Manejo integrado de chisas en fincas de minifundio del departamento de Nariño (Colombia)

La ‘chiza’ o ‘cuzo’ (Astaena sp.) constituye un problema insectil de importancia económica en el departamento de Nariño, especialmente en los suelos que han sufrido procesos de degradación con pérdida de materia orgánica. En los municipios de Ospina y Yacuanquer las chisas ocasionan daños de 91% y 62% en cultivos de trigo y papa, respectivamente. Para su control, los agricultores utilizan productos químicos como única alternativa, lo que ha generado impactos negativos: incremento de los costos de producción, reducción de los rendimientos agrícolas, necesidad de mayor cantidad de semilla y fertilizante, pérdida del valor comercial de los predios y disminución de la fauna benéfica que controlaban las chisas en niveles inocuos a los cultivos. En los ensayos de campo se aplicaron microorganismos entomopatógenos nativos (Beauveria bassiana, Metarhizium anisopliae y Steinernema sp.), utilizando tamo de trigo descompuesto para su establecimiento. Con la aplicación de los hongos se logró un porcentaje de mortalidad de la plaga de 28,75% en el municipio de Ospina y de 14,67% en el municipio de Yacuanquer, frente a la aplicación de clorpirifos cuyo porcentaje de mortalidad fue de 28,89% en el municipio de Ospina y 18,82% en el municipio de Yacuanquer. Con el nematodo (Steinernema sp.) se alcanzó una mortalidad de 17,27% en Ospina y 12,74% en Yacuanquer. La aplicación de una mezcla de microorganismos entomopatógenos y tamo de trigo descompuesto a los cultivos contribuye a disminuir la contaminación ambiental y a mejorar las características de los suelos. Integrated management of white grubs on small farms in the Nariño department (Colombia) White grubs (Astaena sp.) represent an economically important insect pest in the Nariño department, especially on farms which have become degraded due to the loss of organic matter. White grubs have caused up to 91% and 62% damage to total wheat and potato crop areas, respectively, in the municipalities of Ospina and Yacuanquer. To controlling the pest, farmers use only chemical pesticides which has led to negative impacts, such as increased production costs, reduced yields, need for greater amounts of seeds and fertilisers, farms losing their commercial value and a reduction in beneficial fauna which formerly kept white grubs at levels causing no damage to the crops over a long period of time. The native entomopathogen microorganisms Beauveria bassiana, Metarhizium anisopliae and Steinernema sp. were established in rotting wheat husks in field tests. The entomopathogenic fungi led to 28.75% and 14.67% of pest mortality at Ospina and Yacuanquer localities respectively compared to 28.89% and 18.82% when applying the chemical insecticide (Clorpirifos). The nematode Steinernema sp. caused 17.26% and 12.74% of white grub mortality. This technology (mixing entomopathogens micro-organisms with rotting wheat husks) contributed towards reducing environmental human contamination and improving soil characteristics.

Download Full-text

Evaluación de la actividad biocontroladora de Beauveria bassiana y Metarhizium anisopliae sobre larvas de Ancognatha scarabaeiodes (Coleoptera: Scarabaeidae)

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol5_num1_art:23 ◽

2004 ◽

Vol 5 (1) ◽

pp. 43

Author(s):

Any Mercedes Lucero Mafla ◽

Luis Alberto Peña Villamil ◽

Tito Bacca Ibarra

Keyword(s):

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Crop Yields ◽

Integrated Management ◽

White Grubs ◽

Promising Alternative ◽

Fungal Entomopathogens ◽

Biocontrol Activity ◽

Mortality Percentage ◽

Lethal Doses

Actualmente, en los municipios del departamento de Nariño (Colombia), en los cultivos de trigo y papa que son los principales sistemas de producción, se presentan altas infestaciones de poblaciones de chisas encontrándose hasta 350 larvas/m2, representando un factor limitante para la producción de los cultivos. Una alternativa promisoria para el manejo de esta plaga es la utilización de métodos biológicos que incluyen el uso de hongos entomopatógenos. El objetivo del presente trabajo fue el de evaluar el efecto de Beauveria bassiana, Metarhizium anisopliae sobre larvas de Ancognatha scarabaeoides en laboratorio e invernadero. Los aislamientos de hongos se obtuvieron de larvas infectadas en los municipios de Yacuanquer y Ospina. En el laboratorio, se seleccionaron 5 de 9 aislamientos de hongos: Mt1, Bb cosmo, Mt2, Bb4 y Bb10 que presentaron mortalidades superiores al 50%, éstas se evaluaron a concentraciones de 1x105 a 1x1010 esporas/ml. El porcentaje acumulado de mortalidad a los 18 días después de la inoculación fueron: del 100% para Bb cosmo, Mt1 y Mt2 en la concentración de 1x1010 esporas/ml y del 90% para Bb4 y Bb10 en la concentración de 1x109 esporas/ml, presentando concentraciones letales 90(CL90) de 1.1x1010, 7.3x109, 2.2x1010, 3.9x1010 y 4.3x1010 esporas/ml respectivamente. En invernadero se evaluaron los mismos microorganismos con sus respectivas concentraciones letales 50(CL50) y 90(CL90), encontrándose porcentajes de mortalidad del 76.00%, 67.50%, 63.50%, 59.00% y 53.50% para Mt1, Bb cosmo, Mt2, Bb4 y Bb10. Estos resultados indican la posibilidad de incluir estos biocontroladores nativos en el manejo integrado de las chisas en Nariño. Evaluation of the biocontrol activity of Beauveria bassiana, Metarhizium anisopliae, on larvae of Ancognatha scarabaeoides (Coleoptera: Scarabaeidae).High grub populations of coleopteran, frequently attack crops of wheat and potato in the Department of Nariño, Colombia. Reports indicate that infestations levels reach more than 350 larvae/ m2, which severely reduce crop yields. Use of biological control measurements as fungal entomopathogens seems to be a promising alternative for management of this pest. The present work aimed to evaluate the effect of Beauveria bassiana and Metarhizium anisopliae on larvae of Ancognatha scarabaeoides both in laboratory and greenhouse conditions. Isolates of entomopathogen fungus were obtained from infected larvae in the municipalities of Yacuanquer and Ospina. A sample of 5 isolates, which previously had shown to cause mortality rates higher than 50%, was selected in the laboratory: Mt1, Bb cosmo, Mt2, Bb4 and Bb10. Isolates concentrations from 1x105 to 1x1010 spores/ml were tested. The cumulative mortality percentage (at 18 days after the inoculation) ranged from 100% with Bb cosmo, Mt1 and Mt2 at concentrations of 1x1010 spores/ml to 90% with Bb4 and Bb10 at concentrations of 1x109 spores/ml. Lethal doses 90 (LD90) of 1.1x1010, 7.3x109, 2.2x1010, 3.9x1010 and 4.3x1010 spores/ml were calculated for each isolate, respectively. An evaluation of the biocontrol activity of the isolates was carried out under greenhouse conditions. Mortality percentages obtained reached 76%, 67.5%, 63.5%, 59% and 53.5% for Mt1, Bb cosmo, Mt2, Bb4 and Bb10, respectively. These results suggest that the use of native biocontrolers is a promising alternative to be included in the integrated management for white grubs in Nariño.

Download Full-text

Pengaruh Pupuk Kandang dan Insektisida Kimia Terhadap Efektivitas Jamur Metarhizium anisopliae pada Uret Tebu, Lepidiota stigma

Buletin Tanaman Tembakau Serat & Minyak Industri ◽

10.21082/btsm.v11n1.2019.33-45 ◽

2019 ◽

Vol 11 (1) ◽

pp. 33

Author(s):

I Gusti Agung Ayu Indrayani ◽

Kristiana Sri Wijayanti ◽

Heri Prabowo

Keyword(s):

Laboratory Study ◽

Sugar Cane ◽

Metarhizium Anisopliae ◽

Block Design ◽

Insect Pest ◽

Animal Manure ◽

Control Technique ◽

White Grub ◽

Chemical Insecticide ◽

Screen House

Uret tebu Lepidiota stigma (Coleoptera: Scarabaeidae) adalah salah satu hama penting pada tanaman tebu yang pada tingkat serangan parah menyebabkan penurunan produksi tebu. Pengendalian hama uret ini dengan menggunakan jamur M. anisopliae menawarkan suatu teknik pengendalian yang biologis, efektif, dan aman bagi lingkungan. Penelitian ini dilakukan di Laboratorum Patologi Serangga dan di rumah kasa Balai Penelitian Tanaman Pemanis dan Serat (Balittas) Malang dengan tujuan untuk mengevaluasi efektifitas jamur M. anisopliae terhadap uret tebu, L. stigma. Penelitian terdiri atas pengujian di laboratorium dan di rumah kasa. Perlakuan yang diuji di laboratorium adalah: (1) M. anisopliae (MA), (2) M. anisopliae + imidakloprid 5% (1 mg/vial) imidakloprid 1 mg/vial (MA + K-1), (3) M. anisopliae + pupuk kandang (MA + P), (4) M. anisopliae + imidakloprid 5% (2 mg/vial), imidakloprid 2 mg/vial (MA + K-2), (5) Metastigma (produk bioinsektisida berbasis jamur M. anisopliae sebagai pembanding) , dan (6) Kontrol (tanpa perlakuan). Perlakuan yang sama juga diujikan di rumah kasa. Perlakuan di laboratorium disusun dalam Rancangan Acak Lengkap (RAL), dengan empat ulangan dan perlakuan di rumah kasa disusun dengan Rancangan Acak Kelompok (RAK), diulang empat kali. Parameter yang diamati di laboratorium dan di rumah kasa adalah mortalitas uret L. stigma. Hasil penelitian di laboratorium menujukkan bahwa penambahan pupuk kandang pada jamur M. anisopliae efektif meningkatkan mortalitas uret sebesar 12,9%, sedangkan di rumah kasa penambahan pupuk kandang maupun insektisida kimia imidakloprid pada jamur M. anisopliae tidak efektif meningkatkan mortalitas uret L. stigma.<div> </div><div>Influence of Animal Manure and Chemical Insecticide on Effectivity of Metarhizium anisopliae against Sugarcane White grub, Lepidiota stigma</div>White grub Lepidiota stigma (Coleoptera: Scarabaeidae) is one of important insect pest on sugar cane which is on highest level of attack causes decline production of sugar cane. Control this pest by using entomopathogenic fungi M. anisopliae offer a biological control technique that is effective and environmentally friendly. This study conducted in laboratory and in screen house with aimed to evaluate the influence of animal manure and chemical insecticide imidakloprid on the effectivity of M. anisopliae against sugarcane white grub, L. stigma. In the laboratory study, treatments tested were (1) M. anisopliae, (2) M. anisopliae + imidacloprid 5% (1 mg/vial), (3) M. anisopliae + manure, (4) M. anisopliae + imidacloprid 5% (2 mg/vial), (5) Metastigma (comparison treatment), and (6) Untreated control. The equal treatments were also tested in screen house study. Treatments in the laboratory study were arranged in Completely Randomized Design (CRD) with four replications, while the treatments in screen house were arranged in Randomized Block Design (RBD) with four replicates. Parameters observed in both laboratory and screen house studies were mortality of whitegrub, L. stigma. Results showed that in the laboratory, addition of animal manure on M. anisopliae application increased 12.9% of white grub mortality, however, at screen house study on addition of animal manure or imidacloprid 5% on M. anisopliae application was less effective in order to enhance the mortality of white grub.

Download Full-text

On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00428-z ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Muhammad Shehzad ◽

Muhammad Tariq ◽

Tariq Mukhtar ◽

Asim Gulzar

Keyword(s):

Sex Ratio ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Plutella Xylostella ◽

Metarhizium Anisopliae ◽

Insect Pest ◽

Diamondback Moth ◽

Economic Damage ◽

Offspring Sex Ratio ◽

Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

Download Full-text

Biological control of white grubs, Holotrichia serrata (Fabricius) in sugarcane by two species of entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana

International Journal of Tropical Insect Science ◽

10.1007/s42690-020-00256-y ◽

2020 ◽

Author(s):

B. Ramanujam ◽

A.C. Hosamani ◽

B. Poornesha ◽

E. Sowmya

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

White Grubs ◽

Holotrichia Serrata

Download Full-text

UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.11352-60 ◽

2013 ◽

Vol 13 (1) ◽

pp. 52-60

Author(s):

Haperidah Nunilahwati ◽

Siti Herlinda ◽

Chandra Irsan ◽

Yulia Pujiastuti ◽

Khodijah Khodijah ◽

...

Keyword(s):

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Active Ingredient ◽

Plutella Xylostella ◽

Metarhizium Anisopliae ◽

Entomopathogenic Fungus ◽

Crop Production ◽

Insect Pest ◽

Larval Mortality ◽

Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory. The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

Download Full-text

Pathogenicity of Metarhizium anisopliae and Beauveria bassiana against Phyllophaga crinita and Anomala flavipennis (Coleoptera: Scarabaeidae)

Journal of Entomological Science ◽

10.18474/0749-8004-40.1.67 ◽

2005 ◽

Vol 40 (1) ◽

pp. 67-73 ◽

Cited By ~ 5

Author(s):

L. A. Rodríguez-del-Bosque ◽

F. Silvestre ◽

V. M. Hernández ◽

H. Quiroz ◽

J. E. Throne

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Biological Control Agent ◽

Challenge Test ◽

Control Agent ◽

The Other ◽

White Grub ◽

Test Protocol ◽

Lethal Time

Five isolates of Beauveria bassiana (Balsamo) Vuillemin and 3 isolates of Metarhizium anisopliae (Metschnikoff) Sorokin were tested against third-instar larvae of Phyllophaga crinita (Burmeister) and Anomala flavipennis Burmeister under laboratory conditions using the “maximum challenge test” protocol. The M. anisopliae strains were more virulent than the B. bassiana strains, with the isolates MAGL3N and MAGL4N of M. anisopliae causing the highest mortality in both white grub species. Regardless of scarab species, mortality caused by MAGL3N was >63% after 4 days and >96% after 10 days. The other strain of M. anisopliae, MAGC2N, also caused high mortality in A. flavipennis, but at a slower rate than MAGL3N. Median lethal time (LT50) for MAGL3N was 2.9 days for P. crinita and 3.0 d for A. flavipennis. The LT50 for MAGL4N was 5.3 d for P. crinita and 7.6 d for A. flavipennis while the LT50 for MAGC2N was 4.4 d for A. flavipennis. Metarhizium anisopliae is a potential biological control agent for P. crinita and A. flavipennis and should be further investigated for possible development.

Download Full-text

Activity of strains of Metarhizium anisopliae (Metsch) Sorokin against white grub (Chiloloba acuta, Scarabaeidae, Coleoptera) under laboratory condition in Nepal

Journal of Agriculture and Environment ◽

10.3126/aej.v17i0.19864 ◽

2018 ◽

Vol 17 ◽

pp. 82-91 ◽

Cited By ~ 1

Author(s):

Dipak Khanal

Keyword(s):

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Insect Pests ◽

Microbial Control ◽

White Grub ◽

White Grubs ◽

Larval Stages ◽

Commercial Strain ◽

Randomized Design ◽

Completely Randomized Design

Soil insect pests are the major productivity constrains of different crops among which white grubs (Coleoptera: Scarabaeidae), both adult and larval stages, are extremely destructive in nature. Laboratory studies were conducted to evaluate the virulence of an indigenous and a commercial strain of the entomopathogenic fungi, Metarhizium anisopliae (Metsch.) Sorokin, against white grubs species Chiloloba acuta by applying the dipping method at Entomology Division, NARC, Nepal. Third instars larvae of C. acuta were dipped in suspensions of indigenous and commercial strains (Pacer) of M. anisopliae at different concentrations ranging from 3.33×104 to 1.04×108 spores/ml for 3-5 seconds which resulted in 97.8% and 89% mortalities with the highest dose of 1.04×108 spores/ml 40 days after inoculation (DAI), respectively. The LC50 values for indigenous and commercial strain were 3.5×105 and 1.88×106 spores/ml, respectively, with the potency of 1:0.28 at 40 DAI. Bioassays were conducted in completely randomized design. From results it is concluded that the tested strains of entomopathogenic fungi have potential as microbial control agents in managing white grubs in laboratory and it is suggested to be tested under farmers field condition.

Download Full-text

LABORATORY EVALUATION OF BENGKULU ISOLATES OF Beauveria bassiana AND Metarhizium anisopliae AGAINST COFFEE BERRY BORER, Hyphotenemus hampei, USING SPRAYING METHOD

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.21993-100 ◽

2019 ◽

Vol 19 (2) ◽

pp. 93

Author(s):

Dwinardi Apriyanto ◽

Nadrawati .

Keyword(s):

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Insect Pests ◽

Insect Pest ◽

Laboratory Evaluation ◽

Stink Bug ◽

Coffee Berry ◽

Coffee Berry Borer ◽

Pure Cultures ◽

June July

Laboratory Evaluation of Local Isolates of Beauveria bassiana and Metarhizium anisopliae against Coffee Berry Borer, Hyphotenemus hampei, using spryaing method. Beauveria bassiana Bals (Vuillemin) and Metarhizium anisopliae (Metschnikoff) Sorokin are two species of fungi that have been studied widely and used as biological control of various insect pests, including coffee berry borer (CBB), Hyphotenemus hampei Ferrari. Laboratory studies were done in June – July 2017, to evaluate local isolates of both fungi species against CBB, the most destructive insect pest of coffee worldwide, including in Indonesia. B. bassiana was isolated from dead sweet potato weevil (Cylas formicarius) and green stink bug (Nezara viridula), and from soil. M. anisopliae was isolated from soil. The pure cultures of the fungi were mass-cultured on corn based media. The conidia harvested from 4 week colonies was hand sprayed directly upon CBB adults (females) and damaged coffee berry at the concentration of 109 ml-1. In additions, the conidia of B. bassiana isolated from C. formicarius alone was diluted to 106- 109 ml-1 and applied to damaged coffee berry. The results showed that when the conidia were applied directly upon the insects, the mortality of beetle were higher in B. bassiana than in M. anisopliae treatments. Pooled data indicated that B. bassiana isolated from death insects significantly caused higher mortality than did M. anisopliae isolated from soil. Beetle mortality was 76.7 and 80.0% for B. basiana, and 60.3 and 60% for M. anysopliae treatments. Application upon damage coffee berry indicated much lower mortality. Application of higher concentration upon damaged coffee berry resulted higher mortality, but data are not consistent. The mortality of CBB larvae was much less and negligible even at the highest conidia concentration.

Download Full-text

Area wide management of white grub Holotrichia consanguinea Blachard through IPM modules in groundnut

The Journal of Phytopharmacology ◽

10.31254/phyto.2021.10313 ◽

2021 ◽

Vol 10 (3) ◽

pp. 215-219

Author(s):

Bhut JB ◽

◽

Jethva DM ◽

Naggana R ◽

◽

...

Keyword(s):

Beauveria Bassiana ◽

Aggregation Pheromone ◽

Metarhizium Anisopliae ◽

Seed Treatment ◽

Field Experiments ◽

Plant Mortality ◽

White Grub ◽

Yield Increase ◽

Castor Cake ◽

Block Tree

Field experiments were conducted on groundnut for white grub Holotrichia consanguinea Blachard management using different IPM components in Gujarat during kharif 2018 and 2019. Two different eco-friendly modules were evaluated and compare with farmers practices. In Module I included on set of monsoon, spraying of chlorpyrifos 20 EC @ 0.04% on surrounding trees, seed treatment of chlorpyrifos 20 EC @ 20 ml/kg, Placement of aggregation pheromone on host tree (5 block/tree), Soil application of Beauveria bassiana @ 5 kg ha-1 (Min. 2 x 106 CFU/g) + castor cake (250 kg ha-1) before sowing, Application of B. bassiana @ 5 kg ha-1 in plant row with FYM (250 kg ha-1) after 30 days of germination. While Module II used Metarhizium anisopliae instead of B. bassiana. While in farmers practices included application of chlorpyriphos 10 G @ 10 kg ha-1 with urea at initiation of pest attack. Among the evaluated two ecofriendly module and farmers practices the lowest per cent plant mortality (3.03 %) and 0.56 grub/m2 was recorded in Module II. The highest pod and halum yield 2180 and 3942 kg ha-1, yield increase over control 31.00 and 39.37 %, avoidable loss 23.67 and 28.25% was recorded in module II. The maximum (32474 Rs/ha) net realization was found in the treatment of Module II. Looking to the NICBR, the highest (1:5.35) return was obtained with the treatment of Module II followed by Module I (1:2.93)

Download Full-text

Evaluación de hongos entomopatógenos para el control biológico del mosquito Culex quinquefasciatus

Revista Clepsidra ◽

10.26564/19001355.198 ◽

2009 ◽

Vol 5 (9) ◽

pp. 25-38

Author(s):

Martha Giselle Rivera Pineda ◽

Sandra Patricia Garzón Lozano ◽

Luz Inés Villarreal Salazar

Keyword(s):

Beauveria Bassiana ◽

Culex Quinquefasciatus ◽

Metarhizium Anisopliae

En busca de una alternativa de control del mosquito Culex quinquefasciatus en áreas urbanas se desarrolló el proyecto: “Evaluación de microorganismos biocontroladores sobre el mosquito Culex quinquefasciatus” cuyo objetivo fue evaluar la capacidad biocontroladora de diferentes hongos entomopatógenos sobre larvas del mosquito a nivel de laboratorio. Se evaluaron 16 aislamientos de hongos en diferentes concentraciones. Se constataron diferencias en el porcentaje de mortalidad producido en larvas de Cx. quinquefasciatus dependientes del tipo de aislamiento, de la concentración utilizada y del tiempo de exposición. Los aislamientos que presentaron el nivel de mortalidad más alto pertenecen a la especie Metarhizium anisopliae, seguidos por aislamientos de la especie Beauveria bassiana. El aislamiento que presentó el mayor nivel de mortalidad (100%) en menor tiempo de exposición (24 horas) fue Metarhizium anisopliae 99068 en una concentración de 1x106 e/ml. Por lo tanto este aislamiento es recomendado para su evaluación en campo y desarrollo de un producto comercial.

Download Full-text