Statistical Optimization of Prodigiosin Production by Plackett-burman Design for Bacteria Isolated from Indian Marine Soil

The current investigation was conducted to maximise the production of the natural anticancer drug from the microbe isolated from the marine soil sample of the Coromandel Coast of the Bay of Bengal region of India. Yellow to red colour pigmented microbes separated by crowd plate method. Bacteria are producing strong colour product subjected to future study. The isolated strains were detected based on biochemical, morphological, and genetic characteristics. Pigment formation was found to be influenced strongly by conditions of the environment. The water-insoluble pigment extracted by acidified methanol and showed maximum absorbance at 535nm. A statistical screening procedure was adopted to select the optimum condition to produce the pigment. The carbon, nitrogen, medium pH, growth condition temperature and revolution of agitation were screened using the response surface methodology statistical model. The near optimum conditions for the production medium were affected by the concentration of peanut, L-proline, percentage inoculum pH and incubation time. When these conditions were employed yield increased as two-fold as the concentration of prodigiosin 789 mg/l.

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Optimization of Inulinase Production from Garlic by Streptomyces sp. in Solid State Fermentation Using Statistical Designs

Biotechnology Research International ◽

10.4061/2011/708043 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

M. Dilipkumar ◽

M. Rajasimman ◽

N. Rajamohan

Keyword(s):

Response Surface Methodology ◽

Solid State ◽

Response Surface ◽

Solid State Fermentation ◽

Soya Bean ◽

Optimum Conditions ◽

Streptomyces Sp ◽

Burman Design ◽

Statistical Designs ◽

Plackett Burman Design

Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using Garlic as substrate by Streptomyces sp. in solid-state fermentation (SSF). From the experiments, 4 nutrients, namely, NH4NO3, MnSO4⋅7H2O, Soya bean cake, and K2HPO4 were found to be most significant nutrient components. Hence, these 4 components are selected. The selected components were optimized using response surface methodology (RSM). The optimum conditions are NH4NO3—6.63 mg/gds, MnSO4⋅7H2O—26.16 mg/gds, Soya bean cake—60.6 mg/gds, and K2HPO4—5.24 mg/gds. Under these conditions, the production of inulinase was found to be 76 U/gds.

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Optimization of inulinase production using copra waste by K. marxianus var. marxianus

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq091211032d ◽

2010 ◽

Vol 16 (4) ◽

pp. 319-327 ◽

Cited By ~ 8

Author(s):

M. Dilipkumar ◽

M. Rajasimman ◽

N. Rajamohan

Keyword(s):

Response Surface Methodology ◽

Carbon Source ◽

Corn Steep Liquor ◽

Positive Influence ◽

Experimental Designs ◽

Statistical Methodology ◽

Optimum Conditions ◽

Burman Design ◽

Steep Liquor ◽

Plackett Burman Design

Kluyveromyces marxianus var marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett-Burman design. Corn steep liquor, (NH4)2SO4, ZnSO4.7H2O, K2HPO4 and urea were selected based on their positive influence on inulinase production. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: corn steep liquor - 0.0560 (g/gds), (NH4)2SO4 - 0.0084 (g/gds), ZnSO4.7H2O - 0.0254 (g/gds), K2HPO4 - 0.0037 (g/gds) and urea - 0.02147 (g/gds). These conditions were validated experimentally which revealed an enhanced inulinase yield of 372 U/gds.

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Statistical screening of medium components by Plackett–Burman design for lactic acid production by Lactobacillus sp. KCP01 using date juice

Bioresource Technology ◽

10.1016/j.biortech.2005.11.017 ◽

2007 ◽

Vol 98 (1) ◽

pp. 98-103 ◽

Cited By ~ 59

Author(s):

K CHAUHAN ◽

U TRIVEDI ◽

K PATEL

Keyword(s):

Lactic Acid ◽

Acid Production ◽

Lactic Acid Production ◽

Medium Components ◽

Burman Design ◽

Date Juice ◽

Statistical Screening ◽

Plackett Burman Design

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Lactose Inducible Fermentation in Escherichia coli for Improved Production of Recombinant Urate Oxidase: Optimization by Statistical Experimental Designs

10.21203/rs.3.rs-93296/v1 ◽

2020 ◽

Author(s):

Hamid shahbaz mohammadi ◽

Abbas Najjari ◽

Eskandar Omidinia

Keyword(s):

Yeast Extract ◽

Urate Oxidase ◽

Optimum Conditions ◽

Iptg Induction ◽

Lactose Induction ◽

Induction Strategy ◽

Burman Design ◽

Plackett Burman Design ◽

Central Composite ◽

Significant Factors

Abstract The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of chemotherapy-induced hyperuricemia. This study deals with the statistical optimization of lactose inducible fermentation for production of soluble recombinant Aspergillus flavus UOX. 10 variables were investigated by Plackett–Burman design (PBD), and the most significant factors were further optimized by central composite design (CCD). PBD results indicated that glycerol, yeast extract, tryptone, and lactose affected UOX activity significantly. The CCD results showed that the maximum enzyme activity (19.34 U/ml) could be achieved under the optimum conditions of glycerol 0.87 g/L, yeast extract 9.11 g/L, tryptone 10.29 g/L, K2HPO4 1.81 g/L, and lactose 12.79 g/L. When the same induction strategy was tested at shake flask, 19.34 U/mL of UOX activity was obtained, which was 12.5 folds higher than IPTG induction protocol. Furthermore, the lower total cost (0.7 vs. 13.5 €) was additionally feature that confirmed the suitability of the lactose induction method. Collectively, our results showed that design of experiment methodology can be applied as a suitable tool for improved production of UOX using lactose as the inducer.

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