scholarly journals Chromosome status and yield characteristics of soybean (Glycine max L. (Merr)) in saline soil as affected by induced mutation

2021 ◽  
Vol 6 (2) ◽  
pp. 124
Author(s):  
Agus Pernando Simanjuntak ◽  
Diana Sofia Hanafiah ◽  
Luthfi Aziz Mahmud Siregar

To fulfill the demand of soybeans, the expansion of the planting area can be one of the choices through the conversion of marginal land by utilizing suboptimal land, such as saline soil. One of the plant breeding techniques to obtain tolerant plants to salinity is the use of chemical mutations, such as colchicine. The study aimed to reveal the effect of salinity on the characters of soybean plants (mutated with colchicine) and the effect of colchicine on the number of chromosomes and ploidy level. This study was conducted from January to March 2020 and carried out in the plastic house of Faculty of Agriculture, Universitas Sumatera Utara. The experiment was arranged in a Randomized Block Design with salinity levels (0 dS.m-1, 2 dS.m-1, 4 dS.m-1, 6 dS.m-1) as treatments and consisted of ten replications in each treatment. The result showed that the soybean plants were not successfully induced by colchicine at a concentration of 0.04 % with soaking duration of 10 hours, as indicated by the number of chromosomes and analysis of ploidy level. The soybeans that had been induced were planted in saline soil. There were significant effects observed on the productive branch, number of filled pods, and the weight of seeds per plant. Colchicine treatment did not successfully affect the number of chromosomes of soybeans but had an impact on the chromosome pattern. In addition, the salinity treatment of 4 dS.m-1 significantly affected the number of filled pods compared to the treatment of 6 dS.m-1.

2020 ◽  
Vol 25 (3) ◽  
pp. 349-355
Author(s):  
Fitri Krismiratsih ◽  
Sugeng Winarso ◽  
Slamerto Slamerto

Efforts to increase production potential can be carried out by extensification in a less productive saline land. Salinity is a major problem in the growth of most plants. Azolla is a plant that is sensitive to salinity, but if it is applied well, it can grow optimally at high salinity levels. The purpose of this study is to obtain an azolla application technique that is effective in increasing the adaptation of rice plants to NaCl saline soil conditions. The experimental design used was Randomized Block Design (RBD) with 2 factors and 3 replications. The first factor was the azolla application technique consisted of 3 levels: fresh azolla composted, fresh azolla immersed, and fresh azolla as a ground cover. The second factor was the levels of NaCl salt stress consisted of 4 levels: control DHL 0, 2, 4, and 8 dS m-1. The adaptation ability of rice plants based on variable plants height growth rate, number of tillers, strove dry weight, root dry weight, stomata density, leaf chlorophyll (SPAD), age of flowering, number of paddy grain, and harvest index. The results showed how to test content up to 2 dS m-1 which increased rice growth especially the application of azolla composted. Increasing stress to 4 and 8 dS m-1showed bad effects on vegetative, physiology, and yields of rice components. The stronger of salt stress the higher all plants growth variables except the age of flowering that actually showed the acceleration of flowering. Application of composted azolla can increase the root dry weight and azolla as a ground cover can increase the numbers of paddy grains.   Keywords: azolla, NaCl, rice, stress


2020 ◽  
Vol 50 (9) ◽  
Author(s):  
Alfredo Junior Paiola Albrecht ◽  
Leandro Paiola Albrecht ◽  
André Felipe Moreira Silva ◽  
Romulo Augusto Ramos ◽  
Natália Buttini Corrêa ◽  
...  

ABSTRACT: Conyza spp. is among the main weeds reported worldwide. Due to its aggressiveness, such as high seed production and dispersion, and the growing reports of biotypes resistant to glyphosate, paraquat, and other herbicides, different control practices are required. Thus, this study aimed to evaluate the efficacy of herbicides with sequential application of glufosinate in soybean pre-sowing for control of Conyza spp. with indicative of resistant to paraquat. The study was carried out in the field, at Assis Chateaubriand and Palotina, state of Paraná, Brazil, in the 2018/19 season. The experiments were conducted in a randomized block design with four replications. The treatments consisted in application of glyphosate, 2,4-D, saflufenacil, glufosinate, saflufenacil/imazethapyr, diclosulam, paraquat/diuron, paraquat and imazethapyr/flumioxazin, at different combinations, in soybean pre-sowing. Control of Conyza spp., crop injury to soybean plants and variables related to agronomic performance (plant height and yield) were evaluated. All treatments were selective for soybean, which showed stronger crop injury in the presence of diclosulam herbicide, but this did not compromise soybean agronomic performance. In general, control levels were high for the treatments used. Except for paraquat treatments, in the area with the highest frequency of Conyza spp. with indicative of resistant to paraquat, and imazethapyr/flumioxazin treatment in both areas. These control results emphasized the importance of glufosinate in this management system and showed promising results for saflufenacil/imazethapyr.


Author(s):  
M. V. Dlamini ◽  
M. T. Masarirambi

Saline irrigation water is becoming an important water source as fresh water is fast becoming a scarce resource in many areas of the world, including Eswatini, especially in arid and semi-arid regions.  A study to test the response of two varieties of spinach (fordhook giant and mustard) to salinity was conducted in a field pot experiment at the Faculty of Agriculture at the Luyengo Campus of the University of Eswatini.  The treatments were laid in a randomized block design (RCBD).  The experiment consisted of four treatments, each replicated twelve times.  Treatments were salinity levels of 0.0 dS/m, 1.5 dS/m, 2.0 dS/m and 3.5 dS/m.  All the treatments were subjected to similar agronomic practices. Spinach was grown and observed for a period of five weeks.  Plant height was measured and the number of leaves counted weekly throughout the experiment. Significant differences (P < 0.05) between salinity treatments were obtained for plant height beginning in week 2 but were more pronounced in week 3, 4 and week 5.  No significant differences were obtained for the number of leaves.  There were however, clear significant differences between spinach irrigated with none saline irrigation water compared to saline irrigation water.   It was concluded that irrigating spinach with saline water of more than 2.0 dS/m drastically reduce plant growth but not the number of leaves under the conditions of the experiment.


2020 ◽  
Vol 4 (1) ◽  
pp. 108-117
Author(s):  
Rahmat Fitra Yandi Nasution ◽  
Syamsuddin Syamsuddin ◽  
Syafruddin Syafruddin

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh jenis mikoriza, varietas dan interaksi jenis mikoriza dan varietas terhadap pertumbuhan dan hasil tanaman cabai pada tanah Incepticol. Penelitian ini dilaksanakan di Kebun Percobaan Iesuum Krueng Raya Aceh Besar, Rumah Kaca dan Laboratorium Fisiologi Tumbuhan Fakultas Pertanian Universitas Syiah Kuala Darussalam Banda Aceh dari bulan Mei sampai September 2018. Analisis data yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) pola faktorial 3 x 2 dengan 3 ulangan. Faktor yang diamati dalam penelitian ini adalah jenis mikoriza dan varietas. Jenis mikoriza yang digunakan adalah Glomus mosseae, Gigaspora sp dan Campuran serta varietas yang digunakan adalah varietas Lado F1 dan Perintis. Hasil penelitian ini menunjukan jenis mikoriza berpengaruh nyata pada tinggi tanaman 30 dan 45 HSTdan jumlah cabang produktif. Jenis mikoriza terbaik terdapat pada jenis mikoriza campuran (Glomus mosseae dan Gigaspora sp). Pada varietas berpengaruh sangat nyata terhadap tinggi tanaman 15,30 dan 45 HST, diameter batang 30 dan 40 HST dan jumlah cabang produktif serta berpengaruh nyata pada diameter batang 45 HST. Jenis varietas terbaik terdapat pada varietas Lado. Interaksi antara jenis mikoriza dan jenis varietas berpengaruh sangat nyata terhadap tinggi tanaman 30 HST dan jumlah cabang produktif serta berpengaruh nyata pada diameter batang 30 HST.Interaksi antara jenis mikoriza dan varietas terbaik tedapat pada jenis mikoriza campuran (Glomus mosseae dan Gigaspora sp) dengan varietas Lado F1.The Effect of Mycorrhizal Types on the Growth of Some Chili Varieties in Incepticol Soil Krueng Raya Aceh BesarAbstract. This research aims to determine the effect of type of mycorrhiza, varieties, and interactions of mycorrhiza and varieties types on the growth and yield of chili plants on the Inceptisol land. This research was carried out at the Krueng Raya Iesuum Experimental Garden in Aceh Besar, greenhouse and laboratory of plant physiology, Faculty of Agriculture of Syiah Kuala University, Darussalam, Banda Aceh from Mei to September 2018. The analysis of data used in this study was Randomized Block Design -Factorial 3x2 with 3 replications. The factors observed in this research were the type of mycorrhiza and varieties. Types of mycorrhiza used are Glomus mosseae, Gigaspora, and the combination. Varieties used are Lado F1 and Perintis. The result of this research showed that types of mycorrhiza take effect significantly on plant height 30 dan 45 DAP and productive branch numbers. The mixed of mycorrhiza gives the best result on growth and yield of chili plant. The varieties of chili plants highly significant take effect on plant height 15, 30 and 45 DAP, stem diameter 30 and 40 HST and productive branch numbers and significantly affect on stem diameter 45 DAP. Lado F1 gives the best result on growth and yield. The interactions of mycorrhiza and varieties type highly significant take effect on plant height 30 HST and productive branch number and significantly take effect on stem diameter 30 HST. The combination of mixed mycorrhiza and Lado F1 treatment gives the best result on growth and yield of chili plants. 


EUGENIA ◽  
2015 ◽  
Vol 21 (1) ◽  
Author(s):  
J. Rimbing ◽  
J. Pelealu ◽  
D. Sualang

ABSTRACT   The research was arranged in a randomized block design (RBD), with 10 treatments and 3 replications. To get the active insects pests, net sweepings were done for the flying insects, while other pests were observed directly on the plants. Collecting data of plant damages were made by systematic diagonal lines, except for viruses, census was conducted on each experimental plot.  The results of this experiment documented 12 species of insect pests attacking soybean plants. Soybean pest populations were relatively low, except for Aphis glycine that was quite high. In all treatments, plant damage caused by sucking pest Piezodorus sp and Riptortus linearis were relatively low of 1.53 % to 1.94 %; it indicated an insignificant effect to decline the production. Crop damages by virus showed a significant effect. The lowest virus attacks found on compost of 5 tons per ha with 43 plants infected per treatment, the highest virus attack showed on combination of 5 tons compost per ha + inorganic fertilizer + mulch, in which there were 274 infected plants per treatment. Apparently virus attacks gave impact on soybean production. The highest production were documented on treatment of 5 tons compost per ha with production of 0.55 tons per ha, while the lowest production was found on combination of 5 tons compost per ha + inorganic fertilizer + mulch, in which the production was 0.22 tons per ha. Keywords:  fertilizer, mulch, insect pest, production


Author(s):  
Claudivan F. de Lacerda ◽  
Jorge F. da S. Ferreira ◽  
Donald L. Suarez ◽  
Emanuel D. Freitas ◽  
Xuan Liu ◽  
...  

ABSTRACT The aim of this study was to evaluate the accumulation of salts in the soil from irrigation water and of N and K from fertilization. The experiment was conducted in PVC columns (20 cm in diameter and 100 cm in height), filled with non-saline soil, and cultivated with maize. A completely randomized block design in a 4 x 4 factorial was used, with four levels of salinity (0.5, 2.5, 5.0 and 7.5 dS m-1), four N rates, and five replicates. Nitrogen was applied as urea and potassium nitrate at the following rates: N1: N recommendation for maize (2.6 g column-1); N2: 0.3 times (0.78 g column-1) the recommended N1 dose; N3 and N4 with N based on N1 and N2 doses, respectively, reduced proportionally based on the evapotranspiration reduction caused by salinity. After 74 days from sowing, root and soil samples were collected at different soil depths. The electrical conductivity of the saturated extract (ECe) and the concentration of ions (Ca2+, Na+, and Cl-) increased as a function of salinity and soil depth. The opposite was observed for the root system. The increase in salinity also resulted in K+ and NO3- accumulation in the soil column, mainly in treatments with higher N rates (N1 and N3). At the end of the experiment, 88% of the NO3- applied at the highest salinity treatment (7.5 dS m-1) and the highest N rate (N1) was below 20 cm soil depth, evidencing a N loss process caused by leaching.


2014 ◽  
Vol 60 (No. 10) ◽  
pp. 439-445 ◽  
Author(s):  
A. Ashrafi ◽  
M. Zahedi ◽  
K. Fahmi ◽  
R. Nadi

Bioaccumulation of heavy metals can be affected by various crop-weed interactions in agroecosystems. An experiment was conducted to evaluate the role of belowground interaction of soybean and purslane (Portulaca oleracea L.) weed on cadmium (Cd) uptake and its allocation to soybean grains. The experimental treatments included two cropping systems (mono and mixed culture), two salinity levels (0% and 0.5% NaCl) and three levels of Cd in soil (control; 3 and 6 mg Cd/kg). Results showed that the promoting effect of salinity on Cd uptake by soybean and Cd allocation to grains was enhanced in the presence of purslane compared to the absence of neighbour plant. This could be due to increasing Cd-mobilization within the shared rhizosphere of plants. In the non-saline soil decreasing uptake and grain allocation of Cd in co-planted soybean was associated with enhancing of purslane Cd uptake and the depletion of Cd in soil solution. Therefore, it can be concluded that co-planted purslane can alter the uptake of cadmium to the neighboring soybean plants; its effect may be influenced by soil environmental conditions such as salinity.


Revista CERES ◽  
2013 ◽  
Vol 60 (5) ◽  
pp. 621-628 ◽  
Author(s):  
Gederson Luiz Buzzello ◽  
Michelangelo Muzell Trezzi ◽  
José Abramo Marchese ◽  
Elouize Xavier ◽  
Edemir Miotto Junior ◽  
...  

Soybean genotypes grown in sub-tropical climate may exhibit lodging. The plant lodging is influenced by soil type and fertility level, sowing date, latitude and altitude of the location, plant population and conditions of crop development. Plant regulators and herbicides are able to avoid or reduce plant lodging. This study aimed to verify the effects of the growth regulators TIBA and daminozide on vegetative growth and yield of soybean cultivar CD 214 RR. The experiment was carried out at a field in randomized block design with four replications in a factorial scheme. The A factor was represented by the combination of regulators TIBA and daminozide and its concentrations, and the Factor B was seven times of evaluation of injury and plant height or eight times of evaluation of lodging. In the range of doses used, the application of daminozide resulted in greater injury to soybean plants than TIBA. The smaller plant height was achieved by the application of 6 g ha-1 of TIBA and 1200 g ha-¹ of daminozide. Treatments with daminozide (100 g ha-¹) and TIBA (10 g ha-1) stood out due to the reduced lodging of soybean plants. Grain weight increased linearly when the levels of TIBA increased. There was a negative correlation between lodging and grain yield and a positive correlation between plant height and lodging. There was also a negative correlation between injury caused by the application of plant regulators and lodging.


2010 ◽  
Vol 58 (3) ◽  
pp. 313-321 ◽  
Author(s):  
P. Banerjee ◽  
P. Kole

Seven parents (CST2002, MT34, OS-Sel-2, TKG22, AAUDT9304-14-4, B67 and Rama), their 21 F 1 s and 21 F 2 s were grown in summer 2003 in a randomized block design with three replications. Heterosis and inbreeding depression were studied for seven important yield-contributing characters (plant height, branch number plant −1 , capsules plant −1 , seeds capsule −1 , 1000-seed weight, stick yield plant −1 and seed yield plant −1 ). Maximum heterosis for seed yield plant −1 over the mid- and better-parent was recorded in CST2002×TKG22 (43.30%) and MT34×B67 (27.22%), respectively. Mid-parent heterosis for seed yield plant −1 was due to cumulative heterosis for various important component traits, such as capsules plant −1 , seeds capsule −1 and 1000-seed weight. Inbreeding depression was highest for seed yield, followed by 1000-seed weight, capsules plant −1 , branch number and plant height, indicating the predominance of non-additive genetic effects. B67×Rama exhibited significant positive heterosis in F 1 , but non-significant inbreeding depression in F 2 for seed yield. This cross can be utilized as basic material for identifying better pure lines. The clustering pattern indicated that in general genetically diverse parents exhibited more heterosis, as evident in the majority of the crosses.


Author(s):  
Juwarno Juwarno ◽  
Tata Brata Suparjana ◽  
Muachiroh Abbas

Mahameru cultivar is high salinity tolerant cultivar. The previous study result showed Mahameru cultivar could tolerate 140mM NaCl, but Cilacap Coast salinity levels often reaching 200mM NaCl. A research of salinity stress on Mahameru cultivar at 200 mM NaCl have not conducted yet. Therefore to conduct the research of Mahameru at high salinity stress to obtained high salinity tolerant soybean cultivar.   The observed variables are anatomy (epidermis thickness, the density of stomata and trichomes, palisade thickness) physiology (the dry weight of roots and canopy, the content of chlorophyll a and b) Production (whole pod, total filled pod, total empty pod, weight per one-hundred beans). The salinity treatment was 0, 50,100, 150, 200 mM NaCl given at three days before planting and twenty-one days after planting. The data of anatomy and physiology was taken at forty-five days after planting. The production data was taken when soybean plants turned brown. The result indicates that salinity affects anatomy characteristic of leaf, higher the salinity increasing epidermis thickness and the density of stomata and trichomes. Salinity affected the content of chlorophyll a and b. Higher the salinity increased the content of chlorophyll a and b. Salinity did not affect soybean production. Based on this study Mahameru cultivar is resistant to salinity up to 200 mM NaCl. The benefit of this research help to enhance national soybean production with utilization coastal land for soybean planting Mahameru cultivar.         


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