Hepatoprotective activity of the wild carrot chloroform-based fraction against CCl4-induced liver toxicity in mice

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
W Shebaby ◽  
M El-Sibai ◽  
M Mroueh ◽  
K Bodman-Smith ◽  
R Taleb ◽  
...  
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Wild Carrot

scholarly journals IN-VITRO ANTIOXIDANT AND IN-VIVO HEPATO PROTECTIVE ACTIVITY OF ETHANOL EXTRACTS FROM THE BARK OF SHOREA ROBUSTA (DIPTEROCARPACEAE) AGAINST CARBON TETRA CHLORIDE INDUCED LIVER TOXICITY IN RATS

2016 ◽  
Vol 9 (6) ◽  
pp. 225
Author(s):  
Diptanu Biswas
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Protective Effects ◽  
Shorea Robusta ◽  
Carbon Tetra ◽  
Anti Oxidant ◽  
Ethanol Extracts

ABSTRACT: The study is designed for the evaluation of in-vivo Hepato protective and in-vitro Anti oxidant activity of ethanol extracts from the bark of Shorea robusta (Dipterocarpaceae) by CCl4 induced hepatotoxicity in rats. Ethanol extracts from the bark Shorea robusta (EESR) was evaluated for hepatoprotective activity in rats by inducing liver damage by CCl4. The anti oxidant activity of EESR was assayed by various in-vitro antioxidant methods and activities were compared to standard ascorbic acid. Ethanol extracts at an oral dose 200mg/kg and 400mg/kg exhibited a significant (*p<0.005) protective effects by lowering the level of SGOT, SGPT, ALP, Serum bilirubin, total cholesterol and increasing the level of total proteins as compared to Silymarin (50mg/kg) used as positive control. The extracts exhibit significant anti oxidant activity in various in vitro anti oxidant models.  From these studies we are concluding that, the ethanolic extracts of S.robusta have potent hepatoprotective effects and have anti oxidant properties, hence can be used as a natural product against liver damage.KEY WORDS: Anti oxidant, Carbon tetra chloride,  Hepatoprotective,  Shorea robusta

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

Antioxidant and Hepatoprotective Effects of Orthosiphon stamineus Benth.

2007 ◽  
Vol 35 (01) ◽  
pp. 115-126 ◽  
Author(s):  
Mun Fei Yam ◽  
Rusliza Basir ◽  
Mohd. Zaini Asmawi ◽  
Zhari Ismail
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Liver Function Tests ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Orthosiphon Stamineus

Orthosiphon stamineus (OS), Benth. (Lamiaceae) is widely used in Malaysia for treatments of various kidney and liver ailments. In the experiment, DPPH• radicals scavenging, Fe 3+-induced lipid peroxidation inhibiting activities and trolox equivalent antioxidant capacity (TEAC) of methanol/water extract of Orthosiphon stamineus (SEOS) were determined. The results indicated that SEOS exhibited antioxidant, lipid peroxidation inhibition and free radical scavenging activities. The hepatoprotective activity of the SEOS was studied using CCl 4-induced liver toxicity in rats. The activity was assessed by monitoring liver function tests through the measurement of alanine transaminase (ALT) and aspartate transaminase (AST). Furthermore, hepatic tissues were also subjected to histopathological studies. Pretreatment of SEOS (125, 250, 500 and 1000 mg/kg p.o.) dose-dependently reduced the necrotic changes in rat liver and inhibited the increase of serum ALT and AST activities. The results of the present study indicated that the hepatoprotective effect of Orthosiphon stamineus might be ascribable to its antioxidant and free radical scavenging property.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF CORDIA OBLIQUA AGAINST PARACETAMOL-INDUCED LIVER TOXICITY ON WISTAR RATS

2019 ◽  
pp. 149-152
Author(s):  
SIVAKRISHNAN SIVAGNANAM ◽  
PRADEEPRAJ DEVARASU
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Histological Study ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Plant Materials ◽  
Percolation Method ◽  
Soxhlet Apparatus ◽  
Serum Glutamate Pyruvate Transaminase

Objective: The ethanolic extract of Cordia obliqua (EECO) Willd. leaves was evaluated for hepatoprotective activity in Wistar rats by inducing hepatic damage with paracetamol. Methods: The leaves of C. obliqua were dried under shade, segregated, pulverized by a mechanical grinder and passed through a 40 mesh sieve. The powdered plant materials were stored in an air-tight container. The above-powdered materials were successively extracted with ethanol by hot continuous percolation method in Soxhlet apparatus for 24 h. The extract was concentrated using a rotary evaporator and subjected to freeze drying in a lyophilizer till dry powder was obtained. Results: Treatment with EECO at 200 and 400 mg/kg showed significant decrease in serum glutamate-pyruvate transaminase, serum glutamic oxaloacetic transaminase, alkaline phosphatase, and total bilirubin levels and a significant elevation in the total protein, albumin, and globulin levels in serum when compared with paracetamol treated rats. In histological study of liver, the hepatocytes show moderate cytoplasm and moderately enlarged pleomorphic and hyperchromatic nuclei. The portal triads show mild periportal inflammation composed of lymphocytes, and central veins are normal. Conclusion: On the basis of results, we can conclude that the EECO leaves showed potential hepatoprotective activity in rats.

scholarly journals Liquiritigenin Derivatives and Their Hepatotoprotective Activity

2010 ◽  
Vol 5 (8) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Rashmi Gaur ◽  
Sunil Kumar ◽  
Priyanka Trivedi ◽  
Rajendra Singh Bhakuni ◽  
Dnyaneshwar Umrao Bawankule ◽  
...  
Keyword(s):  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Glycyrrhiza Glabra ◽  
Treatment Groups ◽  
Toxicity Biomarkers ◽  
Toxicity In Vitro

Liquiritigenin (7,4’-dihydroxyflavanone), isolated from the roots of Glycyrrhiza Glabra, was derivatized to liquiritigenin 7, 4’-diacetate, liquiritigenin 4’-acetate, isoliquiritigenin, and liquiritigenin 7, 4’-dibenzoate. All these derivatives were evaluated for in vitro hepatoprotective activity against D-galactosamine–lipopolysaccharide(GalN/LPS) induced toxicity. In-vitro hepatotoxicity was manifested by a significant increase ( P < 0.05) in liver toxicity biomarkers (SGPT, SGOT, ALKP, triglyceride, LPO, NO and LDH). The level of biomarkers in the treatment groups was significantly decreased ( P< 0.05) when compared with the GalN/LPS group. The results revealed that isoliquiritigenin exhibited better hepatoprotective activity than liquiritigenin and its derivatives.

scholarly journals Hepatoprotective Activity of BV-7310, a Proprietary Herbal Formulation of Phyllanthus niruri, Tephrosia purpurea, Boerhavia diffusa, and Andrographis paniculata, in Alcohol-Induced HepG2 Cells and Alcohol plus a Haloalkane, CCl4, Induced Liver Damage in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Debendranath Dey ◽  
Sunetra Chaskar ◽  
Narendra Bhatt ◽  
Deepa Chitre
Keyword(s):  
Body Weight ◽  
Liver Damage ◽  
Hepg2 Cells ◽  
Liver Toxicity ◽  
Hepatoprotective Activity ◽  
Andrographis Paniculata ◽  
Phyllanthus Niruri ◽  
In Vivo Models ◽  
Tephrosia Purpurea ◽  
Boerhavia Diffusa

Excessive alcohol consumption is a worldwide threat with severe morbidity and mortality. Other than abstinence, there is still no FDA-approved drug for alcoholic liver disease (ALD). Liver is the primary site of ethanol metabolism and hence gets the most damage from excessive drinking. It triggers multiple signalling events including inflammation, leading to an array of hepatic lesions like steatosis, hepatitis, fibrosis, and cirrhosis. Similarly, when medications or xenobiotic compounds are ingested orally, the liver gets the highest exposure of those metabolites, which in turn can cause severe liver toxicity. BV-7310 is a standardized mixture of four Ayurvedic plants, namely, Phyllanthus niruri, Tephrosia purpurea, Boerhavia diffusa, and Andrographis paniculata. In different systems of traditional medicine, each of these plants has been known to have use in gastrointestinal disorders. We wanted to assess the combined effect of these plant extracts on alcohol-induced liver damage. First, we investigated the hepatoprotective activity of BV-7310 against alcohol-induced toxicity in human liver HepG2 cells. Ethanol treatment (120 mM for 48 hours) significantly showed toxicity (around 42%) in these cells, and coincubation with BV-7310 prevented ethanol-induced cell death in a dose-dependent manner. Interestingly, the formulation BV-7310 showed synergistic activity than any individual extract tested in this assay. BV-7310 also showed potent antioxidant activity in 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. Next, we induced hepatitis in Sprague–Dawley (SD) rats using repeated alcohol (40%) dosing, and carbon tetrachloride (CCl4) 24 hours before termination. Both oral doses of BV-7310 (250 and 500 mg/kg body weight) protected the alcohol-induced body weight loss and significantly improved the elevated levels of liver enzymes compared to the vehicle treated group. Thus, BV-7310 prevents alcohol-induced toxicity in both in-vitro and in-vivo models and could be beneficial for the treatment of ALD or other conditions, which may cause liver toxicity.

scholarly journals Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats

2020 ◽  
Vol 71 (1) ◽  
pp. 345
Author(s):  
S. M. Galal ◽  
M. K.S. Morsi ◽  
M. K. Abd El-Rahman ◽  
S. K. Darwish ◽  
M. A. Katry
Keyword(s):  
Liver Injury ◽  
Unsaponifiable Matter ◽  
Liver Toxicity ◽  
Serum Alkaline Phosphatase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Subcutaneous Administration ◽  
Hepatoprotective Activity ◽  
Serum Marker ◽  
Hepatoprotective Effect

In the present study, the hepatoprotective activity of the unsaponifiable matter (UNSAP) of olive oil, linseed, and sesame oils against CCl4-induced liver toxicity in rats was investigated. In a preliminary antioxidant study, UNSAP showed pronounced DPPH radical scavenging activity (IC50 6.2-10.8 mg/mL). The constituents of UNSAP were determined by GC-MS. The subcutaneous administration of CCl4, caused liver injury. The hepatoprotective effect of UNSAP was comparable to that of α-tocopherol, a standard antioxidant agent. The co-administration of the investigated UNSAP normalized the activities of serum marker enzymes, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Furthermore, the serum alkaline phosphatase (ALP) activity and hepatic malondialdehyde (MDA) level were found to be alleviated by pre-treatment with the UNSAP. A histopathological evaluation showed marked improvement in the liver of UNSAP- and α-Tocopherol-treated animals. The hepatoprotective effect could be attributed to the antioxidant characteristics of UNSAP.

scholarly journals Hepatoprotective Screening of Seriphidium kurramense (Qazilb.) Y.R. Ling

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Maroof Ali ◽  
Hidayat Hussain ◽  
Amjad Hussain ◽  
Abdur Rauf ◽  
Wahid Hussain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Hepatoprotective Activity ◽  
Nitrate Content ◽  
Serum Samples ◽  
Morphological Alterations ◽  
Ccl4 Administration ◽  
Altered Liver

Investigation on medicinal plants’ therapeutic potential has gained substantial importance in the discovery of novel effective and safe therapeutic agents. The present study is aimed at investigating the hepatoprotective potential of Seriphidium kurramense methanolic extract (SKM) against carbon tetrachloride- (CCl4-) induced hepatotoxicity in rats. S. kurramense is one of the most imperative plants for its various pharmacological activities. Therefore, this study was aimed at evaluating the hepatoprotective potential against CCl4-induced liver toxicity. The serum samples were analyzed for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) together with the oxidative stress mediator levels as nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), reduced glutathione (GSH), and superoxide dismutase (SOD) as well as peroxidation and H2O2 activity. CCl4 administration resulted in an elevated free radical generation, altered liver marker (AST and ALT) enzymes, reduced antioxidant enzyme, and increased DNA damage. Methanolic extract of S. kurramense decreased CCl4-induced hepatotoxicity by increasing the antioxidant status and reducing H2O2 and nitrate content generation as well as reducing DNA damage. Additionally, SKM reversed the morphological alterations induced by CCl4 in the SKM-treated groups. These results demonstrated that SKM displayed hepatoprotective activity against CCl4-induced hepatic damage in experimental rats.

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