NEPHRO-PROTECTIVE ACTIVITY OF ISOLATED METHANOL FRACTIONS PHYTO-COMPOUND FROM BARK OF TERMINALIA ARJUNA

Objective: The aim of this study was to evaluate the antioxidant property of the isolated phytocompounds from TA (Terminalia arjuna) bark and in vivo study for nephro-protective and oxidative stress reducing activity in experimentally induced albino male rats.Methods: Fractions from methanol crude TA extract were collected by column chromatography and F27, F28, F29 fractions were selected on the basis of antioxidant property by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The in vivo study performed by 30 albino male rats which were randomly divided into five groups: Group I (control)were taken normal food and water, Groups II (uremic) were injected acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d, Group III, IV and V(extract treatment) acetaminophen intraperitoneally at the dose of 500 mg/kg/d for 10 d with co-administered orally of methanol fraction F27, F28, F29 at the dose of 100 mg/kg/d for 15 d respectively.Results: After scarification of rats, the uremic marker plasma urea (80%), creatinine (85%) were elevated and antioxidant enzyme marker such as plasma SOD and catalase level were significantly increased (p<0.05)in Group IV compared to Group II. The total phenolic content of the F28 methanolic fraction was (815.48±8.11) mg gallic acid equivalent/g of extract. For isolation of available compound by 1H NMR study in F28 methanol fraction of TA bark was arjunoside IV which contained olefinic proton (a pair of carbon atom linked with double bond).Conclusion: Among the three methanolic fraction of TA bark, F28 was shown best antioxidative, nephron-protective and oxidative stress reducing property.

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Hearing, reactive metabolite formation, and oxidative stress in cochleae after a single acute overdose of acetaminophen: an in vivo study

Toxicology Mechanisms and Methods ◽

10.3109/15376516.2015.1122136 ◽

2016 ◽

Vol 26 (2) ◽

pp. 104-111 ◽

Cited By ~ 5

Author(s):

Mitchell R. McGill ◽

Stefanie Kennon-McGill ◽

Dianne Durham ◽

Hartmut Jaeschke

Keyword(s):

Oxidative Stress ◽

In Vivo Study ◽

Reactive Metabolite ◽

Metabolite Formation ◽

Acute Overdose ◽

And Oxidative Stress

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Evaluation of anti-oxidant status in-vitro and in-vivo in hydro-alcoholic extract of Eugenia caryophyllus

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v4i1.5880 ◽

2016 ◽

Vol 4 (1) ◽

pp. 19

Author(s):

Roma Ghai ◽

Kandasamy Nagarajan ◽

Jitendra Singh ◽

Shiwam Swarup ◽

Minu Keshari

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Antioxidant Property ◽

Total Phenolic ◽

Alcoholic Extract ◽

Flavonoid Content ◽

Anti Oxidant ◽

Oxidant Status

Free radicals mediated oxidative stress is the major risk factor for many chronic diseases like atherosclerosis, diabetes mellitus, arthritis, cancer, ageing and neurodegenerative diseases. Therapy with anti-oxidants is gradually gaining lot of importance for treatment of such diseases. Hydro-alcoholic extract of Eugenia caryophyllus was studied for its in-vivo antioxidant activity using two different animal models viz. Triton induced hyperlipidemia and High fat diet induced hyperlipidemia. Total phenolic content and total flavonoid content, DPPH assay was also carried out for in vitro anti-oxidant efficacy. Total protein, lipid peroxidation (MDA), reduced glutathione, superoxide dismutase and catalase were evaluated in the liver tissue in Triton induced hyperlipidemia and diet induced hyperlipidemia models. The study findings indicated significant in-vivo and in-vitro antioxidant property that may be related to the amount of polyphenols and flavonoids present in the extract. These results clearly indicate that Eugenia caryophyllus is effective against free radical mediated oxidative stress.

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In vivo Study of Antiplasmodial Activity of Terminalia avicennioides and Its Effect on Lipid Profile and Oxidative Stress in Mice Infected with Plasmodium berghei

British Microbiology Research Journal ◽

10.9734/bmrj/2013/3680 ◽

2013 ◽

Vol 3 (4) ◽

pp. 501-512 ◽

Cited By ~ 1

Author(s):

Olusegun Akanbi

Keyword(s):

Oxidative Stress ◽

Lipid Profile ◽

Plasmodium Berghei ◽

Antiplasmodial Activity ◽

In Vivo Study ◽

And Oxidative Stress

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Effects of Red Fruit (Pandanus Conoideus Lam) Oil on Exercise Endurance and Oxidative Stress in Rats at Maximal Physical Activity

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2020.3428 ◽

2020 ◽

Vol 8 (A) ◽

pp. 164-169

Author(s):

FAJAR APOLLO SINAGA ◽

Pangondian Hotliber Purba ◽

Rika Nailuvar Sinaga ◽

Ramlan Silaban

Keyword(s):

Oxidative Stress ◽

Physical Activity ◽

Free Radicals ◽

Tissue Injury ◽

Male Rats ◽

Control Group ◽

Group I ◽

Exercise Endurance ◽

Fruit Oil ◽

And Oxidative Stress

BACKGROUND: Living cells continuously produce free radicals and reactive oxygen species (ROS) as a part of metabolic processes. These free radicals are neutralized by an antioxidant defense system. Maximal physical activity can produce an imbalance between ROS and antioxidants and thus may cause oxidative stress, which is possibly related to fatigue and tissue injury. Red fruit oil contains high beta-carotene and tocopherol as antioxidants which could overcome oxidative stress condition. AIM: The aim of this study was to determine the effect of red fruit oil on exercise endurance and oxidative stress in rats at a maximal physical activity. METHODS: Twenty-four male rats were divided into four groups. The control group (I) was administered with 1.5 ml distilled water, intervention groups (II), (III), and (IV) were administered with different doses of red fruit oil (0.15 ml/kgBW, 0.3 ml/kgBW, and 0.6 ml/kgBW, respectively). All groups were trained to swim for 4 weeks and then were forced to swim without a load until being exhausted. The malondialdehyde (MDA), glutathione peroxidase (GPx) levels, and time of swimming to exhaustion were measured in all groups. RESULT: The results showed that MDA level obtained was decreasing significantly (p < 0.05), GPx and time of swimming were increasing significantly (p < 0.05) in the intervention groups. CONCLUSION: The results suggest that red fruit oil can obviously reduce MDA level, increased GPx, and endurance and delay fatigue induced by maximal physical activity in the rat.

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Canthin-6-one ameliorates TNBS-induced colitis in rats by modulating inflammation and oxidative stress. An in vivo and in silico approach

Biochemical Pharmacology ◽

10.1016/j.bcp.2021.114490 ◽

2021 ◽

Vol 186 ◽

pp. 114490

Author(s):

Karuppusamy Arunachalam ◽

Amilcar Sabino Damazo ◽

Antonio Macho ◽

Monica Steffi Matchado ◽

Eduarda Pavan ◽

...

Keyword(s):

Oxidative Stress ◽

In Silico ◽

And Oxidative Stress

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Impact of Polyphenolic-Food on Longevity: An Elixir of Life. An Overview

Antioxidants ◽

10.3390/antiox10040507 ◽

2021 ◽

Vol 10 (4) ◽

pp. 507

Author(s):

Rosaria Meccariello ◽

Stefania D’Angelo

Keyword(s):

Oxidative Stress ◽

Food Sources ◽

Preventive Action ◽

Nutritional Interventions ◽

Beneficial Effects ◽

Age Related ◽

Macromolecular Damage ◽

And Oxidative Stress

Aging and, particularly, the onset of age-related diseases are associated with tissue dysfunction and macromolecular damage, some of which can be attributed to accumulation of oxidative damage. Recently, growing interest has emerged on the beneficial effects of plant-based diets for the prevention of chronic diseases including obesity, diabetes, and cardiovascular disease. Several studies collectively suggests that the intake of polyphenols and their major food sources may exert beneficial effects on improving insulin resistance and related diabetes risk factors, such as inflammation and oxidative stress. They are the most abundant antioxidants in the diet, and their intake has been associated with a reduced aging in humans. Polyphenolic intake has been shown to be effective at ameliorating several age-related phenotypes, including oxidative stress, inflammation, impaired proteostasis, and cellular senescence, both in vitro and in vivo. In this paper, effects of these phytochemicals (either pure forms or polyphenolic-food) are reviewed and summarized according to affected cellular signaling pathways. Finally, the effectiveness of the anti-aging preventive action of nutritional interventions based on diets rich in polyphenolic food, such as the diets of the Blue zones, are discussed.

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Fetuin-A exerts a protective effect against experimentally induced intestinal ischemia/reperfusion by suppressing autophagic cell death

Experimental Biology and Medicine ◽

10.1177/1535370221995207 ◽

2021 ◽

pp. 153537022199520

Author(s):

Nanees F El-Malkey ◽

Amira E Alsemeh ◽

Wesam MR Ashour ◽

Nancy H Hassan ◽

Husam M Edrees

Keyword(s):

Oxidative Stress ◽

Rat Model ◽

Intestinal Ischemia ◽

Ischemia Reperfusion ◽

Protective Role ◽

Beclin 1 ◽

Male Rats ◽

Fetuin A ◽

Intestinal Ischemia Reperfusion ◽

And Oxidative Stress

Intestinal tissue is highly susceptible to ischemia/reperfusion injury in many hazardous health conditions. The anti-inflammatory and antioxidant glycoprotein fetuin-A showed efficacy in cerebral ischemic injury; however, its protective role against intestinal ischemia/reperfusion remains elusive. Therefore, this study investigated the protective role of fetuin-A supplementation against intestinal structural changes and dysfunction in a rat model of intestinal ischemia/reperfusion. We equally divided 72 male rats into control, sham, ischemia/reperfusion, and fetuin-A-pretreated ischemia/reperfusion (100 mg/kg/day fetuin-A intraperitoneally for three days prior to surgery and a third dose 1 h prior to the experiment) groups. After 2 h of reperfusion, the jejunum was dissected and examined for spontaneous contractility. A jejunal homogenate was used to assess inflammatory and oxidative stress enzymes. Staining of histological sections was carried out with hematoxylin, eosin and Masson’s trichrome stain for evaluation. Immunohistochemistry was performed to detect autophagy proteins beclin-1, LC3, and p62. This study found that fetuin-A significantly improved ischemia/reperfusion-induced mucosal injury by reducing the percentage of areas of collagen deposition, increasing the amplitude of spontaneous contraction, decreasing inflammation and oxidative stress, and upregulating p62 expression, which was accompanied by beclin-1 and LC3 downregulation. Our findings suggest that fetuin-A treatment can prevent ischemia/reperfusion-induced jejunal structural and functional changes by increasing antioxidant activity and regulating autophagy disturbances observed in the ischemia/reperfusion rat model. Furthermore, fetuin-A may provide a protective influence against intestinal ischemia/reperfusion complications.

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