Development and Validation of TLC of Flavonoid from the Ethanolic Extract of Plant Enhydra fluctuans

Thin layer chromatography is a technique or an analytical tool to separate the bioactive compound from the mixture of components. In current research work special attention was given to develop specific solvent system and to validate the principle of separation of Flavonoid. The ethanolic extract of plant namely Helencha (in Bengali) was selected for such purpose. After several trials, the presence of Flavonoid which was confirmed by qualitative evaluation and was sepahjvvrated successfully under this study and the process was validated under the circumstances of ICH Guideline. The plant not only contained Flavonoid but there were the presence of little quantity of Alkaloid, Saponin and Tannins also. Due to presence of Flavonoid the ethanolic fraction of the plant may be evaluated for Anti-inflammatory and Anthelmintic activity for further research. The plant Helencha is known as Enhydra fluctuans belongs to the family Astereceae. According to folklore claim the plant is useful for nutrition purpose. Not only that the plant is also useful in Dropsy, anasarca and snake bite. As per the literature survey, the plant has Antioxidant and Analgesic activity. Here the total attention was given to separate Flavonoid from the mixture of Components present in the ethanolic fraction of the leaves of the plant. Keywords: Flavonoid, Alkaloid, ICH Guideline, Enhydra fluctuans, Dropsy, Anaasrca.

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ANTI-BIOFILM POTENTIAL OF MENTHOL PURIFIED FROM MENTHA PIPERITA L. (MINT)

Journal of Humanities, Social and Management Sciences (JHSMS) ◽

10.54112/bcsrj.v2020i1.37 ◽

2020 ◽

Vol 2020 (1) ◽

Author(s):

R Ejaz ◽

S Malik ◽

M Ahmad ◽

H ALi ◽

S Choudhry

Keyword(s):

Column Chromatography ◽

Antibacterial Properties ◽

Ethanolic Extract ◽

Bioactive Compound ◽

Solvent System ◽

Mentha Piperita ◽

Phytochemical Analysis ◽

Antibacterial Drug ◽

Biofilm Inhibition ◽

Maximum Separation

Menthol, a bioactive compound of Mentha piperita (mint) with antibacterial properties was purified by column chromatography to determine its anti-biofilm potential. After phytochemical analysis, TLC was carried out using n-hexane: ethyl acetate: methanol: water (2:2:2:1) as the solvent system for ethanolic extract of mint. TLC achieved the maximum separation of mint constituents with Rf value of 0.68. A purified menthol fraction was obtained after silica gel column chromatography using four different eluting solvents. The menthol obtained was then used to perform biofilm inhibition assay to establish its antibacterial potential. Percentage inhibition was highest for bacillus subtilis (79.4%), as opposed to Pseudomonas aeruginosa (33.6%) and the combination of both bacteria (20%). ELISA reader was used to measure absorbance at 450-620nm and 630 nm. Using 450-620nm filter the values for percentage inhibition lies between 48.6-95% for standard and crude menthol samples. Similarly, at 630nm the values of inhibition lie between 23.4-70.6%. This anti-biofilm property of menthol can be utilized in antibacterial drug formulations.

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DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF SWERTIAMARIN IN BULK AND DOSAGE FORM

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017v9i6.22214 ◽

2017 ◽

Vol 9 (6) ◽

pp. 80

Author(s):

H. Padh ◽

S. Parmar ◽

B. Patel

Keyword(s):

High Performance ◽

Forced Degradation ◽

Stability Indicating ◽

Bulk Drug ◽

Development And Validation ◽

Layer Chromatography ◽

Herbal Formulations ◽

Hptlc Method ◽

Ich Guideline

Objective: In the present study a novel stability-indicating high-performance thin-layer chromatography (HPTLC) method for quantitative determination of Swertiamarin (SW) in bulk drug and formulation has been developed and validated as per ICH guideline Q2 (R1) for global acceptance of standardized herbal formulations.Methods: HPTLC method is developed and validated using solvent ethyl acetate: ethanol: chloroform (3:2.5:4.5 v/v/v) (Rf of SW 0.65±0.04) in the absorbance mode at 243 nm. Various forced degradation conditions were used to check degradation of drug.Results: The method showed a good linear relationship (r2 = 0.9990) in the concentration range 200-700 ng per spot. It was found to be linear, accurate, precise and specific.Conclusion: It can be applied for quality control as well as for stability testing of different dosage forms containing swertiamarin. The developed method is validated as per ICH guideline Q2(R1) for global acceptance of standardized herbal formulations.

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Evaluation of Anthelmentic and Antimicrobial Activity of the Extract of the Root of the Plant Jasminum multiflorum(Andr.)

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4.4219 ◽

2020 ◽

Vol 10 (4) ◽

pp. 157-160

Author(s):

Sandip Kumar Pahari ◽

B.K. Gupta ◽

R. Debnath ◽

A. Das

Keyword(s):

Antimicrobial Activity ◽

Petroleum Ether ◽

Petroleum Ether Extract ◽

Anthelmintic Activity ◽

Ethanolic Extract ◽

Qualitative Evaluation ◽

Maximum Activity ◽

Klebsiella Pneumonia ◽

Ether Extract ◽

Zone Of Inhibition

As per qualitative evaluation in different solvents of the root of the plant satisfies the presence of cardiac glycosides along with trace quantities of steroid and saponins. Among them the petroleum ether extract of the root of the plant Jasminum multiflorum was evaluated for anthelmintic activity and the ethanolic extract was evaluated for antimicrobial activity.1,2 Traditionally this species are used in indolent ulcer, pitta and inflammation. Only few CNS activity are reported on ethanolic extract of aerial part of the plant, though the root of the plant is more potent as per folkore claim. The petroleum ether extract was investigated for anthelmentic activity using earthworm (Pheretima posthuma) at different concentration (5mg/ml – 50 mg/ml). As standard albendazole suspension (10mg/ml) and 3% solution of normal saline was used as control. The death and paralysis time were recorded and compared. Extract exhibit significant anthelmentic activity at (100mg/ml) concentration and found effective.1,3,4The ethanolic fraction of the root of the plant was collected and evaporated to dryness under vacuum to avoid the presence of even less quantity of ethanol in the extract. Two Gram (+ve) bacteria namely Bacillius subtilis, Staphylococcus aureus and two Gram (-ve) ve bacteria namely Escherichia coli and Klebsiella pneumonia were selected for the estimation of antimicrobial activity depending on zone of inhibition. It was seen that 100mg/ml concentration of the extract showed maximum activity against Klebsiella pneumonia with a zone of inhibition 0.5mm and for others it ranged from 0.3 to 0.4 mm. 1,4,5 Keywords: Jasminum multiforum; anthelmintic; antimicrobial; zone of inhibition

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Assessment of Anthelmintic Property and Insilico study of phytocompounds in roots of Dechaschistia crotonifolia Wight & Arn.

10.21203/rs.3.rs-1198187/v1 ◽

2022 ◽

Author(s):

Raveesha Peeriga ◽

Keerthi Priyanka Adarapu ◽

Kavya Sri Sanivar ◽

Jyothsna Kanumuri ◽

Rikith Swamy Akunuri ◽

...

Keyword(s):

Anthelmintic Activity ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Tween 80 ◽

Docking Studies ◽

Vital Role ◽

Standard Drug ◽

Death Time ◽

The Family ◽

And Control

Abstract Background:Worm infections in developing countries were reported high. Phytoconstituents have been a vital role for the treatment of many ailments. The current study was aimed assess for anthelmintic activity of different root extracts of Dechaschistia crotonifolia Wight & Arn. belongs to the family Ebanaceae against Pheretima posthuma. Further Insilico study was carried out for phytocompounds present in Dechaschistia. Results: The chloroform, ethylacetate and ethanol extract of Dechaschistia crotonifolia Wight & Arn. were considered for the study of anthelmintic property on earthworms at concentrations 20 mg/ml, 40 mg/ml and 60 mg/ml. During this study, the parameters paralysis time (Pt) and Death Time (Dt) of adult Indian earthworms was observed. As a standard and control Albendazole 10 mg/ml and 2% Tween 80 in distilled water were taken respectively. The study resulted that ethanolic extract was significant when compared with the Albendazole 10 mg/ml. Docking studies revealed the all phytocompounds in Dechaschistia shown binding affinity, however comparatively scopoletin and stigmasterol had shown a good binding affinitiy about -7.7 Kcal/mol and -7.6 Kcal/mol compared to standard drug Albendazole which was shown about -8.7 Kcal/mol. Conclusion: The study revealed that the ethanol extract of Dechaschistia crotonifolia Wight & Arn. at a concentration of 60mg/ml exhibited a stronger anthelmintic property compared to Albendazole 10mg/ml. A dose dependent anthelmintic activity is exerted by all the extracts in an ascending manner Chloroform<Ethyl acetate<Ethanol. These observations were made evidenced by docking studies of phytocompounds in Dechaschistia as the phytocompounds were shown excellent docking score when compared with standard Albendazole.

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Pharmacognostical studies on leaves of Mussaenda frondosa Linn.

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v12i3.4825 ◽

2021 ◽

Vol 12 (3) ◽

pp. 2139-2146

Author(s):

Shanthi S

Keyword(s):

High Performance ◽

Research Work ◽

Ethanol Extract ◽

Correct Identification ◽

Phytochemical Screening ◽

The Family ◽

Rf Values ◽

Phytochemical Studies ◽

Phytochemical Profiles ◽

Layer Chromatography

The Mussaenda frondosa Linn. belonging to the family Rubiaceae, commonly known as Sriparnah in Sanskrit, is a scandent shrub traditionally used in the treatment of cough, bronchitis, fever, inflammation, wounds, ulcers, jaundice, leucoderma and pruritus. Though it is an important plant, till date, no pharmacognostical reports have been available on its leaf. Therefore, the present investigation was undertaken to ascertain the requisite pharmacognostical standards for the standardization of the Mussaenda frondosa leaves. Various investigations like Pharmacognostical studies, preliminary phytochemical screening and High-Performance Thin Layer Chromatography (HPTLC) analysis were carried out, and the salient qualitative parameters were reported. Microscopical evaluation of the leaf revealed the presence of paracytic stomata, microcrystal’s, Idioblast, collenchymas, sand crystals and unicellular unbranched covering Trichomes. The presence of flavonoids, steroids, glycosides, mucilage, saponins and proteins were confirmed through Preliminary phytochemical studies. The HPTLC profile of ethanol extract from M. frondosa L. revealed ten phytoconstituents of Rf value ranging from 0.11 to 0.88. The significant peaks are observed at Rf values of 0.11, 0.16, 0.23 and 0.81. These findings provide referential information for correct identification and standardization of the Musssaenda frondosa leaves, even in powder form. This information will also be useful to distinguish Mussaenda frondosa from the closely related other species of Mussaenda. The Pharmacognostic and phytochemical profiles reported in this research work for Mussaenda frondosa may play a major role in setting monograph of the plant, which might be helpful in proper identification of the plant.

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HPTLC analysis of berberine in stem extracts of Fibraurea darshani

Annals of Plant Sciences ◽

10.21746/aps.2018.7.2.7 ◽

2018 ◽

Vol 7 (2) ◽

pp. 2021

Author(s):

Sheema Dharmapal ◽

Bindu T.K. ◽

Elyas K.K.

Keyword(s):

High Performance ◽

Uv Light ◽

Solvent System ◽

Plant Sample ◽

Phytochemical Analysis ◽

Methanolic Extracts ◽

Uv Reflectance ◽

The Family ◽

Rf Values ◽

Layer Chromatography

The present study is a first report on the phytochemical analysis of the plant Fibraurea darshani which is endemic to Western Ghats. The plant is a woody dioecious climber belonging to the family Menispermaceae. Preliminary phytochemical screening of methanolic extracts of the stem of F. darshani revealed the presence of secondary metabolites like alkaloids, carbohydrates, anthraquiones, terpenoids, flavonoids, phenolics, sterols etc. A simple and reproducible high performance thin layer chromatography was developed to evaluate the presence of berberine in methanol extract of stem of F. darshani. This method involves separation of compounds by HPTLC on pre-coated silica gel 60F 254 plates with a solvent system of Chloroform: Ethyl acetate: Methanol: Formic acid (4:5:4:0.3) and scanned using densitometric scanner in UV reflectance photo mode at 254 and 366nm. The Rf values (0.97) for berberine in the plant sample and the reference standard were found comparable under UV light at 366nm. The HPTLC method developed was simple, accurate and specific.

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ANTIMICROBIAL INVESTIGATION OF AMMANIA BACCIFERA LINN. AGAINST SOME URINARY AND GASTRO-INTESTINAL TRACT INFECTION CAUSING PATHOGENS

INDIAN DRUGS ◽

10.53879/id.49.12.p0044 ◽

2012 ◽

Vol 49 (12) ◽

pp. 44-48

Author(s):

S Sahoo ◽

◽

D Nayak ◽

G.S Kumar ◽

S. Jayakumari

Keyword(s):

High Performance ◽

Ethanolic Extract ◽

Pathogenic Fungi ◽

Salmonella Typhi ◽

Assay Method ◽

Root Extracts ◽

Whole Plant ◽

The Family ◽

Gastro Intestinal Tract ◽

Layer Chromatography

The plant Ammania baccifera Linn., commonly known as Jangli mendi, belonging to the family Lythraceae was investigated for its antimicrobial activity against some selected urinary tract (UT) and gastrointestinal tract (GIT) infection causing pathogens and human pathogenic fungi. The ethanolic extract of the whole plant as well as its leaves, stems and roots were screened to evaluate the antibiogram pattern followed by High Performance Thin Layer Chromatography (HPTLC) study. The extracts were then screened for their antibacterial activity against some UTI and GIT infection causing pathogens viz. Staphylococcus aureus MTCC*1430, Enterococcus faecalis MTCC 2729, Escherichia coli MTCC 118, Pseudomonas aeruginosa MTCC 1035, Klebsiella pneumoniae MTCC 109, Proteus mirabilis MTCC 743, Salmonella typhi, Vibrio cholerae and antifungal activity against some human pathogenic fungi viz. Aspergillus niger MTCC 1344, Candida albicans MTCC 3017, Candida tropicalis, Candida krusei by disc diffusion assay method. The minimum inhibitory concentration (MIC) was evaluated by two fold serial dilution assay method. The HPTLC study of the ethanolic extract of whole plant revealed the presence of eight number of components in the chromatogram developed at 254 nm. The ethanolic extract of. whole plant of A. baccifera showed highest antibacterial efficiency followed by leaf, stem and root extracts respectively. However, ethanolic extracts of whole plant and leaves exhibited moderate antifungal potency followed by stem and root extracts, respectively. The results of MIC indicated that the whole plant extract inhibited P. mirabilis, K. pneumoniae, S. typhi and A. niger at a concentration of 62.5 g/ml thus exhibited broad spectrum of inhibition.

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IN-VITRO ANTIOXIDANT ACTIVITY OF CANNA INDICA EXTRACTS USING DIFFERENT SOLVENT SYSTEM

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2016.v9i6.10583 ◽

2016 ◽

Vol 9 (6) ◽

pp. 53 ◽

Cited By ~ 1

Author(s):

Rajat Singh ◽

Utkarsh Singh ◽

Rakesh Kumar Bachheti ◽

Chandra Kiran Saini

Keyword(s):

Research Work ◽

Bioactive Compound ◽

Solvent System ◽

Antidiabetic Activity ◽

Diffusion Method ◽

Canna Indica ◽

Alpha Glucosidase ◽

Agar Well Diffusion Method ◽

In Vitro Antioxidant Activity

Objective: To investigate the antimicrobial and antidiabetic potential of canna indica plant extracts.Methods: In the present research work the selected plant i.e. Canna indica L. Cannaceae was collected, dried and extracted with different solvents. Different test were performed for the presence of different phytochemicals. The antimicrobial activity was determined by agar well-diffusion method. The extracts were evaluated for antidiabetic activity by using alpha-amylase and alpha-glucosidase enzyme inhibition method.Results and conclusion: The findings of the present study indicate that C. indica extracts contain secondary metabolites which have potent antimicrobial and antidiabetic activities comparable to standard drugs. This information may help to develop potent bioactive compound(s) in the pharmaceutical industry for the development of drugs.

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