scholarly journals SCREENING FOR PHYTOCHEMICALS AND FTIR ANALYSIS OF MYRISTICA DACTYLOIDS FRUIT EXTRACTS

2016 ◽  
Vol 9 (1) ◽  
pp. 315 ◽  
Author(s):  
P. Rajiv ◽  
A. Deepa ◽  
P. Vanathi ◽  
D. Vidhya
Keyword(s):  
Fourier Transform ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Spectroscopy Analysis ◽  
Standard Methods ◽  
Fruit Extracts ◽  
Ft Ir ◽  
Amine Groups ◽  
Ir Analysis

<p><strong>Objective: </strong>The present investigation focus on screening of phytochemicals and FT-IR analysis of <em>Myristica dactyloids </em>fruit extracts. The fruit extracts were prepared using five different solvents.</p><p><strong>Methods: </strong>The phytochemical analysis and FT-IR (Fourier transform infrared spectroscopy) analysis were performed using standard methods.</p><p><strong>Results: </strong>The results reveals that the alkaloids, steroids, flavonoids, phenolic compounds, proteins, carbohydrates, cardio glycosides and saponins were present in methanolic extract when compared to other solvent extracts. FT-IR analysis shows the presence of different functional groups such as carboxylic acids, aromatics, alkanes, alcohols, phenols, aliphatic amines, alkenes and amine groups in the fruit extracts.</p><p><strong>Conclusion: </strong>The study concluded that the methanolic extract (<em>M. dactyloides </em>fruit) has potential bioactive compounds.</p>

scholarly journals Preliminary Phytochemical Analysis and Spectroscopic Analysis Methanolic Extract of Asteracantha Longifolia (Nees.)

2021 ◽  
pp. 80-87
Author(s):  
D. Veerakumar ◽  
M. Muthulingam
Keyword(s):  
Spectral Analysis ◽  
Fourier Transform ◽  
Spectroscopic Analysis ◽  
Methanolic Extract ◽  
Nitrogen Compounds ◽  
Phytochemical Analysis ◽  
Secondary Alcohols ◽  
Infrared Spectral ◽  
Ft Ir ◽  
Study Offer

The present investigation was focused on the preliminary phytochemical, UV-VIS spectrum and Fourier Transform Infrared Spectral analysis of Asteracantha longifolia. The Methanolic extract from the leaves of Asteracantha longifolia  were tested for the availability of alkaloids, flavonoids, tannins, saponins, proteins, Terpenoids, Phenols, Steroids, Glycosides and Carbohydrates. The UV-VIS profile demonstrated various peaks ranging from 400-700 nm with various Extract respectively. The FT-IR spectrum confirmed the presence of secondary alcohols, alkanes, alkynes, aromatics, alkenes, sulphur, phosphorus, esters, hydrocarbons, nitrogen compounds (amines) in methanolic extract. The results of this study offer a platform of using Asteracantha longifolia leaves as herbal alternative for various diseases including diabetic, cardiovascular etc. The results of this study offer a platform of using Asteracantha longifolia leaves as herbal alternative for various diseases including cancer and diabetic.

scholarly journals Extraction of Dyes from Sunflower Petal and Their Fourier Transform Infrared Characterization

2022 ◽  
Vol 3 (2) ◽  
Author(s):  
Gboyega Oluwaseun Oyeleke ◽  
Ibraheem Abimbade Abdulazeez ◽  
Ajisola Agnes Adebisi ◽  
Kehinde Nasiru Oyekanmi ◽  
Segun Olaitan Akinbode
Keyword(s):  
Fourier Transform ◽  
Helianthus Annuus ◽  
Functional Groups ◽  
Methanolic Extract ◽  
Fourier Transform Infrared ◽  
Infrared Spectrometry ◽  
Fourier Transform Infrared Spectrometry ◽  
Naoh Solution ◽  
Ft Ir ◽  
Ir Analysis

Three solvents of different polarities (water, methanol and 1% NaOHsolution) were used to extract dyes that produced different shades fromdried sunflower (Helianthus annuus) petal. The extraction proceduresusing different solvent types were carried out separately. The dye extractswere thereafter subjected to Fourier Transform Infrared Spectrometry(FT-IR) analysis for characterization in terms of functional groups. Theintensities of the extracted dyes on the shade of colours obtained on piecesof cotton material varied from yellow in methanolic extract to light yellowin aqueous and black in 1% NaOH solution extracts. The results obtainedfrom the FT-IR analysis revealed the presence of several useful functionalgroups such as N-H, C=H, O-H and C=O in the extracts.

scholarly journals QUALITATIVE PHYTOCHEMICAL SCREENING AND FTIR SPECTROSCOPIC ANALYSIS OF GREWIA TILIFOLIA (VAHL) LEAF EXTRACTS

2019 ◽  
pp. 100-107
Author(s):  
DHARMASOTH RAMA DEVI ◽  
GANGA RAO BATTU
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Functional Groups ◽  
Fourier Transform Infrared ◽  
Primary Amines ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Ft Ir ◽  
Ir Analysis

Objective: The present study aim is to analyse the phytochemicals present in Grewia tilifolia (Vahl) leaf extracts by using Qualitative phytochemical analysis, Fourier-transform infrared spectroscopy (FTIR). Methods: The leaf extracts were prepared using eight different solvents. The phytochemical analysis Fourier transform infrared spectroscopy (FT-IR) analysis were performed using standard methods. Results: The FTIR spectroscopic studies revealed different characteristic peak values with various functional groups present in the compounds of respective extracts. The FT-IR analysis shows the presence of different functional groups such as alcohols, phenols, alkanes, carboxylic acids, aldehydes, ketones, alkenes, primary amines, aromatics, esters, ethers, alkyl halides and aliphatic amine compounds, which showed major compounds present in the leaf extracts. The present study generated the FTIR spectrum profile for the medicinally important plant Grewia tilifolia. Conclusion: The present study provides evediences that different extracts of Grewia tilifolia leaf is useful to cure many serious diseases which remained still problematic and for further isolation of bioactive compounds from the plant which could be of interest for the development of the new drug.

scholarly journals Application of Fourier Transform Infrared Spectroscopy and Chemometrics for Differentiation of Salmonella enterica Serovar Enteritidis Phage Types

2010 ◽  
Vol 76 (11) ◽  
pp. 3538-3544 ◽  
Author(s):  
Ornella Preisner ◽  
Raquel Guiomar ◽  
Jorge Machado ◽  
Jos� Cardoso Menezes ◽  
Jo�o Almeida Lopes
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Salmonella Enterica ◽  
Intact Cell ◽  
Phage Type ◽  
Fourier Transform Infrared ◽  
Intact Cells ◽  
Phage Types ◽  
Ft Ir ◽  
Ir Analysis

ABSTRACT Fourier transform infrared (FT-IR) spectroscopy and chemometric techniques were used to discriminate five closely related Salmonella enterica serotype Enteritidis phage types, phage type 1 (PT1), PT1b, PT4b, PT6, and PT6a. Intact cells and outer membrane protein (OMP) extracts from bacterial cell membranes were subjected to FT-IR analysis in transmittance mode. Spectra were collected over a wavenumber range from 4,000 to 600 cm−1. Partial least-squares discriminant analysis (PLS-DA) was used to develop calibration models based on preprocessed FT-IR spectra. The analysis based on OMP extracts provided greater separation between the Salmonella Enteritidis PT1-PT1b, PT4b, and PT6-PT6a groups than the intact cell analysis. When these three phage type groups were considered, the method based on OMP extract FT-IR spectra was 100% accurate. Moreover, complementary local models that considered only the PT1-PT1b and PT6-PT6a groups were developed, and the level of discrimination increased. PT1 and PT1b isolates were differentiated successfully with the local model using the entire OMP extract spectrum (98.3% correct predictions), whereas the accuracy of discrimination between PT6 and PT6a isolates was 86.0%. Isolates belonging to different phage types (PT19, PT20, and PT21) were used with the model to test its robustness. For the first time it was demonstrated that FT-IR analysis of OMP extracts can be used for construction of robust models that allow fast and accurate discrimination of different Salmonella Enteritidis phage types.

scholarly journals Chemical constituents of Algerian mandarin (Citrus reticulata) essential oil by GC-MS and FT-IR analysis

2020 ◽  
Vol 33 (4) ◽  
pp. 197-201
Author(s):  
Hicham Boughendjioua ◽  
Nour El Houda Mezedjeri ◽  
Ilhem Idjouadiene
Keyword(s):  
Essential Oil ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Complex Mixture ◽  
Citrus Reticulata ◽  
Qualitative And Quantitative Analysis ◽  
Spectroscopy Analysis ◽  
Qualitative And Quantitative ◽  
Ft Ir ◽  
Ir Analysis

Abstract Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Citrus oils are a complex mixture of more than a hundred components of differing chemical natures. Qualitative and quantitative analysis by gas chromatography coupled with mass spectrometry (GCMS) of the Citrus reticulata essential oil collected from El Hadaïk, Skikda City (Algeria), identified 28 compounds representing a total of 99.41%. The essential oil is constituted mainly of: D-Limonene (85.10%), Sabinene (2.49%), Linalyl acetate (2.00%), Copaene (1.80%) et α-Pinene (1.75%) totaling approximately 93.14%. The essential oil was also analyzed by Fourier transform infrared spectroscopy analysis (FTIR). FTIR spectroscopy allowed us to identify 10 volatile compounds and indicated that the functional groups of the essential oils are C-H (Alkene), C-H (aromatic) and C=C. The obtained results have shown that the essential oil can be fully utilized for pharmacy, cosmetology and industry.

scholarly journals Phytochemical screening and in-vitro antibacterial and DPPH free radical scavenging activities of methanol extract of root of Combretum album Pers.

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Functional Groups ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Chloroform Fraction ◽  
Active Ingredients ◽  
Ic50 Value ◽  
Ft Ir ◽  
Ir Analysis

The objective of the study was to investigate in-vitro antibacterial and antioxidant activities of methanolic extract of Combretum album Pers. root. In brief antibacterial efficacies of methanol extract and its petroleum ether fraction (MePET), chloroform fraction (MeCH), ethyl acetate fraction (MeEA) and water fraction (MeAQ) were determined by agar well diffusion assay along with Minimum Inhibitory Concentrations (MICs). In-vitro antioxidant efficacies were evaluated by DPPH radical scavenging method. Preliminary phytochemical assay, Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy analyses were employed to detect the plausible active ingredients. Methanol extract showed broad spectrum antibacterial activity having highest inhibition zone against Staphylococcus aureus MTCC 2940 (21.67±0.58 mm) and MICs ranged from100 µg/ml to 250 µg/ml. The scavenging activity of methanol extract of root of C. album was concentration dependant and IC50 value was 136.08 µg/ml. The lowest MIC (5 µg/ml) was noted with MeCH against B. subtilis (MTCC 441). MeCH showed highest antioxidant activity with an IC50 value of 12.98 ?g/mL and MePET, MeEA, MeAQ and Ascorbic acid presented antioxidant potential with IC50 values of 16.10, 15.07, 17.44 and 13.40??g/mL respectively. Preliminary phytochemical tests and FT-IR analysis revealed presence of various phytochemicals and functional groups like hydroxyl, carboxylic acids, amidines, amines, aromatics and esters. Three compounds were elucidated from bioactive TLC fraction of MeCH in GC-MS analysis supported by presence of various functional groups in MeCH as detected in FT-IR analysis. It is concluded that methanolic extract of C. album root is a potential antibacterial and antioxidant agent where presence of N-[5-[4-pyridinyl)-1H-1,2,4-triazol-3-yl]-benzamide (compound 1), pyruvic acid (compound 2) and methyloacetone (compound 3) are responsible as possible active ingredients.

scholarly journals ANTIMICROBIAL ACTIVITY OF LEMON PEEL (CITRUS LIMON) EXTRACT

2017 ◽  
Vol 9 (4) ◽  
pp. 79 ◽  
Author(s):  
Junab Ali ◽  
Biswajit Das ◽  
Trideep Saikia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Citrus Limon ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Standard Methods ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) in conjugation with phytochemical analysis.Methods: The methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) was separated from fruits, shade dried, powdered and extracted using methanol, analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is Gram-positive (Staphylococcus aureus) and other is Gram-negative (Escherichia coli) and 1 fungal strains (Candida albicans) using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the methanolic extract of the fruits peel Citrus limon. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides and citric acids and tannins. The antimicrobial activity of the methanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that methanolic extract of the peel of Citrus Limon contains the high presence of phytochemicals. The methanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards. 

Phytochemical Screening, GC-MS, FT-IR Analysis of Methanolic Extract of Curcuma caesia Roxb (Black Turmeric)

2017 ◽  
Vol 9 (6) ◽  
pp. 952-956 ◽  
Author(s):  
Muthukumaran Pakkirisamy ◽  
Suresh Kumar Kalakandan ◽  
Karthikeyen Ravichandran
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Ft Ir ◽  
Ir Analysis

scholarly journals Calcium-Induced Activity and Folding of a Repeat in Toxin Lipase from Antarctic Pseudomonas fluorescens Strain AMS8

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 27 ◽  
Author(s):  
Nur Shidaa Mohd Ali ◽  
Abu Bakar Salleh ◽  
Raja Noor Zaliha Raja Abd Rahman ◽  
Thean Chor Leow ◽  
Mohd Shukuri Mohamad Ali
Keyword(s):  
Fourier Transform ◽  
Secondary Structure ◽  
Pseudomonas Fluorescens ◽  
Calcium Binding ◽  
Protein Structures ◽  
Titration Calorimetry ◽  
Ftir Analysis ◽  
High Concentration ◽  
Spectroscopy Analysis ◽  
Repeat Structure

It is hypothesized that the Ca2+ ions were involved in the activity, folding and stabilization of many protein structures. Many of these proteins contain repeat in toxin (RTX) motifs. AMS8 lipase from Antarctic Pseudomonas fluorescens strain AMS8 was found to have three RTX motifs. So, this research aimed to examine the influence of Ca2+ ion towards the activity and folding of AMS8 lipase through various biophysical characterizations. The results showed that CaCl2 increased lipase activity. The far-UV circular dichroism (CD) and Fourier-transform infrared (FTIR) analysis suggested that the secondary structure content was improved with the addition of CaCl2. Fluorescence spectroscopy analysis showed that the presence of CaCl2 increased protein folding and compactness. Dynamic light scattering (DLS) analysis suggested that AMS8 lipase became aggregated at a high concentration of CaCl2.The binding constant (Kd) value from the isothermal titration calorimetry (ITC) analysis proved that the Ca2+ ion was tightly bound to the AMS8 lipase. In conclusion, Ca2+ ions play crucial roles in the activity and folding of the AMS8 lipase. Calcium binding to RTX nonapeptide repeats sequences will induced the formation and folding of the RTX parallel β-roll motif repeat structure.

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