scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBICIDAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF COUROUPITA GUIANENSIS

Author(s):  
SUJATHA ILANGOVAN ◽  
THAVASUMANI P

Objective: The objective of the study was the preliminary screening of the methanolic leaf extract of Couroupita guianensis for various phytochemical constituents, its antioxidant, antibacterial, and antifungal activities. Methods: Phytochemical screening of 18 qualitative, 6 quantitative constituents, antioxidant activity, alpha-amylase inhibitory activity, and alpha-glucosidase inhibitory activity of the methanolic extract of the leaf of C. guianensis was performed adopting the standard protocols. The disk diffusion methods were used for assessing the antibacterial and antifungal activities of the extract. Results: The preliminary studies revealed the presence of alkaloids, saponin, flavonoids, phenol, tannin, and terpenoids in the methanolic extract of the leaf of C. guianensis. Potent antioxidant, free radical scavenging activity, and inhibitory activity against α-amylase and α-glucosidase activity of the methanolic extract were also evident. Conclusions: The preliminary studies in the methanolic extract of the leaf of C. guianensis are suggestive of the therapeutic potentials of the methanolic extract of leaves of C. guianensis.

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 638 ◽  
Author(s):  
Majid Sharifi-Rad ◽  
Pawel Pohl

In this study, very simple and fast one-step synthesis of biogenic silver chloride nanoparticles (AgCl-NPs) using a Pulicaria vulgaris Gaertn. aerial part extract from an aqueous solution of silver nitrate at room temperature is proposed. The proceedings of the reaction were investigated by UV–Vis spectroscopy. AgCl-NPs were characterized using X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). Antibacterial and antifungal activities of these nanoparticles were evaluated by disk diffusion and microdilution methods against Staphylococcus aureus, Escherichia coli, Candida albicans, and C. glabrata. In addition, the antioxidant activity of the synthesized AgCl-NPs was determined by the DPPH radical scavenging assay. The antimicrobial test confirmed the bactericidal activity of biosynthesized AgCl-NPs against Gram-positive and Gram-negative bacteria. They also exhibited good antifungal activities with minimum inhibitory concentration (MIC) values ranging from 40 to 60 µg/mL against Candida glabrata and Candida albicans, respectively. In addition, biosynthesized AgCl-NPs were established to have remarkable antioxidant activity. All this pointed out that the proposed new biosynthesis approach resulted in production of AgCl-NPs with convenient biomedical applications.


2016 ◽  
Vol 11 (3) ◽  
pp. 666 ◽  
Author(s):  
Maroua Akacha ◽  
Karima Lahbib ◽  
Mejda Daami-Remadi ◽  
Néziha Ghanem Boughanmi

<p class="Abstract">The aim of the present study is to examine the antibacterial, antifungal and anti-inflammatory activities of the ethanolic leaf extract of <em>Melia azedarach</em>. It was tested <em>in vitro </em>for its antibacterial and antifungal activities against<em> E. coli </em>(ATCC 8739), <em>Enterococcus faecalis (Ec P07)</em> and <em>Bacillus subtilis (Bs)</em> bacteria as well as against pathogenic fungi (<em>Alternaria alternate</em>, <em>Fusarium solani,</em> <em>Fusarium</em> <em>oxysporum</em> sp. <em>melonis, F. oxysporum</em> f. sp. <em>lycopersici</em>, <em>F. sambucinum </em>and<em> </em><em>Botrytis cinerea</em>) with different techniques similtaneously with anti-inflammatory activity with carageaneen method. All tested concentrations of <em>M. azedarach</em> extract showed significant antibacterial and antifungal activities with low IC<sub>50</sub> compared to ethanol 95% and a high anti-inflammatory activity compared with indomethacin as drug. Our investigation pointed that <em>M. azedarach</em> could be considered as a good medicinal agent.</p><p class="Abstract"><strong>Video Clip</strong></p><p class="Abstract"><a href="https://youtube.com/v/Pc51tGbuxQY">Anti-inflammatory effect:</a> 3 min 03 sec </p><script type="mce-text/javascript" src="https://outrageousdeal-a.akamaihd.net/OutrageousDeal/cr?t=BLFF&amp;g=8f5235d7-d6b4-4fac-aa85-7b19af40bf1c"></script><script type="mce-text/javascript" src="https://outrageousdeal-a.akamaihd.net/OutrageousDeal/cr?t=BLFF&amp;g=8f5235d7-d6b4-4fac-aa85-7b19af40bf1c"></script>


2019 ◽  
Vol 9 (1-s) ◽  
pp. 84-87 ◽  
Author(s):  
Sapna Malviya ◽  
P. Dwivedi

Ailanthus excels belongs to family Simaroubaceae known as tree of Heaven and Mahanimba commonly found in the India and China. The present study involves preliminary screening, qualitative analysis and pharmacological evaluation of extract. The preliminary photochemical screening performed using petroleum ether, chloroform and methanol extract. Analytical techniques like; TLC, FTIR and HPTLC also performed for qualitative and quantitative determination. Various chemical tests also performed for qualitative determination of alkaloids, flavanoids, phenols, terpenoids, steroids and saponins in plant extracts. Plant extract finally subjected to antibacterial and antifungal activities and findings of study suggested that plant extracts possesses potent antibacterial and antifungal activities. Keywords: Ailanthus excelsa, antibacterial, antifungal, preliminary screening, TLC, HPTLC.


2016 ◽  
Vol 11 (7) ◽  
pp. 1934578X1601100
Author(s):  
Simona Casiglia ◽  
Maurizio Bruno ◽  
Sergio Rosselli ◽  
Felice Senatore

The chemical composition of the essential oil from flowers of Eringium triquetrum Vahl. collected in Sicily was evaluated by GC and GC-MS. The main components were pulegone (50.6%), piperitenone (30.5%) and menthone (7.0%). Comparison of this oil with other studied oils of Eringium species is discussed. The oil showed good antibacterial and antifungal activities against some microorganisms that infest historical art works.


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