scholarly journals Micropropagación in vitro de naranja agria (Citrus aurantium L.) a partir de segmentos nodales

Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2216-2221
Author(s):  
Angel David Hernández-Amasifuen ◽  
Alexandra Jherina Pineda-Lázaro ◽  
Hermila Belba Díaz-Pillasca
Keyword(s):  
Citrus Aurantium

La naranja agria (Citrus aurantium L.) presenta un alto valor nutricional y gastronómico en el distrito de Huacho, Lima, Perú, pero en la actualidad se considera una especie en peligro de desaparecer del distrito y alrededores por problemas fitosanitarios. Para la recuperación y repoblación de esta especie se planteó emplear técnicas biotecnológicas para la obtención de plantas libres de patógenos. Por lo tanto, el objetivo de la presente investigación fue micropropagar in vitro naranja agria a partir de segmentos nodales. Los segmentos nodales de naranja agria fueron desinfectados en diferentes concentraciones de NaClO, luego se introdijeron en medio de cultivo MS (Murashige y Skoog) adicionado con BAP, KIN y AG3 para la fase de multiplicación, posteriormente se transfirieron a medios MS adicionado con IBA y ANA para la fase de enraizamiento. La evaluación del porcentaje de contaminación se realizó a los diez días, la evaluación de formación de brotes en fase de multiplicación se realizó a los 30 días y la evaluación de enraizamiento a los 30 días. En la fase de desinfección y establecimiento in vitro se logró obtener 0% de contaminación y 0% de oxidación de los explantes. En la fase de multiplicación in vitro los mejores resultados se obtuvieron en el medio de cultivo M8 generando 4,7 brotes por explante. Y finalmente en la fase de enraizamiento el medio E4 permitió obtener 94,7% de explantes enraizados, 23,4 mm de longitud de raíz y 2,2 raíces por explante.

scholarly journals Phytochemical Profile, α-Glucosidase, and α-Amylase Inhibition Potential and Toxicity Evaluation of Extracts from Citrus aurantium (L) Peel, a Valuable By-Product from Northeastern Morocco

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1555
Author(s):  
Ouijdane Benayad ◽  
Mohamed Bouhrim ◽  
Salima Tiji ◽  
Loubna Kharchoufa ◽  
Mohamed Addi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Lower Amount ◽  
Water Mixture ◽  
Citrus Aurantium ◽  
Bioactive Substances ◽  
Citrus Peel

Due to the high volume of peel produced, Citrus by-product processing could be a significant source of phenolic compounds, in addition to essential oil. Citrus fruit residues, which are usually dumped as waste in the environment, could be used as a source of nutraceuticals. Citrus aurantium (L), also known as sour or bitter orange, is a member of the Rutaceae family and is the result of interspecific hybridization between Citrus reticulata and Citrus maxima. The purpose of this study is to chemically and biologically evaluate the peel of C. aurantium, which is considered a solid waste destined for abandonment. To achieve more complete extraction of the phytochemicals, we used a sequential extraction process with Soxhlet using the increasing polarity of solvents (i.e., cyclohexane, chloroform, ethyl acetate, acetone, and ethanol–water mixture). Essential oil (EO) from the Citrus peel, which was present at 1.12%, was also prepared by hydrodistillation for comparison. Various phytochemical assays were used to determine the qualitative chemical composition, which was subsequently characterized using GC-MS and HPLC-DAD. The inhibitory effects of C. aurantium peel extract on two enzymes, intestinal α-glucosidase and pancreatic α-amylase, were measured in vitro to determine their potential hypoglycemic and antidiabetic actions. Each extract had a significantly different phytochemical composition. According to GC-MS analyses, which allow the identification of 19 compounds, d-limonene is the most abundant compound in both EO and cyclohexane extract, at 35.17% and 36.15 % (w/w). This comparison with hydrodistillation shows the value of the sequential process in extracting this valuable terpene in large quantities while also allowing for the subsequent extraction of other bioactive substances. On the contrary, linoleic acid is abundant (54.35% (w/w)) in ethyl acetate extract (EAE) with a lower amount of d-limonene. HPLC-DAD analysis allows the identification of 11 phytochemicals, with naringenin being the most abundant flavanone, detected in acetone extract (ACE) (23.94% (w/w)), ethanol–water extract mixture (EWE) (28.71% (w/w)), and chloroform extract (CFE) (30.20% (w/w)). Several extracts significantly inhibited α-amylase and/or α-glycosidase in vitro. At a dose of 332 g/mL, ACE, CFE, and EWE inhibited the two enzymes by approximately 98%. There were strong significant correlations between naringenin and α-glucosidase inhibition and between gallic acid and α-amylase inhibition. Molecular docking experiments further verified this. Finally, oral administration of C. aurantium extracts at a dose of 2000 mg/kg did not cause any effect on mice mortality or signs of acute toxicity, indicating that it is non-toxic at these doses. These findings suggest that C. aurantium peels could be a valuable by-product by providing a rich source of non-toxic phytoconstituents, particularly those with potential antidiabetic action that needs to be confirmed in vivo.

scholarly journals Bitter Orange (Citrus aurantium Linné) Improves Obesity by Regulating Adipogenesis and Thermogenesis through AMPK Activation

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1988 ◽  
Author(s):  
Park ◽  
Kim ◽  
Jung ◽  
Ahn ◽  
Kwak ◽  
...  
Keyword(s):  
Uncoupling Protein ◽  
Underlying Mechanism ◽  
Peroxisome Proliferator ◽  
Citrus Aurantium ◽  
Brown Adipocytes ◽  
Peroxisome Proliferator Activated Receptor ◽  
Bitter Orange ◽  
Enhancer Binding Protein ◽  
Amp Activated Protein Kinase

Obesity is a global health threat. Herein, we evaluated the underlying mechanism of anti-obese features of bitter orange (Citrus aurantium Linné, CA). Eight-week-administration of CA in high fat diet-induced obese C57BL/6 mice resulted in a significant decrease of body weight, adipose tissue weight and serum cholesterol. In further in vitro studies, we observed decreased lipid droplets in CA-treated 3T3-L1 adipocytes. Suppressed peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer binding protein alpha indicated CA-inhibited adipogenesis. Moreover, CA-treated primary cultured brown adipocytes displayed increased differentiation associated with elevation of thermogenic factors including uncoupling protein 1 and PPARγ coactivator 1 alpha as well. The effects of CA in both adipocytes were abolished in AMP-activated protein kinase alpha (AMPKα)-suppressed environments, suggesting the anti-adipogenic and pro-thermogenic actions of CA were dependent on AMPKα pathway. In conclusion, our results suggest CA as a potential anti-obese agent which regulates adipogenesis and thermogenesis via AMPKα.

scholarly journals Bioactividad de extracto de Annona muricata y aceites esenciales de Citrus aurantium contra Phytophthora cinnamomi

2016 ◽  
Vol 8 ◽  
pp. 325 ◽  
Author(s):  
DEIMER VITOLA R ◽  
ALEXANDER PÉREZ C
Keyword(s):  
Phytophthora Cinnamomi ◽  
Citrus Aurantium ◽  
Annona Muricata ◽  
Para Determinar

El objetivo de esta investigación fue determinar la actividad antifúngica in vitro contra P. cinnamomi de aceites esenciales de hojas frescas de naranja agria recolectada en los municipios de Sincelejo, Sampués y La Unión, y de extractos en diclorometano de hojas secas de guanábana recolectadas en los municipios de Sincelejo y La Unión, departamento de Sucre. Se utilizó el método de siembra directa para determinar la bioactividad de los aceites y extractos. Para los aceites esenciales se prepararon concentraciones de 15 µL/mL, 17,5 µL/mL y 20 µL/mL y para los extractos vegetales se prepararon concentraciones de 0,05 g/mL, 0,07 g/mL, 0,1 g/mL y 0,5 g/mL. Encontrándose que para todos los aceites esenciales y extractos la concentración que mostró resultados similares al control positivo (Agrifos) fue de 20 µL/mL y 0,5 g/mL respectivamente. Con respecto a los perfiles cromatográfico los aceites esenciales de Sampués y La Unión mostraron como metabolito mayoritario al Linalool, mientras que el recolectado en Sincelejo mostró al Antranilato de linalilo. Para los extractos vegetales de guanábana el metabolito mayoritario fue el Fitol. Queda demostrado que los aceites esenciales de hojas naranja agria y extractos de hojas de guanábana se pueden constituir como una alternativa agroecológica para el biocontrol de P. cinnamomi.

scholarly journals Biometry of fruit, germination and morphogenesis in vitro of citrus after asepsis of seeds in different concentrations of sodium hypochlorite

2020 ◽  
Vol 13 (3) ◽  
pp. 14
Author(s):  
D. Santos ◽  
W. J. Pereira ◽  
D. S. Miranda ◽  
J. L. C. Souza ◽  
L. A. Borges ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Cultivation ◽  
Sodium Hypochlorite Solution ◽  
Citrus Aurantium ◽  
Federal Institute ◽  
Growth Room ◽  
Morphogenesis In Vitro ◽  
Morphological Processes

In tissue culture it is necessary to use seeds of good quality and free of pathogens in order to avoid damages in the work to be carried out in the laboratory. Therefore, strict cleaning of the seeds intended for in vitro cultivation is essential. Thus, the objective of this study was to evaluate fruit biometry, germination and morphogenesis of 'Cravo' (Citrus limonia Osbeck) and Persia Lima (Citrus aurantium) seeds in vitro submitted to different concentrations of NaClO for asepsis. Fruits of 'Cravo' lemon and Lima of Persia. Subsequently, they were transported to the Biotechnology Laboratory of the Goiano Federal Institute Campus Urutaí-GO, where they performed the biometric characterization of the fruits. The seeds of these fruits were submitted to treatment with sodium hypochlorite solution in different concentrations: 0,0; 1.0; 1.5; 2.0 and 2.5% for 10 minutes, constituting then five treatments for each variety and placed in an acclimatized growth room to analyze the results. The variables evaluated were: biometry of fruits and seedlings; oxidation indexes; germination; contamination; and types of contamination. NaClO doses were compared from 95% confidence intervals. The analyzes were performed using the glm () function of software R version 3.4.1. It was verified that the aseptic treatment with NaClO for lime and lemon was promising for the morphological processes of germination and development of seedlings, as well as for the control of contaminating agents in vitro.

scholarly journals Flavonoids identified from Korean Citrus aurantium L. inhibit Non-Small Cell Lung Cancer growth in vivo and in vitro

2014 ◽  
Vol 7 ◽  
pp. 287-297 ◽  
Author(s):  
Kwang-Il Park ◽  
Hyeon-Soo Park ◽  
Mun-Ki Kim ◽  
Gyeong-Eun Hong ◽  
Arulkumar Nagappan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Cancer Growth ◽  
Citrus Aurantium ◽  
Small Cell Lung

scholarly journals ATIVIDADE ANTIFÚNGICA IN VITRO DE ÓLEOS ESSENCIAIS SOBRE Sphaceloma ampelinum

2015 ◽  
Vol 24 (1) ◽  
pp. 63-70
Author(s):  
Régis De Oliveira Fialho ◽  
Marli De Fátima Stradioto Papa
Keyword(s):  
Vitis Vinifera ◽  
Rosmarinus Officinalis ◽  
Thymus Vulgaris ◽  
Citrus Reticulata ◽  
Citrus Aurantium ◽  
Cinnamomum Zeylanicum ◽  
Cymbopogon Winterianus ◽  
Sphaceloma Ampelinum

O presente trabalho teve como objetivo avaliar a atividade antifúngica in vitro de dez óleos essenciais sobre o crescimento micelial e a germinação de esporos de Sphaceloma ampelinum, agente causal da antracnose da videira. Foram utilizados os óleos essenciais de uva (Vitis vinifera), tangerina (Citrus reticulata), laranja doce (Citrus aurantium), citronela (Cymbopogon winterianus), canela (Cinnamomum zeylanicum), eucalipto (Eucalyptus staigeriana), anis estrelado (Illicium verun), alecrim (Rosmarinus officinalis), cravo (Eugenia caryophyllus) e tomilho branco (Thymus vulgaris) nas concentrações de 0,3 e 1% em relação ao volume total, os quais foram incorporados em meio de cultura ou suspensão de esporos. Determinaram-se as percentagens de inibições do crescimento micelial e da germinação de esporos. Constatou-se que os óleos essenciais de canela, tomilho branco e cravo proporcionaram os melhores efeitos de inibição sobre o crescimento micelial e a germinação de esporos de S. ampelinum e merecem mais estudos, para comprovação de sua eficiência em condições de campo.

scholarly journals Sour orange bud regeneration and in vitro plant development related to culture medium composition and explant type

2010 ◽  
Vol 32 (1) ◽  
pp. 001-008 ◽  
Author(s):  
Rosely Pereira da Silva ◽  
Amancio José de Souza ◽  
Beatriz Madalena Januzzi Mendes ◽  
Francisco de Assis Alves Mourão Filho
Keyword(s):  
Culture Medium ◽  
In Vitro Regeneration ◽  
Medium Composition ◽  
Cultivated Plants ◽  
Sour Orange ◽  
Citrus Aurantium ◽  
Adventitious Buds ◽  
In Vitro Organogenesis ◽  
Bud Regeneration

In order to evaluate the formation of adventitious buds and in vitro regeneration of sour orange plants (Citrus aurantium L.) two organogenesis-inducing experiments were conducted. In the first experiment, the induction and in vitro regeneration of adventitious buds were tested on epicotyl and internodal segments under the influence of BAP or KIN associated with NAA. The second experiment evaluated the in vitro regeneration of sour orange plants related to different explant types (epicotyls segments, internodal segments of in vitro germinated plantlets and internodal segments of greenhouse cultivated plants). Data collected on both experiments included the percentage of responsive explants (explants that formed buds), and the number of buds per explant. The addition of BAP showed the best organogenic response. In vitro germinated epicotyl segments and internodal segments are recommended as explants for sour orange in vitro organogenesis. Rooting of regenerated shoots was achieved without the need of auxin in the medium.

Sign in / Sign up

Export Citation Format

Share Document