scholarly journals Antibacterial activity and toxicological evaluation of Anogeissusleiocarpus and Psidium guajava on Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
pp. 214-220
Author(s):  
S.A. Yabo ◽  
S.B. Manga ◽  
A.S. Baki ◽  
R.F. Atata ◽  
S. Gambo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Toxicological Evaluation ◽  
E Coli ◽  
Function Tests ◽  
And Staphylococcus Aureus

The study was carried out to determine the antibacterial activities and toxicological evaluation of Anogeissusleiocarpus and Psidiumguajava on Escherichia coli and Staphylococcus aureus isolated from clinical samples. The plants leaves were extracted using Hexane, Methanol, Ethanol and Water. Various concentrations (50, 25, 12.5 and 6.25mg/ml) of the crude extract of the plants were prepared and their antibacterial activity against Staphylococcus aureus and E. coli was determined using Agar well (Diffusion) method. Toxicity of the plants was evaluated, acute toxicity test, kidney and liver function tests. The result revealed that at 50mg/ml concentration, the leaf extract of Psidiumguajava was active against Staphylococcus aureus and E. coli exhibiting the highest zones of inhibition of 19mm and 9mm respectively. Whereas The leaf extract of Anogeissusleiocarpus only inhibited the growth of Staphylococcus aureus recording highest zone of inhibition of 15mm at a concentration of 50mg/ml. The plant extracts were found to be non-toxic as the LD50  was above 5000mg/kg and the biochemical parameters evaluated for both liver and kidney function tests revealed values that are within normal range. Hence the study established that consumption of the leaves of P.guajava for medicinal purpose can be said to be innocuous, as such the plant could be regarded as a potential candidate in the search of potent and harmless plants of therapeutic value.

scholarly journals POTENSI ANTIBAKTERI BINTANG LAUT DARI PERAIRAN PANTAI KELURAHAN TONGKAINA MANADO

2019 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Delpris Piter ◽  
Esther D Angkouw ◽  
Fitje Losung
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Agalactiae ◽  
Antibacterial Agents ◽  
Sea Water ◽  
Diffusion Method ◽  
E Coli ◽  
Linckia Laevigata ◽  
And Staphylococcus Aureus

Starfish is one of the species of the asteroidean class, and is grouped into phylum echinoderms. Echinoderms consist of approximately 6000 species and all live in sea water. Starfish is one source bioactive compounds. Active compounds from starfish have been known to have activities antioxidant, antibacterial, anti-inflammatory, antifungal and immunostimulatory, there are also blue starfish that are potential as antitumor and antibacterial agents. This study aims to obtain crude extracts from several types of starfish, and testing the antibacterial activity of crude starfish extracts against bacteria Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus. Test the antibacterial activity of crude starfish extracs Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata against bacteria E. coli, S. aureus, dan S. agalactiae use the agar diffusion method by means of a well.  L. l extract  can inhibit bacteria e. coli at an extract concentration of 1000 mg/ml with a diameter of inhibitory zone 11.0 mm, whereas at a concentration of 750 mg/ml the test bacteria can still grow on the bacteria S. agalactiae and S. aureus can still grow even though the extract has be given  L. l Keywords: Potential Starfish, Antibacterial Activity, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae Bintang laut merupakan salah satu spesies dari kelas Asteroidea, dan dikelompokkan kedalam filum Echinodermata. Filum Echinodermata  terdiri atas kurang lebih 6000 spesies dan semuanya hidup di air laut. Bintang laut  merupakan salah satu sumber penghasil senyawa bioaktif. Senyawa aktif dari bintang laut telah diketahui memiliki aktivitas antioksidan, antibakteri, antiinflamasi, antifungi dan imunostimulator, ada juga bintang laut biru yang potensial sebagai antitumor dan agen antibakteri. Penelitian ini bertujuan untuk mendapatkan ekstrak kasar dari beberapa jenis bintang laut, dan menguji aktivitas antibakteri ekstrak kasar bintang laut terhadap bakteri Streptococcus agalactiae, Escherichia coli dan  Staphylococcus aureus. Uji aktivitas antibakteri dari ekstrak kasar bintang laut Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata terhadap bakteri E. coli, S. aureus, dan S. agalactiae mengggunakan metode difusi agar dengan cara sumur. Ekstrak L. l mampu menghambat bakteri E.coli pada konsentrasi ekstrak 1.000 mg/ml dengan diameter zona hambat 11.0 mm, sedangkan pada konsentrasi 750 mg/ml bakteri uji masih bisa bertumbuh pada bakteri S. agalactiae dan S. aureus masih dapat bertumbuh walaupun telah diberikan ekstrak L. l.Kata Kunci : Potensi Bintang Laut, Aktivitas Antibakteri, Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus

scholarly journals Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Megawati S. Saroinsong ◽  
Febby E. F. Kandou ◽  
Adelfia Papu ◽  
Marina F. O. Singkoh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Activity Test ◽  
And Staphylococcus Aureus ◽  
Paper Disc ◽  
Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals Antibacterial activity of different extract of medicinal plant Antirrihnum Orontium.

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Shehzad Saleem ◽  
Wisal Ahmad ◽  
Muhammad Hanif ◽  
Zahid Ali ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Water Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Water Extracts ◽  
The Family ◽  
Quaternary Base ◽  
And Staphylococcus Aureus

Antirrhinum Orontium is belonging to the family Plantaginacea commonly known as lesser snapdragon which is a flowering plant belong to genus Antirrhinum. It was traditionally used in treatment of Tumor, Eye inflammation and Ulcer. Antirrhinum Orontium is contain mostly tertiary alkaloids bases one of them is identified as 4-methyl-2, 6-naphthyridine and Choline is identified as quaternary base. The recent study is to investigate the antibacterial activity of Ethyl acetate, Dichloromethane, Hexane and Water extracts of Antirrhinum Orontium, against the pathogen like Escherichia coli and Staphylococcus aureus. Both the Soxhlet and Maceration water extract were found most active from the other extract against Escherichia coli and Staphylococcus aureus by using agar well diffusion method. The measured zone of inhibition for Soxhlet water extract is 21±0.05mm and Maceration water extract is 20±0.05mm against gram positive Staphylococcus aureus, while for gram negative Escherichia coli 23±0.05mm is measured for Soxlet and 21±0.05mm measured for Maceration water extracts

scholarly journals Hand Sanitizer Ekstrak Daun Pucuk Idat (Cratoxylum glaucum) sebagai Antibakteri Staphylococcus aureus dan Escherichia coli

2020 ◽  
Vol 16 (2) ◽  
pp. 227
Author(s):  
Megawati Ayu Putri ◽  
Marhan Ebit Saputra ◽  
Ike Nur Amanah ◽  
Siska Musiam ◽  
Verry Andre Fabiani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Irritation ◽  
Test Results ◽  
Antibacterial Test ◽  
E Coli ◽  
Hand Sanitizer ◽  
Carboxymethyl Cellulose Sodium

<p><span id="docs-internal-guid-2cebd816-7fff-d997-4dfd-796d046d0d3b"><span>Daun pucuk idat merupakan tanaman lokal Bangka Belitung yang memiliki aktivitas antibakteri terhadap </span><span>Staphylococcus aureus </span><span>dan</span><span> Escherichia coli.</span><span>Sebagai upaya pemanfaatan potensi lokal maka dibuat suatu produk sediaan gel </span><span>hand sanitizer</span><span> yang praktis dan ekonomis. </span><span>Ekstrak daun pucuk idat diperoleh dengan metode maserasi menggunakan pelarut aseton. Ekstrak yang dihasilkan diformulasikan dalam berbagai konsentrasi ekstrak daun pucuk idat (40%, 60%, 80%, dan 100%) dengan penambahan </span><span>carboxymethyl cellulose sodium</span><span> (</span><span>CMC-Na) 0,25 g, gliserin 1 mL, propilenglikol 0,5 mL serta akuades        10 mL. Setiap formulasi gel </span><span>handsanitizer</span><span> dilakukan pengujian stabilitas fisik meliputi uji organoleptis, uji homogenitas, uji daya sebar, uji pH, uji iritasi kulit serta uji aktivitas antibakteri.</span><span> </span><span> Hasil uji organoleptik menunjukkan bahwa semua konsentrasi </span><span>hand sanitizer </span><span>berbentuk gel, berwarna kuning muda, berbau dan homogen. Uji pH dan daya sebar gel</span><span> hand sanitizer</span><span> telah memenuhi ketentuan SNI No. 06-2588. Pengujian iritasi kulit tidak menimbulkan kemerahan, gatal-gatal dan tidak mengakibatkan kulit menjadi kasar pada punggung tangan sukarelawan. Berdasarkan uji sifat fisik gel</span><span> hand sanitizer </span><span>yang dihasilkan memiliki sifat fisik yang serupa dengan gel </span><span>hand sanitizer </span><span>komersial. Hasil uji antibakteri hand sanitizer ekstrak daun pucuk idat terhadap </span><span>E. coli</span><span> dengan konsentrasi ekstrak daun pucuk idat 40%, 60%, 80%, dan 100% diperoleh diameter zona hambat berturut-turut sebesar 11,5 mm, 17,4 mm, 19,3 mm dan 19,3 mm, sedangkan pada bakteri </span><span>S. aureus</span><span> diperoleh diameter zona hambat berturut-turut sebesar 17,1 mm, 24,2 mm, 27,4 mm, dan  18 mm. Hasil uji antibakteri menunjukkan bahwa sediaan gel </span><span>hand sanitizer </span><span>ekstrak aseton daun pucuk idat memiliki aktivitas antibakteri terhadap bakteri </span><span>E. coli </span><span>dan </span><span>S. aureus</span><span>.</span></span></p><p><span><span><span id="docs-internal-guid-50d6ff2c-7fff-2e66-45f1-73cf49e6527a"><strong>Hand Sanitizer of Leaves Extract of Cratoxylum glaucum as Antibacterial of Staphylococcus aureus and Escherichia coli. </strong><span>Cratoxylum glaucum</span><span> leaves is local plants of Bangka Belitung which have antibacterial activity against </span><span>S. aureus</span><span> and </span><span>E. coli</span><span>. As an effort to explore local potential, a practical and economical gel hand sanitizer product was prepared. </span><span>Cratoxylum glaucum</span><span> leaves extract was obtained by maceration method using acetone. The extract was formulated in various concentrations of </span><span>Cratoxylum glaucum</span><span> leaf extract (40%, 60%, 80%, and 100%) with the addition of 0.25 g carboxymethyl cellulose sodium (CMC-Na), 1 mL glycerin, 0.5 mL propylene glycol and 10 mL distilled water. Each concentration of the hand sanitizer gel was tested for physical stability including organoleptic, homogenity, spreadability, pH, skin irritation and antibacterial activity test. The organoleptic test results showed that all concentrations of hand sanitizers formed gel with light yellow color, smelly odor and homogeneous form. The analysis of pH and spreadability of hand sanitizer gel has fulfilled SNI No. 06-2588. The skin irritation testing does not cause redness, itching, and rough skin on the back of volunteer hands. Based on the physical properties test, the resulting hand sanitizer gel has physical properties similar to commercial hand sanitizer gel. The results of the antibacterial test of hand sanitizer against </span><span>E. coli</span><span> with </span><span>Cratoxylum glaucum</span><span> leaf extract concentrations of 40%, 60%, 80%, and 100% obtained the diameter inhibitory zones of 11.5 mm, 17.4 mm, 19.3 mm, and 19.3 mm, respectively. Meanwhile, the results of the antibacterial test of hand sanitizer against </span><span>S. aureus</span><span> bacteria with concentration of 40%, 60%, 80%, and 100% shows that the inhibition zone diameters were 17.1 mm, 24.2 mm, 27.4 mm, and 18 mm, respectively. The antibacterial test results shows that the gel hand sanitizer prepared has antibacterial activity against </span><span>E. coli</span><span> and </span><span>S. aureus</span><span> bacteria.</span></span></span></span></p>

scholarly journals The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria

2018 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Abrianto A. O. Rompis ◽  
Fitje Losung ◽  
Deiske A. Sumilat ◽  
Agung B. Windarto ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Compounds ◽  
E Coli ◽  
Escherichia Coli Bacteria ◽  
And Staphylococcus Aureus ◽  
Positive Results ◽  
Sponge Extract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm).  Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu:  ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (COFFEA CANEPHORA L.) LEAVES TO STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2019 ◽  
pp. 113-115
Author(s):  
ZAMHARIRA MUSLIM ◽  
YONANIKO DEPHINTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Effect ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Robusta Coffee

Objective: This research aims to analyze the ability of robusta coffee leaves fraction extract to inhibit the growth of Staphylococcus aureus and Escherichia coli and also determine the minimum inhibitory concentration (MIC). Methods: Antibacterial activity evaluated by the disc diffusion method observed in four types of fraction of extract robusta coffee leaves (n-hexane, ethyl acetate, ethanol, and water). Each extract divided into three various concentrations, 5%, 10%, and 15%. Determination of antimicrobial activity in vitro by the disk diffusion method. Results: Ethyl acetate fraction of coffee leaves extract produced the largest diameter zone of inhibition of bacterial growth compared to other extraction fractions of 17.28 mm in E. coli and 18.58 mm in S. aureus. The MIC of coffee leaves extract fraction water, ethyl acetate, and n-hexane on E. coli and S. aureus is 5%, while the fraction ethanol MIC is 10%. Conclusion: The antibacterial effect of ethyl acetate fraction of coffee leaves extract showed an antibacterial effect that was better than the fraction of n-hexane, ethanol, and water.

scholarly journals ANTIBACTERIAL ACTIVITY OF Samanea saman LEAF ETHANOL EXTRACT AGAINST Escherichia coli AND Staphylococcus aureus AND ITS TOTAL FLAVONOID AND PHENOLIC CONTENTS

Jurnal Kimia ◽  
2018 ◽  
pp. 121
Author(s):  
W. S. Rita ◽  
I M. D. Swantara ◽  
I. A. R. Astiti Asih ◽  
N.K. Sinarsih
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
E Coli ◽  
Phenolic Contents ◽  
Agar Disc ◽  
Samanea Saman ◽  
And Staphylococcus Aureus

Antibacterial activity of Samanea saman usually shows a positif correlation to the flavonoid and phenolic contents.  The aim of this study was to evaluate the antibacterial activity of the ethanol extract of Samanea saman against Escherechia coli and Staphylococcus aureus and determine the total flavonoid and phenolic contents of the extract. The extraction was done by ethanol 96% at room temperature. The antibacterial assay was conducted by agar disc diffusion method. The total flavonoid and phenolic contents were determined by UV-Vis Spectrofotometer with the standard of quersetin and galic acid, respectively. The extraction of 250 g of Samanea saman leaves resulted in 24.5 g of ethanol extracts. The ethanol extract showed a moderate inhibition of 8.33 mm towards E. coli and a strong inhibition of 13.6 mm towards S. aureus at the concentration of 4%. The Minimum Inhibitory Concentration (MIC) of the extract against E. coli and S.aureus were of 3% and 0.3%”, respectively. The total flavonoid and phenolic contents were successively 1233.2991 mg QE/100g and 2544.6154 mg GAE/100g. Keywords: Escherechia coli, flavonoid and phenolic content,  Samanea saman, Staphylococcus aureus

scholarly journals Hydrothermal Fabrication of Spindle-Shaped ZnO/Palygorskite Nanocomposites Using Nonionic Surfactant for Enhancement of Antibacterial Activity

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1453 ◽  
Author(s):  
Aiping Hui ◽  
Shuqing Dong ◽  
Yuru Kang ◽  
Yanmin Zhou ◽  
Aiqin Wang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Ionic Surfactants ◽  
E Coli ◽  
Antibacterial Performance ◽  
Good Antibacterial Activity ◽  
Controllable Growth ◽  
And Staphylococcus Aureus

In order to improve the antibacterial performance of natural palygorskite, spindle-like ZnO/palygorskite (ZnO/PAL) nanocomposites with controllable growth of ZnO on the surface of PAL were prepared in the presence of non-ionic surfactants using an easy-to-operate hydrothermal method. The obtained ZnO/PAL nanocomposites have a novel and special spindle-shaped structure and good antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and are also low cost. The minimum inhibitory concentrations of ZnO/PAL nanocomposites toward E. coli and S. aureus reached 1.5 and 5 mg/mL, respectively.

Bakteri Simbion Karang Porites dari Perairan Gunungkidul, Yogyakarta dan Aktivitas Antibakteri terhadap Bakteri Patogen Staphylococcus aureus dan Escherichia coli

2018 ◽  
Vol 7 (1) ◽  
pp. 43
Author(s):  
Rivan Novianto Madilana ◽  
Diah Permata Wijayanti ◽  
Agus Sabdono
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
16S Rrna ◽  
Bacillus Pumilus ◽  
Coral Tissue ◽  
Diffusion Method ◽  
Rrna Gene ◽  
E Coli ◽  
Porites Coral

Porites merupakan genus karang yang memiliki persebaran luas di Indonesia, termasuk perairan Gunungkidul, Yogyakarta. Penelitian menunjukkan bahwa bakteri simbion karang Porites memiliki potensi antibakteri dalam menanggulangi bakteri patogen. Penelitian ini betujuan untuk mengetahui jenis bakteri simbion karang Porites dari Perairan Gunungkidul Yogyakarta yang memiliki aktivitas antibakteri patogen S. aureus dan E. coli. Bakteri simbion diisolasi dari fragmen jaringan karang dengan pengenceran bertingkat, kemudian uji aktivitas antibakteri dilakukan dengan menggunakan metode overlay dan difusi paperdisk. Delapan dari 64 isolat aktif menghambat kedua bakteri patogen Staphylococcus aureus dan Escherichia coli. Dua diantaranya merupakan isolat unggul yang menunjukkan aktivitas antibakteri paling baik. Kedua isolat selanjutnya diidentifikasi karakteristik molekular DNA dengan sekuen gen 16S rRNA. Hasil identifikasi 16S rRNA menunjukkan isolat GKP1.4.3 memiliki kesamaan 99% dengan Bacillus pumilus strain NBRC 12092, dan isolat GKP3.2.2 memiliki kesamaan 99% dengan Vibrio natriegens strain NBRC 15636.Porites is a coral which has distributed widely in Indonesia, including Gunungkidul Waters, Yogyakarta. Research has shown that Porites coral symbiont bacteria have antibacterial potency against pathogenic bacteria.This study aims to determine the type of Porites coral symbiont bacteria collected from the waters of Gunungkidul Yogyakarta which has antibacterial activity of S. aureus and E. coli. Bacteria symbionts were isolated from coral tissue fragments by serial dillution method, while antibacterial activity was performed by using overlay and paperdisk diffusion method. Eight of the 64 active isolates inhibited both pathogenic bacteria S. aureus and E. coli. Two of 8 isolates showed stronger antibacterial activity. The two isolates subsequently identified the molecular characteristics of DNA with the 16S rRNA gene sequence. The identification of 16S rRNA showed that GKP1.4.3 isolate had 99% similarity with Bacillus pumilus strain NBRC 12092, and GKP3.2.2 isolate had 99% similarity with Vibrio natriegens strain NBRC 15636.

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