scholarly journals Bacillus subtilis from Potato Rhizosphere as Biological Control Agent and Chili Growth Promoter

2019 ◽  
Vol 23 (2) ◽  
pp. 179
Author(s):  
Nur Prihatiningsih ◽  
Heru Adi Djatmiko ◽  
Erminawati Erminawati ◽  
Puji Lestari
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Pathogens ◽  
Biological Control Agent ◽  
Plant Growth Promoting Rhizobacteria ◽  
Dry Mass ◽  
Growth Promoter ◽  
Soluble Phosphate ◽  
Root Mass ◽  
Colletotrichum Spp

Bacillus subtilis is an antagonist bacteria  that inhibits the growth of fungal and bacterial plant pathogens. The B. subtilis has roles as biocontrol agents and plant growth promoting rhizobacteria (PGPR). This research aimed to evaluate the potency of B. subtilis isolates (B209, B211, and B298) as a biocontrol agent to anthracnose (caused by Colletotrichum spp.) and as PGPR to increase the growth of chili plants. The experiments were divided into two batches. The first batch was conducted in the laboratory to evaluate the characteristics of B. subtilis (as biocontrol) and PGPR (phosphate solubility, producing IAA and nitrogen). The second batch was conducted in the field in Rempoah Village, Baturraden Regency, Banyumas District, with 5 treatments and 6 replications. Parameters observed were inhibition percentage to Colletotrichum spp., disease intensity, the component of PGPR, plant growth of chili, and phatosystem. The results showed that B209, B211, and B298 isolates inhibited the growth of Colletotrichum spp, with the highest inhibition percentage on B298. B209, B211, and B298 have characteristics as PGPR, i.e. the ability to soluble phosphate, to produce IAA and nitrogen. The ability of B298 to promote plant growth was shown by the increase of plant height, leaf number, plant dry mass, and dry root mass (38.0%, 54.7%, 61.7%, 61.8%, respectively). B298 and B211 could increase the fresh crop mass (41.2% and 37.1%) and fresh root mass (36.4% and 34.4%). B298 and B209 were similar in increasing the root length (25.2%). Root volume could be increased by 33.3% by applying B211 isolate. B209 was the best isolate to reduce anthracnose up to 80.36%.

scholarly journals A Comprehensive Study on IAA production by Bradyrhizobium japonicum and Bacillus subtilis and Its Effect on Vigna radiata Plant Growth

2020 ◽  
Author(s):  
S. Kiruthika ◽  
M. Arunkumar
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Vigna Radiata ◽  
Bradyrhizobium Japonicum ◽  
Critical Role ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth Promoter ◽  
Iaa Production ◽  
Root And Shoot Length

Background: The use of chemical fertilizers and pesticides raises concerns about environmental pollution, health hazards and the destruction of biotic groups that support plant growth. Plant growth-promoting rhizobacteria (PGPR) thrive in the rhizosphere of plants are the auspicious alternative for these chemicals. PGPR plays a critical role in plant growth and development, along with biocontrol activities. Methods: In this present study, two effective microbes, Bradyrhizobium japonicum and Bacillus subtilis were chosen and their ability to produce Indole Acetic acid (IAA) was determined. Optimization of IAA production was carried out in different cultural conditions. Further, in-vitro studies were carried out to analyze the effect of these bacteria on the growth of Vigna radiata. Results: Our investigations showed that both organisms have the potential to produce IAA under standard conditions. IAA production is maximum when using Bradyrhizobium japonicum with the supplement of Carboxymethyl cellulose and yeast extract as C and N source, respectively. L-Tryptophan concentration has a positive effect on production. Further, the application of bacterial cultures has shown more significant improvement in plant growth in terms of root and shoot length and weight of crop material. The current findings recommend that Bradyrhizobium japonicum can be a suitable organism for application as a plant growth promoter.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

scholarly journals Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512
Author(s):  
Nanda Dwi Hafri ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Allium Cepa ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

scholarly journals Importance of plant growth promoting rhizobacteria (PGPR) in agriculture: A Review

2020 ◽  
Vol 35 (1-2) ◽  
Author(s):  
Tabish Akhtar ◽  
Shubham Kumar ◽  
Sukhdeo Kumar ◽  
M. R. Meena
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Plant Pathogens ◽  
Crop Yields ◽  
Plant Growth Promoting Rhizobacteria ◽  
Agricultural Industry ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Increasing Demand ◽  
Environmentally Sound

The growth of plants promoting rhizobacteria (PGPR) has gained widespread importance in agriculture. These are beneficial bacteria found in nature that live actively in plant roots and improve plant growth and increase agricultural productivity.. (PGPR) promoting plant growth shows an important role in the sustainable agricultural industry. The increasing demand for crop production is a major challenge nowadays, with a significant lack of use of synthetic chemical fertilizers and pesticides. The use of PGPR has proven to be an environmentally sound way of increasing crop yields by facilitating plant growth through direct or indirect mechanisms. The mechanisms of PGPR include regulating hormonal and nutritional balance, inducing resistance against plant pathogens, and dissolving nutrients for easy uptake by plants. Furthermore, PGPRs show synergistic and antagonistic interactions with microorganisms within the rhizosphere and in bulk soils, which indirectly increases plant growth rates. There are several bacteria species that act as PGPR. This review summarizes the methodology of PGPR as a bio-fertilizer in agriculture.

scholarly journals Influence of Plant growth promoting Rhizobacteria on growth and Biochemical Parameters of Corn (Zea mays l.) Var. Seedlings

2020 ◽  
Vol 1 (3) ◽  
pp. 9-24
Author(s):  
Huma Nawaz ◽  
Saima Javed ◽  
Muhammad Faisal
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Biochemical Parameters ◽  
Agricultural Practices ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Maximum Increase ◽  
Pot Trials ◽  
Plant Growth Promoting ◽  
Bacillus Strains

Impact of inoculations of eight individual strains (CN2, CSH4, Cu35, CMS7, CSH23, CSH27, CF18, and Cu47, two combinations (Comb 4A and Comb 4B) of four Bacillus strains and mixture of all strains (Mix) was studied. Various growth and biochemical parameters of maize plant were determined in pot trials. Strain Bacillus subtilis CN2 showed 49% maximum increase in shoot length. The blend of entire strains was observed to have their lenghth increased about 21 %. Bacillus subtilis CN2 showed maximum increase 155%, in fresh biomass and 233 % increase in dry weight. Bacillus amyloliquefaciens (Cu47) and Comb 4A and showed maximum peroxidase content of 163 % and 94 % as compared to uninoculated control. Comb 4B was shown to have significant higher content of acidphosphatase (811 %). Increase in all other physical and biochemical parameters were noticed. Therefore, Bacillus strains exhibited characteristic increased potential of plant growth and can have great application in innovative agricultural practices.

scholarly journals Efektivitas Formulasi Spora Bacillus subtilis B12 sebagai Agen Pengendali Hayati Penyakit Hawar Daun Bakteri pada Tanaman Padi

2015 ◽  
Vol 34 (1) ◽  
pp. 21 ◽  
Author(s):  
Wartono Wartono ◽  
Giyanto Giyanto ◽  
Kikin H. Mutaqin
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Foliar Spray ◽  
Leaf Blight ◽  
Plant Diseases ◽  
Bacterial Leaf Blight ◽  
Growth Promoter ◽  
Negative Effect ◽  
Plant Growth Promoting ◽  
Blight Disease

<p>Bacterial leaf blight control in rice (Oryza sativa Lin.) using bactericide is prohibitive, due to its high cost and its negative effect on the environment. Biocontrol when avaible, therefore is the best alternative solution. Bacillus subtilis is a perspective of biocontrol agent to control several plant diseases, because of its ability to produce antimicrobial and produce plant growth promoting substances. This research was aimed to examine the effectiveness of B. subtilis spore formulation by way of seed treatments and foliar sprays, using different frequencies and concentrations, to control bacterial leaf blight disease (BLB) on rice, caused by Xanthomonas oryzae pv. oryzae, and to evaluate its function as plant growth promoter. The experiments were conducted at greenhouse and in the field using factorial design. At the greenhouse experiment, seed treatment and foliar spray, using concentration of 2% produced better result in controlling BLB, and better result on promoting rice plant growth. In the field experiment, application at 2 week interval showed better effect on suppressing the the disease and on increasing yield. Applications of the formulation of B. subtilis B12 spore reduced BLB disease by 21% and potentially increased yield up to 50%.</p>

Plant growth promoting rhizobacteria (PGPR): Biofertiliser and Biocontrol agent-Review article

2019 ◽  
Vol 8 ◽  
pp. 42-45
Author(s):  
Anup Muni Bajracharya
Keyword(s):  
Plant Growth ◽  
Plant Pathogens ◽  
Agricultural Soils ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Productivity ◽  
Good Health ◽  
Systemic Resistance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Good Food

Good health starts with good food. Humans expect agriculture to supply good food with sufficient nutrients, economically and culturally valued foods, fibers and other products. But the excessive application of synthetic pesticides has exerted an adverse effect on bio-flora, fauna and natural enemies. Even a largest part of yield has been lost due to various stresses, like biotic and abiotic stresses to the plant. On this account, plant growth promoting rhizobacteria (PGPR), an eco-friendly biopesticides is boon for the biocontrol of different plant pathogens. Moreover, PGPR strains can enhance the plant growth through the production of various plant growth promoting substances. These are generally a group of microorganism that is found either in the plane of the rhizosphere or above roots impacting some positive benefits to plants. PGPR are associated with plant roots and augment plant productivity and immunity; however, recent work by several groups shows that PGPR also elicit so-called 'induced systemic tolerance' to salt and drought. PGPR might also increase nutrient uptake from soils, thus reducing the need for fertilizers and preventing the accumulation of nitrates and phosphates in agricultural soils. Scientific researches involve multidisciplinary approaches to understand adaptation of PGPR, effects on plant physiology and growth, induced systemic resistance, biocontrol of plant pathogens, bio fertilization, and potential green alternative for plant productivity, viability of co inoculating, plant microorganism interactions, and mechanisms of root colonization.

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