scholarly journals The Potenty of Sechium edule Ethanolic Extract to Repair Beta Pancreas Cells and Nitrogen Oxide Concentration in Streptozotocin-induced Diabetic Rat

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Betty Lukiati ◽  
Siti Imroatul Maslikah ◽  
Nugrahaningsih N
Keyword(s):  
Nitrogen Oxide ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Type I ◽  
Sechium Edule ◽  
Group 2

The objectives of this research were to examine the effect of Sechium edule ethanolic extract to repair beta pancreas cells damage and tomeasure nitrogen oxide (NO) concentration in streptozotocin-induced rats. Type I diabetic rats were obtained by intraperioneally injected therats with multiple low dose streptozotocin (MLD-STZ) for 5 days. In this research, rats were assigned into 5 treatment groups: untreated rats ingroup 1 were considered as negative control, while MLD-STZ induced type 1 diabetic rats in group 2 were considered as positive control. Inanother 3 groups, type 1 diabetic rats were orally treated with three doses of Sechium edule ethanolic extract (14, 28, 42 mg/kg bw) respectivelyfor 7 consecutive days. Repairing of beta pancreas cells were analyzed descriptively whereas concentration of NO were analyzed using one wayANOVA and continued with LSD test (α= 0.01). The result showed that the administration of Sechium edule ethanolic extract significantlydecreased NO concentration (P<0.01) up to 67.9 µM and repaired beta pancreas cells damage. Dose of 42 mg/kg bw of Sechium edule ethanolicextract is the effective dose for diabetic rat therapy. Key words: Sechium edule extract, diabetic mellitus, rat, beta pancreas cells

scholarly journals The Role of Sechium edule Fruits Ethanolic Extract in Insulin Production and Malondialdehyde Level in Stz-Induced Diabetic Rat

2019 ◽  
Vol 4 (1) ◽  
pp. 11
Author(s):  
Betty Lukiati ◽  
Nugrahaningsih Nugrahaningsih ◽  
Siti Nur Arifah
Keyword(s):  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Sechium Edule ◽  
Liver And Pancreas ◽  
Group A ◽  
Kidney Liver

The objectives of this research were to examine the effect of Sechium edule ethanolic extract in insulin and Malondialdehyde (MDA) levels in streptozotocin (STZ) induced diabetic rats. Type 1 diabetic rat were obtained by intraperitonially injected with multiple low dose STZ (MLD-STZ) 20 mg/kgBW for 5 days. The animals were divided into 5 groups: untreated rats in group (K) were considered as negative control, MLD-STZ induced type 1 diabetic rats in group (S) were considered as positive control. In another 3 group (A, B, C) type 1 diabetic rats were orally treated with three doses of S. edule ethanolic extract (14, 28, 42 mg/kg BW) for 7 days. The animals were sacrificed in two days after last treatment, serum were collected for measuring of the insulin concentration by ELISA method. Level of MDA on kidney, liver and pancreas were measured by TBA method. The treatment with S. edule ethanolic extracts unable to increase of insulin yet but the treatment with 42 mg/kg BW of S. edule ethanolic extract showed significantly decreased MDA levels on liver but not significant on kidney and pancreas.

scholarly journals Hypoglycaemic Property of Nutraceuticals

1970 ◽  
Vol 6 (2) ◽  
pp. 105-107
Author(s):  
Mohammad Emdadul Haque ◽  
Quazi Safayetullah ◽  
Monira Ahsan ◽  
Sheikh Nazrul Islam
Keyword(s):  
Glucose Level ◽  
Day Treatment ◽  
Positive Control ◽  
Serum Glucose ◽  
Negative Control ◽  
Diabetic Rats ◽  
Serum Glucose Level ◽  
Diabetic Rat ◽  
Black Cumin ◽  
Control Serum

Hypoglycemic property of four nutraceuticals named - black cumin, garlic, methi and karela has been investigated on an alloxan induced diabetic rat model. Glibenclamide was used as positive control and normal saline was used as negative control. Serum glucose levels of normal and diabetic induced rats were estimated by Glucose Oxidase method using a kit, and it were 4.33 ± 0.43 mmol/L and 8.27 ± 0.45mmol/L respectively. The alloxan induced diabetic rats were grouped into six, which were then treated with the four nutraceuticals and two controls. Treatment was given orally everyday for 28 days. Serum glucose level was analysed every 7 day intervals. It was observed that lowering of glucose level was significant (P = 0.01) for every 7 day treatment period. All of the nutraceuticals reduced the serum glucose level nearly to the normal value within 28 days. The rate of lowering of the glucose level was found to vary with the nutraceuticals. Garlic was found to be most active like the glibenclamide. The next active one was black cumin followed by methi and karela. The study conclude that nutraceuticals possess significant hypoglycemic property. Key words: Hypoglycaemia; Nutraceuticals; Black cumin; Garlic; Methi; Karela. Dhaka Univ. J. Pharm. Sci. 6(2): 105-107, 2007 (December)

scholarly journals Effects of Extracts of Plathymenia Reticulata Benth and Azadirachta Indica (Neem) in Glycated Hemoglobin in Diabetic Rats

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A438-A439
Author(s):  
Ezio De Martino Neto ◽  
Joyce Satil Chaves da Silva ◽  
Eliane Cristina Lourenço ◽  
Arthur Cesário de Castro Neto ◽  
Isabella Cecilio Resende Ferreira ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Azadirachta Indica ◽  
Insulin Treatment ◽  
Glycated Hemoglobin ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Male Adult ◽  
Significant Difference

Abstract Introduction: The Plathymenia reticulata benth is a herbal medicine that has properties of pancreatic islet hyperplasia and glycemic control in diabetic rats. Neem (Azadirachta indica A. Juss, Meliaceae) is a tree native to India that has several medicinal effects. Goal: To verify the effect of glycated hemoglobin levels in rats with type 1 and non-diabetic diabetes mellitus, in treatment with Plathymenia Reticulata Benth, Neem and the association between them. compared to insulin. Methodology: Diabetes was induced by intraperitoneal streptozotocin (65mg/kg) administration after a 24-hour fast. The diagnosis was made using a blood glucose value above 200mg/dl. The study was conducted in 60 male adult Wistar rats, weighing between 180 and 220 grams, divided into 9 groups, between diabetics (DM) and non-diabetic controls (NdM), and treated with Neem (300 mg/kg), cold aqueous extract of Plathymenia (100 mg/kg), water (negative control) and insulin (3 IU/day) - positive control; and association between plants. The treatment was performed by orogastric gavage for a period of 28 consecutive days, and weekly weight and daily feed intake were performed. Data were analyzed using ANOVA and Tukey-Kramer’s pos-hoc test, with a significance level of 5% using the SPSS25.0 software. The results are expressed on average ± EPM. Results: There was a significant difference in glycated hemoglobin levels in rats submitted to insulin treatment (6.18 ± 0.36) compared to those submitted to treatment with Neem (10.12 ± 1.29, p=0.047), Plathymenia+Neem (12.09 ± 0.38, p=0.006) and water (10.86 ± 1.26, p=0.015). However, no significant difference was observed between the reduction in glycated hemoglobin levels in the groups submitted to insulin treatment compared to the group treated with Plathymenia (7.30 ± 0.68, p=0.911). Conclusion: The results allow us to evaluate a non-inferiority condition in relation to the use of the Plathymenia when compared to treatment with insulin therapy, positive control in the treatment of type 1 diabetes mellitus. The Plathymeniamay present as a herbal option in the treatment of the disease and prevention of complications. Further studies are necessary to evaluate the effect of the extract on other aspects related to the pathology.

scholarly journals Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination

2017 ◽  
Vol 69 (3) ◽  
pp. 535-543 ◽  
Author(s):  
Hamidreza Sherkatolabbasieh ◽  
Lida Hagh-Nazari ◽  
Shiva Shafiezadeh ◽  
Nader Goodarzi ◽  
Mohammad Zangeneh ◽  
...  
Keyword(s):  
Olive Oil ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Interstitial Tissue ◽  
Ccl4 Administration ◽  
Group 2 ◽  
Negative Controls ◽  
Convoluted Tubules ◽  
Different Doses

The present study was carried out to investigate the nephroprotective effect of the ethanolic extract of Allium saralicum R.M. Fritsch (ASRMF) in mice. Thirty-five male mice were divided into five groups (n=7). Group 1 (positive control) received 1 mL/kg olive oil intraperitoneally (i.p.) and 0.5 mL distilled water orally; Group 2 (negative control) received CCl4 (50% in olive oil, 1 mg/kg; i.p.); Groups 3, 4 and 5 received CCl4 and 200, 800 and 1600 ?g/kg of ASRMF extract, respectively. The renal volume and cortex in Groups 1 and 2 were increased by 55% and 62% (p?0.001) following CCl4 administration, respectively, and were improved after ASRMF administration. The volume of proximal convoluted tubules (PCTs), glomeruli, vessels and interstitial tissue increased 80%, 150%, 83% and 64% (p?0.05), respectively, in CCl4-treated mice, and decreased significantly with 800 and 1600 ?g/kg of ASRMF. The length of PCTs and vessels increased 51% and 45% and decreased (p?0.05) with 200, 800 and 1600 ?g/kg of ASRMF, respectively. CCl4-treated mice lost 22.5% of glomeruli; the loss was inhibited significantly (p?0.05) by ASRMF. Urea and creatinine concentrations were increased (p?0.05) in CCl4-induced nephrotoxicity as compared to the controls, whereas different doses of ASRMF restored the levels of these biomarkers compared to the negative controls. In conclusion, ASRMF has a potent nephroprotective property and can improve renal structural and serum biomarkers in CCl4-induced nephrotoxicity in mice.

scholarly journals To Evaluate the Diuretic Activity in Ethanolic Extract of Leaves of Delonix Regia in Wistar Albino Rats

2018 ◽  
Vol 11 (2) ◽  
pp. 959-963
Author(s):  
Jayanthi M K ◽  
Siddamma Amoghimath
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Diuretic Activity ◽  
Delonix Regia ◽  
Group 4 ◽  
Group 2 ◽  
Wistar Albino Rats

To study the diuretic activity in ethanolic extract of leaves of delonix regia in wistar albino rats. After obtaining the permission from the Institutional Ethical Committee (IAEC) Wistar albino rats were randomly selected and divided into 4 groups. The animals were fasted and deprived of food and water 20hrs prior to the experiment. On the day of experimentation, Group 1 negative control received Normal saline (25ml/kg), Group 2 positive control received Furosemide 20mg/kg body weight, and Group 3 received ethanonic extract of delonix regia 100mg/kg and Group 4 received ethanonic extract of delonix regia 200 mg/kg. Delonix regia increase the excretion of sodium and water at the dose of 100mg/kg and 200mg/kg of body weight. The diuretic index is 5.93 and 6.45 at 100mg/kg and 200mg/kg of delonix regia respectively. The diuretic activity if delonix regia 100mg/kg is 0.61 and 0.67 at dose 200mg/kg, which is more than that of negative control but less when compared to positive control. Ethanonic extract of Delonix regia showed significant diuretic activity at both 100mg/kg and 200mg/kg doses.

scholarly journals Estrogenic Activity of Mahoni Seed Ethanolic Extract [Swietenia mahogany (L.) Jacq] on Uterus Weight, Bone Density and Mamae Gland Proliferation on Ovariectomized Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 75
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Rosa Gloria Sitanggang ◽  
Robbani Syahfitri Angkat
Keyword(s):  
Bone Density ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Group 2 ◽  
Post Hoc

Menopause is a hypoestrogenic condition due to decreased function of the ovary. During menopause there is no reserved ovum in the ovary, as a result the synthesis of estrogen by the follicles does not take place. Deficiency of estrogen can lead to discomfort and decrease in the women quality of life. Therefore, supplements from natural resources to reduce menopausal symptoms will be needed. The objectives of the study were to determine the effect of mahogany seeds ethanolic extract (MSEE) on the development of uterus, bone density, and mammae gland proliferation on ovariectomized rats. Extract was made by maceration using 96% ethanol as the solvent, then the study of estrogenic effect was carried out on 30 female rats which were divided into 6 groups. Group 1 (normal control), group 2 (positive control) given estradiol dose of 0.18 mg/kg body weight (BW), group 3 (negative control) given Na-CMC 1% and group 4, 5, 6 given MSEE orally for 14 consecutive days with doses of 50, 100, 200 mg/kg BW. Data were analysed using ANOVA then continued with Tukey HSD Post Hoc test to see the differences between the treatments. The results of the study showed that MSEE was able to increase the weight of the uterus, the length of estrus phase in the estrus cycle, bone density and the mammae gland proliferation of rats. The results concluded that MSEE has phytoestrogenic effect on ovariectomized rats.Keywords: phytoestrogen, ovariectomy, uterus weight, bone density, mammae proliferation

scholarly journals The Assay of Blood Plasma’s Malondialdehyde (MDA) Activity in Alloxan-Induced Diabetic Rat Given Yellow Velvet Leaf Extract (Limnocharis flava)

2020 ◽  
Vol 18 (2) ◽  
pp. 157-162
Author(s):  
Yithro Serang ◽  
Ainun Nur Hammi
Keyword(s):  
Ethanolic Extract ◽  
Negative Control ◽  
Diabetic Rats ◽  
The Body ◽  
Diabetic Rat ◽  
Thiobarbituric Acid Reactive Substance ◽  
Alternative Source ◽  
White Rats ◽  
Significant Difference ◽  
One Way Anova

It has been well-known that Genjer or yellow velvet leaf (Limnocharis flava) has a potential as an alternative source to natural antioxidants. Antioxidants, such as flavonoids, alkaloids, phenols, tannins, and others are substances which can block the presence of free radicals coming into the body. It has also been examined to be beneficial in treating metabolic syndrome diseases such as diabetes, as it improves insulin’s performance. The objective of this study is to examine the antioxidant effect of yellow velvet leaf’s ethanolic extract (Limnocharis Flava) on blood plasma’s MDA activity in alloxan-induced diabetic rats (Rattus novergicus) in three different doses. The testing animals were randomly divided into five groups, each group consisted of 6 white rats. Group 1 was the negative control and given CMC Na 0.5%, while group 2 was a positive control and given glibenclamide 0.45 mg, group 3 was yellow velvet leaf’s ethanolic extract of 32.5 mg/kg, group 4 was yellow velvet leaf’s ethanolic extract of 65 mg/kg, group 5 was yellow velvet leaf’s ethanolic extract of 130 mg/kg. Previously, all groups were induced with alloxan through intra peritoneal injection. Ethanol extract was provided once on the day of 7th, 14th, and 21st. The observed parameters were blood plasma’s MDA activity in the alloxan-induced diabetic rats (Rattus novergicus). Blood plasma’s MDA activities were assessed by using Thiobarbituric Acid Reactive Substance (TBARs) method. Then, data were collected and analyzed by using One Way ANOVA followed by a Post hoc test. The results showed that the mean values of MDA levels in the testing groups of 1,2,3,4 and 5 were 9.30 ± 0.462, 2.17 ± 0.121, 6.11 ± 0.381, 4.07 ± 0.327, and 2.75 ± 0.121, respectively. One Way ANOVA test showed a significant difference in the blood plasma’s MDA levels among the groups (p = 0,000). It is concluded that the blood plasma’s MDA levels in alloxan-induced diabetic rats can be lowered by the yellow velvet leaf’s ethanolic extract (Limnocharis Flava). Therefore, it can be employed as a traditional treatment for diabetes.

scholarly journals Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Standardized Ameliorates Hyperglycemia, Polyphagia, and Weight Loss in Diabetic Rats

Molekul ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 72
Author(s):  
Yulia Fauziyah ◽  
Sunarti Sunarti ◽  
Ita Fauzia Hanoum ◽  
Mae Sri Hartati Wahyuningsih
Keyword(s):  
Diabetes Mellitus ◽  
Weight Loss ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Tithonia Diversifolia ◽  
Chronic Hyperglycemia ◽  
Group 2

Diabetes mellitus is a state of chronic hyperglycemia which causes various complications. Traditionally, The Tithonia diversifolia (Hemsley) A Gray leaf has long been used for the treatment of diabetes. The aim of this study is to investigate the effect of the T. diversifolia leaf on blood glucose, polyphagia, and weight loss in a diabetic rat model. Rats were made diabetic with intraperitonial injection of Nicotinamide and Streptozotocin and divided into 5 groups. Group 1 were healthy rats, group 2 were diabetic rats, while groups 3, 4, and 5 were diabetic rats treated with 25, 50, and 100 mg/kg body weight of 70% ethanol extract of Tithonia diversifolia leaf respectively for 28 days. Blood was taken after treatment for measuring glucose. The ethanol extract of T. diversifolia leaf decreased blood glucose in diabetic rats (P<0.05). The ethanol extract of T. diversifolia leaf significantly suppresses polyphagia and improves diabetic rat weight (P <0.05). In conclusion, Tithonia diversifolia ethanolic extract has anti-hyperglycemic effect and ameliorated the effect of diabetes mellitus symptoms, namely polyphagia and weight loss.

scholarly journals PROTECTIVE OF ETHANOLIC EXTRACT OF SAUSSUREA LAPPA AGAINST PARACETAMOL-INDUCED HEPATIC AND RENAL DAMAGE IN MALE RABBITS

2019 ◽  
pp. 68-73 ◽  
Author(s):  
MARIAM A KADHEM
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Saussurea Lappa ◽  
Liver And Kidney ◽  
Group 2

Objective: The objective of our study is to investigate the effect of the ethanolic extract of Saussurea lappa against paracetamol-induced hepatorenal toxicity in male rabbits. Methods: Eighteen male rabbits were used for this study and were divided into three groups of six rabbits each. Group 1: Rabbits were the normal (negative control), Group 2: (Positive control) Rabbits were administered paracetamol at dose 300 mg/kg body weight (B.W) for 14 day, and Group 3: Rabbits received paracetamol at dose 300 mg/kg B.W then treated with ethanolic extract of S. lappa at dose 300 mg/kg B.W for 14 day. Results: The obtained results showed a significant decrease (p≤0.05) in B.W, red blood cells count, white blood cells count, neutrophil, total protein, and albumin with significant (p≤0.05) increase in lymphocyte, alanine aminotransferase, aspartate aminotransferase, creatinine, urea, and malondialdehyde in rabbits of positive control group, histological studies showed many pathological changes in liver and kidney when compared with negative control group. The oral administration of the ethanolic extract of S. lappa significantly protected the hepatic and kidney cells from damage, the hematological and biochemical parameters were also almost normal in extract treated rabbits compared to the control group. Conclusion: Our study indicates that the roots of S. lappa act as antioxidant substance and have hepato and renoprotective effect against toxicity induced by paracetamol.

Antidiabetic effect of Psychotria malayana Jack in induced type 1 diabetic rat

MEDISAINS ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 19
Author(s):  
Fairuz Fairuz ◽  
Hasna Dewi ◽  
Humaryanto Humaryanto
Keyword(s):  
Blood Glucose ◽  
Side Effects ◽  
Type I Diabetes ◽  
Langerhans Cell ◽  
Diabetic Rat ◽  
Type I ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Therapies for hyperglycemic treatment, including insulin and oral diabetes medications, have been confirmed to cause several side effects. Thus, finding new drugs with fewer side effects is of high importance. Salung leaf herb (Psychotria malayana Jack) reported used in traditional societies as a treatment for diabetes. However, the scientific proof of this plant for diabetes treatment is still lacking.Objective: To evaluate the antidiabetic effect of the P. malayana jack in induced type 1 diabetic rats by assessing blood glucose level and pancreatic cells in white rats.Methods: Alloxan used to induce type I diabetes. Rats randomly divided into six groups. A Group P1 received 250 mg/kg BW; group P2 received 500 mg/kg BW, group P3 received 1000 mg/kg BW. While group 4 basal received no treatment, group 5 received distilled water as a negative control, and group 6 received glibenclamide as a positive control. Medications are given for six days. Glucose levels were measured, and observation of pancreatic Langerhans cell damages.Results:  A decrease in blood glucose levels observed in all treatment groups. The most significant reduction (49.76%; 1000 mg/kg BW) occurred in the P3 group. Morphological features of pancreatic Langerhans cell damage were slightly high in the P1 group.Conclusion: P. malayana Jack can consider having an antidiabetic effect in a type 1 diabetic rat by reducing blood glucose levels.

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