Carvacrol activity & morphological changes in Mycobacterium tuberculosis

2018 ◽  
Vol 13 (8) ◽  
pp. 877-888 ◽  
Author(s):  
Sandra S Nakamura de Vasconcelos ◽  
Katiany R Caleffi-Ferracioli ◽  
Laíse A Hegeto ◽  
Vanessa P Baldin ◽  
Celso V Nakamura ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Morphological Changes

scholarly journals Morphological changes and differentially expressed efflux pump genes in Mycobacterium tuberculosis exposed to a rifampicin and verapamil combination

Tuberculosis ◽  
2016 ◽  
Vol 97 ◽  
pp. 65-72 ◽  
Author(s):  
Katiany R. Caleffi-Ferracioli ◽  
Renata Claro R. Amaral ◽  
Fernanda O. Demitto ◽  
Flaviane G. Maltempe ◽  
Pedro Henrique Canezin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Efflux Pump ◽  
Morphological Changes ◽  
Differentially Expressed

Therapeutic Effects of Citric and Succinic Acids in Rats Exposed to Inactivated M. tuberculosis

2021 ◽  
pp. 69-75
Author(s):  
SV Skupnevskiy ◽  
GM Trukhina ◽  
EG Pukhaeva ◽  
AK Badtiev ◽  
FK Rurua ◽  
...  
Keyword(s):  
Body Weight ◽  
Mycobacterium Tuberculosis ◽  
Succinate Dehydrogenase ◽  
Blood Cells ◽  
Morphological Changes ◽  
White Blood Cells ◽  
Therapeutic Effects ◽  
X Rays ◽  
Freund’S Adjuvant ◽  
Freund's Adjuvant

Introduction. The search for methods of correcting pathogenetic disorders related to Mycobacterium tuberculosis, the causative agent of tuberculosis, a highly hazardous communicable and socially significant disease, determines the relevance of the research and its objective to study the role of citric and succinic acids in protective and adaptive processes in warm-blooded animals with connective tissue disorders induced by inactivated mycobacteria. Materials and methods. The study was conducted on male Wistar rats with diseases induced by complete Freund’s adjuvant (a mineral oil emulsion containing heat-killed Mycobacterium tuberculosis). The animals were given a feed-added mixture of organic acids at 17 mg/kg body weight (minimum) and 88 mg/kg body weight (maximum) for 4 weeks. Hematology and bio�chemistry tests were performed using standard methods. The activity of succinate dehydrogenase in blood lymphocytes was determined by the cytobiochemical method. X-rays were obtained using stationary veterinary imaging equipment. Results. The protective effect of carboxylic acids in the exposed animals with Freund’s adjuvant-induced leukocytosis (expressed by a 28 % increase in white blood cells compared to the negative control, p < 0.05), oxidative stress (expressed by an increase in the concentration of malondialdehyde (MDA) by 40 %, p < 0.001, and in inhibition of catalase by 4 %), and subchondral bone sclerosis was characterized by a dose-dependent reduction in immunotoxic manifestations of the disease such as normalization of the number of white blood cells (p < 0.05 compared to model animals); a 27 % reduction in MDA, p < 0.001, a 10 % catalase activation, p < 0.01; succinate dehydrogenase normalization, and a decrease in dystrophic changes in the articular system of animals. Conclusion. The results of hematological, biochemical and radiological tests prove that pathological biochemical and morphological changes related to administration of inactivated M. tuberculosis to warm-blooded animals can be modified by a mixture of citric and succinic acids added to feed, which allows a better understanding of the pathogenesis and an increased therapy effectiveness.

scholarly journals Human Eosinophil Peroxidase Induces Surface Alteration, Killing, and Lysis of Mycobacterium tuberculosis

2003 ◽  
Vol 71 (2) ◽  
pp. 605-613 ◽  
Author(s):  
Violetta Borelli ◽  
Francesca Vita ◽  
Sandeep Shankar ◽  
Maria Rosa Soranzo ◽  
Elena Banfi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Mycobacterium Tuberculosis ◽  
Morphological Changes ◽  
Time Dependent ◽  
Morphological Evidence ◽  
Human Eosinophil ◽  
Molar Basis ◽  
Eosinophil Peroxidase ◽  
Surface Alteration

ABSTRACT The antimycobacterial role of eosinophil peroxidase (EPO), one of the most abundant granule proteins in human eosinophils, was investigated. Our data indicate that purified EPO shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv. On a molar basis, this activity was similar to that exhibited by neutrophil myeloperoxidase (MPO) and was both dose and time dependent. In contrast to the activity of MPO, which requires H2O2, EPO also exhibited anti-M. tuberculosis activity in the absence of exogenously added peroxide. Morphological evidence confirmed that the mechanism of action of EPO against mycobacteria differs from that of MPO. While MPO kills M. tuberculosis H37Rv exclusively in the presence of hydrogen peroxide, it does not induce morphological changes in the pathogen. In contrast, EPO-treated bacteria frequently had cell wall lesions and eventually underwent lysis, either in the presence or in the absence of H2O2.

scholarly journals Morphological changes of the small intestine mucosa in HIV/Mycobacterium tuberculosis coinfection

2021 ◽  
Vol 56 (2) ◽  
pp. 193-200
Author(s):  
Liliia D. TODORIKO ◽  
◽  
Olena V. PIDVERBETSKA ◽  
Olga S. SHEVCHENKO ◽  
Ihor O. SEMIANIV ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Small Intestine ◽  
Morphological Changes ◽  
Intestine Mucosa

scholarly journals Impact of LytR-CpsA-Psr Proteins on Cell Wall Biosynthesis in Corynebacterium glutamicum

2016 ◽  
Vol 198 (22) ◽  
pp. 3045-3059 ◽  
Author(s):  
Meike Baumgart ◽  
Karin Schubert ◽  
Marc Bramkamp ◽  
Julia Frunzke
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Corynebacterium Glutamicum ◽  
Morphological Changes ◽  
Cell Wall Biosynthesis ◽  
Special Focus ◽  
Content Type ◽  
Cell Wall Biogenesis ◽  
Exact Function ◽  
The Impact

ABSTRACT Proteins of the LCP (LytR, CpsA, Psr) family have been shown to inherit important roles in bacterial cell wall biosynthesis. However, their exact function in the formation of the complex cell wall structures of the Corynebacteriales , including the prominent pathogens Mycobacterium tuberculosis and Corynebacterium diphtheriae , remains unclear. Here, we analyzed the role of the LCP proteins LcpA and LcpB of Corynebacterium glutamicum , both of which localize at regions of nascent cell wall biosynthesis. A strain lacking lcpB did not show any growth-related or morphological phenotype under the tested conditions. In contrast, conditional silencing of the essential lcpA gene resulted in severe growth defects and drastic morphological changes. Compared to the wild-type cell wall, the cell wall of this mutant contained significantly less mycolic acids and a reduced amount of arabinogalactan. In particular, rhamnose, a specific sugar component of the linker that connects arabinogalactan and peptidoglycan, was decreased. Complementation studies of the lcpA -silencing strain with several mutated and truncated LcpA variants suggested that both periplasmic domains are essential for function whereas the cytoplasmic N-terminal part is dispensable. Successful complementation experiments with proteins of M. tuberculosis and C. diphtheriae revealed a conserved function of LCP proteins in these species. Finally, pyrophosphatase activity of LcpA was shown in an in vitro assay. Taken together, our results suggest that LCP proteins are responsible for the transfer of arabinogalactan onto peptidoglycan in actinobacterial species and support a crucial function of a so-far-uncharacterized C-terminal domain (LytR_C domain) which is frequently found at the C terminus of the LCP domain in this prokaryotic phylum. IMPORTANCE About one-third of the world's population is infected with Mycobacterium tuberculosis , and multiple-antibiotic resistance provokes the demand for novel antibiotics. The special cell wall architecture of Corynebacteriales is critical for treatments because it is either a direct target or a barrier that the drug has to cross. Here, we present the analysis of LcpA and LcpB of the closely related Corynebacterium glutamicum , the first of which is an essential protein involved in cell wall biogenesis. Our work provides a comprehensive characterization of the impact of LCP proteins on cell wall biogenesis in this medically and biotechnologically important class of bacteria. Special focus is set on the two periplasmic LcpA domains and their contributions to physiological function.

Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

1977 ◽  
Vol 35 ◽  
pp. 484-485
Author(s):  
P. Bagavandoss ◽  
JoAnne S. Richards ◽  
A. Rees Midgley
Keyword(s):  
Cell Surface ◽  
Granulosa Cell ◽  
Morphological Changes ◽  
Follicular Development ◽  
Female Rats ◽  
Functional Changes ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

Effect of verapamil on cytoplasmic distribution of granules and microfilaments in amphibian urinary bladder

1988 ◽  
Vol 46 ◽  
pp. 324-325
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Morphological Changes ◽  
Granular Cell ◽  
Cytosolic Calcium ◽  
Calcium Storage ◽  
Amphibian Urinary Bladder ◽  
Vesicular Membrane ◽  
High Concentrations ◽  
Cytoskeletal System

The amphibian urinary bladder has been used as a ‘model’ system for studies of the mechanism of action of antidiuretic hormone (ADH) in stimulating transepithelial water flow. The increase in water permeability is accompanied by morphological changes that include the stimulation of apical microvilli, mobilization of microtubules and microfilaments and vesicular membrane fusion events . It has been shown that alterations in the cytosolic calcium concentrations can inhibit ADH transmembrane water flow and induce alterations in the epithelial cell cytomorphology, including the cytoskeletal system . Recently, the subapical granules of the granular cell in the amphibian urinary bladder have been shown to contain high concentrations of calcium, and it was suggested that these cytoplasmic constituents may act as calcium storage sites for intracellular calcium homeostasis. The present study utilizes the calcium antagonist, verapamil, to examine the effect of calcium deprivation on the cytomorphological features of epithelial cells from amphibian urinary bladder, with particular emphasis on subapical granule and microfilament distribution.

Hydrated form of some clay minerals observed using a film sealed environmental cell

1978 ◽  
Vol 36 (1) ◽  
pp. 72-73
Author(s):  
N. Kohyama ◽  
K. Fukushima ◽  
A. Fukami
Keyword(s):  
Clay Minerals ◽  
Morphological Changes ◽  
Carbon Film ◽  
Electron Microscopic Observation ◽  
Adsorbed Water ◽  
Electron Microscopic ◽  
Hydrated Form ◽  
Thin Carbon ◽  
Environmental Cell ◽  
Thin Carbon Film

Since the interlayer or adsorbed water of some clay minerals are quite easily dehydrated in dried air, in vacuum, or at moderate temperatures even in the atmosphere, the hydrated forms have not been observed by a conventional electron microscope(TEM). Recently, specific specimen chambers, “environmental cells(E.C.),” have been developed and confirmed to be effective for electron microscopic observation of wet specimen without dehydration. we observed hydrated forms of some clay minerals and their morphological changes by dehydration using a TEM equipped with an E.C..The E.C., equipped with a single hole copper-microgrid sealed by thin carbon-film, attaches to a TEM(JEM 7A) with an accelerating voltage 100KV and both gas pressure (from 760 Torr to vacuum) and relative humidity can be controlled. The samples collected from various localities in Japan were; tubular halloysite (l0Å) from Gumma Prefecture, sperical halloysite (l0Å) from Tochigi Pref., and intermediate halloysite containing both tubular and spherical types from Fukushima Pref..

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