scholarly journals The Role of Sechium edule Fruits Ethanolic Extract in Insulin Production and Malondialdehyde Level in Stz-Induced Diabetic Rat

2019 ◽  
Vol 4 (1) ◽  
pp. 11
Author(s):  
Betty Lukiati ◽  
Nugrahaningsih Nugrahaningsih ◽  
Siti Nur Arifah
Keyword(s):  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Sechium Edule ◽  
Liver And Pancreas ◽  
Group A ◽  
Kidney Liver

The objectives of this research were to examine the effect of Sechium edule ethanolic extract in insulin and Malondialdehyde (MDA) levels in streptozotocin (STZ) induced diabetic rats. Type 1 diabetic rat were obtained by intraperitonially injected with multiple low dose STZ (MLD-STZ) 20 mg/kgBW for 5 days. The animals were divided into 5 groups: untreated rats in group (K) were considered as negative control, MLD-STZ induced type 1 diabetic rats in group (S) were considered as positive control. In another 3 group (A, B, C) type 1 diabetic rats were orally treated with three doses of S. edule ethanolic extract (14, 28, 42 mg/kg BW) for 7 days. The animals were sacrificed in two days after last treatment, serum were collected for measuring of the insulin concentration by ELISA method. Level of MDA on kidney, liver and pancreas were measured by TBA method. The treatment with S. edule ethanolic extracts unable to increase of insulin yet but the treatment with 42 mg/kg BW of S. edule ethanolic extract showed significantly decreased MDA levels on liver but not significant on kidney and pancreas.

scholarly journals The Potenty of Sechium edule Ethanolic Extract to Repair Beta Pancreas Cells and Nitrogen Oxide Concentration in Streptozotocin-induced Diabetic Rat

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Betty Lukiati ◽  
Siti Imroatul Maslikah ◽  
Nugrahaningsih N
Keyword(s):  
Nitrogen Oxide ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Type I ◽  
Sechium Edule ◽  
Group 2

The objectives of this research were to examine the effect of Sechium edule ethanolic extract to repair beta pancreas cells damage and tomeasure nitrogen oxide (NO) concentration in streptozotocin-induced rats. Type I diabetic rats were obtained by intraperioneally injected therats with multiple low dose streptozotocin (MLD-STZ) for 5 days. In this research, rats were assigned into 5 treatment groups: untreated rats ingroup 1 were considered as negative control, while MLD-STZ induced type 1 diabetic rats in group 2 were considered as positive control. Inanother 3 groups, type 1 diabetic rats were orally treated with three doses of Sechium edule ethanolic extract (14, 28, 42 mg/kg bw) respectivelyfor 7 consecutive days. Repairing of beta pancreas cells were analyzed descriptively whereas concentration of NO were analyzed using one wayANOVA and continued with LSD test (α= 0.01). The result showed that the administration of Sechium edule ethanolic extract significantlydecreased NO concentration (P<0.01) up to 67.9 µM and repaired beta pancreas cells damage. Dose of 42 mg/kg bw of Sechium edule ethanolicextract is the effective dose for diabetic rat therapy. Key words: Sechium edule extract, diabetic mellitus, rat, beta pancreas cells

scholarly journals Hypoglycaemic Property of Nutraceuticals

1970 ◽  
Vol 6 (2) ◽  
pp. 105-107
Author(s):  
Mohammad Emdadul Haque ◽  
Quazi Safayetullah ◽  
Monira Ahsan ◽  
Sheikh Nazrul Islam
Keyword(s):  
Glucose Level ◽  
Day Treatment ◽  
Positive Control ◽  
Serum Glucose ◽  
Negative Control ◽  
Diabetic Rats ◽  
Serum Glucose Level ◽  
Diabetic Rat ◽  
Black Cumin ◽  
Control Serum

Hypoglycemic property of four nutraceuticals named - black cumin, garlic, methi and karela has been investigated on an alloxan induced diabetic rat model. Glibenclamide was used as positive control and normal saline was used as negative control. Serum glucose levels of normal and diabetic induced rats were estimated by Glucose Oxidase method using a kit, and it were 4.33 ± 0.43 mmol/L and 8.27 ± 0.45mmol/L respectively. The alloxan induced diabetic rats were grouped into six, which were then treated with the four nutraceuticals and two controls. Treatment was given orally everyday for 28 days. Serum glucose level was analysed every 7 day intervals. It was observed that lowering of glucose level was significant (P = 0.01) for every 7 day treatment period. All of the nutraceuticals reduced the serum glucose level nearly to the normal value within 28 days. The rate of lowering of the glucose level was found to vary with the nutraceuticals. Garlic was found to be most active like the glibenclamide. The next active one was black cumin followed by methi and karela. The study conclude that nutraceuticals possess significant hypoglycemic property. Key words: Hypoglycaemia; Nutraceuticals; Black cumin; Garlic; Methi; Karela. Dhaka Univ. J. Pharm. Sci. 6(2): 105-107, 2007 (December)

scholarly journals Effects of Extracts of Plathymenia Reticulata Benth and Azadirachta Indica (Neem) in Glycated Hemoglobin in Diabetic Rats

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A438-A439
Author(s):  
Ezio De Martino Neto ◽  
Joyce Satil Chaves da Silva ◽  
Eliane Cristina Lourenço ◽  
Arthur Cesário de Castro Neto ◽  
Isabella Cecilio Resende Ferreira ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Azadirachta Indica ◽  
Insulin Treatment ◽  
Glycated Hemoglobin ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Male Adult ◽  
Significant Difference

Abstract Introduction: The Plathymenia reticulata benth is a herbal medicine that has properties of pancreatic islet hyperplasia and glycemic control in diabetic rats. Neem (Azadirachta indica A. Juss, Meliaceae) is a tree native to India that has several medicinal effects. Goal: To verify the effect of glycated hemoglobin levels in rats with type 1 and non-diabetic diabetes mellitus, in treatment with Plathymenia Reticulata Benth, Neem and the association between them. compared to insulin. Methodology: Diabetes was induced by intraperitoneal streptozotocin (65mg/kg) administration after a 24-hour fast. The diagnosis was made using a blood glucose value above 200mg/dl. The study was conducted in 60 male adult Wistar rats, weighing between 180 and 220 grams, divided into 9 groups, between diabetics (DM) and non-diabetic controls (NdM), and treated with Neem (300 mg/kg), cold aqueous extract of Plathymenia (100 mg/kg), water (negative control) and insulin (3 IU/day) - positive control; and association between plants. The treatment was performed by orogastric gavage for a period of 28 consecutive days, and weekly weight and daily feed intake were performed. Data were analyzed using ANOVA and Tukey-Kramer’s pos-hoc test, with a significance level of 5% using the SPSS25.0 software. The results are expressed on average ± EPM. Results: There was a significant difference in glycated hemoglobin levels in rats submitted to insulin treatment (6.18 ± 0.36) compared to those submitted to treatment with Neem (10.12 ± 1.29, p=0.047), Plathymenia+Neem (12.09 ± 0.38, p=0.006) and water (10.86 ± 1.26, p=0.015). However, no significant difference was observed between the reduction in glycated hemoglobin levels in the groups submitted to insulin treatment compared to the group treated with Plathymenia (7.30 ± 0.68, p=0.911). Conclusion: The results allow us to evaluate a non-inferiority condition in relation to the use of the Plathymenia when compared to treatment with insulin therapy, positive control in the treatment of type 1 diabetes mellitus. The Plathymeniamay present as a herbal option in the treatment of the disease and prevention of complications. Further studies are necessary to evaluate the effect of the extract on other aspects related to the pathology.

scholarly journals The Assay of Blood Plasma’s Malondialdehyde (MDA) Activity in Alloxan-Induced Diabetic Rat Given Yellow Velvet Leaf Extract (Limnocharis flava)

2020 ◽  
Vol 18 (2) ◽  
pp. 157-162
Author(s):  
Yithro Serang ◽  
Ainun Nur Hammi
Keyword(s):  
Ethanolic Extract ◽  
Negative Control ◽  
Diabetic Rats ◽  
The Body ◽  
Diabetic Rat ◽  
Thiobarbituric Acid Reactive Substance ◽  
Alternative Source ◽  
White Rats ◽  
Significant Difference ◽  
One Way Anova

It has been well-known that Genjer or yellow velvet leaf (Limnocharis flava) has a potential as an alternative source to natural antioxidants. Antioxidants, such as flavonoids, alkaloids, phenols, tannins, and others are substances which can block the presence of free radicals coming into the body. It has also been examined to be beneficial in treating metabolic syndrome diseases such as diabetes, as it improves insulin’s performance. The objective of this study is to examine the antioxidant effect of yellow velvet leaf’s ethanolic extract (Limnocharis Flava) on blood plasma’s MDA activity in alloxan-induced diabetic rats (Rattus novergicus) in three different doses. The testing animals were randomly divided into five groups, each group consisted of 6 white rats. Group 1 was the negative control and given CMC Na 0.5%, while group 2 was a positive control and given glibenclamide 0.45 mg, group 3 was yellow velvet leaf’s ethanolic extract of 32.5 mg/kg, group 4 was yellow velvet leaf’s ethanolic extract of 65 mg/kg, group 5 was yellow velvet leaf’s ethanolic extract of 130 mg/kg. Previously, all groups were induced with alloxan through intra peritoneal injection. Ethanol extract was provided once on the day of 7th, 14th, and 21st. The observed parameters were blood plasma’s MDA activity in the alloxan-induced diabetic rats (Rattus novergicus). Blood plasma’s MDA activities were assessed by using Thiobarbituric Acid Reactive Substance (TBARs) method. Then, data were collected and analyzed by using One Way ANOVA followed by a Post hoc test. The results showed that the mean values of MDA levels in the testing groups of 1,2,3,4 and 5 were 9.30 ± 0.462, 2.17 ± 0.121, 6.11 ± 0.381, 4.07 ± 0.327, and 2.75 ± 0.121, respectively. One Way ANOVA test showed a significant difference in the blood plasma’s MDA levels among the groups (p = 0,000). It is concluded that the blood plasma’s MDA levels in alloxan-induced diabetic rats can be lowered by the yellow velvet leaf’s ethanolic extract (Limnocharis Flava). Therefore, it can be employed as a traditional treatment for diabetes.

scholarly journals Histopathological Changes of Egg Cells in the Uterine of Ascaridia galli after Treatment with Extract of Veitchia merrillii Nuts

2017 ◽  
Vol 22 (3) ◽  
pp. 139
Author(s):  
Ummu Balqis ◽  
Muslina Muslina ◽  
Muhammad Hambal ◽  
Farida Athaillah ◽  
Muttaqien Muttaqien ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ascaridia Galli ◽  
Histopathological Changes ◽  
Intestinal Nematode ◽  
Reproduction Process ◽  
Group A ◽  
Phosphate Buffered Saline

The aim of the study was to observe the histopathological changes of the entire egg cells in the uterine of Ascaridia galli worms treated with crude ethanolic extract of Veitchia merrillii nuts. Histopathological changes of the entire egg cells were observed to describe that the activity of extract V. merrillii nuts interfered the reproduction process could decrease the quantity and quality of A. galli eggs. The experiments consisted of 16 head A. galli female adult worms divided into four groups, contained four worms of each, and maintained in 0.9% phosphate buffered saline (PBS). First group, a negative control experiment consisted of A. galli in 0.9% PBS. Second group, concentration of 15 mg/mL albendazole was used as a positive control. Third group and fourth group, in vitro exposure of the worms to the crude ethanolic extract V. merrillii nuts at a concentration of 25 mg/mL and 75 mg/mL, respectively. The histopathological changes of egg cells in the uterine region of stained worms was evaluated by light microscopic examination at 40x magnification. The results showed that shrinkage and disintegration of the entire egg cells in the breakage of uterus. The results indicate that the possible use of the plant as a potential anthelmintic against A. galli, the intestinal nematode parasite of domestic fowl.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Effect of Ethanolic Extract ofEmbelia Ribeson Dyslipidemia in Diabetic Rats

2002 ◽  
Vol 3 (3) ◽  
pp. 159-162 ◽  
Author(s):  
Uma Bhandari ◽  
Raman Kanojia ◽  
K. K. Pillai
Keyword(s):  
Folk Medicine ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Herbal Remedies ◽  
Lipid Lowering ◽  
Embelia Ribes ◽  
Liver And Pancreas ◽  
Tbars Values

Diabetes mellitus has been treated orally with herbal remedies based on folk medicine since ancient times.Embelia ribes burm(Myrsinaceae), known commonly as vidanga, was used in Ayurveda for its anthelmintic activity. Ayurveda describes vidanga as pungent, causes increase in digestive fire, and cures flatulence and colic. A single study reported the antihyperglycemic activity of decoction ofE. ribesin glucose-induced hyperglycemic albino rabbits. In the present study, the lipid-lowering and antioxidant potential of ethanolic extract ofE. ribes burmwas investigated in streptozotocin (40 mg/kg, IV, single injection)-induced diabetes in rats. Twenty days of orally feeding the extract (200 mg/kg) to diabetic rats resulted in significant (P< 0.01) decrease in blood glucose, serum total cholesterol, and triglycerides, and increase in HDLcholesterol levels when compared to pathogenic diabetic rats. Further, the extract also lowered the liver and pancreas thiobarbituric acid–reactive substances (TBARSs) values (P< 0.01) when compared to TBARS values of liver and pancreas of pathogenic diabetic rats. The results of test drug were comparable to gliclazide (25 mg/kg, orally), a standard antihyperglycemic agent. This is the first pilot study to provide biochemical evidence of potential ofE. ribesin diabetic dyslipidemia.

scholarly journals UJI DAYA ANTIINFLAMASI EKSTRAK ETANOLIK KULIT TERONG BELANDA (Solanum betaceum, Cav.) PADA MENCIT JANTAN YANG DIINDUKSI KARAGENIN

2020 ◽  
Vol 16 (02) ◽  
pp. 86
Author(s):  
Margareta Retno Priamsari ◽  
Rina Ayu Krismonikawati
Keyword(s):  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Test Material ◽  
Edema Formation ◽  
Flavonoid Compounds ◽  
The One ◽  
Post Hoc ◽  
Solanum Betaceum

ABSTRACT Inflammation is a normal response to injury due to release of chemical mediators such as prostaglandins. Solanum betaceum Cav. skin contains flavonoid compounds. This study aims to determine the antiinflammatory power and the effect of variations in the dose of ethanolic extract of the skin of Solanum betaceum Cav on Swiss male strain mice caragenin-induced. The ethanolic extract of Solanum betaceum Cav skin was obtained using remaseration method with 96% ethanol solvent. Antiinflammatory power test using edema formation method with induction of caragenin 1% intraplantar 30 minutes before administration of the test material. Tests were carried out on 25 mice records which were divided into 5 groups namely negative control (0.5% CMC Na suspension), positive control (diclofenac sodium suspension in 0.5% CMC Na dose 7 mg/kgBW) and given suspension respectively. Dutch eggplant skin ethanolic extract in CMC Na 0.5% dose 70 mg/kgBW; 140 mg/kgBW; 280 mg/kgBW orally. The parameters observed were edema thickness measured using calipers at 30 minute intervals for 360 minutes. The results of edema thickness difference are used to calculate the Area Under Curve (AUC) and the percentage of antiinflammatory power (% AIP). Dat a were analyzed statistically with the One Way Anova test and continued by the Post Hoc Test with a 95% confidence level. The results showed that the ethanolic extract of Solanum betaceum Cav skin contains flavonoid compounds and has antiinflammatory power. The greater the dose of ethanolic extract of Solanum betaceum Cav skin, the greater the anti-inflammatory power. Keywords: Solanum betaceum Cav., antinflammation, caragenin

scholarly journals Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

2020 ◽  
Vol 3 (2) ◽  
pp. 36-40
Author(s):  
Viki Ayu Intan Permatasari ◽  
Mutia Hariani Nurjanah ◽  
Wimbuh Tri Widodo
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
Traditional Medicines ◽  
Alternative Ingredients ◽  
High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

Biological evaluation and isolation, characterization, of novel polyphenol derivatives from Schleichera oleosa (lour) oken, as anti-diabetic agents

2021 ◽  
Vol 17 ◽  
Author(s):  
Soundararajan Muthukrishnan ◽  
Thillai Sivakkumar ◽  
Vigil Anbiah
Keyword(s):  
Fasting Blood Glucose ◽  
Ethanolic Extract ◽  
Biological Evaluation ◽  
Diabetic Rats ◽  
Spectral Studies ◽  
Diabetic Rat ◽  
Drug Candidate ◽  
Aqueous Extracts ◽  
Altered Level ◽  
Schleichera Oleosa

Background: The exhibit, the treatment for diabetes, for the most part, involves insulin and different oral hypoglycemic drugs. In chronic treatment medications are losing viability, therefore searching of more effective and safer natural anti-diabetic agents has continued to be an important area of investigation Objective: The present study aimed to investigate the effect of ethanolic and aqueous extracts of Schleichera oleosa (S.oleosa) in diabetic rats and isolate and characterize the active compound from an effective extract. Method: The ethanolic and aqueous extracts were administered orally (200 and 400 mg/kg, for 28 days) to streptozotocin-nicotinamide-induced diabetic rats. Hypoglycemic effects, OGTT, alteration in body weight, lipid profile, biochemical parameters, hepatic enzymes and histopathological examination were assessed. Then isolate the active constituents from effective extract using column chromatography and preliminary structure features were investigated by FT-IR, 1H NMR, 13C NMR and LC/MS. Results: The extracts treated rats showed a significant reduction in the fasting blood glucose level (FBGL), total cholesterol, triglycerides, LDL, VLDL and stress markers (MDA, SGOT, SGPT) and significant increase in the concentrations of HDL and bodyweight when compared to the diabetic rat. And also the extract restored the altered level of antioxidant enzymes and glucose metabolizing hepatic key enzymes to close normal level. Histopathological evaluation of the pancreas revealed the regeneration of the β-cells in diabetic rats which was earlier necrosed by streptozotocin. In this study, an active constituent SMK/SO/01 isolated from effective ethanolic extract and spectral studies confirmed that it’s belonging to the polyphenolic category. Conclusion: These findings provide scientific evidence that SMK/SO/01 has to be a potential drug candidate for diabetes mellitus and its complication. Also, these results prove that S.oleosa leaves of ethanolic extract is an effective anti-diabetic activity which provides the scientific proof for the folklore medicine

Sign in / Sign up

Export Citation Format

Share Document