Efeito Antimicrobiano do Alecrim (Rosmarinus officinalis) sobre Cepas de Staphylococcus aureus e Escherichia coli Isoladas de Pacientes de um Hospital Escola do Sul de Minas/Antimicrobial Effect of Rosemary (Rosmarinus officinalis) on Strains of Staphyloc

1970 ◽  
Vol 4 (2) ◽  
pp. 55-63
Author(s):  
Ana Paula Laranjeira Lima ◽  
Emília de Souza Barbosa Grosso ◽  
Gislene Ferreira ◽  
Mariléia Chaves Andrade
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Crude Extract ◽  
Antimicrobial Effect ◽  
Rosmarinus Officinalis ◽  
Antimicrobial Action ◽  
Extract Concentration ◽  
Rosmarinus Officinalis L ◽  
Broth Dilution

Objetivo: Determinar a ação antimicrobiana do extrato bruto de Rosmarinusofficinalis L. (alecrim), sobre cepas de Staphylococcus aureus e Escherichiacoli. Materiais e Métodos: Para obtenção do extrato bruto (EB) foramtrituradas folhas frescas do alecrim e levadas ao percolador com soluçãoalcóolica (etanol 96 GL) por 10/15min, sendo posteriormente filtradas econcentradas em estufa a 65°C, até a evaporação completa do álcool. Foram testadas 35 cepas de Staphylococcus aureus e 35 de Escherichia coli isoladasde pacientes internados no Hospital Escola de Itajubá pelo método de diluiçãoem caldo pela técnica de microdiluição em placa. As concentrações testadasforam: 40; 20; 10; 5; 2,5; 1,25; 0,625 e 0,312mg/ml. Resultados: A inibiçãodo crescimento de S. aureus foi diretamente proporcional à concentração doextrato: a concentração de 40mg inibiu 60% das cepas testadas; 20mg inibiu48,57%, 10mg inibiu 14,28% e a concentração de 5mg mostrou 5,71% de inibição. As demais concentrações não apresentaram inibição. Para aEscherichia coli, apenas as concentrações de 40mg e 20mg do extratoapresentaram capacidade de inibição, com 37,14% e 5,71% das cepas inibidas,respectivamente. Conclusão: Houve uma correlação direta entre aconcentração do extrato e o percentual de cepas inibidas, demonstrando queambas as espécies são inibidas pelo extrato bruto da planta Rosmarinusofficinalis L em maiores concentrações.Palavras-chave: Antimicrobianos. Rosmarinus officinalis L. Fitoterápicos.ABSTRACTObjective: To determine the antimicrobial action of the crude extract ofRosmarinus officinalis L. on strains of Staphylococcus aureus and Escherichiacoli. Materials and methods: In order to obtain the crude extract, freshrosemary leaves were smashed, and taken to percolador with alcoholicsolution (Ethanol 96GL) for 10/15min, being then filtered and concentrated ina incubator at 65° C until complete alcohol evaporation. 35 Staphylococcusaureus and 35 Escherichia coli strains isolated from patients in the UniversityHospital of Itajubá were tested by the method of broth dilution in thetechnique of plate microdilution. The concentrations tested were 40; 20; 10; 5;2,5; 1,25; 0,625; and 0,312mg/mL. Results: The inibition of S.aureus growthwas directly proportional to the concentration of the crude extract: theconcentration of 40mg inhibited 60% of the tested strains; 20mg inhibited48,57%, 10 mg inhibited 14,28% and the concentration of 5mg inhibited5,71%. Other concentrations did not demonstrate inhibition capacity. ForEscherichia coli, only 40mg and 20mg concentrations presented inhibitioncapacity, with 37,14% and 5,71 of strains inhibited. Conclusion: There is adirect correlation between crude extract concentration and the fraction ofinhibited strains, showing that both species are inhibited by the crude extractof Rosmarinus officinalis L in higher concentrations.Keywords: Antimycrobial. Rosmarinus officinalis. Phythotherapeutics

INVESTIGAÇÃO DA AÇÃO ANTIMICROBIANA DO LÁTEX DE MANGABEIRA EM DUAS CEPAS DE BACTÉRIAS CAUSADORAS DE PNEUMONIA

2018 ◽  
Vol 4 ◽  
Author(s):  
Enéas Costa Junior ◽  
Anderson Da Cunha Costa ◽  
Kivia Ludmilla Carvalho Ferreira ◽  
Kelly Maria Rêgo da Silva ◽  
Ester Miranda Pereira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Aureus Strain ◽  
Total Inhibition ◽  
Two Samples ◽  
Hancornia Speciosa

Objective: To evaluate the antimicrobial action of latex of Hancornia speciosa in two strains of bacteria causing Pneumonia. Method: The latex was collected and divided into two samples, a sample diluted in 5 mL of distilled water and dissolved in pure latex. The latex was then submitted to the Minimum Inhibitory Concentration (MIC) test, evaluating the lowest concentration capable of inhibiting the growth of strains of Staphylococcus aureus and Escherichia coli. Results: The Minimum Inhibitory Concentration test showed that the diluted latex had no antimicrobial effect in the Staphylococcus aureus strain and showed total inhibition of Escherichia coli growth. In the latex, in its pure form, showed growth in the first three dilutions tested and total inhibition in the others, behaving equally for both Staphylococcus aureus and Escherichia coli. Conclusion: Latex has antimicrobial action and low toxicity.

scholarly journals Dietary Benefits of Baphia nitida Stem Bark and Antimicrobial Effect on Some Pathogens

2020 ◽  
Vol 63 (3) ◽  
pp. 135-141
Author(s):  
Parker Joshua Elijah ◽  
Bennett Chima Nwangum ◽  
Victor Eshu Okpashi ◽  
Austin Ike Chukwunonyelum ◽  
Kenne Michel Tchimene ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Antimicrobial Therapy ◽  
Crude Extract ◽  
Methylene Chloride ◽  
Antimicrobial Effect ◽  
Stem Bark ◽  
Methanol Fraction

The need to find a broad-based nutritional and antimicrobial therapy gave impetus to this investigation. The crude extracts of Baphia nitida stem bark were used to check for antimicrobial effect on selected micro-organisms for possible nutritional and therapeutic application. The stem bark components were extracted with four solvent systems using maceration. A synthetic drug gentamycin - 80 mg/mL (2 mL) was dissolved in 20 mL of distilled water to obtained 8 mg/mL, was used as a control. The crude extract exhibited an antibacterial effect on gram-positive organisms (Staphylococcus aureus and Bacillus cereus), and gram-negative organism (Pseudomonas aeruginosa, and Escherichia coli) at 2 mg/mL. These showed competitiveness with the 8 mg/mL of the control drug. The normal hexane fraction had an effect on Staphylococcus aureus but showed no effect on other bacteria. Minimum inhibitory concentration (MIC) showed a dose-dependent decrease in the effect for the crude extract. Ethyl acetate and methanol fraction had an effect on Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli at varying concentrations - 20, 10, and 5 mg/mL. the initial screening of the Baphia nitida stem bark revealed alkaloids in the methanol-methylene chloride crude extract and methanol fraction only. Other solvents showed the alkaloids, flavonoids, saponins, terpenoids, glycosides, hydrogen cyanides and steroids respectively. Therefore, the presence of the phytochemicals implicates its antimicrobial effect and the basis for its efficacy as antimicrobial therapy.    

scholarly journals Elaboración de nanopartículas de plata sintetizadas a partir de extracto de hojas de romero (Rosmarinus officinalis L.) y su uso como conservante

La Granja ◽  
2021 ◽  
Vol 35 (1) ◽  
Author(s):  
Lucas Pardo ◽  
Jason Arias ◽  
Patricia Molleda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Rosmarinus Officinalis ◽  
E Coli ◽  
Para Determinar ◽  
Rosmarinus Officinalis L

Las nanopartículas son materiales que pueden llegar a medir entre 1 a 100 nm de longitud, y en la actualidad la propiedad antimicrobiana de las nanopartículas de plata es aprovechada por las industrias para la fabricación de productos de belleza y medicamentos. Las nanopartículas pueden ser sintetizadas a partir de plantas, algas o microorganismos, y también pueden ser obtenidas como productos de combustión. En este estudio se utilizaron extractos de las hojas de romero (Rosmarinus officinalis L.) para la síntesis de nanopartículas de plata (NPs-Ag) con la finalidad de producir un compuesto antimicrobiano para usarse como conservante de frutas. Las NPs-Ag se caracterizaron cualitativa y cuantitativamente mediante análisis fitoquímicos y espectroscopia UV-VIS, presentando una absorción en el rango de 389-418 nm, que corresponde a la resonancia de su plasmón superficial. Además, se empleó la microscopía electrónica de barrido para determinar el tamaño y morfología de las NPs-Ag, observándose una forma esférica de 10 nm de diámetro. Se emplearon dos cepas bacterianas en los ensayos antimicrobianos realizados, la gramnegativa (Escherichia coli) y la grampositiva (Staphylococcus aureus) para comprobar la actividad antimicrobiana de las NPs-Ag.  Para E. coli se obtuvo una mejor actividad antibacteriana con un halo de inhibición de 3,21 mm. Posteriormente se usaron las NPs-Ag en manzanas para determinar su uso como conservante, usando la cera de abeja como control untada en la superficie de las frutas, observándose que las nanopartículas sintetizadas alargaron el tiempo de maduración de la frutas.  

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Antimicrobial Effect of Plasma-Activated Tap Water on Staphylococcus aureus, Escherichia coli, and Candida albicans

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1480
Author(s):  
William Chiappim ◽  
Aline da Graça Sampaio ◽  
Felipe Miranda ◽  
Mariana Fraga ◽  
Gilberto Petraconi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Tap Water ◽  
Antimicrobial Effect ◽  
Gliding Arc ◽  
Oxidation Reduction ◽  
Treatment Conditions ◽  
Oxidation Reduction Potential

In this study, the potential antimicrobial activity of plasma-activated tap water (PAW) was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans. For this, PAW was prepared in a gliding arc plasma system using two treatment conditions: stagnant water and water stirring by a magnetic stirrer, called moving water. Subsequently, their oxidation-reduction potential (ORP), pH, electrical conductivity (σ), and total dissolved solids (TDS) were monitored in different areas of the sample divided according to the depth of the beaker. It was observed that PAW obtained in dynamic conditions showed a more uniform acidity among the evaluated areas with pH 3.53 and ORP of 215 mV. Finally, standardized suspensions of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 10799), and Candida albicans (SC 5314) were treated with PAW, and the reduction of viable cells determined the antimicrobial effect. Our results indicate that the tap water, activated by plasma treatment using gliding arc, is an excellent inactivation agent in the case of Staphylococcus aureus and Escherichia coli. On the other hand, no significant antimicrobial activity was achieved for Candida albicans.

scholarly journals Characterization and antimicrobial activity of vaginal lactobacillus isolate

2011 ◽  
Vol 63 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Gordana Zavisic ◽  
Zeljka Radulovic ◽  
Valentina Vranic ◽  
Jelena Begovic ◽  
L. Topisirovic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Survival Rate ◽  
Bile Salts ◽  
Antimicrobial Effect ◽  
Low Ph ◽  
Probiotic Potential ◽  
Pathogenic Escherichia Coli

The aim of this study was to investigate the probiotic potential of bacteriocin-producing lactobacilli strain Lactobacillus plantarum G2 isolated from the vaginal mucus of healthy women. The antimicrobial effect of G2 was confirmed in the mixed culture with pathogenic Escherichia coli, Staphylococcus aureus, Salmonella abony and Pseudomonas aeruginosa, while bacteriocine activity was detected against S. aureus and S. abony only. The strain showed an excellent survival rate in low pH and in the presence of bile salts. The percentage of adhered cells of L. plantarum G2 to hexadecane was 63.85?2.0 indicating the intermediate hydrophobicity.

scholarly journals Escherichia coli O157 and Non-O157 Isolates Are More Susceptible to l-Lactate than to d-Lactate

2002 ◽  
Vol 68 (9) ◽  
pp. 4676-4678 ◽  
Author(s):  
E. C. McWilliam Leitch ◽  
C. S. Stewart
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Effect ◽  
Membrane Potentials ◽  
Antimicrobial Action ◽  
Escherichia Coli O157

ABSTRACT The antimicrobial effect of l-lactate was much greater than that of d-lactate over a range of concentrations for Escherichia coli O157 and non-O157 strains. Despite this, the intracellular pHs and membrane potentials of l-lactate- and d-lactate-treated cells were similar, suggesting that these factors are not involved in the antimicrobial action of l-lactate.

scholarly journals AVALIAÇÃO DA ATIVIDADE ÓLEOS ESSENCIAIS DE Thimus vulgaris (TOMILHO), Syzygium aromaticum (CRAVO-DA-INDIA) E Rosmarinus officinalis (ALECRIM) E DOS CONSERVANTES BENZOATO DE SÓDIO E SORBATO DE POTÁSSIO EM Escherichia coli E Staphylococcus aureus

2015 ◽  
Vol 33 (1) ◽  
Author(s):  
Angela Aparecida Da Silva ◽  
Márcia Maria Dos Anjos ◽  
Suelen Pereira Ruiz ◽  
Lucimara Bergamo Panice ◽  
Jane Martha Graton Mikcha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Rosmarinus Officinalis ◽  
Thymus Vulgaris ◽  
Syzygium Aromaticum ◽  
E Coli ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Neste trabalho foi avaliada a ação dos óleos essenciais de Thymus vulgaris (tomilho), Syzygium aromaticum (cravo-da-índia) e Rosmarinus officinalis (alecrim) e dos conservantes benzoato de sódio e sorbato de potássio como agentes antimicrobianos. As cepas de Staphylococcus aureus (ATCC 25923) e Escherichia coli (ATCC 25922) foram utilizadas no teste de susceptibilidade antimicrobiana usando-se a técnica de microdiluição em microplaca de 96 poços para avaliação da Concentração Inibitória Mínima (CIM) e, posteriormente, subcultivo em Mueller Hinton Agar para avaliaçãoda Concentração Bactericida Mínima (CBM). As concentrações dos óleos e conservantes sintéticos testados variaram de 15,6 a 1000μg/mL. As microdiluições utilizando inóculos bacterianos nas concentrações de 104 UFC/mL foram incubadas a 37ºC/24h. As CIM para os óleos essenciais de cravo, tomilho e alecrim foram de 550, 650 e >1000μg/mL para E. coli e 550, 800 e 1000μg/mL para S. aureus, respectivamente. No entanto, a ação bactericida dos óleos essenciais do cravo-da-índia e do tomilho foi encontrada apenas em relação a E. coli, na concentração de 550 e 850μg/mL, respectivamente. Para os dois conservantes sintéticos testados, a CIM foi >1000μg/mL, portanto não apresentaram atividade antibacteriana contra os microrganismos testados. Trabalhos futuros deverão ser realizados para verifi car a efi ciência dos antimicrobianos naturais anteriormente citados e para avaliar a possibilidade de serem utilizados na indústria de alimentos.   

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