scholarly journals Enhancement of Delignification and Glucan Content of Sugarcane Bagasse by Alkali Pretreatment for Bioethanol Production

2021 ◽  
Vol 21 (2) ◽  
pp. 133
Author(s):  
Kyaw Wunna ◽  
Kiohiko Nakasaki ◽  
Joseph Auresenia ◽  
Leonila Abella ◽  
Peg-asa Gaspilo
Keyword(s):  
Sodium Hydroxide ◽  
Residence Time ◽  
Sugarcane Bagasse ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Optimum Parameters ◽  
Two Factors ◽  
Naoh Solution ◽  
Four Levels ◽  
Short Time

The current work aimed to enhance the delignification of sugarcane bagasse (SCB) for bioethanol production. The optimization of alkali (sodium hydroxide) pretreatment parameters such as concentration and residence time was carried out by the Taguchi method using L16 orthogonal array with two factors and four levels. Sugarcane bagasse powder was mixed with sodium hydroxide (NaOH) solution (0.5-2 wt.%) and heated in an autoclave at 121°C and at varied times (30-120 min). From the statistical analysis of data, it was observed that delignification and glucan increased with the increased concentration and short time. The optimum parameters of NaOH pretreatment were 2 wt.% of NaOH concentration and 30 minutes of residence time. At the optimum conditions, 86.8% delignification and 46.6% glucan content of SCB were obtained. Thus, alkali pretreatment optimized by Taguchi design is the effective method to remove lignin and to increase cellulose or glucan content in sugarcane bagasse for facilitating the further catalytic hydrolysis in bioethanol production.

Bioethanol production from agro-industrial Saccharum spp. residues: using Trametes versicolor (L.: Fr.) Quél in simple fermentation and saccharification processes

2020 ◽  
Vol 13 (9) ◽  
Author(s):  
Edna Xochitl Figueroa Rosales ◽  
Erendira Aguilar Huerta ◽  
Manuel Jesús Granados Baeza ◽  
Alfredo Quinto Hernández
Keyword(s):  
Incubation Period ◽  
Sugarcane Bagasse ◽  
Trametes Versicolor ◽  
Reducing Sugars ◽  
Bioethanol Production ◽  
Anova Analysis ◽  
Saccharum Spp ◽  
Commercial Strain ◽  
Naoh Solution ◽  
Factor C

Objective: To quantify the production of total and reducing sugars, as well as bioethanol from sugarcane bagasse (Saccharum spp.) pretreated with Trametes versicolor and 20 % (P/V) NaOH solution. Design/methodology/approach: An experimental 2K design was used to determine the effect of the volume of inoculant added to wild sugarcane bagasse samples (Factor A; 8 and 10 mL), its incubation period (Factor B; 18 and 24 d), and the bagasse weight/ volume of 20% (P/V) NaOH solution (Factor C; 5 and 20%(P/V), on the production of total and reducing sugars during simple saccharification. ANOVA analysis was used to evaluate the significance of these factors. The production of bioethanol was achieved via simple fermentation using a commercial strain of Saccharomyces cerevisiae. Results: It was found that the highest yields of reducing and total sugars were obtained with the use of 10 mL of inoculant solution and with an 18 d incubation period during biological pretreatments. The ANOVA analysis suggests that Factors A and B influence the release of sugars, while Factor C was irrelevant. The bioethanol production registered concentrations of up to 7.3 mg mL-1 for every 5 g of sugarcane bagasse. Limitations/implications: This study focused on how treatments using T. versicolor and NaOH affected the release of sugars. Optimization of bioethanol production needs to be considered subsequently in another study. Findings/conclusions: The results have implications for the use of pretreatments for the production of bioethanol from agro-industrial residues.

PENGARUH KONSENTRASI RAGI Saccharomyces cerevisiae DAN LAMA FERMENTASI TERHADAP KUALITAS CUKA TOMI-TOMI (Flacourtia inermis)

2015 ◽  
Vol 4 (2) ◽  
pp. 50-55
Author(s):  
Sandra J Nendissa ◽  
Rachel Breemer ◽  
Nikholaus Melamas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Dissolved Solids ◽  
Randomized Experimental Design ◽  
Two Factors ◽  
Four Levels

This objectives of this research were both to study and determine the best level of concentration of yeast Saccharomyces cereviseae and period of fermentation on the quality of tomi-tomi vinegar (Flacourtia inermis). A completely randomized experimental design with two factors of treatment was applied in this research. The first factor was concentration of yeast S. cereviseae having four levels of tretament, i.e.: without the addition of yeast 0.5, 1 and 1.5 g yeast. The second factor was period fermentation with 1, 2, 3, 4, and 5 weeks. The result indicated that the concentration of yeast S. cereviseae 1.5 g and period fermentation 5 week produced a good tomi-tomi vinegar with total acids 51.22%, total dissolved solids 8.35, total sugar 8.07% and pH 5.40.

Alkali pretreatment of wheat straw followed by microbial hydrolysis for bioethanol production

2017 ◽  
Vol 40 (9) ◽  
pp. 1203-1211 ◽  
Author(s):  
Bahiru Tsegaye ◽  
Chandrajit Balomajumder ◽  
Partha Roy
Keyword(s):  
Wheat Straw ◽  
Bioethanol Production ◽  
Alkali Pretreatment ◽  
Microbial Hydrolysis

scholarly journals PRODUÇÃO DE CELULASES E XILANASE POR Aspergillus niger IZ9 USANDO FERMENTAÇÃO SUBMERSA SOBRE BAGAÇO DE CANA-DE-AÇÚCAR

2000 ◽  
Vol 18 (1) ◽  
Author(s):  
CLAUDIO LIMA AGUIAR ◽  
TOBIAS J. B. MENEZES
Keyword(s):  
Aspergillus Niger ◽  
Phosphoric Acid ◽  
Sodium Hydroxide ◽  
Sugarcane Bagasse ◽  
Sodium Hydroxide Solution ◽  
Cellulolytic Enzymes ◽  
Hydroxide Solution ◽  
Chemically Treated

Avaliou-se a produção de celulases e xilanase de Aspergillus niger IZ9, crescido sobre bagaço de cana, quimicamente tratado, como substrato. Os tratamentos foram: solução de hidróxido de sódio a 4%, e solução de hidróxido de sódio a 4%, ácido fosfórico p.a. e vapor. A produção das enzimas celulolíticas (celulase total, endoglicanase e ­glicosidase) e xilanase foi observada nos bagaços tratados e nãotratado. O tratamento com solução de hidróxido de sódio a 4% promoveu maior indução de síntese da maioria das enzimas, com exceção de ­glicosidase, a qual apresentou produção semelhante para os bagaços tratados quimicamente. Abstract It was evaluated the production of cellulases and xylanase by Aspergillus niger IZ09 grown in a substrate consisted of chemically treated sugarcane bagasse. The treatments were: 4% sodium hydroxide solution and 4% sodium hydroxide solution, phosphoric acid and steam. The production of the cellulolytic enzymes (total cellulase, endoglycanase and B.CEPPA, Curitiba, v. 18, n. 1, jan./jun.2000 67 ­glucosidase) and xylanase was observed in the treated and nontreated bagasses. The treatment with 4% sodium hydroxide solution promoted a greater induction of the synthesis of the majority of the enzymes, with exception of ­glucosidase, which showed similar production for both chemically treated bagasses.

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