scholarly journals Isolation and characterization of probiotics bacteria from curd, pickle and fermented rice and screening of antimicrobial activity

2019 ◽  
Vol 7 (2) ◽  
pp. 23-29
Author(s):  
Prangya Paramita Acharya ◽  
Ajay Kumar Sahu ◽  
Barsha Barsha Kar ◽  
Soumya Ranjan Meher ◽  
Dr.Kirankumar B
Keyword(s):  
Antibiotic Susceptibility ◽  
Food Industry ◽  
Special Kind ◽  
Good Resistance ◽  
Antimicrobial Susceptibility Test ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Molecular Identification Methods ◽  
Inhibition Zones

The live supplement of microorganisms though the dietary product is known as the probiotics. These are some special kind of bacteria they are healthy for the host organisms. These bacteria commonly found in food and dietary product. In our study, probiotics were isolated from fermented rice, curd and pickle .Total isolated strains were studied for their characterization, and also strains were studied antibacterial also their antibiotic susceptibility quality.  The antibiotic resistance of potential strain was studied using Vancomycin, gentamycin, chloromphenicol, ciprofloxacin and cefataximide. PB1, PB3 shows the good resistance activity to the antibiotics. The curd produced by those strains has good quality and quantity of nutrients.  Specially this study is for isolating, identifying, and characterizing the probiotics .Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, This experiments  shows the better  anti-pathogen activity, and acceptable antibiotic susceptibility; it implies we can use these probiotics in different purpose in food industry for modern food synthesis, for antimicrobial susceptibility test we are taking amplicin, karamycin, tetracycline, tripsin by using of the antibiotics concluded that various inhibition zones of given sample of probiotics.

scholarly journals Isolation and characterization of arsenic resistant soil bacteria and their effects on germination of rice under arsenic contamination

2015 ◽  
Vol 2 (2) ◽  
pp. 229-237
Author(s):  
Istiaq Ahmed ◽  
Md Tofazzal Islam ◽  
Md Akhter Hossain Chowdhury ◽  
Md Kamruzzaman
Keyword(s):  
Contaminated Soils ◽  
Sodium Arsenite ◽  
Agar Plate ◽  
Rice Seedling ◽  
Resistant Bacteria ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Resistant Strains

This study was carried out to isolate, screen and characterize arsenic (As) resistant bacteria from As contaminated soils of Dumrakandi and Matlab under Faridpur and Chandpur districts and to evaluate their efficiency in reducing As toxicity against rice seedlings during germination. Thirteen strains were isolated from the soils which showed resistance to different levels of sodium arsenite (viz. 5, 10, 20 and 40 mM) in both agar plate and broth assay using BSMY I media. Among the isolates, BTL0011, BTL0012, BTL0015 and BTL0022 showed highest resistance to 40 mM sodium arsenite. Gram staining and KOH solubility test revealed that five strains were gram positive and rest eight was gram negative. They grew well in the liquid media at pH 5.5 to 8.5. In-vitro rice seedling bioassay with two superior isolates (BTL0011 and BTL0022) revealed that As resistant strains significantly enhanced seed germination of BRRI dhan29 and BRRI dhan47 at 60 ppm As. This study was laid out in CRD with three replications. The performance of BTL 0022 was superior to BTL0011. The overall results suggest that BTL0011 and BTL0022 can be used for bioremediation of As contaminated soils and to increase the germination and seedling growth of rice in As contaminated soils.Res. Agric., Livest. Fish.2(2): 229-237, August 2015

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

Isolation and Characterization of Starch from Seeds ofAraucaria brasiliensis: A Novel Starch for Application in Food Industry

2006 ◽  
Vol 58 (6) ◽  
pp. 283-291 ◽  
Author(s):  
Luis Arturo Bello-Pérez ◽  
Francisco J. García-Suárez ◽  
Guadalupe Méndez-Montealvo ◽  
João Roberto Oliveira do Nascimento ◽  
Franco Maria Lajolo ◽  
...  
Keyword(s):  
Food Industry ◽  
Isolation And Characterization ◽  
Novel Starch

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from corn rhizosfer

2021 ◽  
Vol 911 (1) ◽  
pp. 012063
Author(s):  
Haswania ◽  
H Karim ◽  
A.A. Azis ◽  
N Iriany ◽  
O Jumadi
Keyword(s):  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Methyl Red ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Sucrose Fermentation ◽  
Phosphate Solubilizing ◽  
Sample Dilution

Abstract The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

scholarly journals Evaluation of synergistic medicinal efficacy of four alkaloidic compounds isolated from Iraqi Allium porrum seeds against staphylococcus aureus bacteria

2021 ◽  
Vol 2063 (1) ◽  
pp. 012026
Author(s):  
Abbas D M Al-Maliki ◽  
Ala’a A H Ai-Maliki ◽  
Maha A A Hameed
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Synergistic Action ◽  
Allium Porrum ◽  
Chemical Production ◽  
Isolation And Characterization ◽  
Inhibition Zones ◽  
Gas Chromatography Mass Spectroscopy ◽  
Medicinal Activity

Abstract Medicinal plants are necessary benefit natural sources for chemical production of different therapies from secondary metabolism. Allium porrum is considered as one of sources of foods and drugs at the same time. So the current work focused on isolation and characterization of four alkaloids from seeds of this plant. These compounds are 2-piperidinone, 2-pyrrolidinone-4-methyl, adenosine-2-deoxy–N-methyl –N-(triflouro acetyl ) and maymarsine by using Gas Chromatography – Mass Spectroscopy (GC-MS) technique. The medicinal activity was achieved to investigate and evaluate the synergistic action of the mixture of the four alkaloidic compounds towards Staphylococcus aureus pathogen. The concentrations of alkaloidic mixture were prepared with values equal to 50,100,125,150,200,250,300,375,400 and 500 mg/ml and they revealed inhibition zones with diameters represented by 31,31,32,35,35,37,41,43,43and 45 mm. So the mixture of the four alkaloidic compounds can be used as natural therapy to treat various inflammatory caused by this pathogenic bacteria.

scholarly journals A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Sutami SUTAMI ◽  
Purwanto PURWANTO ◽  
Retno Rosariastuti
Keyword(s):  
Gold Mining ◽  
Rrna Gene ◽  
Salt Tolerant ◽  
Indigenous Bacteria ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Query Coverage ◽  
Physiological Tests ◽  
Indole Production

Abstract. Sutami, Purwanto, Rosariastuti R. 2021. A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia. Biodiversitas 22: 3785-3791. Isolation and characterization of indigenous bacteria from the soil of traditional gold mining contaminated with mercury is the first step in a series of research to explore and utilize indigenous bacteria in Jendi's area. This study was aimed to determine the characteristics and identity of bacterial isolates from soil of traditional gold mining in Jendi Village, Wonogiri contaminated by mercury. The methods used in this study included bacterial isolation, media preparation, phenotypic identification including; morphological and physiological tests and genotyping tests. The results showed that the bacterial isolate A3 grew optimally in media with the addition of 10% NaCl, at a temperature of 27°C, and pH 9. There were negative reactions to the observations of gram staining, acid production from glucose, indole production, catalase and urease, and positive reactions to oxidation. A neighbor-joining phylogenetic tree based on the 16S rRNA gene sequence showed that the A3 strain was closely related to Sphingosinicella microcystinivorans strain Y2T (JCM 13185T) with 100% Query coverage and a maximum identity of 99.56%.

scholarly journals The potential use of probiotic and beneficial bacteria in the Brazilian dairy industry

2018 ◽  
Vol 85 (4) ◽  
pp. 487-496 ◽  
Author(s):  
Monique Colombo ◽  
Svetoslav Dimitrov Todorov ◽  
Monique Eller ◽  
Luís Augusto Nero
Keyword(s):  
Dairy Products ◽  
Food Industry ◽  
Dairy Industry ◽  
Beneficial Bacteria ◽  
Research And Innovation ◽  
Isolation And Characterization ◽  
Potential Source ◽  
Increasing Demand ◽  
Potential Use

Scientific studies demonstrate the importance of intestinal microbiota to human health and how probiotic microorganisms can positively affect health when administered regularly and in adequate amounts. Probiotic bacteria can be part of fermented products and their functional importance is associated mainly with their metabolism. They are thought to benefit individuals to maintain their health and also to strengthen resistance against various types of diseases. The acceptance of probiotic cultures and products by consumers increased when these bacteria were marketed as natural cultures that help in digestion and health. Considering this, the food industry has an increasing demand for new candidates as probiotic cultures, and the dairy industry has a particular interest for fermented milks and other dairy products, since these are the most common food vehicles for probiotic cultures. Therefore, the dairy industries are increasingly seeking to improve their products with these beneficial bacteria. However, the legal peculiarities and excess of control agencies in Brazil makes the registration of these products and the collection of data very complex. Prospective analysis suggests that probiotic foods have the potential to effect a considerable expansion of the dairy industry, allowing the dairy sector to grow and for these products to be increasingly sought by consumers globally. For this, not only actions on research and innovation are necessary, but also official clarifications on the claims for considerations of microbiological security and functionality of these products. This review aims to elucidate important probiotic research regarding the isolation and characterization of beneficial cultures in Brazil, and to demonstrate the relevance of the dairy chain as a potential source of novel cultures for the development of new probiotic products to expand the Brazilian dairy industry.

scholarly journals Isolation and Characterization of Listeria monocytogenes Isolates from Ready-To-Eat Foods in Florida

2006 ◽  
Vol 72 (7) ◽  
pp. 5073-5076 ◽  
Author(s):  
Yuelian Shen ◽  
Yan Liu ◽  
Yifan Zhang ◽  
Jennifer Cripe ◽  
William Conway ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antibiotic Susceptibility ◽  
Food Samples ◽  
Multiple Antibiotic Resistance ◽  
Isolation And Characterization ◽  
Lineage 2 ◽  
Conventional Methods ◽  
Specific Bacteriophage

ABSTRACT Of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for Listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. Seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an L. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods.

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