scholarly journals DETERMINATION OF ANTIMICROBIAL ACTIVITY IN LEAVES AND FLOWERS OF CHROMOLAENA SCABRA (L. F.) R.M. KING AND H. ROB.

2020 ◽  
pp. 53-56
Author(s):  
PAULA ALEJANDRA GIRALDO VILLAMIL ◽  
ANDRÉS CAMILO ANDRADE BURBANO ◽  
LUIS POMBO OSPINA ◽  
JANETH ARIAS PALACIOS ◽  
ÓSCAR EDUARDO RODRÍGUEZ AGUIRRE
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Fungal Growth ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Ic50 Values ◽  
Bacteria And Fungi

Objective: The objective of the study was to determine the antimicrobial activity of leaf and flower extract in Chromolaena scabra (L. f.) R.M. King and H. Rob., against selected strains of bacteria and fungi. Methods: The agar diffusion method with plate perforation was developed; the microorganisms used were strains of Staphylococcus aureus and Escherichia coli, Aspergillus niger, and Penicillium digitatum. Rifampicin was used as a positive control. The evaluation was performed by measuring the diameter of the growth inhibition zones around the holes. The inhibitory effect of the plant extracts was obtained by its efficiency compared to the positive control. A comparison with fluconazole and ketoconazole was performed to determine how much of the extract is required to cause inhibition of fungal growth from the standard. Results: IC50 was determined by relating the ln of mass evaluated with respect to the square of the inhibition halo; ethanolic extracts of leaves and flowers of petroleum ether with IC50 values of 85.8 mg/ml and 50.3 mg/ml showed the highest inhibitory effect against S. aureus; the extract of petroleum ether and ethanol from leaves with IC50 of 64 mg/ml and 60 mg/ml, respectively. They were effective with A. niger. Leaf petroleum ether extract showed the best relative antifungal activity against A. niger with respect to fluconazole equivalent to 459.51 when fluconazole is 1.0. Conclusion: The extracts with high potential to inhibit the growth of microorganisms were determined to be ether flowers of petroleum and ethanol leaf extracts.

scholarly journals EVALUATIONS OF SECONDARY METABOLITES ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL IN LEAF EXTRACTS OF CORBICHONIA DECUMBENS (FORSSK.) EXELL

2020 ◽  
pp. 181-183
Author(s):  
VIDYACHARANI DHA ◽  
ARUNPRASATH A
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Pathogenic Bacteria ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Scavenging Activity ◽  
Ether Extract ◽  
Leaf Extracts ◽  
Soxhlet Apparatus

Objectives: The present work is to evaluate the presence of phenol and flavonoid contents, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) activity and antimicrobial activity of Corbichonia decumbens leaf extracts. Methods: C. decumbens leaves were collected, shaded, dried, powdered, and were subjected to Soxhlet apparatus for extraction using ethanol and petroleum ether. The ethanol and petroleum ether extract of the plant was tested for phenol, flavonoid, H2O2 contents, and DPPH scavenging activity using standard procedures. It is also subjected to antimicrobial activity against pathogenic bacteria and fungus using the well diffusion method. Results: The result revealed that the leaf extracts contain phenol, flavonoid, and H2O2 contents. The values of DPPH radical scavenging activity recorded for ethanol and petroleum ether extract were 55.90±0.10 and 41.23±0.20, respectively. Antimicrobial activities of ethanolic leaf extracts showed lesser values compared to standard values. Conclusion: The result of this study showed the presence of phenol and flavonoid content, evidenced the potential antioxidant and antimicrobial activity against all tested micro-organisms.

IN VITRO ANTIMICROBIAL ACTIVITY OF CYNODON DACTYLON (L.) PERS. LEAF EXTRACTS. ANTIMICROBIAL ACTIVITY BY ZONE OF INHIBITION ESTIMATION

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (04) ◽  
pp. 37-41
Author(s):  
S. M Pawaskar ◽  
◽  
K. C. Sasangan
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Cynodon Dactylon ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
In Vitro Antimicrobial Activity

The present study was undertaken to evaluate in vitro antimicrobial activity of the successive leaf extracts of Cynodon dactylon in petroleum ether, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive & gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method & Agar disc diffusion method were used to evaluate the antibacterial efficacy. The microorganisms used in the test were – Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Bacillus subtilis, Streptococcus pyogenes, Vibrio cholerac and Enterobacter aerogenes. Two fungal strains - Candida albicans and Saccharomyces cerevisiae were also tested to evaluate the antifungal potential of the said plant extract. The reference antibiotics chloramphenicol & ampicillin (antibacterial); nystatin & clotrimazole and (antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts.The results of the study revealed that all the seven successive extracts of the leaf powder of Cynodon dactylon ( L.) Pers. exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. The nonpolar extracts i.e. petroleum ether, ethyl acetate and acetone showed the most significant antibacterial and antifungal activity against all tested organisms. The petroleum ether and ethyl acetate extracts showing maximum inhibition in the range of 8 mm - 15 mm. This was closely followed by, acetone extract, which showed the inhibition in the range of 9 mm - 13 mm.

scholarly journals Screening of multidrug-resistant bacteria of antibiotic-associated diarrhea and their inhibition by leaf extracts of Moringa oleifera Lamk

2014 ◽  
Vol 20 ◽  
pp. 41-48
Author(s):  
MM Akhtar ◽  
MS Islam ◽  
MF Begum ◽  
M Anisuzzaman ◽  
MF Alam
Keyword(s):  
Petroleum Ether ◽  
Moringa Oleifera ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Leaf Extracts ◽  
Multidrug Resistant Bacteria ◽  
Fresh Leaf

Context: Emergence of multi drug resistance bacteria (MDRB) to human pathogenic infection is increasing day by day but the number of new drugs to overwhelm the problem is not sufficient. Evidences revealed that Moringa oleifera Lamk. has various pharmaceutical activities like antibacterial, antifungal, antispasmodic, anti-inflammatory, anticancer and diuretic. Herbal treatment may be one of the possible ways to treat diseases caused by multidrug-resistant bacteria. Objectives: The present research was undertaken to screen of multidrug resistant bacteria (MDRB) from antibiotic-associated diarrheal samples and to evaluate the potentiality of M. oleifera leaf extracts on these bacteria with the view to provide scientific evidence for its application in health remedy. Materials & Methods: Antibiotic-associated diarrheal fecal specimens were collected from pediatric ward of Rajshahi Medical College and cultured onto MacConkey agar. MDRB were determined by antibiotic susceptibility test, using disc-agar diffusion method. Biochemical tests of the MDRB were done according to Bergey’s Manual of Determinative Bacteriology for identification of the species. Dried and fresh leaf of M. oleifera was used to prepare exaction with or without solvents such as hot water, cold water, chloroform, petroleum ether, acetone and ethanol, separately. Antibacterial assay was done by disc diffusion method and minimum inhibitory concentration of the extracts was also measured. Results: In the present study seven isolates were screened as MDRB and the highest prevalence (42.86%) was occurred in the age group of 25-36 months and the lowest (14.28) was in the group of <1 and 1-6 months. Ethanol extract of dried leaf of M. oleifera Lamk. showed moderate inhibitory activity against all of the isolates while petroleum ether, chloroform and acetone extracts of dried leaf have no inhibitory effect. Fresh leaf sap powder in DMSO exhibited strong inhibitory effect against all of the test bacteria where as hot aqueous extract could not show any inhibition. The minimum inhibitory concentrations (MIC) of the potent extracts ranged 937.5 to 3750 ?g/ml and 7.9 to 234.4 ?g/ml in dried and fresh leaf extracts, respectively. Conclusion: The present data indicates that M. oleifera leaf extract possess antimicrobial potential to control of MDRB causes infection thus it can be used as a novel drugs in future. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17652 J. bio-sci. 20: 41-48, 2012

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY AGAINST CLINICAL PATHOGEN OF MEDICINALLY IMPORTANT ORCHID CALANTHE MASUCA (D.DON) LINDL

2021 ◽  
pp. 5-8
Author(s):  
V. Nandagopalan ◽  
D. Kavitha
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Ether Extract ◽  
Wide Range

Calanthe masuca (D.Done) Lindl is a potential medicinal plant of terrestrial orchids. Tribal peoples were used the plants for therapeutic purpose. The present study was to evaluate the phytochemical analysis and Antimicrobial Activity of Calanthe masuca in leaf extracts. Preliminary phytochemical analysis revealed that the presence of bioactive substance in leaf extract was found that Alkaloids, Flavanoids, Phenol, Tannins, Saponins, Steroids, Terpenoids, Carboxcylic acid, Glycoside and Mucilage. Antimicrobial activity by Agar well diffusion method again six pathogenic microbes such as Staphylococcus aureus, Bacillus subtilis, E.coli and Pseudomonas aeruginosa in bacteria, Aspergillus niger and Candida albicans in fungi by using different solvent such as Aqueous, Methanol and Petroleum ether. It was observered that Methanol extract showed highest activity against the pathogen such as S.aureus (13.5 mm), B.subtilis (12.4 mm), E.coli (12.5 mm) and P.aeruginosa (13.0 mm) and then followed by the Aqueous extract and low activity showed in petroleum ether extract. The methanol extract showed signicant Antifungal activity against Aspergillus niger (14.0mm) and Candida albicans (13.0 mm) with higher concentration and Petroleum ether extract showed lower activity in fungi. The capacity of the crude concentrates of Calanthe masuca to suppress the development of microorganism which means that it has a wide range of antimicrobial potential utilized for various cures.

scholarly journals Evaluation of Antibacterial Properties of Different Leaf Extracts of Hyptis suaveolens (L) Poit

2021 ◽  
pp. 40-44
Author(s):  
B. A. Erinle ◽  
A. O. Ajayi ◽  
O. T. Osuntokun
Keyword(s):  
Antimicrobial Activity ◽  
Folk Medicine ◽  
Antibacterial Properties ◽  
Extraction Process ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Hyptis Suaveolens

Aim: Hyptis suaveolens is well known for its immense medicinal properties and are beneficial as folk medicine. The objective of the study is to investigate the efficacy of the leaves for it antimicrobial activity. Material and Methods: The leaves of Hyptis suaveolens was  subjected to extraction process using n-hexane, ethanol and distilled water as solvents and the antimicrobial activity was analyzed against different bacterial strains viz.; Escherichia coli, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Enterococcus faecalis by agar well diffusion method. Results: Aqueous extracts showed inhibitory effect against the different tested bacteria organisms with variable zone of inhibitory range 6 -21 mm. Conclusion: The aqueous extracts of Hyptis suaveolens exhibited the presence of highly effective bio-active compounds in these extracts. These can further be evaluated and characterize to improve upon what has been done to create a novel compound that can be useful for various medicinal purposes.

scholarly journals Phytochemical screening and evaluation of Berginia ligulata root extract for antimicrobial activity

2013 ◽  
Vol 14 (3) ◽  
pp. 101-104
Author(s):  
Rishi Kumar Shukla
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Diethyl Ether ◽  
Pathogenic Bacteria ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Root Extract ◽  
Phytochemical Screening ◽  
Bacterial Strains

Berginia ligulata popularly known as paashaanbhed belongs to family Saxifragaceae. The present study was designed to screen out various phytoconstituents present in the roots of Berginia ligulata and further its antimicrobial activity against human pathogenic bacteria. For this roots of the plant were shade dried, powdered and extracted using different solvents in increasing order of polarity i.e. petroleum ether, diethyl ether, acetone, ethanol and methanol respectively. Preliminary phytochemical screening of the extracts revealed the presence of steroids, terpenoids, alkaloids, tannins, flavanoids, glycosides and carbohydrates. The presence of these bioactive constituents is associated with the antimicrobial activity of the plant. Antimicrobial activity of extracts was performed by disc diffusion method at a concentration of 100% and 50% (w/v) against five bacterial strains. The maximum antimicrobial activity was found in ethanol extract followed by acetone, methanol, diethyl ether and least in petroleum ether extract respectively. Ethanol extract revealed broad spectrum of inhibition against S. sureus and P. aeruginosa.

Antimicrobial Activity of Extracts of the Lichen Parmelia sulcata and its Salazinic Acid Constituent

2007 ◽  
Vol 62 (7-8) ◽  
pp. 619-621 ◽  
Author(s):  
Mehmet Candan ◽  
Meral Yılmaz ◽  
Turgay Tay ◽  
Murat Erdem ◽  
Ayşen Özdemir Türk
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Aeromonas Hydrophila ◽  
Petroleum Ether Extract ◽  
Ether Extract ◽  
Proteus Vulgaris ◽  
Penicillium Notatum ◽  
Parmelia Sulcata ◽  
Food Borne ◽  
Bacteria And Fungi

The antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen Parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. All of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Proteus vulgaris, Yersinia enterocolitica, Staphylococcus aureus, Streptococcus faecalis, Candida albicans, Candida glabrata, Aspergillus niger, Aspergillus fumigatus, and Penicillium notatum. Salazinic acid did not show antimicrobial activity against L. monocytogenes, P. vulgaris, Y. enterocolitica, and S. faecalis but showed activity against Pseudomonas aeruginosa and Salmonella typhimurium as well. The MIC values of the extracts and the acid for the bacteria and fungi have also been determined

scholarly journals Antimicrobial and Cytotoxic Activities of Root Extracts of Piper Chaba

2008 ◽  
Vol 1 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Tarannum Naz ◽  
Ashik Mosaddik ◽  
M. Ekramul Haque
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Primary Standard ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Ether Extract ◽  
Antifungal Properties ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal

  Antibacterial and antifungal properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Piper chaba (Choi) roots were studied by disc diffusion method and these activities were compared with primary standard drugs Kanamycin and Nystatin, respectively. The extracts were found to exhibit promising antibacterial and antifungal properties against Gram-positive, Gram-negative bacteria and fungi. The extracts were also studied for their cytotoxic activities by brine shrimp lethality bioassay, where gallic acid was used as primary standard. It was observed that the petroleum ether extract was potent cytotoxic with the LC50 value of 0.95 µg/ml against Artemia salina (L). The essential oils of the petroleum ether extract of Piper chaba roots were analysed by GC/MS. It was observed that most of the compounds were sesquiterpenes, some were long chain fatty acids and some were monoterpenes and alkaloids. Seventeen compounds were identified from the GC/MS analysis. Keywords: Piper chaba, antibacterial activity, antifungal activity, cytotoxicity. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1446

scholarly journals The antimicrobial and phytonutrient profile of Ocimum gratissimum

2021 ◽  
Vol 14 (2) ◽  
pp. 045-050
Author(s):  
Ujong UP ◽  
Okon VE ◽  
Odom GE ◽  
Igwe CO
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Antifungal Drugs ◽  
New Drugs ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Klebsiella Pneumonia ◽  
Leaf Extracts ◽  
Plant Materials

Many plant-derived compounds have been used as drugs, either in their original or semi-synthetic forms. Plant derived metabolites can also serve as lead compounds, which may be used as templates for the development of new drugs. Therefore, the aim of this study was to evaluate the phytochemical and antimicrobial activities of extract of O. gratissimum leaves on selected clinical pathogenic organisms. The plant materials were obtained through successive extractions using solvents of different polarities such as petroleum ether, ethanol and distilled water. The phytoconstituents were analyzed using standard procedures while antibacterial activities of the extracts were then evaluated by the hole-agar-well diffusion method. The minimum inhibitory concentration of the extract was determined against the isolated microorganism by agar dilution method. The presence of tannins, flavonoids, saponins, glycosides, steroids, triterpenoids and alkaloids in the different leaf extracts was established at varying concentration from the results. The ethanol extract was found to be the most potent, followed by the petroleum ether extract, while aqueous extract was the least potent. Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Candida albican were highly susceptible to the ethanol extracts, since it showed the most susceptible higher zones of inhibition. The ability of the extracts to inhibit the growth of several bacterial and fungal species is an indication of the broad spectrum antimicrobial potential of leaf extract of O. gratissimum, which suggests the plant as a tool for bioprospecting for antibiotic and antifungal drugs.

scholarly journals Phytochemical Screening and Antimicrobial Activity of Ziziphus mauritiana Lam. And Ziziphus mucronate Lam. Extracts

2021 ◽  
pp. 25-37
Author(s):  
Salamatou Mohamadou ◽  
Bayoï James ◽  
Djoulde Darman Roger ◽  
Nodem Sohanang Francky Steve ◽  
Tatsadjieu Ngoune Leopold
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Proton Pump ◽  
Extraction Yield ◽  
Diffusion Method ◽  
Ziziphus Mauritiana ◽  
Leaf Extracts ◽  
Activity Assessment ◽  
Bacteria And Fungi ◽  
Multiple Pathogens

The objective of this study was to determine the phytochemical profile and antimicrobial activity of leaf and bark extracts of Ziziphus mauritiana and Ziziphus mucronata. For this purpose, an extraction by maceration using ethanol was carried out and the extracts were subjected to antibacterial activity assessment through qualitative and quantitative approaches. The qualitative tests were performed using agar well diffusion method while for quantitative tests minimal inhibitory, bactericidal and fungicidal concentration (MIC, MBC or MFC) were used determined through microdilution in microplates method. The antimicrobial mechanisms such as cell lysis and action on the proton pump of the extracts were also studied. The highest extraction yield was obtained with the Z. mauritiana leaf extract (28.8%). In addition, the highest contents of polyphenols (256.6 mg GEA/g DM) and flavonoids (165.2 mg CE/g DM) were obtained with the bark of Z. mucronata. The bark extracts of Z. mauritiana exhibited highest antibacterial activity (36.7 mm on Staphylococcus aureus) while, Z. mucronata extracts are more active on fungi, with the highest activity on Candida albicans (26.7 mm). The antimicrobial activity of the extract increases with the concentration and, regarding the MBC/MIC and MFC/MIC ratio, the extracts showed bacteriostatic activity on the different bacteria and fungi and the possible mechanism included an activity on the proton pump. The bark and leaf extracts of Z. mauritiana and Z. mucronata could be used in traditional medicine to treat infections due to these multiple pathogens.

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