scholarly journals EFFICACY OF WATTAKAKA VOLUBILIS AGAINST ALUMINIUM SULPHATE INDUCED IN LIVER TOXICITY

2018 ◽  
Vol 10 (2) ◽  
pp. 136
Author(s):  
Usharani S. ◽  
Anuradha R.
Keyword(s):  
Dna Fragmentation ◽  
Liver Toxicity ◽  
Tca Cycle ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Aluminium Sulphate ◽  
Metabolizing Enzymes ◽  
Membrane Bound ◽  
Medicinal Therapy ◽  
Careful Assessment

Objective: The modulation of membrane-bound ATPases, carbohydrate metabolizing enzymes and mitochondrial TCA cycle enzymes in Wattakaka volubilis on aluminium sulphate induced liver toxicity.Methods: Medicinal therapy requires careful assessment of effective treatment offering an acceptable safety over human health. Experimental animals were divided into five groups (Untreated, negative and positive control, hepatic group and the hepatic group fed on Wattakaka volubilis), Metabolising enzymes level, estimation of DNA, RNA and quantification of DNA fragmentation and gene expression were investigated. These altered enzyme levels were ameliorated significantly by administration of Wattakaka volubilis at the concentration of 200 mg/kg in drug-treated animals.Results: Results showed that treatment with methanol extract of Wattakaka volubilis normal level of enzymes which are compared with silymarin. This was evident from the significant increase in p<0.05, p<0.01, p<0.001 enzyme levels. Aluminium sulphate induced rats showed decreased the activities of metabolising enzymes and increased DNA fragmentation in the liver. This clearly explained the reason for the hepatoprotective activity of Wattakaka volubilis leaf extract.Conclusion: The methanolic leaf extract of W. volubilis showed high protective activity against aluminium sulphate-induced hepatotoxicity.

scholarly journals IN-VITRO ANTIOXIDANT AND IN-VIVO HEPATO PROTECTIVE ACTIVITY OF ETHANOL EXTRACTS FROM THE BARK OF SHOREA ROBUSTA (DIPTEROCARPACEAE) AGAINST CARBON TETRA CHLORIDE INDUCED LIVER TOXICITY IN RATS

2016 ◽  
Vol 9 (6) ◽  
pp. 225
Author(s):  
Diptanu Biswas
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Protective Effects ◽  
Shorea Robusta ◽  
Carbon Tetra ◽  
Anti Oxidant ◽  
Ethanol Extracts

ABSTRACT: The study is designed for the evaluation of in-vivo Hepato protective and in-vitro Anti oxidant activity of ethanol extracts from the bark of Shorea robusta (Dipterocarpaceae) by CCl4 induced hepatotoxicity in rats. Ethanol extracts from the bark Shorea robusta (EESR) was evaluated for hepatoprotective activity in rats by inducing liver damage by CCl4. The anti oxidant activity of EESR was assayed by various in-vitro antioxidant methods and activities were compared to standard ascorbic acid. Ethanol extracts at an oral dose 200mg/kg and 400mg/kg exhibited a significant (*p<0.005) protective effects by lowering the level of SGOT, SGPT, ALP, Serum bilirubin, total cholesterol and increasing the level of total proteins as compared to Silymarin (50mg/kg) used as positive control. The extracts exhibit significant anti oxidant activity in various in vitro anti oxidant models.  From these studies we are concluding that, the ethanolic extracts of S.robusta have potent hepatoprotective effects and have anti oxidant properties, hence can be used as a natural product against liver damage.KEY WORDS: Anti oxidant, Carbon tetra chloride,  Hepatoprotective,  Shorea robusta

scholarly journals AMELIORATION OF ACETAMINOPHEN-INDUCED HEPATOTOXICITY USING SOLANUM TORVUM SW. FRUIT EXTRACT IN WISTAR RATS

2019 ◽  
pp. 238-242
Author(s):  
UMERA BEGAM AK ◽  
SENTHILKUMAR R ◽  
SIRAJUDEEN J
Keyword(s):  
Aqueous Extract ◽  
Tca Cycle ◽  
Hepatoprotective Activity ◽  
Marker Enzymes ◽  
Fruit Extract ◽  
Solanum Torvum ◽  
Medicinal Uses ◽  
Membrane Bound ◽  
Histopathological Studies

Objective: Ayurveda and Chinese pharmacopeia have highlighted the traditional medicinal uses of Solanum torvum Sw. The fruits are ethnomedical used in the treatment of liver and spleen enlargement, cough, and also used as a hematopoietic, antimicrobial, and analgesic agent. In the present study, the amelioration of acetaminophen (APAP)-induced hepatotoxicity of the aqueous extract of S. torvum Sw. fruits is evaluated. Methods: The hepatoprotective activity of the fruit extract against APAP insult was evaluated by assessing it is in vivo antioxidants status, membrane-bound adenosine triphosphatases (ATPases), and tricarboxylic acid (TCA) cycle marker enzymes and also through histopathological studies of the liver. Results: Administration of the aqueous fruit extract of the plant caused a significant increase in the in vivo antioxidant status as evident from the reduction in lipid peroxidation caused by APAP and improvement in the mitochondrial membrane stability which is proved from the activity of membrane-bound ATPases and TCA cycle marker enzymes. Histological studies also supported the fact that the plant extract proved to revive the architecture of the toxin damaged liver tissues in par with silymarin. The chemical pathological changes were consistent with histopathological observations suggesting marked hepatoprotective effect of the aqueous extract of S. torvum. Conclusion: The results showed that the extract of S. torvum Sw. fruits has hepatoprotective potential which may be due to the antioxidant activity of its phytoconstituents, especially flavonoids, alkaloids, phenolics, etc.

scholarly journals Hepatoprotective Activity of Methanolic Extract ofBauhinia purpureaLeaves against Paracetamol-Induced Hepatic Damage in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
F. Yahya ◽  
S. S. Mamat ◽  
M. F. F. Kamarolzaman ◽  
A. A. Seyedan ◽  
K. F. Jakius ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Liver Toxicity ◽  
Enzyme Level ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Total Phenolic ◽  
Bauhinia Purpurea

In an attempt to further establish the pharmacological properties ofBauhinia purpurea(Fabaceae), hepatoprotective potential of methanol extract ofB. purpurealeaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n=6) were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease (P<0.05) in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation.

Kaempferol ameliorates aflatoxin B1 (AFB1) induced hepatocellular carcinoma through modifying metabolizing enzymes, membrane bound ATPases and mitochondrial TCA cycle enzymes

2012 ◽  
Vol 2 (3) ◽  
pp. S1653-S1659 ◽  
Author(s):  
Kulanthaivel Langeswaran ◽  
Rajendran Revathy ◽  
Subbaraj Gowtham Kumar ◽  
Shanmugam Vijayaprakash ◽  
Maruthaiveeran Periyasamy Balasubramanian
Keyword(s):  
Hepatocellular Carcinoma ◽  
Aflatoxin B1 ◽  
Tca Cycle ◽  
Metabolizing Enzymes ◽  
Tca Cycle Enzymes ◽  
Membrane Bound

Hepatoprotective activity of the wild carrot chloroform-based fraction against CCl4-induced liver toxicity in mice

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
W Shebaby ◽  
M El-Sibai ◽  
M Mroueh ◽  
K Bodman-Smith ◽  
R Taleb ◽  
...  
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Wild Carrot

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

scholarly journals Hepatoprotective Activity and Nephroprotective Activity of Peel Extract from Three Varieties of the Passion Fruit (Passiflora Sp.) in the Albino Rat

2019 ◽  
Vol 7 (4) ◽  
pp. 536-542 ◽  
Author(s):  
Nerdy Nerdy ◽  
Kiking Ritarwan
Keyword(s):  
Body Weight ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Passion Fruit ◽  
Fruit Peel ◽  
Albino Rat ◽  
Nephroprotective Activity ◽  
Purple Passion Fruit ◽  
Peel Extract ◽  
Yellow Passion Fruit

BACKGROUND: The Passion Fruit (Passiflora sp.) that grows in the Indonesian region generally has three varieties, namely purple passion fruit (Passiflora edulis Sims.), red passion fruit (Passiflora ligularis Juss.), and yellow passion fruit (Passiflora verrucifera Lindl.). The passion fruit peel is an economic waste that has not been utilised optimally, but has many efficacious phytochemical contents. AIM: The objectives of this research are to examine scientifically hepatoprotective activity (with paracetamol-induced hepatotoxic) and nephroprotective activity (with gentamicin-induced nephrotoxic) from three varieties of the passion fruit (purple passion fruit peel extract, red passion fruit peel extract and yellow passion fruit peel extract) in the albino rat (Rattus norvegicus). METHODS: Three varieties of passion fruit peels were extracted by maceration method. The experimental animals used were the albino rat (Rattus norvegicus). Hepatoprotective activity was done by the liver biochemical (alanine transaminase and aspartate transaminase) analysis with paracetamol (hepatotoxic compound) induced after 10 days of treatment with extract. Nephroprotective activity was done by the kidney biochemical (urea and creatinine) analysis with gentamicin (nephrotoxic compound) induced after 10 days of treatment with extract. RESULTS: The hepatoprotective activity for positive control was similar to the 250 mg of purple passion fruit peel extract per kg of body weight, 250 mg of red passion fruit peel extract per kg of body weight, and 500 mg of yellow passion fruit peel extract per kg of body weight. The nephroprotective activity for positive control (50 mg of silymarin per kg of body weight) was similar to the 250 mg of purple passion fruit peel extract per kg of body weight, 500 mg of red passion fruit peel extract per kg of body weight, and 500 mg of yellow passion fruit peel extract per kg of body weight. CONCLUSIONS: The extracts were shown hepatoprotective activity and nephroprotective activity with a dose-dependent activity. The hepatoprotective activity and nephroprotective activity of purple passion fruit peel extract were the best compared to red passion fruit peel extract and yellow passion fruit peel extract.

Antioxidant and Hepatoprotective Effects of Orthosiphon stamineus Benth.

2007 ◽  
Vol 35 (01) ◽  
pp. 115-126 ◽  
Author(s):  
Mun Fei Yam ◽  
Rusliza Basir ◽  
Mohd. Zaini Asmawi ◽  
Zhari Ismail
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Liver Function Tests ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Orthosiphon Stamineus

Orthosiphon stamineus (OS), Benth. (Lamiaceae) is widely used in Malaysia for treatments of various kidney and liver ailments. In the experiment, DPPH• radicals scavenging, Fe 3+-induced lipid peroxidation inhibiting activities and trolox equivalent antioxidant capacity (TEAC) of methanol/water extract of Orthosiphon stamineus (SEOS) were determined. The results indicated that SEOS exhibited antioxidant, lipid peroxidation inhibition and free radical scavenging activities. The hepatoprotective activity of the SEOS was studied using CCl 4-induced liver toxicity in rats. The activity was assessed by monitoring liver function tests through the measurement of alanine transaminase (ALT) and aspartate transaminase (AST). Furthermore, hepatic tissues were also subjected to histopathological studies. Pretreatment of SEOS (125, 250, 500 and 1000 mg/kg p.o.) dose-dependently reduced the necrotic changes in rat liver and inhibited the increase of serum ALT and AST activities. The results of the present study indicated that the hepatoprotective effect of Orthosiphon stamineus might be ascribable to its antioxidant and free radical scavenging property.

108 NEGLIGIBLE LEVEL OF DNA DAMAGE IN BOVINE OOCYTES VITRIFIED USING MINIMUM VOLUME METHODS

2006 ◽  
Vol 18 (2) ◽  
pp. 162
Author(s):  
K. Papis ◽  
E. Stachowiak ◽  
M. Kruszewski ◽  
T. Iwanenko ◽  
T. Bartlomiejczyk
Keyword(s):  
Comet Assay ◽  
Dna Fragmentation ◽  
Sucrose Solution ◽  
Objective Assessment ◽  
Significant Proportion ◽  
Positive Control ◽  
Negative Control ◽  
Dna Integrity ◽  
Minimum Volume ◽  
Bovine Oocytes

A relatively high number of bovine cryopreserved oocytes analyzed by the comet assay (Men et al. 2003 Mol. Reprod. Dev. 64, 245) showed compromised DNA integrity. The DNA fragmentation (comet tails) was found in 29% of slow cooled oocytes, in 20% of oocytes vitrified in straws and in 24% of oocytes vitrified in open pulled straws (OPS). Present study used the comet assay to compare the DNA status of 151 in vitro matured bovine oocytes vitrified in straws, in OPS or in droplets. It was assumed that the droplet method (Papis et al. 2000 Theriogenology 54, 651), which has gentle pre-equilibration prior to vitrification, would offer better protection of DNA. OPS vitrification was performed using a solution consisting of 20% DMSO, 20% ethylene glycol (EG), and 0.5 M sucrose. For in-straw and in-droplet vitrification, VS14 (5.5 M EG and 1.0 M sucrose) solution was used. In these two methods pre-equilibration in 3% EG solution for 15 min was applied. Fresh oocytes exposed to 0.5 mM of hydrogen peroxide for 5 min served as the positive control. Fresh M II oocytes served as the negative control. The comet assay was performed according to the procedure of Men et al. (2003) with some modifications aimed at enhancing the sensitivity of the method. The zona pellucida was removed using 0.5% pronase solution, followed by placing of the oocytes in droplets of low-melting agarose on slides. These were subjected to overnight treatment in lysis buffer, followed by 40 min of DNA unfolding and 30 min electrophoresis. Following air drying, the slides were stained with DAPI fluorochrome and photographed. The pictures were saved as anonymous consecutive files to enable objective assessment. Of 119 vitrified oocytes, 112 (94%) were evaluated. The remainder were lost or displayed atypical pictures. The comets could not be analyzed with the Comet v.3.0 software, possibly due to the large size of each oocyte. Six main classes of comet tails were distinguished ranging from no tail (class 0) to heavy and long tail (class 5). Positive control oocytes displayed class 4 (36%) or 5 (64%) tails. Negative control oocytes formed class 0 (18%) to class 3 (47%) comet tails. The oocytes vitrified using minimum volume methods fell within the same range, with 80% and 76% of oocytes vitrified in droplets and OPS, respectively, forming class 1 or 2 tails. One OPS vitrified oocyte (2.2%) expressed a class 5 tail. A small but significant proportion of oocytes vitrified in straws (15.4%, P d 0.05, ANOVA) formed class 4 tails typical of positive control oocytes. In conclusion, in spite of pre-equilibration, a significant proportion of oocytes vitrified in straws contained detectable levels of DNA fragmentation, due probably to the lower cooling rate. The minimum volume protocols (the droplet and OPS methods) caused virtually no damage as assessed by the DNA comet assay. Results presented here differ from those reported previously. Reasons for differences remain to be established.

Sign in / Sign up

Export Citation Format

Share Document