scholarly journals Potensi Antibakterial Bakteri Asam Laktat dari Peda, Jambal Roti, dan Bekasam

2006 ◽  
Vol 8 (2) ◽  
pp. 153
Author(s):  
Ninoek Indriati ◽  
Indriarto P. Danan Setiawan ◽  
Yulneriwarni Yulneriwarni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Diffusion Method ◽  
Fish Products ◽  
Fermented Fish ◽  
E Coli ◽  
Inhibition Zones ◽  
And Staphylococcus Aureus

Isolation of lactic acid bacteria from fermented fish products, peda, jambal roti and bekasam were carried out. Antibacterial activity of lactic acid bacteria was tested using well diffusion method against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus. Twelve isolates of lactic acid bacteria had been isolated. Results showed that isolate A2 from peda was able to inhibit the growth of the 4 testing bacteria with inhibition zones of 6.7 mm for E. coli; 7.3 mm for P. aeruginosa; 8.3 mm for S. aureus and 10.0 mm for B. cereus. Isolate B5 from jambal roti had inhibition zones 11.6 mm for E. coli; 6.0 mm for P. aeruginosa ; 7.3 mm for S. aureus and 13.3 mm for B. cereus ; while isolate C6 from bekasam had inhibitor zones 7.7 mm for E. coli; 6.0 for P. aeruginosa; 8.0 mm for S. aureus and 9.3 mm for B. cereus.

scholarly journals CHARACTERIZATION OF LACTIC ACID BACTERIA AND ANTIMICROBIAL ACTIVITY IN SUI WU’U FROM BAJAWA DISTRICT, NUSA TENGGARA TIMUR, INDONESIA

2020 ◽  
pp. 44-49
Author(s):  
ROSALINA YULIANA AYEN ◽  
ENDANG KUSDIYANTINI ◽  
SRI PUJIYANTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rrna ◽  
Diffusion Method ◽  
E Coli ◽  
Query Cover

Objective: This research aimed to isolate, determine the characteristics of lactic acid bacteria (LAB) of Sui Wu’u from Bajawa, Nusa Tenggara Timur and identify LAB using 16S rRNA potential as antimicrobial activity against pathogenic bacteria. Methods: Sui Wu’u which has been stored for 6 months was obtained from Bajawa district, inoculated on de Man Rogosa-Sharpe Agar (Merck) + 0.5% CaCO3, purification of LAB, characterization of selected isolates, biochemical test, tolerance test for pH, viability to test temperature, and content NaCl, determination of antimicrobial action by the agar well disk diffusion method using antibiotic (Amoxicillin) as a control and as indicator bacteria (Staphylococcus aureus and Escherichia coli) and isolation of genomic 16S rRNA; molecular identification. Results: Based on research results obtained five isolates of LAB, Gram staining the LAB isolated from Sui Wu’u showed that the isolated bacteria (bacilli and coccus) are Gram-positive, catalase-negative and the isolates have tolerance of viability at temperatures of 10°C, 45°C, and 50°C and to salinitas of 4% and 6.5%. The inhibitory zone LAB isolates (2PKT) against E. coli bacteria (20 mm) and S. aureus (12 mm), and (2PKB) against E. coli bacteria (17 mm) and S. aureus (10 mm). The two selected isolates were identified as Lactobacillus fermentum strain HB bacteria with 100% identification value and 98.93% query cover and L. fermentum strain HT with 100% identification value and 99.23% query cover. Conclusion: L. fermentum from Sui Wu’u has antibacterial activity against Staphylococcus aureus and Escherichia coli.

scholarly journals POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO

2015 ◽  
Vol 1 (2) ◽  
pp. 126
Author(s):  
Urnemi ◽  
Sumaryati Syukur ◽  
Endang Purwati ◽  
Sanusi Ibrahim ◽  
Jamsari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Sds Page ◽  
Inhibition Zones ◽  
And Staphylococcus Aureus

ABSTRACT Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative                                                      

scholarly journals Germicidal Activities of Some Selected Disinfectants on Staphylococcus aureus and Escherichia Coli

2020 ◽  
Vol 5 (2) ◽  
pp. 1-5
Author(s):  
Celestina MI
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Test Organisms ◽  
And Staphylococcus Aureus ◽  
Mic Value ◽  
Pathogenic Infection

The germicidal efficacy of four common disinfectants used for different purposes was tested against two common pathogens namely Escherichia coli and Staphylococcus aureus . The disinfectants (Dettol, Jik, Izal and Purit) were diluted with sterile distilled water to achieve different concentrations (100%, 50%, 25%, 12.5% and 6.25%) and they were tested on the two organisms using Agar well diffusion method. The plates were allowed to incubate for 24 hours at 37°C.The germicidal efficiency of the disinfectants was evaluated by measuring the zone of inhibition of each disinfectant based on their varying concentrations. Dettol was observed to have the highest inhibition at 100% concentration and was more germicidal on E. coli than S. aureus with Minimal Inhibitory Concentration (MIC) value of 1:16 dilution against the two test organisms. This was closely followed by Jik which also showed more inhibitory activity against E. coli at the initial concentration recording the second highest inhibition rate compared to other disinfectants, but its efficacy decreased as the concentration dropped. Jik exhibited MIC value of 1:8 and 1:2 dilutions for Staphylococcus aureus and Escherichia coli respectively. Purit showed more inhibitory activity on S. aureus with 3.8mm zone of inhibition as against 1.3mm at 100% concentration, recording MIC value of 1:8 dilution on S. aureus and 1:16 dilution for E. coli . The disinfectant Izal was observed to be the lowest as it did not inhibit the growth of any of the organisms, indicating resistance of the organisms to the disinfectant at all concentrations. All the disinfectants apart from Izal inhibited the two test isolates. Only Dettol proved to be best among them. Therefore, Dettol is encouraged to be used in homes to prevent pathogenic infection.

In vitro inhibition studies of natural resin acids to Clostridium perfringens, Staphylococcus aureus and Escherichia coli O149

2018 ◽  
Vol 6 ◽  
Author(s):  
Krisna Roy ◽  
Ulrike Lyhs ◽  
Juhani Vuorenmaa ◽  
Karl Pedersen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Clostridium Perfringens ◽  
Resin Acid ◽  
Resin Acids ◽  
Diffusion Method ◽  
Dilution Method ◽  
E Coli ◽  
Inhibition Zones

SummaryThe following experiment evaluated the inhibitory activity of a resin acids-based product (RAP) to bacterial pathogens. Clostridium perfringens isolated from chickens, turkeys and pigs, Staphylococcus aureus from chickens, pigs and cattle, and Escherichia coli O149 isolated from pigs were tested. Two different methods were used, a broth dilution method (BDM) using 0.01%, 0.1% and 0.5% resin acid, and an agar diffusion method (ADM) using 0.01%, 0.1%, 0.5%, 1% and 5% resin acid. For the BDM, C. perfringens was inhibited completely at all concentrations. S. aureus was inhibited completely at 0.5%, but only slightly at 0.1% and not at all at 0.01%. The E. coli strains showed no or little inhibition at 0.5%. For the ADM, narrow inhibition zones evolved around the concentration of 0.5% (8–10 mm), 1% (8.0–12.0 mm), and 5% (9.0–19.5 mm) on the C. perfringens strains, while the inhibition zones for S. aureus were smaller and E. coli developed no inhibition zones. Overall, the RAP inhibited C. perfringens at all concentrations of the product, S. aureus at 0.1%, 0.5%, 1% and 5% concentrations, and E. coli O149 only at 0.5% concentrations, although some strain variation was recorded.

scholarly journals Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

2014 ◽  
Vol 66 (3) ◽  
pp. 940-948 ◽  
Author(s):  
L.B. Acurcio ◽  
M.R. Souza ◽  
A.C. Nunes ◽  
D.L.S. Oliveira ◽  
S.H.C. Sandes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gastric Ph ◽  
Probiotic Potential ◽  
Sheep Milk ◽  
Potential Evaluation ◽  
And Staphylococcus Aureus

Lactic acid bacteria species were molecularly identified in milk from Lacaune, Santa Inês and crossbred sheep breeds and their in vitro probiotic potential was evaluated. The species identified were Enterococcus faecium (56.25%), E. durans (31.25%) and E. casseliflavus (12.5%). No other lactic acid bacteria species, such as lactobacilli, was identified. Most of the isolated enterococci were resistant to gastric pH (2.0) and to 0.3% oxgall. All tested enterococci were resistant to ceftazidime, oxacillin and streptomycin and sensible to clindamycin, erythromycin and penicillin. The resistance to ciprofloxacin, gentamicin, tetracycline and vancomycin varied among tested species. All tested enterococci strongly inhibited (P<0.05) Escherichia coli and Listeria monocytogenes, moderately inhibited E. faecalis and Staphylococcus aureus and did not inhibit Pseudomonas aeruginosa, Salmonella enterica var. Typhimurium and also one E. durans sample isolated from sheep milk. Four samples of E. faecium, one of E. durans and one of E. casseliflavus presented the best probiotic potential.

scholarly journals Antibacterial activity of the bacteriocins producing- lactic acid bacteria isolated from some processed meat products against selected indicator bacterial strains

2021 ◽  
Vol 12 (2) ◽  
pp. 640-645
Author(s):  
Huda M Selman ◽  
Ahmed A Mahdi ◽  
Nagat A El Rofaei ◽  
Elnasri M Mutwali
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Meat Products ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Processed Meat ◽  
Bacterial Strains ◽  
Central Public Health

In this study, a total of 25 Lactic Acid Bacteria (LAB) isolates from 15 samples [4 burgers, 4frankfurters, 3 pastramis, 2 sausages and 2baby faeces] were screened for their ability to produce inhibitory substances against three microorganisms which were isolated from meat products: (Salmonella typhi, Staphylococcus aureus and Escherichia coli). Other indicators (reference target organisms) were obtained from the Central Public Health Laboratory, Khartoum State, Sudan. These were: Salmonella typhi ATCC14023, Staphylococcus aureus ATCC29213 and Escherichia coli ATCC25922. The antagonistic activities of the isolates were screened by the direct spot-on-lawn method and well- diffusion method. A total of 25 isolates of LAB were obtained, 15 of which produced promising inhibition zones against all or some of the indicator bacterial strains.

scholarly journals POTENSI ANTIBAKTERI BINTANG LAUT DARI PERAIRAN PANTAI KELURAHAN TONGKAINA MANADO

2019 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Delpris Piter ◽  
Esther D Angkouw ◽  
Fitje Losung
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Agalactiae ◽  
Antibacterial Agents ◽  
Sea Water ◽  
Diffusion Method ◽  
E Coli ◽  
Linckia Laevigata ◽  
And Staphylococcus Aureus

Starfish is one of the species of the asteroidean class, and is grouped into phylum echinoderms. Echinoderms consist of approximately 6000 species and all live in sea water. Starfish is one source bioactive compounds. Active compounds from starfish have been known to have activities antioxidant, antibacterial, anti-inflammatory, antifungal and immunostimulatory, there are also blue starfish that are potential as antitumor and antibacterial agents. This study aims to obtain crude extracts from several types of starfish, and testing the antibacterial activity of crude starfish extracts against bacteria Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus. Test the antibacterial activity of crude starfish extracs Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata against bacteria E. coli, S. aureus, dan S. agalactiae use the agar diffusion method by means of a well.  L. l extract  can inhibit bacteria e. coli at an extract concentration of 1000 mg/ml with a diameter of inhibitory zone 11.0 mm, whereas at a concentration of 750 mg/ml the test bacteria can still grow on the bacteria S. agalactiae and S. aureus can still grow even though the extract has be given  L. l Keywords: Potential Starfish, Antibacterial Activity, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae Bintang laut merupakan salah satu spesies dari kelas Asteroidea, dan dikelompokkan kedalam filum Echinodermata. Filum Echinodermata  terdiri atas kurang lebih 6000 spesies dan semuanya hidup di air laut. Bintang laut  merupakan salah satu sumber penghasil senyawa bioaktif. Senyawa aktif dari bintang laut telah diketahui memiliki aktivitas antioksidan, antibakteri, antiinflamasi, antifungi dan imunostimulator, ada juga bintang laut biru yang potensial sebagai antitumor dan agen antibakteri. Penelitian ini bertujuan untuk mendapatkan ekstrak kasar dari beberapa jenis bintang laut, dan menguji aktivitas antibakteri ekstrak kasar bintang laut terhadap bakteri Streptococcus agalactiae, Escherichia coli dan  Staphylococcus aureus. Uji aktivitas antibakteri dari ekstrak kasar bintang laut Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata terhadap bakteri E. coli, S. aureus, dan S. agalactiae mengggunakan metode difusi agar dengan cara sumur. Ekstrak L. l mampu menghambat bakteri E.coli pada konsentrasi ekstrak 1.000 mg/ml dengan diameter zona hambat 11.0 mm, sedangkan pada konsentrasi 750 mg/ml bakteri uji masih bisa bertumbuh pada bakteri S. agalactiae dan S. aureus masih dapat bertumbuh walaupun telah diberikan ekstrak L. l.Kata Kunci : Potensi Bintang Laut, Aktivitas Antibakteri, Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus

scholarly journals The Isolation and molecular identification of antimicrobial-producing Lactic Acid Bacteria from chao, South Sulawesi (Indonesia) fermented fish product

2019 ◽  
Vol 20 (4) ◽  
pp. 1063-1068 ◽  
Author(s):  
RISKY NURHIKMAYANI ◽  
BUDI SETIADI DARYONO ◽  
ENDAH RETNANINGRUM
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Molecular Identification ◽  
Antimicrobial Compound ◽  
Fermented Fish ◽  
South Sulawesi ◽  
Fish Product

Abstract. Nurhikmayani R, Daryono BS, Retnaningrum E. 2019. Isolation and molecular identification of antimicrobial-producing Lactic Acid Bacteria from chao, South Sulawesi (Indonesia) fermented fish product. Biodiversitas 20: 1063-1068. Chao is traditional fermented fish made of anchovy (Stolephorus sp.), rice, salt, and fermented naturally on controlled environment. The product comes from Pangkep Regency, South Sulawesi. Chao has the potential for the source of high-grade lactic acid bacteria isolates, especially for antimicrobial compound. Antimicrobial compound produced by lactic acid bacteria is a biopreservative compound with many advantages. The purposes of this investigation were to isolate antimicrobial-producing lactic acid bacteria from chao and to identify their isolates based on the molecular properties. Lactic acid bacteria were isolated from chao using Man Ragosa Sharpe Agar (MRSA) medium added with 0.1 % CaCO3, followed by the ability test to produce antimicrobial compound against Staphylococcus aureus FNCC 0047 as an indicator. The spectrum of crude antimicrobial as cell-free supernatant (CFS) was determined by antagonism against S. aureus FNCC 0047 and Escherichia coli FNCC 0049 on agar well diffusion test. Selected isolates of antimicrobial-producing lactic acid bacteria molecularly identified from 16S rRNA marker using primer 27F and 1492R. We obtained four isolates of lactic acid bacteria that showed the antimicrobial activity. This crude antimicrobial compound could inhibit both gram-positive Staphylococcus aureus FNCC0047 and gram-negative bacteria Escherichia coli FNCC0049. The highest crude antimicrobial activity of isolates was observed on IB1C strain. The results of molecular characterization suggested that the IB1C and IB3B strain were identified as Lactobacillus plantarum, whereas IB3E and IB3F were identified as Pediococcus pentosaceus.

scholarly journals Antibacterial activity and toxicological evaluation of Anogeissusleiocarpus and Psidium guajava on Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
pp. 214-220
Author(s):  
S.A. Yabo ◽  
S.B. Manga ◽  
A.S. Baki ◽  
R.F. Atata ◽  
S. Gambo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Toxicological Evaluation ◽  
E Coli ◽  
Function Tests ◽  
And Staphylococcus Aureus

The study was carried out to determine the antibacterial activities and toxicological evaluation of Anogeissusleiocarpus and Psidiumguajava on Escherichia coli and Staphylococcus aureus isolated from clinical samples. The plants leaves were extracted using Hexane, Methanol, Ethanol and Water. Various concentrations (50, 25, 12.5 and 6.25mg/ml) of the crude extract of the plants were prepared and their antibacterial activity against Staphylococcus aureus and E. coli was determined using Agar well (Diffusion) method. Toxicity of the plants was evaluated, acute toxicity test, kidney and liver function tests. The result revealed that at 50mg/ml concentration, the leaf extract of Psidiumguajava was active against Staphylococcus aureus and E. coli exhibiting the highest zones of inhibition of 19mm and 9mm respectively. Whereas The leaf extract of Anogeissusleiocarpus only inhibited the growth of Staphylococcus aureus recording highest zone of inhibition of 15mm at a concentration of 50mg/ml. The plant extracts were found to be non-toxic as the LD50  was above 5000mg/kg and the biochemical parameters evaluated for both liver and kidney function tests revealed values that are within normal range. Hence the study established that consumption of the leaves of P.guajava for medicinal purpose can be said to be innocuous, as such the plant could be regarded as a potential candidate in the search of potent and harmless plants of therapeutic value.

Viabilitas Bakteri Asam Laktat dan Aktivitas Antibakteri Produk Susu Fermentasi Komersial terhadap Beberapa Bakteri Patogen Enterik

2014 ◽  
Vol 19 (1) ◽  
Author(s):  
Ekawati Purwijantiningsih
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Fermented Milk ◽  
Diffusion Method ◽  
Milk Products

AbstractFermented milk products could be found on the marketplace in great quantities of brand and type. Fermented milk has potentials for functional food because it has health benefits for human body and acts as antimicrobial. However from all brands and types, the viability of lactic acid bacteria (LAB) and antibacterial activity is not surely known, especially in Yogyakarta. The aim of this study was to determine the viability of lactic acid bacteria and antibacterial effect to three enteric pathogenic bacteria. Tests were carried out on 18 samples of fermented milk from supermarkets in Yogyakarta. Base on LAB viability test, 12 samples fulfilled the concentration of probiotic bacteria, number of viable bacteria at least 108 CFU/ml. Antibacterial activity from 18 brands against Staphylococcus aureus, Escherichia coli and Salmonella sp. by using well diffusion method. Fermented milk products have different antibacterial activities. Brand R showed the maximum antibacterial effect against the pathogens. It was determined that the most sensitive pathogenic bacteria to milk fermented products were S. aureus, whereas the least sensitive pathogen was Salmonella sp.Keywords: Antibacterial, fermented milk, viability of lactic acid bacteriaAbstrakProduk susu fermentasi dapat ditemukan di pasaran dalam berbagai merk dan jenis. Susu fermentasi tersebut berpotensi sebagai pangan fungsional karena memiliki manfaat kesehatan bagi manusia dan berperan sebagai antimikrobia. Akan tetapi dari berbagai merk dan jenis yang ada belum diketahui secara pasti mengenai viabilitas bakteri asam laktat (BAL) dan aktivitas antibakterinya terutama yang beredar di wilayah Yogyakarta. Penelitian ini bertujuan mengetahui viabilitas BAL dan efek antibakteri terhadap tiga bakteri patogen enterik. Uji dilakukan pada 18 sampel susu fermentasi yang berasal dari supermaket di kota Yogyakarta. Berdasarkan uji viabilitas BAL, ada 12 sampel yang memenuhi standar konsentrasi bakteri probiotik,paling tidak mengandung 108 CFU/ml. Aktivitas antibakteri dari 18 merk diuji terhadap Staphylococcus aureus, Escherichia coli dan Salmonella sp. menggunakan metode difusi sumuran. Produk minuman susu fermentasi memiliki kemampuan antibakteri yang berbeda. Merk R menunjukkan efek antibakteri paling tinggi terhadap beberapa bakteri patogen. Bakteri patogen yang paling sensitif terhadap produk susu fermentasi adalah S. aureus, yang paling tidak sensitif adalah Salmonella sp.Kata kunci: Antibakteri, susu fermentasi, viabilitas BAL

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