scholarly journals Evaluation of hepatoprotective effect of Waltheria indica against various NSAIDs-induced hepatic damage in rats

2017 ◽  
Vol 9 (1) ◽  
pp. 157 ◽  
Author(s):  
Amol Chandekar ◽  
Neeraj Upamanyu ◽  
Amber Vyas ◽  
Atul Tripathi ◽  
Surendra Agrawal ◽  
...  
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Traditional Medicine ◽  
Hepatic Fibrosis ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Blood Samples ◽  
Liver Disorders

<p>The objective of the present study was to evaluate methanolic extract of leaves of Waltheria indica linn. for hepatoprotective potency of the potent solvent extract. The hepatotoxicity was induced by diclofenac, carbon tetrachloride (CCl 4 ) and acetaminophen. In CCl 4 induced hepatotoxicity study, animals were divided into five groups (n=6). Methanolic extract of Waltheria indica (WIM) groups were injected in doses of 400 mg/kg and 600mg/kg body weight along with CCl 4 and Silymarin 100mg/kg was taken as standard drug. Similarly procedure was followed in diclofenac and acetaminophen induced hepatotoxicity. Blood samples and liver were collected and liver hisopathological studies were carried out. These histopathological analysis suggested that WIM extract have the ability to reduce the degree of hepatic fibrosis induced by various factors. And concluded that WIM extract has significant hepatoprotective activity thus this study scientifically support the theory to use of this plant in traditional medicine for the treatment of liver disorders.</p>

scholarly journals Evaluation of hepatoprotective potential of methanolic extract of the plant dolichos biflorus linn

2016 ◽  
Vol 2 (5) ◽  
pp. 116
Author(s):  
Deepthi B ◽  
Ashoka Shenoy M ◽  
Karunakar Hegde
Keyword(s):  
Body Weight ◽  
Liver Injury ◽  
Methanolic Extract ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Dolichos Biflorus ◽  
Liver Functions ◽  
Dose Dependent ◽  
Endogenous Enzymes

Present study is to evaluate the hepatoprotective potential of methanolic extract of the plant Dolichos biflorus Linn. against paracetamol and alcohol induced hepatotoxicity in rats. Oral administration of plant extract in two doses 200mg/kg and 400mg/kg body weight were subjected for the evaluation of hepatoprotective potential against alcohol (2ml/100g) and PCM (2g/kg) induced liver injury. Silymarin (25mg/kg) was used as a standard drug. The parameters like SGPT, SGO, ALP, TB and endogenous enzymes were estimated to assess the liver functions. In addition histopathological study was also carried out. Both the lower (200mg/kg) and higher dose (400mg/kg) of D.biflorus extract showed dose dependent significant decrease in SGPT, SGOT, ALP and TB levels when compared with toxic control. Both extracts showed decrease in LPO and increase in GSH, SOD and CAT levels. Hepatoprotective effect was also confirmed by histopathological analysis of liver which showed less damage in extract treated rats. The results obtained were comparable with that of the standard. The present study concluded that Dolichos biflorus Linn. plant were found to be effective against hepatotoxicity induced by Alcohol and Paracetamol.

scholarly journals ATTENUATION OF CARBON TETRACHLORIDE AND ETHANOL-INDUCED HEPATIC FIBROSIS IN RATS BY CALLIGONUM COMOSUM SHOOT EXTRACT

2017 ◽  
Vol 10 (3) ◽  
pp. 83
Author(s):  
Amria Mamdouh Mousa ◽  
Sherien Kamal Hassan ◽  
Nermin Mohammed El-sammad
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Matrix Metalloproteinase ◽  
Hepatic Fibrosis ◽  
Methanolic Extract ◽  
Elevated Serum ◽  
Matrix Metalloproteinase 2 ◽  
Ccl4 Administration ◽  
Liver And Kidney

ABSTRACTObjective: This study was designed to evaluate the hepatoprotective and antioxidant effects of methanolic extract of Calligonum comosum (C. comosum)shoots on the hepatic fibrosis induced by carbon tetrachloride and ethanol (CCl4/ethanol) in rats.Methods: A liver fibrosis was induced in Sprague Dawley rats by oral administration of CCl4 (1 ml/kg body weight, twice weekly for 10 weeks) alongwith ethanol (10% in drinking water 1 week before CCl4 administration and throughout the experiment). Rats were pretreated with C. comosumextract (daily, orally at a dose of 50 mg/kg body weight 1 week before CCl4 administration). At the end of the experiment, serum, and liver sampleswere subjected to biochemical investigations. In addition, liver and kidney tissues were evaluated for histopathological changes.Results: C. comosum extract pretreatment significantly reduced CCl4 - induced elevation in serum levels of aspartate aminotransaminase, alanineaminotransaminase, alkaline phosphatase, total bilirubin, urea, creatinine, and significantly elevated serum contents of total protein and albumin, aswell as an improvement in hepatic protein content, albumin/globulin ratio, body weight and relative liver and kidney weights. C. comosum extract alsosignificantly increased the hepatic levels of glucose-6-phosphatase, glutathione peroxidase, glutathione-S-transferase, catalase, superoxide dismutase,and glutathione with significant decrease in the contents of malondialdehyde and protein carbonyl. In addition, downregulation in expression ofthe fibrotic marker matrix metalloproteinase-2 was observed. C. comosum extract also ameliorated histopathological changes of CCl4/ethanol groupwhich further evidenced the biochemical findings.Conclusion: Our results confirmed that the methanolic extract of C. comosum shoots effectively protect against CCl4/ethanol-induced liver fibrosis,through its antioxidant property.Keywords: Calligonum comosum, Hepatic fibrosis, Carbon tetrachloride, Hepatoprotective effect, Antioxidants, Matrix metalloproteinase-2.

scholarly journals IN VITRO HEPATOPROTECTIVE ACTIVITY OF YELLOW LEAF EXTRACTS OF THESPESIA POPULNEA AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY

2019 ◽  
pp. 21-23
Author(s):  
UMADEVI A. ◽  
P. AJITH KUMAR
Keyword(s):  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Rat Hepatocytes ◽  
Hepatoprotective Activity ◽  
Yellow Leaf ◽  
Leaf Extracts ◽  
Isolated Rat Hepatocytes ◽  
Standard Drug ◽  
Serum Glutamate

Objective: The study was aimed to evaluate in vitro hepatoprotective activity of yellow leaf extracts of Thespesiapopulnea. Methods: Hepatoprotective activity is studied by carbon tetrachloride-induced hepato-toxicity in isolated rat hepatocytes. The biochemical parameters observed in serum were serum glutamate oxaloacetate transaminase (SGOT/AST), serum glutamate pyruvate transaminase (SGPT/ALT) levels. The extracts exhibited a dose-dependent reduction in AST, ALT levels. Results: Methanolic extract was found to exhibit higher hepatoprotection. T. populnea extract was found to be antihepatotoxic at a concentration of 125 mcg with a significant decrease in ALT (P<0.001) and AST (P<0.0001). Conclusion: The results suggest that the methanolic extract has produced significant (p<0.001) hepatoprotection by decreasing the activity of serum enzymes which is comparable to that of standard drug silymarin.

PHYTOCHEMICAL AND BIOLOGICAL STUDIES OF ROTULA AQUATICA LOUR. ROOTS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 34-38
Author(s):  
T. Shyam ◽  
◽  
S Ganapaty
Keyword(s):  
Body Weight ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Biological Studies ◽  
Bacteria And Fungi ◽  
Dose Levels ◽  
Dose Dependent ◽  
Chemical Evidence

Four compounds viz α-amyrin, β- amyrin, bauerenol and ellagic acid were isolated from the methanolic extract of Rotula aquatica roots. The structures of these compounds were elucidated on the basis of spectroscopic data analysis and chemical evidence. The extract was evaluated for hepatoprotective activity against carbon tetrachloride induced hepatotoxic model at a dose levels of 200,400 and 800 mg/ kg body weight and compared with that of the standard silymarin (25mg/kg body weight). It showed good hepatoprotective activity in a dose dependent manner. The extract was also screened for antimicrobial activity against various types of organisms like bacteria and fungi.

scholarly journals Hepatoprotective activity of Adina cordifolia against ethanol induce hepatotoxicity in rats

2012 ◽  
Vol 1 (9) ◽  
pp. 279-284 ◽  
Author(s):  
A Sharma ◽  
B Sangameswaran ◽  
V Jain ◽  
M S Saluja
Keyword(s):  
Pharmaceutical Journal ◽  
Hepatoprotective Activity ◽  
Serum Markers ◽  
Hepatocellular Injury ◽  
Standard Drug ◽  
Blood Samples ◽  
Hepatic Cells ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Histopathological Studies ◽  
Serum Glutamate

The acetone (AEAC) and aqueous extracts (AQEAC) of Adina cordifolia, belonging to the family Rubiaceae, were studied for hepatoprotective activity against Wister rats with liver damage induced by ethanol. It was found that AEAC and AQEAC, at a dose of 500 mg/kg body weight exhibited hepatoprotective effect by lowering the Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), alkaline phosphate and total bilirubin to a significant extent and also significantly increased the levels of total protein. The hepatoprotective activity was also supported by histopathological studies of liver tissue. Since results of biochemical studies of blood samples of ethanol treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by ethanol and blood samples from the animals treated with AEAC and AQEAC showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells against ethanol induced hepatocellular injury. The effects of AEAC and AQEAC were comparable with standard drug silymarin.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11619 International Current Pharmaceutical Journal 2012, 1(9): 279-284 

Evaluation of Hepatoprotective activity of Methanol extract of Persicaria hydropiper: An in vivo screening

2021 ◽  
pp. 5819-5824
Author(s):  
Pooja Kamra ◽  
Mahaveer Singh ◽  
Hardarshan Singh Lamba ◽  
Birendra Srivastava
Keyword(s):  
Carbon Tetrachloride ◽  
Intraperitoneal Administration ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Standard Drug ◽  
Whole Plant ◽  
Dose Dependent ◽  
Histopathological Investigation ◽  
Different Doses

The present study aimed to evaluate the hepatoprotective potential of methanolic whole plant extract of Persicaria hydropiper in carbon tetrachloride (CCl4) induced hepatotoxicity model. Hepatotoxicity was induced in rats by intraperitoneal administration of carbon tetrachloride (CCl4) for seven days. The extract was thereafter administered at two different doses of 200 mg/kg and 400 mg/kg body weight for next seven days. Silymarin was used as a reference standard. The extract revealed hepatoprotective activity in dose dependent manner. The dose of 400 mg/kg exhibited maximum hepatoprotective ability as apparent from several evaluation parameters including liver function profile, bilirubin, antioxidant enzymes as well as histopathological investigation which was comparable to the standard drug Silymarin respectively. These findings sustenance the use of the extract as an adjuvant with existing therapy for treatment of liver ailments.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF THE WHOLE PLANT OF NEPTUNIA PROSTRATA L. IN CARBON TETRACHLORIDE INDUCED RATS

2021 ◽  
pp. 56-59
Author(s):  
HONEY JAJO ◽  
RAJAT GHOSH
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Whole Plant ◽  
Layer Chromatography ◽  
Serum Glutamate

Objective: The aim of this study is to investigate the hepatoprotective activity of the whole plant of Neptunia Prostrata L. Methods: The whole plant was collected and identified as Neptunia Prostrata L. The collected plants were shade dried and pulverized to fine powdered of particle size (#) 40. It was then defatted with petroleum ether for 24 hour and soaked with methanol and ethanol, respectively. The extracts was filtered and distilled off using a rotary evaporator. The phytochemical screening of the extracts was carried out and thin layer chromatography study was also done. Acute toxicity study and in vivo hepatoprotective activity of the methanolic extract using CCL4 (carbon tetra chloride) induced model was investigated. Results: The phytochemical screening revealed the presence of alkaloids, glycosides (saponins), flavonoids, tannins, carbohydrates, proteins, phenolic, steroids and terpenoids. Thin-layer chromatography of the methanolic and ethanolic extracts with their fractions using different solvents were performed by taking petroleum ether and ethyl acetate (2:8) as mobile phase system and were able to observe the presence of many spots. Oral administration of methanolic extract of Neptunia prostrata at doses till 2000 mg/kg was found safe and shows good hepatoprotective activity by showing decreased levels of serum SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase) and ALP (alkaline phosphatase) when compared with the standard drug silymarin. Conclusion: The preliminary phytochemical screening of the methanol and ethanolic extract shows phytoconstituents such as flavonoids, triterpenoids, tannins, saponins, alkaloids and chromatographic studies indicates the presence of several components in varying abundance. The decrease of serum bilirubin level by the methanolic extract of the plant shows hepatoprotective activity. It has confirmed the traditional claim for its use in the treatment of jaundice.

scholarly journals Membrane Stabilizing and Preliminary Hypoglycemic Activities of Picrasma javanica

2013 ◽  
Vol 16 (1) ◽  
pp. 89-92
Author(s):  
Tasnuva Sharmin ◽  
Farhana Islam ◽  
Md. Al Amin Sikder ◽  
Shaila Kabir ◽  
Mohammad Rashedul Haque ◽  
...  
Keyword(s):  
Body Weight ◽  
Salicylic Acid ◽  
Carbon Tetrachloride ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Methanol Extract ◽  
Pharmaceutical Journal ◽  
Hypotonic Solution ◽  
Standard Drug ◽  
Crude Methanol Extract

The methanol extract of leaves of Picrasma javanica Blume and its n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were evaluated for membrane stabilizing and preliminary hypoglycemic activities. In hypotonic solution and heat-induced conditions, the crude methanol extract inhibited 65.30±0.52% and 36.79±0.38% haemolysis of RBC as compared to 72.79% and 42.12% produced by standard drug, acetyl salicylic acid, respectively (0.10 mg/ml). The crude extract also showed blood sugar lowering efficacy at 200 and 400 mg/ kg body weight, with 48.63% reduction of blood sugar level at 400 mg/kg body weight while the standard drug, Glibenclamide (10 mg/kg body weight) demonstrated 53.7% reduction of blood sugar level at 3rd hour of administration. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14500 Bangladesh Pharmaceutical Journal 16(1): 89-92, 2013

scholarly journals Antidiarrhoeal activity of rind of Punica granatum

2013 ◽  
Vol 2 (5) ◽  
pp. 101-104 ◽  
Author(s):  
Saleha Akter ◽  
Amit Sarker ◽  
Md. Sanowar Hossain
Keyword(s):  
Body Weight ◽  
Animal Models ◽  
Magnesium Sulfate ◽  
Castor Oil ◽  
Methanolic Extract ◽  
Punica Granatum ◽  
Pharmaceutical Journal ◽  
Phytochemical Screening ◽  
Traditional Use ◽  
Standard Drug

Punica granatum (Family: Punicaceae) is a widely consumed fruit in Bangladesh locally known as Anar or Bedana. Traditionally the plant is used to treat diarrhoea, dysentery, hemorrhage, tuberculosis and chronic periodontitis. The present study was undertaken in order to justify and authenticate the traditional use of rind of P. granatum fruit in diarrhoea. Methanolic extract of the rind of the fruit was tested in castor oil- and magnesium sulfate-induced diarrhoea in mice at the doses of 200 and 400 mg/kg body weight. The results of the present study revealed significant antidiarrhoeal activity (p<0.001) in both animal models. The extract inhibited 31.25% defaecation at the dose of 200 mg/kg and 53.75% at the dose of 400 mg/kg in castor oil-induced diarrhoea while standard drug loperamide inhibited 71.25% defaecation. In magnesium sulfate-induced diarrhoea the inhibition of defaecation was 45.71 and 57.14% at the doses of 200 and 400 mg/kg respectively while loperamide inhibited 64.29% defaecation. The antidiarrhoeal effect of the extract was concentration dependent in both castor oil-induced diarrhoea and magnesium sulfate-induced diarrhoea. Phytochemical screening of the extract revealed the presence of flavonoids and alkaloids that may play key role in its antidiarrhoeal activity. The results of the present study validate the folkloric use of the rind of P. granatum in the treatment of diarrhoea.DOI: http://dx.doi.org/10.3329/icpj.v2i5.14437 International Current Pharmaceutical Journal, April 2013, 2(5): 101-104

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