scholarly journals Removal of lead, mercury and nickel using the yeast Saccharomyces cerevisiae

2014 ◽  
pp. 4141-4149 ◽  
Author(s):  
Cherlys Infante J

ABSTRACTObjective. In this study the biomass of the yeast Saccharomyces cerevisiae was used to remove lead, mercury and nickel in the form of ions dissolved in water. Materials and methods. Synthetic solutions were prepared containing the three heavy metals, which were put in contact with viable microorganisms at different conditions of pH, temperature, aeration and agitation. Results. Both individual variables and the interaction effects influenced the biosorption process. Throughout the experimental framework it was observed that the biomass of Saccharomyces cerevisiae removed a higher percentage of lead (86.4%) as compared to mercury and nickel (69.7 and 47.8% respectively). When the pH was set at a value of 5 the effect was positive for all three metals. Conclusions. pH was the variable that had a greater influence on the biosorption of lead on the biomass of Saccharomyces cerevisiae. The affinity of the heavy metals for the biomass followed the order Pb>Hg>Ni.

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3624 ◽  
Author(s):  
Inga Zinicovscaia ◽  
Nikita Yushin ◽  
Daler Abdusamadzoda ◽  
Dmitrii Grozdov ◽  
Margarita Shvetsova

The performance of the brewer’s yeast Saccharomyces cerevisiae to remove metal ions from four batch systems, namely Zn(II), Zn(II)-Sr(II)-Cu(II), Zn(II)-Ni(II)-Cu(II), and Zn(II)-Sr(II)-Cu(II)-Ba(II), and one real effluent was evaluated. Yeast biosorption capacity under different pH, temperature, initial zinc concentration, and contact time was investigated. The optimal pH for removal of metal ions present in the analyzed solution (Zn, Cu, Ni, Sr, and Ba) varied from 3.0 to 6.0. The biosorption process for zinc ions in all systems obeys Langmuir adsorption isotherm, and, in some cases, the Freundlich model was applicable as well. The kinetics of metal ions biosorption was described by pseudo-first-order, pseudo-second-order, and Elovich models. Thermodynamic calculations showed that metal biosorption was a spontaneous process. The two-stage sequential scheme of zinc ions removal from real effluent by the addition of different dosages of new sorbent allowed us to achieve a high efficiency of Zn(II) ions removal from the effluent. FTIR revealed that OH, C=C, C=O, C–H, C–N, and NH groups were the main biosorption sites for metal ions.


2003 ◽  
Vol 49 (5) ◽  
pp. 336-343 ◽  
Author(s):  
Eduardo V Soares ◽  
Kristel Hebbelinck ◽  
Helena MVM Soares

The decreasing order of toxicity of select heavy metals on the yeast Saccharomyces cerevisiae, in 10 mM MES (2-(N-morpholino)ethanesulfonic acid) pH buffer at pH 6.0, was found to be copper, lead, and nickel. Heavy metal (200 µM) induced a decrease in the number of viable cells by about 50% in the first 5 min for copper and in 4 h for lead, while nickel was not toxic up to a 200 µM concentration over a period of 48 h. Glucose (25 mM) strongly enhanced the toxic effect of 50 µM copper but had little or no effect on the toxicity of 200 µM lead or nickel. Copper, lead, and nickel induced the leakage of UV260-absorbing compounds from cells with different kinetics. The addition of 0.5 mM calcium, before addition of 200 µM copper, showed a protective action against cell death and decreased the release of UV-absorbing compounds, while no effect was observed against lead or nickel toxic effects. Copper complexation capacities of the filtrates of cells exposed for 2 h in 200 µM copper and 24 h in 200 µM lead were 51 and 14 µM, respectively. The implication of the complexation shown by these soluble compounds in the bioavailability of heavy metals is discussed.Key words: copper, lead, nickel, metal bioavailability, toxicity.


2020 ◽  
Vol 115 (11) ◽  
pp. 413-417
Author(s):  
Patrizia Janković ◽  
Renos Spinosi ◽  
Anna Bacardit

With many industries discharging heavy metals into natural water resources, heavy metals have been found to accumulate in various living organisms which can ultimately threaten human life and pose a big threat to the environment. Thus, in the pursuit of a solution to the above mentioned problem, bioaccumulation has emerged as an interesting option for the removal of heavy metals from wastewater. In this paper, the effectiveness of the yeast Saccharomyces cerevisiae in the bioaccumulation of Cr3+ has been tested. Also, different factors influencing Cr3+ uptake have been discussed.  This work has demonstrated that Saccharomyces cerevisiae is an effective Cr3+ biosorbent for tannery wastewater. The conditions of use of this yeast to achieve optimal chromium (III) absorption are: i) when a growth of the biosorbent equivalent to a similar concentration of Cr3+ is obtained, which contains the residual water that needs to be treated; ii) the smaller the biosorbent is the better the biosorption; iii) the uptake of Cr3+ is more efficient when no extra growth medium is added to the wastewater; iv) the longer the exposure period of the yeast to Cr3+ , the bigger the Cr3+ reduction. Since Saccharomyces cerevisiae is an inexpensive, readily available source of biomass, this discovery could be of great use for a low-budget and efficient wastewater treatment system


Jurnal Dampak ◽  
2018 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Shinta Elystia ◽  
Resna Rauda Putri ◽  
Sri Rezeki Muria

Electroplating industry is one of the industries producing liquid waste containing heavy metals. Among the heavy metals contained in the liquid waste is Chromium (Cr) which has a high toxicity. One technology that can be applied to eliminate Cr metal in liquid waste is by the method Biosorption using biomass derived from yeast bread (Saccharomyces cerevisiae). This study aimed to study the ability of bread yeast biomass (saccharomyces cerevisiae) in removing Cr metal on variations in biosorbent weight of 0.25; 0.5 and 0.75 grams, mesh size 80; 100 and 140 and contact time 1; 2; 3 and 4 hours. The results showed that the highest Cr removing efficiency occurred at 0.75 g biosorbent weight at 80 mesh and 4 hours contact time with efficiency of 54.7%. The biosorption process indicates that the adsorption isotherm type obtained is Langmuir isotherms assumed that the layer formed is monolayer with R2 value of 0.8819. The SEM analysis results show the difference of the morphological shape on the sample surface before and after the biosorption process


2016 ◽  
Vol 13 (1) ◽  
pp. 93
Author(s):  
Titin Yulinery ◽  
Ratih M.Dewi

Tes kemampuan adalah salah satu kegiatan penting dalam pengendalian mutu dan jaminan kualitas mikrobiologi laboratorium untuk mengukur kompetensi analis dan analisis uji profisiensi membutuhkan persiapan Model mikroorganisme adalah kualitas standar dan validitas. Mikrobiologi uji kualitas produk kedelai utama diarahkan pada kehadiran Saccharomyces cerevisiae ragi (S. cerevisiae), S. Bailli, S. rouxii dankontaminan bakteri seperti Bacillus dan Deinococcus. Jenis ragi dan bakteri yang terlibat dalam proses dan dapat menjadi salah satu parameter kualitas penting dalam persiapan yang dihasilkan. Jumlah dan viabilitas bakteri dan ragi menjadi parameter utama dalam proses persiapan bahan uji. Jumlah tersebut adalah jumlah minimum yang berlaku dapat dianalisis. Jumlah ini harus dibawah 10 CFU diperlukan untuk menunjukkan tingkat hygienitas proses dan tingkat minimal kontaminasi. Viabilitas bakteri dan bahan tes ragi persiapan untuk tes kemahiran kecap yang diawetkan dengan L-pengeringan adalah teknik Deinococcus radiodurans (D. radiodurans) 16 tahun, 58 tahun S. cerevisiae, dan S. roxii 13 tahun. kata kunci: Viabilitas, Deinococcus, khamir, L-pengeringan, Proficiency AbstractProficiency test is one of the important activities in quality control and quality assurance microbiology laboratory for measuring the competence of analysts and analysis Proficiency test requires a model microorganism preparations are standardized quality and validity. Microbiological test of the quality of the main soy products aimed at thepresence of yeast Saccharomyces cerevisiae (S. cerevisiae), S. bailli, S. rouxii and bacterial contaminants such as Bacillus and Deinococcus. Types of yeasts and bacteria involved in the process and can be one of the important quality parameters in the preparation produced. The number and viability of bacteria and yeasts become themain parameters in the process of test preparation materials. The amount in question is the minimum number that is valid can be analyzed. This amount must be below 10 CFU required to indicate the level of hygienitas process and the minimum level of contamination. Viability of bacteria and yeast test preparation materials for proficiencytest of soy sauce that preserved by L-drying technique is Deinococcus radiodurans ( D. radiodurans ) 16 years, 58 years S. cerevisiae, and S. roxii 13 years. key words : Viability, Deinococcus, Khamir, L-drying, Proficiency


Tsitologiya ◽  
2018 ◽  
Vol 60 (7) ◽  
pp. 555-557 ◽  
Author(s):  
E. A. Alekseeva ◽  
◽  
T. A. Evstyukhina ◽  
V. T. Peshekhonov ◽  
V. G. Korolev ◽  
...  

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