scholarly journals THYROID FUNCTION OF RATS WITH EXPERIMENTAL AIT AT AN EARLY PERIOD AFTER THE INJECTION OF BIOPREPARATIONS OF CORD BLOOD

2021 ◽  
Vol 78 (4) ◽  
pp. 104-109
Author(s):  
Yuliia Kurylko ◽  
Nataliya Malova ◽  
Larisa Sirotenko
Keyword(s):  
Cord Blood ◽  
Thyroid Function ◽  
Autoimmune Thyroiditis ◽  
Early Period ◽  
Male Rats ◽  
Human Thyroid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Actual Problem ◽  
Autoimmune Thyroid ◽  
Biological Preparation

The development of new methods for the treatment of autoimmune thyroid disease is an actual problem of endocrinology. The aim of research was studying of the cell-free effect biological product of cord blood with immunomodulatory properties "Cryocell-Cryocord " on rats’ thyroid function with AIT simulated at an early period of the study. Simulation of autoimmune thyroiditis was carried out by immunization with a suboperatively isolated human thyroid antigen in combination completing Freund's adjuvant on 30 adult male rats weighing 130 - 150 g. Correction was carried out by parenteral ingection of the "Cryocell-Cryocord". Hormonal research (determination of the content of thyroid hormones and antibodies to thyroglobulin (TG-AT) were effected by using standard test kits for enzyme-linked immunosorbent assay at early stages after exposure (7 days, 1 month). Statistical maintained of the results was carried out using the methods of variation statistics. It was used normal distribution to unpaired Student's test to compare indicators. The difference was considered significant at p<0.05. It has been shown the biological preparation of cord blood "Cryocell - Cryocord" has a positive effect with induced autoimmune thyroiditis. It has been noted the normalizing result of the effected correction on the thyroid function of animals in the early period of the studying. It has been proved after the introduction of the biological preparation "Cryocell - Cryocord" in animals with experimental autoimmune thyroiditis observed the inhibition of the manifestations of autoimmune aggression and the normalization of the functional activity of the thyroid gland. These investigations can be the foundation for the development of a new approach to the treatment of patients.

scholarly journals Effect of Selenium on HLA-DR Expression of Thyrocytes

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Csaba Balázs ◽  
Viktória Kaczur
Keyword(s):  
Beneficial Effect ◽  
Autoimmune Thyroiditis ◽  
Sodium Selenite ◽  
Inhibitory Effect ◽  
Human Thyroid ◽  
Free Oxygen Radicals ◽  
Antioxidative Capacity ◽  
Autoimmune Thyroid ◽  
Hla Dr

Autoimmune thyroid diseases (ATDs) represent the most frequent forms of the organ-specific autoimmune thyroid disorders that result from interaction between genetic and environmental factors. Selenium has been shown to exert a beneficial effect on the autoimmune thyroiditis. In spite of therapeutical effect of selenium on autoimmunity, the mechanism of its action has not been revealed.Objective. To determine whether selenium in vitro thyrocytes cultures are able to influence the HLA-DR molecule expression of human thyrocytes and production of free oxygen radicals.Method. Thyrocytes were prepared from human thyroid gland and cultured in vitro in the presence of interferon-γand sodium selenite. The expression of HLA-DR molecules induced by interferon-γin the presence of sodium selenite of various concentration was measured by fluorescence-activated cell sorter.Results. Selenium has a dose-dependent inhibitory effect on the expression of HLA-DR molecules of thyrocytes induced by interferon-γ. This effect of selenium was in the inverse correlation with antioxidative capacity.Conclusion. Beneficial effect of selenium on autoimmune mechanism is a complex mechanism in which the inhibitory effect on HLA-DR molecule expression and antioxidative capacity are involved into therapy of autoimmune thyroiditis.

The effect of dexamethasone on mucosal immunity of uterine tissue during pregnancy in rat

2019 ◽  
Vol 20 (1) ◽  
pp. 75-84
Keyword(s):  
Early Pregnancy ◽  
Histopathological Examination ◽  
Early Period ◽  
Interleukin 10 ◽  
Treated Group ◽  
Female Rats ◽  
Male Rats ◽  
Mrna Levels ◽  
Uterine Tissue ◽  
Leukocytic Infiltration

Disturbances in early pregnancy immunity affect embryo development, endometrial receptivity, placental development, fetal growth and lead to subfertility, dexamethasone is a synthetic glucocorticoid used for treatment of various complications. Immune cells and cytokines were examined during the early pregnancy in twenty-four female rats and six male rats for mating. Rats were grouped into two group control and dexamethasone treated by a dose of 50µgm/kgm body weight daily starting from one week before mating and persisted for one week after pregnancy. Blood samples were collected from each rat at 5hrs and at 1,3,7 day of pregnancy. Extracted RNA was subjected to real time PCR to determine mRNA levels for immune related genes interleukin1a(IL1A) and interleukin 10(IL10). Histopathological examination was done to uterus in order to detect leukocyte infiltration in uterine tissue. Results showed that significant increase in white blood cell count mainly eosinophil at 5hrs and lymphocyte at three and seven day of pregnancy of dexamethasone treated group. Moreover, TNF, C-reactive protein and progesterone were increased mainly at seven day of pregnancy of dexamethasone treated group. Similarly, interleukin 1alpha and interleukin 10 significantly increased at 5hrs and one day of pregnancy of dexamethasone treated group. In contrast, serum levels of total antioxidant capacity and estrogen were decreased significantly at 5hrs and seven day in dexamethasone treated group. Histopathological examination of uterus revealed leukocytic infiltration especially neutrophil and few eosinophils at five hours and one day of gestation then eosinophil become absent at 3day and seven day of dexamethasone group. Epithelial height and uterine gland diameter significantly increased at 5hrs, three day and seven days of gestation of dexamethasone treated group. The present investigation demonstrated that using of dexamethasone by dose of 50µgm/kgm during early pregnancy had a conflicting impact on some immune cytokines and parameters and may reflect a harmful response of immune system toward early period of pregnancy

scholarly journals Reinterpreting Patterns of Variation in Human Thyroid Function: An Evolutionary Perspective

2020 ◽  
Author(s):  
Sarai Keestra ◽  
Vedrana Högqvist Tabor ◽  
Alexandra Alvergne
Keyword(s):  
Thyroid Function ◽  
Stress Responses ◽  
Reference Intervals ◽  
Biomedical Literature ◽  
Human Thyroid ◽  
Thyroid Disorder ◽  
Emerging Infections ◽  
Multiple Levels ◽  
Interest In Research ◽  
To Receive

Abstract Two hundred million people worldwide experience some form of thyroid disorder, with women being especially at risk. However, why human thyroid function varies between populations, individuals and across the lifespan has attracted little research to date. This limits our ability to evaluate the conditions under which patterns of variation in thyroid function are best understood as ‘normal’ or ‘pathological’. In this review, we aim to spark interest in research aimed at understanding the causes of variation in thyroid phenotypes. We start by assessing the biomedical literature on thyroid imbalance to discuss the validity of existing reference intervals for diagnosis and treatment across individuals and populations. We then propose an evolutionary ecological framework for understanding the phylogenetic, genetic, ecological, developmental and physiological causes of normal variation in thyroid function. We build on this approach to suggest testable predictions for how environmental challenges interact with individual circumstances to influence the onset of thyroid disorders. We propose that dietary changes, ecological disruptions of co-evolutionary processes during pregnancy and with pathogens, emerging infections and exacerbated stress responses can contribute to explaining the onset of thyroid diseases. For patients to receive the best personalised care, research into the causes of thyroid variation at multiple levels is needed.

scholarly journals Evaluation of Pulp Repair after BiodentineTM Full Pulpotomy in a Rat Molar Model of Pulpitis

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 784
Author(s):  
Sandra Minic ◽  
Marion Florimond ◽  
Jérémy Sadoine ◽  
Anne Valot-Salengro ◽  
Catherine Chaussain ◽  
...  
Keyword(s):  
Immunohistochemical Analysis ◽  
Tricalcium Silicate ◽  
Male Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
E Coli ◽  
Inflammatory Conditions ◽  
Tnf Α ◽  
Pulp Inflammation ◽  
Characteristics Analysis ◽  
Pulp Repair

Dental pulp is a dynamic tissue able to heal after injury under moderate inflammatory conditions. Our study aimed to evaluate pulp repair under inflammatory conditions in rats. For this purpose, we developed a rat model of controlled pulpitis followed by pulpotomy with a tricalcium silicate-based cement. Fifty-four cavities were prepared on the occlusal face of the maxillary upper first molar of 27 eight-week-old male rats. E. coli lipopolysaccharides at 10 mg/mL or phosphate-buffered saline PBS was injected after pulp injury. Non-inflamed molars were used as controls. Levels of inflammation-related molecules were measured 6 and 24 h after induction by enzyme-linked immunosorbent assay of coronal pulp samples. Pulp capping and coronal obturation after pulpotomy were performed with tricalcium silicate-based cement. Four and fifteen days after pulpotomy, histological and immunohistochemical analysis was performed to assess pulp inflammation and repair processes. Our results showed significantly higher levels of innate inflammatory proteins (IL-1β, IL-6, TNF-α and CXCL-1) compared with those in controls. Moderate residual inflammation near the capping material was demonstrated by histology and immunohistochemistry, with the presence of few CD68-positive cells. We showed that, in this model of controlled pulpitis, pulpotomy with BiodentineTM allowed the synthesis at the injury site of a mineralized bridge formed from mineralized tissue secreted by cells displaying odontoblastic characteristics. Analysis of these data suggests overall that, with the limitations inherent to findings in animal models, pulpotomy with a silicate-based cement is a good treatment for controlling inflammation and enhancing repair in cases of controlled pulpitis.

scholarly journals 1342 CORD BLOOD THYROID FUNCTION STUDIES IN PREMATURE INFANTS WITH AND WITHOUT RESPIRATORY DISTRESS SYNDROME (RDS)

1981 ◽  
Vol 15 ◽  
pp. 667-667
Author(s):  
Alan H Klein ◽  
Barbara Foley ◽  
Thomas P Foley ◽  
Hugh H Macdonald ◽  
Delbert A Fisher
Keyword(s):  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Cord Blood ◽  
Thyroid Function ◽  
Premature Infants ◽  
Distress Syndrome

scholarly journals Interleukin-6 (IL-6) inhibits thyroid function in the presence of soluble IL-6 receptor in cultured human thyroid follicles.

Endocrinology ◽  
1996 ◽  
Vol 137 (11) ◽  
pp. 4857-4863 ◽  
Author(s):  
K Yamazaki ◽  
E Yamada ◽  
Y Kanaji ◽  
K Shizume ◽  
D S Wang ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Interleukin 6 ◽  
Human Thyroid

STUDIES ON THE EFFECT OF THYROTROPIN ON HUMAN THYROID FUNCTION*

1959 ◽  
Vol 19 (1) ◽  
pp. 28-34 ◽  
Author(s):  
JERZY EINHORN ◽  
LARS-GUNNAR LARSSON
Keyword(s):  
Thyroid Function ◽  
Human Thyroid

Enzymatic deglycosylation of porcine thyroid peroxidase: effects on catalytic activity and immunoreactivity

1991 ◽  
Vol 124 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Egberto G. Moura ◽  
Carmen C. Pazos-Moura ◽  
Naokata Yokoyama ◽  
Martha L. Dorris ◽  
Alvin Taurog
Keyword(s):  
Catalytic Activity ◽  
Molecular Mass ◽  
Antigenic Determinants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Minor Role ◽  
Relative Molecular Mass ◽  
Thyroid Peroxidase ◽  
Autoimmune Thyroid ◽  
Tryptic Fragment ◽  
A Minor

Abstract Thyroid peroxidase is a heme-containing, membrane-bound, glycoprotein enzyme that catalyzes iodination and coupling in the thyroid gland. It is also the antigen for microsomal autoantibodies that are commonly found in the serum of patients with autoimmune thyroid disease. We examined the effect of deglycosylation on the catalytic functions and the immunoreactivity of this enzyme. A highly purified, solubilized, large tryptic fragment of porcine thyroid peroxidase, retaining all of the N-linked glycosylation sites of the native enzyme and displaying full catalytic activity was used. It was deglycosylated by treatment with N-glycanase under nondenaturing conditions. The loss in relative molecular mass after treatment, determined by gel electrophoresis, was about 75% of the estimated molecular weight of the glycan portion of porcine thyroid peroxidase. Lectin blots performed with horseradish peroxidase-conjugated concanavalin A showed a similar loss in relative molecular mass but some residual carbohydrate. The intensity of the carbohydrate stain was consistent with the loss of about 75% of the glycans. Despite this loss, three different assays for catalytic activity of porcine thyroid peroxidase were not significantly decreased. Immunoreactivity measured by immunoblotting and by enzyme-linked immunosorbent assay was also unimpaired. These findings suggest that N-glycanase-sensitive glycans in porcine thyroid peroxidase do not act as antigenic determinants and play a minor role, if any, in catalytic activity and, presumably therefore, in the maintenance of protein conformation.

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