scholarly journals IN VITRO HEPATOPROTECTIVE ACTIVITY OF YELLOW LEAF EXTRACTS OF THESPESIA POPULNEA AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY

2019 ◽  
pp. 21-23
Author(s):  
UMADEVI A. ◽  
P. AJITH KUMAR
Keyword(s):  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Rat Hepatocytes ◽  
Hepatoprotective Activity ◽  
Yellow Leaf ◽  
Leaf Extracts ◽  
Isolated Rat Hepatocytes ◽  
Standard Drug ◽  
Serum Glutamate

Objective: The study was aimed to evaluate in vitro hepatoprotective activity of yellow leaf extracts of Thespesiapopulnea. Methods: Hepatoprotective activity is studied by carbon tetrachloride-induced hepato-toxicity in isolated rat hepatocytes. The biochemical parameters observed in serum were serum glutamate oxaloacetate transaminase (SGOT/AST), serum glutamate pyruvate transaminase (SGPT/ALT) levels. The extracts exhibited a dose-dependent reduction in AST, ALT levels. Results: Methanolic extract was found to exhibit higher hepatoprotection. T. populnea extract was found to be antihepatotoxic at a concentration of 125 mcg with a significant decrease in ALT (P<0.001) and AST (P<0.0001). Conclusion: The results suggest that the methanolic extract has produced significant (p<0.001) hepatoprotection by decreasing the activity of serum enzymes which is comparable to that of standard drug silymarin.

scholarly journals Study of hepatotoxins influence in vitro on basic biochemical indicators of liver functional state

2021 ◽  
pp. 15-18
Author(s):  
Liudmyla Maloshtan ◽  
Galyna Storozhenko ◽  
Liubov Galuzinska ◽  
Victoriia Fylymonenko ◽  
Omar Rashid Sadiq
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Classical Model ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Toxic Damage ◽  
Cell Homogenate ◽  
High Doses ◽  
Fluoroquinolone Group

An antimicrobial drug of the fluoroquinolone group, ciprofloxacin, is widely used in Ukraine. However, some researchers have noted the probable hepatotoxicity of this drug with prolonged use or use of high doses of ciprofloxacin. The aim of this study was to compare the effects of carbon tetrachloride, as a classical model of hepatocyte injury, with the effects of ciprofloxacin. The aim of the study was to investigate the biochemical parameters of the liver when simulating toxic damage to hepatocytes with carbon tetrachloride or ciprofloxacin. Materials and methods. The study was carried out on isolated rat hepatocytes, in whose culture medium carbon tetrachloride or ciprofloxacin was added. After incubation in the supernatant and cell homogenate, the activities of marker enzymes of cytolysis were determined: ALT, AST, γ-GTP, LF, LDH, DC and MDA. Results. The introduction of ciprofloxacin into the culture of hepatocytes at a concentration of LC50 caused changes in biochemical parameters similar to those caused by carbon tetrachloride. Thus, an increase in ALT, AST, γ-GTP, LF, LDH, DC and MDA was observed when CCl4 or ciprofloxacin was added to the culture. Conclusion. Incubation of rat hepatocytes with carbon tetrachloride or ciprofloxacin caused an increase in the level of enzymes and lipid peroxidation products. These parameters are indicators of gross changes in cells, which are the result of impaired keto acid formation, impaired redox reactions, impaired glycogen production

scholarly journals HEPATOPROTECTIVE ACTIVITY OF THE WHOLE PLANT OF NEPTUNIA PROSTRATA L. IN CARBON TETRACHLORIDE INDUCED RATS

2021 ◽  
pp. 56-59
Author(s):  
HONEY JAJO ◽  
RAJAT GHOSH
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Whole Plant ◽  
Layer Chromatography ◽  
Serum Glutamate

Objective: The aim of this study is to investigate the hepatoprotective activity of the whole plant of Neptunia Prostrata L. Methods: The whole plant was collected and identified as Neptunia Prostrata L. The collected plants were shade dried and pulverized to fine powdered of particle size (#) 40. It was then defatted with petroleum ether for 24 hour and soaked with methanol and ethanol, respectively. The extracts was filtered and distilled off using a rotary evaporator. The phytochemical screening of the extracts was carried out and thin layer chromatography study was also done. Acute toxicity study and in vivo hepatoprotective activity of the methanolic extract using CCL4 (carbon tetra chloride) induced model was investigated. Results: The phytochemical screening revealed the presence of alkaloids, glycosides (saponins), flavonoids, tannins, carbohydrates, proteins, phenolic, steroids and terpenoids. Thin-layer chromatography of the methanolic and ethanolic extracts with their fractions using different solvents were performed by taking petroleum ether and ethyl acetate (2:8) as mobile phase system and were able to observe the presence of many spots. Oral administration of methanolic extract of Neptunia prostrata at doses till 2000 mg/kg was found safe and shows good hepatoprotective activity by showing decreased levels of serum SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase) and ALP (alkaline phosphatase) when compared with the standard drug silymarin. Conclusion: The preliminary phytochemical screening of the methanol and ethanolic extract shows phytoconstituents such as flavonoids, triterpenoids, tannins, saponins, alkaloids and chromatographic studies indicates the presence of several components in varying abundance. The decrease of serum bilirubin level by the methanolic extract of the plant shows hepatoprotective activity. It has confirmed the traditional claim for its use in the treatment of jaundice.

Evaluation of Hepatoprotective and In-vitro Cytotoxic Activity of Leaves of Premna serratifolia Linn

2008 ◽  
Vol 1 (1) ◽  
pp. 145-152 ◽  
Author(s):  
Rajendran Vadivu ◽  
A. Jerad Suresh ◽  
K Girinath ◽  
P. Boopathi Kannan ◽  
R Vimala ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Hepatoprotective Activity ◽  
Alcoholic Extract ◽  
Standard Drug ◽  
Degree Of Protection ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Traditional Practitioners ◽  
Serum Glutamate

Premna serratifolia is used by the traditional practitioners as cardiotonic, antibiotic, anti-coagulant, stomachic, carminative, hepatoprotective, antitumor etc. The present study aims in the evaluation of hepatoprotective and in-vitro cytotoxic activity of alcoholic extract of leaves of Premna serratifolia Linn. Hepatoprotective activity is studied by carbon tetrachloride induced hepato-toxicity in rats and the in-vitro cytotoxic activity is carried out by tryphane blue exclusion method using EAC cell lines. The degree of protection in hepatoprotective activity has been measured by using biochemical parameters such as serum glutamate oxalate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin and total protein. The results suggest that the alcoholic extract at the dose level of 250mg/kg has produced significant  (p < 0.001) hepatoprotection by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation which is comparable to that of standard drug silymarin. The alcoholic extract also does exhibit the IC50 value of 75µg/ml which indicates the significant in-vitro cytotoxic activity of the extract. It is concluded that alcoholic extract of leaves of Premna serratifolia Linn is not only an effective hepatoprotective agent, but also possesses significant antitumor activity.  Keywords: Premna serratifolia Linn; Alcoholic extract hepatoprotective; In-vitro cytotoxic activity.  © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1046 

scholarly journals Evaluation of hepatoprotective effect of Waltheria indica against various NSAIDs-induced hepatic damage in rats

2017 ◽  
Vol 9 (1) ◽  
pp. 157 ◽  
Author(s):  
Amol Chandekar ◽  
Neeraj Upamanyu ◽  
Amber Vyas ◽  
Atul Tripathi ◽  
Surendra Agrawal ◽  
...  
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Traditional Medicine ◽  
Hepatic Fibrosis ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Blood Samples ◽  
Liver Disorders

<p>The objective of the present study was to evaluate methanolic extract of leaves of Waltheria indica linn. for hepatoprotective potency of the potent solvent extract. The hepatotoxicity was induced by diclofenac, carbon tetrachloride (CCl 4 ) and acetaminophen. In CCl 4 induced hepatotoxicity study, animals were divided into five groups (n=6). Methanolic extract of Waltheria indica (WIM) groups were injected in doses of 400 mg/kg and 600mg/kg body weight along with CCl 4 and Silymarin 100mg/kg was taken as standard drug. Similarly procedure was followed in diclofenac and acetaminophen induced hepatotoxicity. Blood samples and liver were collected and liver hisopathological studies were carried out. These histopathological analysis suggested that WIM extract have the ability to reduce the degree of hepatic fibrosis induced by various factors. And concluded that WIM extract has significant hepatoprotective activity thus this study scientifically support the theory to use of this plant in traditional medicine for the treatment of liver disorders.</p>

scholarly journals Hepatoprotective Potential of Mimusops elengi L leaf Extracts against Paracetamol Induced Hepatotoxicity in Rats

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
B. Edwin Jose ◽  
S. Manikandan ◽  
S. Jebaseelan ◽  
Dr.R. Meera ◽  
Dr.R. Kalirajan
Keyword(s):  
Aqueous Extract ◽  
Biochemical Parameters ◽  
Hepatoprotective Activity ◽  
Leaf Extracts ◽  
Standard Drug ◽  
Herbal Plants ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Mimusops Elengi ◽  
Serum Glutamate

Many traditional systems of medicines employ herbal drugs for the hepatoprotection. The aim of the study is to investigate the hepatoprotective activity of Mimusopselengi L leaf extracts extracts against paracetamol induced hepatotoxicity. However, herbal plants are the windfall for the humankind providing solution for most of the wellness breakdowns. Mimusopselengi L is one of such plants with enormous therapeutic and nutraceutical potencies. The main aspiration of the current investigation is to evaluate the hepatoprotective ability of methanolic and aqueous extract of Mimusopselengi L leaves against paracetamol induced hepatotoxicity using wistar rats through biochemical parameters and histopathological findings. The phytochemical screening was carried on the leaves extracts of Mimusopselengi L revealed the presence of some active ingredients such as Alkaloids, Tannins, Sponginess, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Mimusopselengi L was successively ethylacetate fraction with methanolic and aqueous extract against paracetamol (2 ml/kg.p.o) induced hepatotoxicity using Standard drug Liv 52 (5 ml/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP), serum bilirubin in paracetamol treated rats, which were restored towards normalization in Mimusopselengi L methanolic and aqueous extract (200 mg/kg and 200 mg/kg) treated animals. Thus, the present study ascertains that the leaf extract of Mimusopselengi L possesses significant hepatoprotective activity.

The Hep G2 Cell Line as a Possible Alternative to Isolated Hepatocytes in Cytotoxicity Studies

1988 ◽  
Vol 16 (1) ◽  
pp. 16-22
Author(s):  
Marina Marinovich ◽  
Jose L. Lorenzo ◽  
Liliana M. Flaminio ◽  
Agnese Granata ◽  
Corrado L. Galli
Keyword(s):  
Cell Line ◽  
Rat Hepatocytes ◽  
Prostaglandin E ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Isolated Rat Hepatocytes ◽  
G2 Cells ◽  
Isolated Rat

The hepatotoxicity of carbon tetrachloride (CC14) was evaluated in vitro in freshly isolated rat hepatocytes and in the human hepatoma cell line, Hep G2. Toxicity was assessed by the leakage of cytosolic enzymes (lactate dehydrogenase and aspartate aminotransferase) and cell viability (trypan blue exclusion). The established human cells were less sensitive to CCl4-induced injury; higher doses of the toxic agent and longer incubation times were necessary to elicit cell damage. Micromolar concentrations of prostaglandin E2 significantly decreased enzyme leakage in both Hep G2 cells and rat hepatocytes challenged with CC14; a stable derivative of prostacyclin (ZK 36374) was ineffective. These results suggest that human hepatoma Hep G2 cells may represent a valid alternative to isolated rat hepatocytes for an initial approach to the in vitro evaluation of cell toxicity.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

Acellular liver matrix improves the survival and functions of isolated rat hepatocytes cultured in vitro.

2004 ◽  
Author(s):  
Patrizia Burra ◽  
Silvia Tomat ◽  
Maria Teresa Conconi ◽  
Carlo Macchi ◽  
Francesco P Russo ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Hepatoprotective activity of Adina cordifolia against ethanol induce hepatotoxicity in rats

2012 ◽  
Vol 1 (9) ◽  
pp. 279-284 ◽  
Author(s):  
A Sharma ◽  
B Sangameswaran ◽  
V Jain ◽  
M S Saluja
Keyword(s):  
Pharmaceutical Journal ◽  
Hepatoprotective Activity ◽  
Serum Markers ◽  
Hepatocellular Injury ◽  
Standard Drug ◽  
Blood Samples ◽  
Hepatic Cells ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Histopathological Studies ◽  
Serum Glutamate

The acetone (AEAC) and aqueous extracts (AQEAC) of Adina cordifolia, belonging to the family Rubiaceae, were studied for hepatoprotective activity against Wister rats with liver damage induced by ethanol. It was found that AEAC and AQEAC, at a dose of 500 mg/kg body weight exhibited hepatoprotective effect by lowering the Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), alkaline phosphate and total bilirubin to a significant extent and also significantly increased the levels of total protein. The hepatoprotective activity was also supported by histopathological studies of liver tissue. Since results of biochemical studies of blood samples of ethanol treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by ethanol and blood samples from the animals treated with AEAC and AQEAC showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells against ethanol induced hepatocellular injury. The effects of AEAC and AQEAC were comparable with standard drug silymarin.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11619 International Current Pharmaceutical Journal 2012, 1(9): 279-284 

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