scholarly journals EFFECTS OF A TYPICAL ANTIPSYCHOTIC AND THROMBOXANE A2 SYNTHASE INHIBITOR DRUG COMBINATION ON ALBUMIN AND TOTAL PROTEIN LEVEL WITH DIFFERENT DOSES IN RATS

2019 ◽  
pp. 316-319
Author(s):  
MUHAMMAD IRFAN BASHIR
Keyword(s):  
Total Protein ◽  
Protein Level ◽  
Maximum Dose ◽  
Thromboxane A2 ◽  
Treated Group ◽  
Albino Rats ◽  
Typical Antipsychotic ◽  
Blood Samples ◽  
Total Protein Level ◽  
Synthase Inhibitor

Objective: The objective of this study was to investigate the combined as well as individual effects of a typical antipsychotic and thromboxane A2 synthase inhibitor on albumin and total protein level with minimum and maximum dose comparison in rats. Methods: This study consisted of 100 albino rats of 300 to 340 g from both gender, there were 10 groups, each contained 10 rats (n=10). Rats were treated with defined dose of zuclopenthixol (Zuclo) and ozagrel (Ozg) for 21 days (3 weeks). Blood samples were collected at 0, 7th, 14th, and 21st days of study. Albumin and total protein level were examined from blood samples using standard laboratory procedure. Results are extracted by applying statistical analysis on data and comparing percentage variation from 0-day value. Results: A typical antipsychotic-treated group showed gradually significant increase in albumin and total protein level, TXA2 synthase inhibitor-treated group also showed significant gradually increase in albumin and total protein level in combination groups, they showed highly significant increase p<0.001 in both parameter with maximum dose. Conclusion: Combination treatment with zuclopenthixol (Zuclo) and ozagrel (Ozg) can cause large increase on albumin and total protein level with maximum dose as compare to individual drug treatment.

scholarly journals Protective Effect of Terminalia Chebula Extract on Serum Total Protein against

2017 ◽  
Vol 46 (1) ◽  
pp. 11-14
Author(s):  
Tania Yeasmin ◽  
Kazi Shamima Akhter ◽  
Masud Imtiaz
Keyword(s):  
Protective Effect ◽  
Total Protein ◽  
Protein Level ◽  
Control Group ◽  
Albino Rats ◽  
Terminalia Chebula ◽  
Serum Total Protein ◽  
Medical College ◽  
Total Protein Level ◽  
Serum Total Protein Level

Terminalia Chebula extract is used for regeneration of hepatic cells and protection of liver against damage due to its active component. This study aims to observe the protective effect of Terminalia Chebula against Paracetamol induced change of serum total protein level in Wister Albino rats. The study was carried out in the Department of Physiology, Dhaka Medical College (DMC) during January 2013 to December 2013. A total number of 44 rats, age ranging from 90 to 120 days, weight between 150 to 200 gm (initial body weight) were selected for the study. After acclimatization for 14 days, they were divided into control groups and experimental groups. Before sacrifice, final body weights of all the rats were measured. then all the rats were sacrificed on 22nd day and then blood samples were collected. For assessment of liver function, serum total protein level was done by using standard laboratory kits. The mean serum total protein level was significantly (p<0.001) lower in paracetamol treated control group in comparison to those of baseline control group. Serum total protein level of all experimental groups were significantly (P<0.001) higher than Paracetamol treated control group. From the results of this study, it may be concluded that Terminalia Chebula may have some protective effect against Paracetamol induced liver damage in rats.Bangladesh Med J. 2017 Jan; 46 (1): 11-14

scholarly journals Effects Of High Dosage of Codeine - Containing Cough Syrup Administration on Some Biochemical Parameters of Liver in Albino Rats

2021 ◽  
Vol 9 (2) ◽  
pp. 30-33
Author(s):  
Aishatu Muhammad Bello ◽  
Ramlatu Musa Adam ◽  
Fatima Umar Maigari ◽  
Idi Jalil James ◽  
Abubakar Aisami
Keyword(s):  
Alkaline Phosphatase ◽  
Pain Management ◽  
Total Protein ◽  
Protein Level ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Albino Rats ◽  
Total Protein Level ◽  
Cough Syrup

The study aimed to determine the effect of a high dosage of codeine-containing cough syrup administration on some biochemical parameters of the liver in albino rats. Codeine at 80 mg/kg/day, 160 mg/kg/day, 240 mg/kg/day, 320 mg/kg/day cough syrup were administered orally to albino rats for 21 days, biochemical parameters were analyzed for the activities of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Bilirubin, Total protein and Albumin. Results obtained revealed that a high dosage of codeine administration significantly increased plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and albumin while it reduced total protein level when compared with the control rats. The study confirmed the risk of increased hepatotoxicity due to a high dosage of codeine administration. Although codeine is reported to be effective in pain management, its toxicity should be kept in mind.

scholarly journals An atherogenic stimulus homocysteine inhibits cofactor activity of thrombomodulin and enhances thrombomodulin expression in human umbilical vein endothelial cells

Blood ◽  
1992 ◽  
Vol 79 (11) ◽  
pp. 2930-2936 ◽  
Author(s):  
T Hayashi ◽  
G Honda ◽  
K Suzuki
Keyword(s):  
Endothelial Cells ◽  
Total Protein ◽  
Protein Level ◽  
Umbilical Vein ◽  
Mrna Level ◽  
Fold Increase ◽  
Immunoblot Analysis ◽  
Whole Cells ◽  
Total Protein Level ◽  
Cofactor Activity

Abstract Thrombomodulin plays a role as a cofactor for thrombin-catalyzed activation of protein C on endothelial cells. We examined the effect of homocysteine, a stimulant of atherosclerosis and thrombotic disease, on the cofactor activity and protein level of thrombomodulin and also on the expression of thrombomodulin in endothelial cells. Homocysteine inhibited the cofactor activity of thrombomodulin both on the surface of endothelial cells and in the whole cells dose- and time-dependently, and maximal inhibition of the cofactor activity occurred after a 3- to 6-hour incubation with 10 mmol/L homocysteine (10% of initial activity). Homocysteine also decreased the amount of intact (unreduced) thrombomodulin in endothelial cells. However, at the same condition the total protein level (reduced and unreduced form) of thrombomodulin, determined by dot immunoblot analysis using the monoclonal antibody that recognized both reduced and unreduced thrombomodulin, decreased slightly, and the mRNA level of thrombomodulin showed a twofold to three-fold increase. After 24 hours of incubation, the cofactor activity and total protein level of thrombomodulin were 60% and 165% of the initial values, respectively. When purified thrombomodulin fixed to a microwell plate was treated with homocysteine, both cofactor activity and thrombin-binding ability to the thrombomodulin were decreased in proportion to the concentration of homocysteine. These findings suggest that homocysteine directly inhibited the cofactor activity of thrombomodulin on endothelial cells by reducing the disulfide-bond rich epidermal growth factor-like structures of thrombomodulin. This would a result in the decrease of the antithrombotic property of endothelium and may also trigger off the synthesis of mRNA and protein of thrombomodulin to maintain the antithrombotic properties of the cells.

scholarly journals An atherogenic stimulus homocysteine inhibits cofactor activity of thrombomodulin and enhances thrombomodulin expression in human umbilical vein endothelial cells

Blood ◽  
1992 ◽  
Vol 79 (11) ◽  
pp. 2930-2936 ◽  
Author(s):  
T Hayashi ◽  
G Honda ◽  
K Suzuki
Keyword(s):  
Endothelial Cells ◽  
Total Protein ◽  
Protein Level ◽  
Umbilical Vein ◽  
Mrna Level ◽  
Fold Increase ◽  
Immunoblot Analysis ◽  
Whole Cells ◽  
Total Protein Level ◽  
Cofactor Activity

Thrombomodulin plays a role as a cofactor for thrombin-catalyzed activation of protein C on endothelial cells. We examined the effect of homocysteine, a stimulant of atherosclerosis and thrombotic disease, on the cofactor activity and protein level of thrombomodulin and also on the expression of thrombomodulin in endothelial cells. Homocysteine inhibited the cofactor activity of thrombomodulin both on the surface of endothelial cells and in the whole cells dose- and time-dependently, and maximal inhibition of the cofactor activity occurred after a 3- to 6-hour incubation with 10 mmol/L homocysteine (10% of initial activity). Homocysteine also decreased the amount of intact (unreduced) thrombomodulin in endothelial cells. However, at the same condition the total protein level (reduced and unreduced form) of thrombomodulin, determined by dot immunoblot analysis using the monoclonal antibody that recognized both reduced and unreduced thrombomodulin, decreased slightly, and the mRNA level of thrombomodulin showed a twofold to three-fold increase. After 24 hours of incubation, the cofactor activity and total protein level of thrombomodulin were 60% and 165% of the initial values, respectively. When purified thrombomodulin fixed to a microwell plate was treated with homocysteine, both cofactor activity and thrombin-binding ability to the thrombomodulin were decreased in proportion to the concentration of homocysteine. These findings suggest that homocysteine directly inhibited the cofactor activity of thrombomodulin on endothelial cells by reducing the disulfide-bond rich epidermal growth factor-like structures of thrombomodulin. This would a result in the decrease of the antithrombotic property of endothelium and may also trigger off the synthesis of mRNA and protein of thrombomodulin to maintain the antithrombotic properties of the cells.

scholarly journals ELEKTROFORESIS PROTEIN SERUM PASIEN DENGAN KADAR PROTEIN NORMAL

2018 ◽  
Vol 15 (3) ◽  
pp. 87
Author(s):  
Tiene Rostini ◽  
Coriejati Rita
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Protein Level ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Total Protein Level ◽  
Normal Limits ◽  
Nephritic Syndrome ◽  
Serum Protein Level ◽  
Electrophoresis Pattern

Serum protein electrophoresis pattern can assist in diagnosis of liver disease, hematological disorders, renal disorders andgastrointestinal disease. Measurement of total protein level in the serum cannot detect any disorders in patient with normal limit ofserum total protein level. The aim of this study; was to evaluate the serum protein electrophoresis pattern in patient with normal limitsof serum protein level. This research was carried out by descriptive retrospective study using the electrophoresis data from patients’medical record at the Clinical Pathology Department, Dr. Hasan Sadikin General Hospital Bandung. The data of serum electrophoresis (bySebia gel electrophoresis) were grouped based on disease or disorders, and confirmed with the diagnosis derived from patient’s medicalrecord. Inclusion criteria of samples if ; the electrophoresis data were available, serum total protein level within normal limits (6.4–8.3mg/dL), and the data of electrophoresis taken from medical record were taken from August 2006 until August 2008. The result foundso far was, there were 240 data of electrophoresis from patients with serum protein level within normal limits (6.4–8.3 mg/dL). theinterpretation of electrophoresis consist of: 1) inflammation (149 patients; 62.2% ; sensitivity 83.7%, specificity 86,5%) 2) Cirrhosis(46 patients ; 19.2% ; sensitivity 87.5% ; specificity 88.4%) 3) Nephritic syndrome (15 patients ; 6.2%; sensitivity 53%; specificity96.9% 4) Monoclonal gammophaty (15 patients(6.2% ; sensitivity 80% ; specificity 98.7%) 5) Normal pattern in 15 patient (6.2%).This study found abnormal serum protein electrophoresis pattern in the condition of inflammation, Cirrhosis, Nephritic Syndrome, andMonoclonal gammophaty. It can be concluded that many disorders could be detected in patient with serum protein level within normallimits such as: inflammation, cirrhosis, nephritis syndrome and monoclonal gammophaty by abnormal electrophoresis pattern

Total Protein Level in Cerebrospinal Fluid is Stable in Elderly Adults

2013 ◽  
Vol 61 (10) ◽  
pp. 1819-1821 ◽  
Author(s):  
Diane Dufour-Rainfray ◽  
Emilie Beaufils ◽  
Patrick Vourc'h ◽  
Emilie Vierron ◽  
Laurent Mereghetti ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
Protein Level ◽  
Elderly Adults ◽  
Total Protein Level

scholarly journals Effect of Flax Seed Oil on Acute Carbon Tetrachloride-induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Gorkem Ekebas ◽  
Ayhan Atasever ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Total Cholesterol ◽  
Total Protein ◽  
Caspase 3 ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Treated Group ◽  
Flaxseed Oil ◽  
Albino Rats ◽  
Intraperitoneal Dose

Aims: The present study was designed to evaluate the hepatoprotective activity of flaxseed oil (FSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats by measurement of caspase 3, 8 and 9 activities in cellular apoptosis, ALT activities, triglyceride, total protein, total cholesterol and liver MDA levels. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between June 2017 and July 2018. Methodology: In this study 32 male Wistar albino rats were divided into four groups of 8 animals in each. The first group was identified as the control and received an intraperitoneal 0.9% NaCl and the second group was given per os at dose of 4 ml/kg FSO for 4 weeks. The third group received an intraperitoneal dose of 1.0 ml/kg CCl4 twice in the first week. The fourth group received an intraperitoneal dose of 1.0 ml/kg CCl4 twice in the first week and simultaneously 4 ml/kg FSO by gavage for 4 weeks. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, lymphocytes rich mononuclear cell infiltration in portal area and parenchyma. The flaxseed oil application did not ameliorate the histological changes induced by CCl4, however reduced the activity of caspase 3, 8 and 9 by a limited number. CCl4 administration produced significantly elevated levels of serum ALT activity, total cholesterol, triglyceride and liver MDA levels, and these increases were not normalized with FSO treatment. In addition, decreased serum total protein levels in CCl4 treated group were ameliorated by FSO application. Conclusion: The results indicate that the antioxidant properties of FSO do not have an ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. In addition, it was concluded that duration‐dependent further research results are needed to determine the effects of flaxseed oil in high doses that can give the best results without side effects.

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