scholarly journals EVALUATION OF THE ANTICANCER PROPERTIES OF TURMESAC® IN HUMAN CERVICAL ADENOCARCINOMA

2020 ◽  
pp. 109-113
Author(s):  
FIROZ HM ◽  
NANJUNDAIAH S ◽  
SADASHIVA CT
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Anticancer Agent ◽  
Cervical Adenocarcinoma ◽  
Apoptosis Induction ◽  
Anticancer Properties ◽  
Cycle Arrest ◽  
Diphenyltetrazolium Bromide ◽  
Ic50 Value

Objective: In the current study, an extract of turmeric rhizome (Turmesac®) was evaluated for possible anticancer activity in human cervical adenocarcinoma (HeLa) cells. Methods: Turmesac®’s ability to elicit cytotoxicity in cancer cells was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (MTT) assay, where the IC50 value was determined. Apoptosis induction and cell cycle arrest were analyzed by flow cytometry post-Turmesac® IC50 value treatment for 24 h. Results: The determined IC50 value of Turmesac® in HeLa cells was 115.12 μg/ml. This concentration was able to induce apoptosis 2 times greater than the apoptotic standard, camptothecin, treated cells. Cell cycle arrest was observed at the G0/G1 and S phases in Turmesac® treated HeLa cells. Conclusion: Turmesac® shows the potential of being a promising anticancer agent that may be incorporated into chemotherapies, but further study is required to elucidate the exact mechanisms involved with longer treatment duration, as would be the case in clinical trial phases.

scholarly journals A class of DNA-binding peptides from wheat bud causes growth inhibition, G2 cell cycle arrest and apoptosis induction in HeLa cells

2009 ◽  
Vol 8 (1) ◽  
pp. 55 ◽  
Author(s):  
Loretta Mancinelli ◽  
Paula M De Angelis ◽  
Lucia Annulli ◽  
Valentina Padovini ◽  
Kjell Elgjo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Binding ◽  
Growth Inhibition ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Apoptosis Induction ◽  
Cycle Arrest ◽  
Binding Peptides ◽  
G2 Cell Cycle Arrest

scholarly journals Euphornin reduces proliferation of human cervical adenocarcinoma HeLa cells through induction of apoptosis and G2/M cell cycle arrest

2018 ◽  
Vol Volume 11 ◽  
pp. 4395-4405 ◽  
Author(s):  
Xiao-Qiang Li ◽  
Yin-Liang Bai ◽  
De-Li Zhang ◽  
Hai-Sheng Jiao ◽  
Rong-Xia He
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
M Cell ◽  
Cycle Arrest ◽  
Induction Of Apoptosis

scholarly journals Antitumor activity, cell cycle arrest and apoptosis induction in human colon cancer cell line by Primula macrophylla extracts

2017 ◽  
Vol 12 (2) ◽  
pp. 4
Author(s):  
Di-Wen Shou ◽  
Yue-Lin Zheng
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Methanol Extract ◽  
Cancer Cell Line ◽  
Human Colon ◽  
Colon Cancer Cell Line ◽  
Human Colon Cancer Cell ◽  
Apoptosis Induction ◽  
Human Colon Cancer ◽  
Cycle Arrest

<p class="Abstract">The primary objective of the current work was to investigate the antitumor potential of <em>Primula macrophylla</em> extracts in human colon cancer cell line (Colo-205) along with evaluating the effects on apoptosis induction, cell cycle arrest and mitochondrial membrane potential. Cell viability was assessed by tetrazolium-based MTT assay. Flow cytometry measurement was carried out to assess the effect of the extract on cell cycle phase distribution and mitochondrial transmembrane potential. Results showed that <em>P.  macrophylla</em> methanol extract was effective and exhibited highest cell growth inhibition (IC<sub>50</sub> value, 26.17 μg/mL). Methanol extract significantly increased the side-scattering profile of Colo-205 cells in concentration-dependent pattern. Exposure of Colo-205 cells with different concentrations of the methanol extract (0-80 μg/mL) caused dose-dependent G0/G1 cell cycle arrest along with inducing apoptotic cascade by increasing the population of cells at G0/G1 phase. Furthermore, methanol extract treatment caused an increase in mitochondrial membrane depolarization in Colo-205 cells.</p><p class="Abstract"><strong>Video Clip of Methodlogy</strong> (3 min 17 sec): <a href="https://youtube.com/v/yy-rCjDN830">Click</a>  <a href="https://youtube.com/watch?v=yy-rCjDN830">If failed</a></p>

scholarly journals Sophisticated Framework between Cell Cycle Arrest and Apoptosis Induction Based on p53 Dynamics

PLoS ONE ◽  
2009 ◽  
Vol 4 (3) ◽  
pp. e4795 ◽  
Author(s):  
Hiroyuki Hamada ◽  
Yoshihiko Tashima ◽  
Yu Kisaka ◽  
Kazunari Iwamoto ◽  
Taizo Hanai ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Apoptosis Induction ◽  
Cycle Arrest

scholarly journals Syringin exhibits anticancer effects in HeLa human cervical cancer cells by inducing apoptosis, cell cycle arrest and inhibition of cell migration

2016 ◽  
Vol 11 (4) ◽  
pp. 838 ◽  
Author(s):  
Ning Xia
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Migration ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Dependent Manner ◽  
Cycle Arrest ◽  
Cervical Cancer Cells ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

<p class="Abstract">The present study was aimed at to demonstrate the antitumor effects of syringin in HeLa human cervical cancer cells. Its effects on apoptosis, cell cycle phase distribution as well as on cell migration were also examined. The effect on cell proliferation was evaluated by MTT assay, while as effects on colony formation were assessed using clonogenic assay. Syringin inhibited cancer cell growth in HeLa cells in a time-dependent as well as in a concentration-dependent manner. Syringin also led to inhibition of colony formation efficacy with complete suppression at 100 µM drug dose. Syringin could induce G2/M cell cycle arrest along with slight sub-G1 cell cycle arrest. HeLa cells began to emit red fluorescence as the dose of syringin increased from 0 µM in vehicle control to 100 µM. Syringin also inhibited cell migration in a dose-dependent manner with 100 µM dose of syringin leading to 100% inhibition of cell migration.</p><p> </p>

scholarly journals Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

2012 ◽  
Vol 13 (10) ◽  
pp. 5131-5136 ◽  
Author(s):  
Aied M. Alabsi ◽  
Rola Ali ◽  
Abdul Manaf Ali ◽  
Sami Abdo Radman Al-Dubai ◽  
Hazlan Harun ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Anticancer Activity ◽  
Cell Cycle Arrest ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Apoptosis Induction ◽  
Cycle Arrest
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