scholarly journals The Food poisoning outbreak caused by diarrhoeal Bacillus Cereus in Tegalkenongo Village, Bantul, Yogyakarta, Indonesia: a retrospective study

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kornelius Son
Keyword(s):  
Klebsiella Pneumoniae ◽  
Bacillus Cereus ◽  
Incubation Period ◽  
Causative Agent ◽  
Food Poisoning ◽  
Field Investigation ◽  
Epidemiological Investigation ◽  
Adjusted Risk ◽  
Adjusted Risk Ratio ◽  
Causative Agents

Background: On May, 13 2019, a food poisoning outbreak of diarrhoeal B. cereus associated with contaminated chicken satay occurred in Tegalkenongo village involving villagers after attending mass iftar in a mosque. Health office of Bantul District revealed two attendances were hospitalized in the hospital of PKU Muhammadiyah after consuming food served during mass iftar. Based on the information, we immediately conducted an epidemiological investigation to make sure of the existence of the outbreak. Objectives: This study aimed to investigate the causative agents, source of food poisoning and mode of food poisoning transmission in Tegalkenongo Village. Methods: A retrospective cohort study was used in the epidemiological investigation during one week from 13 May 2019 – 20 May 2019. Results: Based on the investigation, Of the 303 villagers involved in the event, 188 villagers were ill with the median age of cases was 38 years old, the average incubation period was 8 hours, and the predominate symptoms were diarrhea (93.62%), nausea (84.57%), and abdominal cramps (64.89%). Contaminated chicken satay was determined as the source of contamination with adjusted Risk Ratio (aRR) was 4.36; 95% CI 1.1538, 16.5285. Initial epidemiological features and cultures from food items served in the event and stool sample of one patient suggested that the causative agent was Bacillus Cereus which was supported by Klebsiella Pneumoniae. Conclusion: Based on the field investigation result related to symptoms and incubation period and laboratory identification, we conclude that the causative agent was diarrhoeal B. cereus. Keywords: Food poisoning, Bacillus cereus, Klebsiella pneumoniae

An Epidemiological Investigation on an Outbreak of Bacillus Cereus Food Poisoning in a Girls' High School in Sangju-si, Korea, 2008.

2008 ◽  
Vol 30 (2) ◽  
pp. 168-177 ◽  
Author(s):  
Hyun Dong Lee ◽  
Sun Ok Lee ◽  
Hyun Sul Lim ◽  
Kyoung Chan Lee ◽  
Kyu Jin Chang ◽  
...  
Keyword(s):  
High School ◽  
Bacillus Cereus ◽  
Food Poisoning ◽  
Epidemiological Investigation

scholarly journals Evaluation of growth and cereulide production by Bacillus cereus isolated from cooked rice

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Damilola O. Seyi-Amole ◽  
Abiodun A. Onilude ◽  
Dasari S. Rani ◽  
Prakash M. Halami
Keyword(s):  
Bacillus Cereus ◽  
Incubation Period ◽  
Food Poisoning ◽  
Optimum Growth ◽  
Ph Values ◽  
Cooked Rice ◽  
Incubation Periods ◽  
Different Temperatures ◽  
Static Conditions ◽  
Incubation Temperatures

Conditions influencing Bacillus cereus growth and cereulide production, such as temperature and pH, were evaluated at varying incubation periods. The growth and cereulide production at different temperatures and pH values ranging from 10 to 40 ºC and 5.0 to 8.5, respectively showed that the temperature from 20 to 30 ºC and at pH from 6.0 to 7.0 gave the optimum growth and cereulide production by B. cereus SA105. pH below 6.0 resulted in reduced growth and cereulide production. Cereulide production increased along with the incubation period, and maximum cereulide titre (ng/mL) of 1219.1±8.90 was obtained after 6 days of incubation at 30 ºC and pH 6.5 under static conditions. There was no quantifiable toxin at incubation temperatures of 10 and 40 ºC by B. cereus SA105. This work further reveals that B. cereus growth and cereulide production was significantly affected by temperature and pH in relation to the incubation period. Furthermore, the findings of this study will serve as a means for reducing the diversity of emetic toxin-producing B. cereus population in food and food products, thus preventing food poisoning.

scholarly journals Bacillus-infecting bacteriophage Izhevsk harbors thermostable endolysin with broad range specificity

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242657
Author(s):  
Anna V. Skorynina ◽  
Emma G. Piligrimova ◽  
Olesya A. Kazantseva ◽  
Vladislav A. Kulyabin ◽  
Svetlana D. Baicher ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Infections ◽  
Bacterial Species ◽  
Food Poisoning ◽  
Broad Host Range ◽  
Lytic Enzymes ◽  
Transmission Electron ◽  
Causative Agents ◽  
Primary Means ◽  
Spectrum Activity

Several bacterial species belonging to the Bacillus cereus group are known to be causative agents of food poisoning and severe human diseases. Bacteriophages and their lytic enzymes called endolysins have been widely shown to provide for a supplemental or primary means of treating bacterial infections. In this work we present a new broad-host-range phage Izhevsk, which infects the members of the Bacillus cereus group. Transmission electron microscopy, genome sequencing and comparative analyses revealed that Izhevsk is a temperate phage with Siphoviridae morphology and belongs to the same genus as the previously described but taxonomically unclassified bacteriophages Tsamsa and Diildio. The Ply57 endolysin of Izhevsk phage has broad-spectrum activity against B. cereus sensu lato. The thermolability of Ply57 is higher than that of the PlyG of Wβ phage. This work contributes to our current understanding of phage biodiversity and may be useful for further development of efficient antimicrobials aimed at diagnosing and treating infectious diseases and food contaminations caused by the Bacillus cereus group of bacteria.

scholarly journals A Salmonella-related foodborne outbreak in a snack bar in Jeju Province: an epidemiological investigation

2021 ◽  
Vol 18 (2) ◽  
pp. 25-30
Author(s):  
Eun-Suk Cho ◽  
Seung Hyuk Lee ◽  
Jong-Myon Bae
Keyword(s):  
Incubation Period ◽  
Environmental Samples ◽  
Food Poisoning ◽  
Infectious Agent ◽  
Epidemiological Investigation ◽  
Prevention Measures ◽  
Additional Food ◽  
Local Public Health ◽  
Epidemic Curve ◽  
Phone Calls

Many people reported suspected food poisoning after consuming food at the same snack bar on June 18, 2020. Thus, an in-depth epidemiological investigation was conducted to identify the infectious agent and establish additional food poisoning prevention measures. The study included people who reported to the local public health center after June 18 with acute gastroenteritis symptoms within 4 days of consuming food from the snack bar. The onset of symptoms and food items consumed by individuals were then investigated via phone calls and on-site visits. Afterward, the infectious agent was identified from human samples (stool or rectal swab) of four restaurant employees and 89 people and from environmental samples (materials, cooking utensils, and water). The analysis revealed that the incubation period ranged from 2 hours to 92 hours, with a median and mode of 16 hours and 12 hours, respectively. Moreover, the epidemic curve had a unimodal shape because of common exposure, which reached its peak on June 18. After monitoring for 8 days, which is more than twice the maximum incubation period of 92 hours, the end of the epidemic was declared on June 28 as no additional cases were reported. Analysis of human and environmental samples revealed Salmonella bareilly of the pulsed-field gel electrophoresis pulsotype SAPX01.017 as the causative agent. Therefore, it was concluded that the food poisoning outbreak was caused by S. bareilly.

scholarly journals Evaluation of growth and cereulide production by Bacillus cereus isolated from cooked rice

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Damilola O. Seyi-Amole ◽  
Abiodun A. Onilude ◽  
Dasari S. Rani ◽  
Prakash M. Halami
Keyword(s):  
Bacillus Cereus ◽  
Incubation Period ◽  
Food Poisoning ◽  
Optimum Growth ◽  
Ph Values ◽  
Cooked Rice ◽  
Incubation Periods ◽  
Different Temperatures ◽  
Static Conditions ◽  
Incubation Temperatures

Conditions influencing Bacillus cereus growth and cereulide production, such as temperature and pH, were evaluated at varying incubation periods. The growth and cereulide production at different temperatures and pH values ranging from 10 to 40 ºC and 5.0 to 8.5, respectively showed that the temperature from 20 to 30 ºC and at pH from 6.0 to 7.0 gave the optimum growth and cereulide production by B. cereus SA105. pH below 6.0 resulted in reduced growth and cereulide production. Cereulide production increased along with the incubation period, and maximum cereulide titre (ng/mL) of 1219.1±8.90 was obtained after 6 days of incubation at 30 ºC and pH 6.5 under static conditions. There was no quantifiable toxin at incubation temperatures of 10 and 40 ºC by B. cereus SA105. This work further reveals that B. cereus growth and cereulide production was significantly affected by temperature and pH in relation to the incubation period. Furthermore, the findings of this study will serve as a means for reducing the diversity of emetic toxin-producing B. cereus population in food and food products, thus preventing food poisoning.

Glycaemic responses to glucose and rice in people of Chinese and European ethnicity

2020 ◽  
Author(s):  
M Kataoka ◽  
BJ Venn ◽  
SM Williams ◽  
Lisa Te Morenga ◽  
IM Heemels ◽  
...  
Keyword(s):  
Ethnic Differences ◽  
Rice Variety ◽  
Glycaemic Index ◽  
Activity Levels ◽  
Glycaemic Response ◽  
Rice Varieties ◽  
Adjusted Risk ◽  
Postprandial Glycaemia ◽  
Adjusted Risk Ratio ◽  
Glucose Curve

Aims: Diabetes rates are especially high in China. Risk of Type 2 diabetes increases with high intakes of white rice, a staple food of Chinese people. Ethnic differences in postprandial glycaemia have been reported. We compared glycaemic responses to glucose and five rice varieties in people of European and Chinese ethnicity and examined possible determinants of ethnic differences in postprandial glycaemia. Methods: Self-identified Chinese (n = 32) and European (n = 31) healthy volunteers attended on eight occasions for studies following ingestion of glucose and jasmine, basmati, brown, Doongara® and parboiled rice. In addition to measuring glycaemic response, we investigated physical activity levels, extent of chewing of rice and salivary α-amylase activity to determine whether these measures explained any differences in postprandial glycaemia. Results: Glycaemic response, measured by incremental area under the glucose curve, was over 60% greater for the five rice varieties (P < 0.001) and 39% greater for glucose (P < 0.004) amongst Chinese compared with Europeans. The calculated glycaemic index was approximately 20% greater for rice varieties other than basmati (P = 0.01 to 0.05). Ethnicity [adjusted risk ratio 1.4 (1.2-1.8) P < 0.001] and rice variety were the only important determinants of incremental area under the glucose curve. Conclusions: Glycaemic responses following ingestion of glucose and several rice varieties are appreciably greater in Chinese compared with Europeans, suggesting the need to review recommendations regarding dietary carbohydrate amongst rice-eating populations at high risk of diabetes. © 2012 Diabetes UK.

KEJADIAN LUAR BIASA KERACUNAN “CUMI-CUMIAN” DI SEKOLAH DASAR NEGERI 1 TRASAN BANDONGAN KABUPATEN MAGELANG

2018 ◽  
Vol 7 (2) ◽  
pp. 131-136
Author(s):  
Nasir Ahmad
Keyword(s):  
Abdominal Pain ◽  
Bacillus Cereus ◽  
Rhodamine B ◽  
Attack Rate ◽  
Food Poisoning ◽  
Health Department ◽  
Analysis Data ◽  
Food Samples ◽  
Bivariate Analysis ◽  
School Committee

Background: On May 4th, 2016, at 12:30 district surveillance officer of Magelang Health Department received reports from Public Health Center of Bandongan about 21 students of SDN 1 Trasan who suffered from the same food-poisoning symptoms. Objective: Investigation was carried out to identify the source, how it spread and how to control it. Methods: This study used descriptive analytic and mapping the cases distribution location. The case was people experiencing symptoms of dizziness or abdominal pain or nausea or vomiting. Data analysis was done by using bivariate analysis. Data collection were done through interviews, observations and laboratory tests on the food samples. Results: The case was 50 students (from 1-6 grade students). The perceived symptoms were dizziness (77%), nausea (42%), abdominal pain (40%) and vomiting (8%). Attack rate found ranged from 14.3% to 60% with the highest Attack rate found on class three (60%). The incubation period of 15-240 minutes (mean 72.3 minutes). Calamari like positive Bacillus cereus and Rhodamine-B 10 mg/kg. Conclusion: The outbreak of food poisoning because calamari like contaminated Bacillus cereus. We suggested the school committee to provide the socialization of harmful food for the students. The teachers should restrict the permission for the food vendor to sell at school.   Keywords: Bacillus cereus, , Food Poisoning, Outbreak, Rhodamine B, School Food

Fungus Spaeropsis Malorum peck. One of the currant stalk drying agents

2020 ◽  
Vol 62 ◽  
pp. 172-178
Author(s):  
S. E. Golovin
Keyword(s):  
Causative Agent ◽  
High Frequency ◽  
Frequency Of Occurrence ◽  
Ribes Nigrum ◽  
Black Currant ◽  
Causative Agents ◽  
Pear Trees ◽  
Red Currant

The article highlights the results of research carried out in 2019-2020 to study the reasons for the drying out of the stems of black currant (Ribes nigrum L.), red currant (Ribes rubrum L.) and golden currant (Ribes aureum L.). As a result of research, the fungus Sphaeropsis malorum Peck. in the first in Russia it was revealed from the drying stems of three types of black currants, red currants and golden currants. This  pathogen inhabited currant stems together with fungi from the genus Cytospora spp., causative agents of stem drying. On red currants, it was established that S. malorum was the main causative agent of branch drying, as indicated by the high frequency of occurrence (75.0-83.3 %) of this fungus on the affected stems, compared with species from the genus Cytospora spp. (16.7-33.3 %) and Phomopsis ribesia (25.0 %). Due to the fact that the cultivation of currants in the immediate vicinity of apple and pear trees leads to infection of plants with the fungus S. malorum, it is necessary to avoid planting currants in the vicinity of fruit-bearing seed gardens.

scholarly journals Development of an Immunochromatographic Strip Using Conjugated Gold Nanoparticles for the Rapid Detection of Klebsiella pneumoniae Causing Neonatal Sepsis

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1141
Author(s):  
Noha M. Elhosseiny ◽  
Tamer M. Samir ◽  
Aliaa A. Ali ◽  
Amani A. El-Kholy ◽  
Ahmed S. Attia
Keyword(s):  
Gold Nanoparticles ◽  
Klebsiella Pneumoniae ◽  
Causative Agent ◽  
Neonatal Sepsis ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Immunochromatographic Strip ◽  
Amino Acid Peptide ◽  
Negative Results ◽  
Drug Resistant Bacteria

Neonatal sepsis is a leading cause of death among newborns and infants, especially in the developing world. The problem is compounded by the delays in pinpointing the causative agent of the infection. This is reflected in increasing mortality associated with these cases and the spread of multi-drug-resistant bacteria. In this work, we deployed bioinformatics and proteomics analyses to determine a promising target that could be used for the identification of a major neonatal sepsis causative agent, Klebsiella pneumoniae. A 19 amino acid peptide from a hypothetical outer membrane was found to be very specific to the species, well conserved among its strains, surface exposed, and expressed in conditions simulating infection. Antibodies against the selected peptide were conjugated to gold nanoparticles and incorporated into an immunochromatographic strip. The developed strip was able to detect as low as 105 CFU/mL of K. pneumoniae. Regarding specificity, it showed negative results with both Escherichia coli and Enterobacter cloacae. More importantly, in a pilot study using neonatal sepsis cases blood specimens, the developed strip selectively gave positive results within 20 min with those infected with K. pneumoniae without prior sample processing. However, it gave negative results in cases infected with other bacterial species.

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