scholarly journals Effect of different substrates on adaptation of arrow cane (Gynerium sagitatum Aubl.) micropropagated plants

2020 ◽  
pp. 77-84
Author(s):  
Isidro Elias Suarez Padron ◽  
José Eduardo Yépez Rodríguez ◽  
Claudia Marcela Lopez Diaz
Keyword(s):  
Plant Height ◽  
Rice Husk ◽  
Ms Medium ◽  
Test Results ◽  
Ex Vitro ◽  
River Sand ◽  
Design Data ◽  
Randomized Design ◽  
Micropropagated Plants ◽  
Separation Test

To reduce costs associated to ex vitro adaptation of arrow cane (Gynerium sagitatum Aubl.) plants Cv “Criolla”, the effect of three substrate mixes (Peat, peat + river sand and peat + rice husk) on survival, plant height and substrate associated plant cost were evaluated. Plants were micropropagated in semisolid MS medium supplied with 0,5 mg L-1 BAP. After medium removal, plants were transferred on 72-plug plastic trays filled with the respective substrate treatment. Trays were covered with translucent plastic covers during three days. Thereafter, plants were maintained in a 50% light shade house, fog irrigated twice a day for 1 minute each during 8 weeks. Treatments were distributed with a block randomized design. Data were analyzed with ANOVA and means were separated with Tukey´s mean separation test. Results allowed to evidence that peat + sand resulted in significant increase in survival, plant height and >35 decrease in substrate associated plant cost during adaptation to ex vitro conditions.

scholarly journals 883 PB 279 REGENERATING ADVENTITIOUS PLANTS FROM IN VITRO CULTURE OF LIATRIS SPICATA (L.) WILLD. COTYLEDONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560d-560
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Adventitious Shoot Formation ◽  
Murashige Skoog ◽  
Micropropagated Plants

Cotyledons from developing embryos 6 to 8 weeks old of Liatris spicata (blazing star) were cultured on Murashige-Skoog (MS) medium containing 0, 0.4, 4.4, and 44.4 μ M benzyladenine (BA) or 0, 0.2, 2.2, and 22.2 μ M thidiazuron (TDZ) to induce adventitious shoot formation. The highest percent of cotyledons forming shoots with highest shoot counts was on medium containing 2.2 μ M TDZ. Vitreous shoots formed on medium with 22.2 μ M TDZ. Callus derived from cotyledons and cultured on medium containing 4.44 μ M BA or 2.2 μ M TDZ formed adventitious shoots with highest shoot counts on 4.44 μ M BA. Adventitious shoots derived from cotyledons and callus were rooted on MS medium with 5.0 μ Mindole-3-butyric acid, acclimatized and grown ex vitro. All micropropagated plants appeared similar to each other.

scholarly journals PENGARUH PENAMBAHAN ABU SEKAM PADI (RICE HUSK ASH) DENGAN PROPORSI YANG BERBEDA TERHADAP PERTUMBUHAN SELADAI AIR (Lactuca sativa L.)

2017 ◽  
Vol 11 (2) ◽  
pp. 1-8
Author(s):  
Tri Kurniastuti ◽  
Palupi Puspitorini ◽  
Tyas Nyonita Punjungsari
Keyword(s):  
Plant Height ◽  
Rice Husk ◽  
Lactuca Sativa ◽  
Rice Husk Ash ◽  
Dry Weight ◽  
Root Dry Weight ◽  
Lactuca Sativa L ◽  
Randomized Design ◽  
Nutritional Needs ◽  
Height Increase

Lettuce vegetables (Lactuca sativa L.) much needed by the community to meet the nutritional needs, but lettuce production is still low because of low productivity. Increasing the quality and quantity of vegetables grown on polybags is by fertilizing and arranging the composition of the planting medium. Provision of fertilizer aims to increase the supply of nutrients needed plants to boost the production and quality of crops. Fertilizer is to provide or add nutrients that plants need. However, fertilizer will tend to cause high production value and less appropriate with vegetable planting at home, therefore more optimized setting the composition of planting media. Planting media should be able to provide nutrients needed by plants. Farmers use plant residual organic materials such as rice husk as and cockpit for vegetable cultivation. The rice husk is the outermost part of the grain. The rice husk ash usually contains N 0.32%, P 0.15%, K 0.31%, Ca 0.96%, Fe 180 ppm, Mn 80.4 ppm Zn 14.10 ppm and pH 6, 8 which is expected to meet the needs of plant nutrients. The study was prepared using a complete randomized design with one factor that is the addition of planting media in the form of rice husk ash with the proportion of top soil and rice husk ash as follows: M1 (3: 1), M2 (2: 2), and M3 (1: 3). The observed variables were stem height, crown dry weight, and root dry weight. Data analysis used F Test 5% and continued with Duncan Multiple Range Test (DMRT) 5%. The results obtained at plant height of M3 (1: 3) gave the best result where there was a plant height increase of 40 cm from the first day of planting until the third day, the addition of rice husk ash did not affect the addition of root dry weight and dry weight of crown.

scholarly journals PENGARUH PENAMBAHAN ABU SEKAM PADI (RICE HUSK ASH) DENGAN PROPORSI YANG BERBEDA TERHADAP PERTUMBUHAN SELADAI AIR (Lactuca sativa L.)

2017 ◽  
Vol 11 (2) ◽  
pp. 1-8
Author(s):  
Tri Kurniastuti ◽  
Palupi Puspitorini ◽  
Tyas Nyonita Punjungsari
Keyword(s):  
Plant Height ◽  
Rice Husk ◽  
Lactuca Sativa ◽  
Rice Husk Ash ◽  
Dry Weight ◽  
Root Dry Weight ◽  
Lactuca Sativa L ◽  
Randomized Design ◽  
Nutritional Needs ◽  
Height Increase

Lettuce vegetables (Lactuca sativa L.) much needed by the community to meet the nutritional needs, but lettuce production is still low because of low productivity. Increasing the quality and quantity of vegetables grown on polybags is by fertilizing and arranging the composition of the planting medium. Provision of fertilizer aims to increase the supply of nutrients needed plants to boost the production and quality of crops. Fertilizer is to provide or add nutrients that plants need. However, fertilizer will tend to cause high production value and less appropriate with vegetable planting at home, therefore more optimized setting the composition of planting media. Planting media should be able to provide nutrients needed by plants. Farmers use plant residual organic materials such as rice husk as and cockpit for vegetable cultivation. The rice husk is the outermost part of the grain. The rice husk ash usually contains N 0.32%, P 0.15%, K 0.31%, Ca 0.96%, Fe 180 ppm, Mn 80.4 ppm Zn 14.10 ppm and pH 6, 8 which is expected to meet the needs of plant nutrients. The study was prepared using a complete randomized design with one factor that is the addition of planting media in the form of rice husk ash with the proportion of top soil and rice husk ash as follows: M1 (3: 1), M2 (2: 2), and M3 (1: 3). The observed variables were stem height, crown dry weight, and root dry weight. Data analysis used F Test 5% and continued with Duncan Multiple Range Test (DMRT) 5%. The results obtained at plant height of M3 (1: 3) gave the best result where there was a plant height increase of 40 cm from the first day of planting until the third day, the addition of rice husk ash did not affect the addition of root dry weight and dry weight of crown.

scholarly journals Substitusi Media Murashige dan Skoog/MS dengan Air Kelapa dan Pupuk Daun Majemuk pada Pertumbuhan Anggrek Dendrobium secara in vitro (In Vitro Growth of Dendrobium Orchids under Substitution Murashige dan Skoog/MS Medium With Coconut Water and Compound Leaf Fertilizer)

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Annatje E.B. Inkiriwang ◽  
Jeany Mandang ◽  
Semuel Runtunuwu
Keyword(s):  
Plant Height ◽  
Coconut Water ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Compound Leaf ◽  
Randomized Design ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Leaf Fertilizer

Abstrak  Penelitian ini bertujuan  untuk  menentukan  konsentrasi  substitusi air kelapa dan pupuk   daun  majemuk  yang tepat    pada   media  MS   terhadap   pertumbuhan   anggrek Dendrobium secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 10 kali ulangan.  Variabel yang diamati adalah persentase eksplan yang bertunas, jumlah tunas, jumlah daun, tinggi tanaman dan jumlah akar. Hasil penelitian menunjukkan substitusi media MS 50 %, air kelapa 30 % dan pupuk daun majemuk (Growmore®) 1,5 g/L menghasilkan rata-rata persentase eksplan yang bertunas sebesar 6,78 %; jumlah tunas sebesar 1,26 dan tinggi tanaman sebesar 1,20 cm.Kata kunci: air kelapa, in vitro, media MS, pupuk daun majemuk  Abstract             This research was aimed to determine the proper concentration of substitution of coconut water and compound leaf fertilizer in Murashige dan Skoog (MS) medium on the in vitro growth of Dendrobium orchids. The completely randomized design experiment consisted of 7 treatments and 10 replicates. The measured parameters were the percentage of sprouted explants, number of shoots, number of leaves, plant height and number of roots. The result showed that the substitution of 50% media MS, 30% coconut water and 1.5 g/L leaf fertilizer (Growmore®) resulted the growth of shoot explant as much as 6.78% the shoot number of 1.26 and 1.20 cm plant height .Keywords: coconut water, compound leaf fertilizer, in vitro, MS medium 

scholarly journals PERTUMBUHAN BIBIT KOPI LIBERIKA TUNGKAL KOMPOSIT PADA BERBAGAI MEDIA TANAM

2019 ◽  
Vol 4 (1) ◽  
pp. 19
Author(s):  
Yulistiati Nengsih ◽  
Yuza Defitri
Keyword(s):  
Plant Height ◽  
Rice Husk ◽  
Quality Index ◽  
Peat Soil ◽  
Dry Weight ◽  
Optimal Growth ◽  
Stem Diameter ◽  
River Sand ◽  
Root Dry Weight ◽  
Total Dry Weight

Good planting media is a medium that is able to provider water and nutrients is sufficient quantities for plant growth. This can be found on soils with good air conditioning, has a solid aggregate, good water holding ability and room for sufficient rooting. Proper use of media will provide optimal growth for the plants. This study aims to determine the best planting medium to support the growth of coffe seedling Liberika Tungkal Komposit. The research was conducted in the experimental garden Pijoan, University Batanghari Jambi. From March to May 2017. The research design in this experiment was planting  medium with complete randomized environmental design (RAL). The treatment is : M0 = plant medium 100% peat soil, M1 = plant medium 100% ultisol soil, M2 = planting medium 50% untisol soil + 50% rice husk charcoal, M3 = planting medium 50% ultisol soil + 50% cocopeat, M4 = planting medium 50% ultisol soil + 50% river sand, M5 = planting medium 50% peat soil + 50% rice husk charcoal, M6 = planting medium 50% peat soil + 50% cocopeat, M7 = planting medium 50% peat soil + 50% river sand. Parameter observed were plant height, stem diameter, root length, crown dry weight, root dry weight, root canopy ratio, total dry weight, quality index, initial pH media and final pH media. The result showed that the ultisol 100% ultisol garden media gave the heighes seed growth value based on single parameter ie plant height, stem diameter, root lenght, crown dry weight, root dry weight, root canopy ratio, total dry weight, and initial media pH and treatment integrated quality index that indicates that the seed is most ready to be moved to the field.Keywords: nurseries, plantations, coffee AbstrakMedia tanam yang baik adalah media yang mampu menyediakan air dan unsur hara dalam jumlah cukup bagi pertumbuhan tanaman. Hal ini dapat ditemukan pada tanah dengan tata udara yang baik, mempunyai agregat mantap, kemampuan menahan air yang baik dan ruang untuk perakaran yang cukup.Penggunaan media yang tepat akan memberikan pertumbuhan yang optimal bagi tanaman. Percobaan bertujuan untuk mengetahui media tanam yang paling baik untuk menunjang pertumbuhan bibit kopi Liberika Tungkal Komposit. Penelitian dilakukan di Kebun Percobaan Fakultas Pertanian Universitas Batanghari di Pijoan, Muaro Jambi. Percobaan dilaksanakan pada bulan Maret sampai Mei 2017. Percobaan menggunakan rancangan lingkungan acak lengkap dan rancangan perlakuan media tanam (M) sebagai berikut: M0 = 100% gambut, M1= 100% tanah jenis ultisol, M2= 50% tanah jenis ultisol + 50% arang sekam padi, M3= 50% tanah jenis ultisol + 50% cocopeat, M4 = 50% tanah jenis ultisol + 50% pasir sungai, M5= 50% tanah gambut + 50% arang sekam padi, M6= 50% tanah gambut + 50% cocopeat, M7= 50% tanah gambut + 50% pasir sungai. Parameter yang diamati adalah pH awal dan pH akhir media, tinggi tanaman, diameter batang, panjang akar, bobot kering tajuk, bobot kering akar, nisbah tajuk akar, bobot kering total dan indek kualitas. Hasil percobaan menunjukkan media tanam 100% tanah jenis ultisol  menghasilkan pertumbuhan bibit tertinggi berdasarkan parameter tunggal yaitu tinggi tanaman, diameter batang, panjang akar, bobot kering tajuk, bobot kering akar, nisbah tajuk akar, bobot kering total dan parameter terintegrasi yaitu indeks kualitas.Kata kunci:  pembibitan, perkebunan, kopi

scholarly journals Effect of Variegated Forest Soil Amendments on the Germination and Early Growth of Irvingia gabonensis (O Rorke, Baill)

2020 ◽  
pp. 43-50
Author(s):  
A. E. Egwunatum ◽  
D. E. Dolor ◽  
C. J. Ofobike
Keyword(s):  
Growth Period ◽  
Early Growth ◽  
River Sand ◽  
Design Data ◽  
Randomized Design ◽  
Soil Media ◽  
Irvingia Gabonensis ◽  
Collar Diameter ◽  
Germination And Growth ◽  
Range Test

This study evaluated the early germination and growth variables of Irvingia gabonensis under organically primed and thermally amended soil media. Six media were prepared from Gmelina plantation topsoil by amendment with poultry waste (T2), river sand (T3) and combusted forest floor litters for 5 (T4), 10 (T5), 15 (T6) and 20 (T7) minutes respectively to contrast a control topsoil (T1). Soil media were analyzed for critical nutrient properties and engaged in the germination and early growth trial of Irvingia replicated three times, and arranged in a completely randomized design. Data collected were analyzed with ANOVA and significant means separated with the Duncan multiple range test. The results showed that Ca/Mg ratio was widest for T3, least CEC (13.2 meq/100 g soil) by T7 and slightly acidic pH (H2O) for T1 and T2 while T3, T4, T6 and T7 were alkaline. Germination at 6WAS was T4 (100%)> T1=T2=T5 (85.71%) > T3 (71.43%) > T6 (42.86%) > T7 (0%). The growth variables of seedling varied significantly (P<0.05) with T3 and T4 comparing favorably in collar diameter (4.50x10-1± 0.05 mm) and leaf area (35.08±4.85 mm2) although T3 recorded the highest stem height (117.79±0.42 mm). The study recommends the use of least thermally modified media (T4) and primed topsoil-riversand (T3) for fast germination in view of conserving food reserve for the critical early growth period of I. gabonensis in pursuit of its domestication in nutrient degraded soils.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

Physical Properties of Cement Mortar Containing Waste Ash

2015 ◽  
Vol 804 ◽  
pp. 129-132
Author(s):  
Sumrerng Rukzon ◽  
Prinya Chindaprasirt
Keyword(s):  
Compressive Strength ◽  
Fly Ash ◽  
Rice Husk ◽  
Rice Husk Ash ◽  
Cement Mortar ◽  
Ash Content ◽  
Water Requirement ◽  
Test Results ◽  
Pozzolanic Material ◽  
Materials Used

This research studies the potential for using waste ash from industrial and agricultural by-products as a pozzolanic material. Classified fly ash (FA) and ground rice husk ash (RA) were the materials used. Water requirement, compressive strength and porosity of cement mortar were investigated. Test results indicated that FA and RA (waste ash) have a high potential to be used as a good pozzolanic material. The water requirement of mortar mix decreases with the increases in fly ash content. For ground rice husk ash (RA), the water requirement of mortar mix increases with the increases in rice husk ash content. In addition, the reduction in porosity was associated with the increase in compressive strength.

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

DIFFERENTIAL EFFECT OF SALINITY ON GROWTH, NUTRIENT CONCENTRATION AND CHLOROPHYLL IN SUGARCANE VARIETIES MEX 69-290 AND CP 72-2086

Agrociencia ◽  
2021 ◽  
Vol 55 (7) ◽  
pp. 597-610
Author(s):  
Odón Castañeda Castro ◽  
Miriam Cristina Pastelín Solano ◽  
Libia Iris Trejo Téllez ◽  
Eduardo Ariel Solano Pastelín ◽  
Fernando Carlos Gómez Merino
Keyword(s):  
Plant Height ◽  
Plant Performance ◽  
Saccharum Spp ◽  
Sugarcane Varieties ◽  
Randomized Design ◽  
Affected Plant ◽  
Nacl Concentration ◽  
Irrigation Solution ◽  
Completely Randomized Design ◽  
Better Than

Sugarcane (Saccharum spp. hybrids) is moderately sensitive to salinity and the effects on plant performance vary according to stress level and genotype. This study aimed to evaluate the effect of salt stress induced by application of different NaCl levels in the irrigation solution on plant height, indirect index of chlorophylls (SPAD), and macronutrients concentration (N, P, K, Ca, Mg and S) and Na in leaves of two sugarcane varieties: CP 72-2086 and Mex 69-290. The experiment was set in a completely randomized design with a 2×5 factorial arrangement. The study factors were sugarcane variety (CP 72-2086 and Mex 69-290) and NaCl concentration (0.0, 71.8, 143.6, 215.4 and  282.7 mM NaCl). Salinity as a single factor negatively affected plant height, SPAD units and N and P concentration in leaves; Ca concentrations increased, while K, Mg and S remained unaffected by the tested NaCl levels. Mex 69-290 grew higher and concentrated greater levels of N and K. Interactions of factors showed that salinity reduced growth in both varieties, but this reduction was more pronounced in CP 72-2086. SPAD units were also significantly reduced by salinity in both varieties. Concentrations of N and P in leaves decreased in both varieties in response to NaCl, while those of K and Ca increased in Mex 69-290. Concentration of Na was higher in Mex 69-290 which exhibited better performance than CP 72-2086. Sodium concentrations in leaves increased in direct relation to the tested NaCl concentrations. Mex 69-290 reached higher concentrations of Na in leave tissues but displayed better health than CP 72-2086. Thus, the variety Mex 69-290 showed more efficient Na-tolerance mechanisms related to Ca and K concentrations, and an indirect chlorophyll index better than CP 72-2086.

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