent [Ph3SnCl] molecules constitute the crystal structure of 1. The moieties are packed in two pairs in a tail-to-tail arrangement. Complexes 1–3 were evaluated for their in vitro cytotoxic activity (cell viability) against human cancer cell lines: HeLa (human cervical), MCF-7 (breast, estrogen receptor (ER) positive), MDA-MB-231 (breast, ER negative), A549 (lung), Caki-1 (kidney carcinoma), 786-O (renal adenocarcinoma), K1 (thyroid carcinoma), and the normal human lung cell line MRC-5 (normal human fetal lung fibroblast cells) versus, the normal immortalized human mammary gland epithelial cell line MTSV17 with a sulforhodamine B (SRB) assay. The results show potent cytotoxic activity of the complexes against all cell lines used, which was superior to that of cisplatin (CDDP). Compounds 1–3 showed higher activity against breast cancer cells MCF-7 (ER positive) than against of MDA-MB-231 (ER negative). These findings prompted us to search for possible interaction of these complexes with other cellular elements of fundamental importance in cell proliferation. The influence of these complexes 1–3 upon the catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX), as well as their binding affinity towards calf thymus-DNA, were kinetically and theoretically studied.

scholarly journals Efek Sitotoksik Ekstrak Etanolik Ocimum basilicum, L. Pada Sel Kanker Payudara

2019 ◽  
Vol 6 (2) ◽  
pp. 74
Author(s):  
Devi Nisa Hidayati ◽  
Ibrahim Arifin ◽  
Fatimatuz Zahroh ◽  
Lina Wahyuni
Keyword(s):  
Breast Cancer ◽  
Cancer Treatment ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Cytotoxic Test ◽  
Mcf 7

ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan  seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7  ABSTRACT             Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast cancer cells. Basil leaves were extracted using maceration  with ethanol 70%. The cytotoxic test was perfomed using MTT assay with various EEBL concentrations: 1000; 500; 250; 125; 62.5; 31,25 µg/mL. The results showed that IC50 of cytotoxic activity in T47D and MCF-7 was 399.86 µg/ml and 387.76 µg/ml respectively. Keywords—Cytotoxic, Ocinum basilicum L., T47D, MCF-7

Antiproliferative activity of synthesized some new benzimidazole carboxamidines against MCF-7 breast carcinoma cells

2018 ◽  
Vol 73 (3-4) ◽  
pp. 137-145 ◽  
Author(s):  
Cigdem Karaaslan ◽  
Filiz Bakar ◽  
Hakan Goker
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Benzimidazole Derivatives ◽  
Dependent Manner ◽  
Base Pairs ◽  
Mcf 7

AbstractBreast cancer is the most endemic cause of cancer among women in both developed and developing countries. Benzimidazole derivatives exemplify one of the chemical classes that show strong cytotoxic activity especially against breast cancer cells (MCF-7). Aromatic amidine derivatives are known as a group of DNA interactive compounds that bind minor groove of the genome, especially A-T base pairs, and show significant in vitro and in vivo toxicity toward cancer cells. In light of these studies, some new mono/dicationic amidino benzimidazole derivatives were synthesized and evaluated for cytotoxic activity on cultured MCF-7 breast cancer cells. Some of these compounds have strongly inhibited MCF-7 cell viability in a dose-dependent manner compared with clinically used reference compounds, imatinib mesylate and docetaxel. Among them, 4-[(5(6)-bromo-1H-benzimidazole-2-yl)amino]benzene-1-carboxamidine (30) showed the best inhibitory activity with IC50value of 4.6 nM.

Anticancer Potential of Calli Versus Seedling Extracts Derived from Rosmarinus officinalis and Coleus hybridus

2020 ◽  
Vol 21 (14) ◽  
pp. 1528-1538
Author(s):  
Sarah Albogami ◽  
Hadeer Darwish ◽  
Hala M. Abdelmigid ◽  
Saqer Alotaibi ◽  
Ahmed Nour El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Transcriptional Profiling ◽  
Significant Inhibition ◽  
Rosmarinus Officinalis ◽  
Crude Extracts ◽  
Incidence And Mortality ◽  
Mcf 7

Background: In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), exert anti-cancer effects. Objective: We investigated the anticancer properties of methanolic crude extracts of seedlings and calli of Rosmarinus officinalis and Coleus hybridus, two Lamiaceae species. Methods: MCF-7 human breast cancer cells were treated with methanolic crude extracts obtained from plant calli and seedlings generated in vitro, and cell proliferation was evaluated. Transcriptional profiling of the seedling and callus tissues was also conducted. Results: The mRNA expression levels of RA genes were higher in C. hybridus seedlings than in R. officinalis seedlings, as well as in C. hybridus calli than in R. officinalis calli, except for TAT and C4H. In addition, seedling and callus extracts of both R. officinalis and C. hybridus showed anti-proliferative effects against MCF-7 cells after 24 or 48 h of treatment. Discussion: At a low concentration of 10 μg/mL, C. hybridus calli and seedling extracts showed the most significant anti-proliferative effects after 24 and 48 h of exposure (p < 0.01); controls (doxorubicin) also showed significant inhibition, but lesser than that observed with C. hybridus (p < 0.05). Results with R. officinalis callus and seedling extracts did not significantly differ from those with untreated cells. Conclusion: Methanolic extracts of R. officinalis and C. hybridus are potentially valuable options for breast cancer treatment.

In vitro evaluation of estrogenic, antiestrogenic and antitumor effects of amentoflavone

2021 ◽  
pp. 096032712199945
Author(s):  
AT Aliyev ◽  
S Ozcan-Sezer ◽  
A Akdemir ◽  
H Gurer-Orhan
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Antitumor Effects ◽  
Antiestrogenic Activity ◽  
Er Positive ◽  
In Vivo Effects ◽  
Mcf 7

Apigenin, a flavonoid, is reported to act as an estrogen receptor (ER) agonist and inhibit aromatase enzyme. However, amentoflavone, a biflavonoid bearing two apigenin molecules, has not been evaluated for its endocrine modulatory effects. Besides, it is highly consumed by young people to build muscles, enhance mood and lose weight. In the present study, apigenin was used as a reference molecule and ER mediated as well as ER-independent estrogenic/antiestrogenic activity of amentoflavone was investigated. Antitumor activity of amentoflavone was also investigated in both ER positive (MCF-7 BUS) and triple-negative (MDA-MB-231) breast cancer cells and its cytotoxicity was evaluated in human breast epithelial cells (MCF-10A). Our data confirmed ER agonist, aromatase inhibitory and cytotoxic effects of apigenin in breast cancer cells, where no ER mediated estrogenic effect and physiologically irrelevant, slight, aromatase inhibition was found for amentoflavone. Although selective cytotoxicity of amentoflavone was found in MCF-7 BUS cells, it does not seem to be an alternative to the present cytotoxic drugs. Therefore, neither an adverse effect, mediated by an estrogenic/antiestrogenic effect of amentoflavone nor a therapeutical benefit would be expected from amentoflavone. Further studies could be performed to investigate its in vivo effects.

scholarly journals Inhibition of MCF-7 breast cancer cell proliferation by 5alpha-dihydrotestosterone; a role for p21(Cip1/Waf1)

2004 ◽  
Vol 32 (3) ◽  
pp. 793-810 ◽  
Author(s):  
MA Greeve ◽  
RK Allan ◽  
JM Harvey ◽  
JM Bentel
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
S Phase ◽  
Cyclin D3 ◽  
P27 Kip1 ◽  
Mcf 7

Androgens inhibit the growth of breast cancer cells in vitro and in vivo by mechanisms that remain poorly defined. In this study, treatment of asynchronously growing MCF-7 breast cancer cells with the androgen, 5alpha-dihydrotestosterone (DHT), was shown to inhibit cell proliferation and induce moderate increases in the proportion of G1 phase cells. Consistent with targeting the G1-S phase transition, DHT pretreatment of MCF-7 cultures impeded the serum-induced progression of G1-arrested cells into S phase and reduced the kinase activities of cyclin-dependent kinase (Cdk)4 and Cdk2 to less than 50% of controls within 3 days. DHT treatment was associated with greater than twofold increases in the levels of the Cdk inhibitor, p27(Kip1), while p21(Cip1/Waf1) protein levels remained unchanged. During the first 24 h of DHT treatment, levels of Cdk4-associated p21(Cip1/Waf1) and p27(Kip1) were reduced coinciding with decreased levels of Cdk4-associated cyclin D3. In contrast, DHT treatment caused increased accumulation of Cdk2-associated p21(Cip1/Waf1), with no significant alterations in levels of p27(Kip1) bound to Cdk2 complexes. These findings suggest that DHT reverses the Cdk4-mediated titration of p21(Cip1/Waf1) and p27(Kip1) away from Cdk2 complexes, and that the increased association of p21(Cip1/Waf1) with Cdk2 complexes in part mediates the androgen-induced growth inhibition of breast cancer cells.

scholarly journals The in vitro radiosensitizer potential of resveratrol on MCF-7 breast cancer cells

2018 ◽  
Vol 282 ◽  
pp. 85-92 ◽  
Author(s):  
Isabel Cristina da Costa Araldi ◽  
Fernando Primitivo Romero Bordin ◽  
Francine Carla Cadoná ◽  
Fernanda Barbisan ◽  
Verônica Farina Azzolin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mcf 7
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