scholarly journals Assessment of antioxidant potential of selected roadside trees leaves in Kumasi Metropolis, Ghana

2020 ◽  
Vol 7 (3) ◽  
pp. 696-701
Author(s):  
U. N. Uka ◽  
◽  
E. J. D. Belford ◽  

The roadsides of the Kumasi Metropolis, Ghana are lined with the several species of trees, such as, Terminalia catappa, Mangifera indica, Ficus platyphylla and Polyalthia longifolia. The people use them for their health care needs. The vehicle emissions results in oxidative injury in these plants, due to the production of reactive oxygen species. The present study assessed the antioxidant potential of leaves of these tree species subjected to vehicular pollutants. The free radical scavenging activity of leaf extracts of the four tree species were measured using 1, 1- diphenyl-2-picryl hydrazyl (DPPH). The total phenolic content (TPC) of the extract was determined by a spectrophotometric assay using the Folin-Ciocalteau’s reagent. Total antioxidant capacity (TAC) was measured using Phosphomolybdate assay. In this study, the medicinal properties of leaves of Terminalia catappa, Mangifera indica, Ficus platyphylla and Polyalthia longifolia sampled from the control sites showed better medicinal properties. DPPH scavenging activity at concentration 2.7 ug ml-1 was lower at the arterial road sites in all the four tree species. A higher DPPH percentage inhibition was recorded at the control sites. The IC50 values were higher for the leaf sample extracts from the arterial road sites and lower for the Control site. The total phenolic content of leaf samples of all the four tree species at the arterial road sites were lower than and significantly different from those at the Control site (p=0.000). The TAC values were lower at the arterial road sites in comparison to the control sites. There was a significant difference among the arterial road sites and also when compared with the control (P<0.05). It could be suggested from this study that variability exists in the antioxidant activities of plants due to a decrease in the medicinal properties of plants subjected to constant auto vehicular pollution.

2021 ◽  
Vol 943 (1) ◽  
pp. 012023
Author(s):  
S Nungwongsa ◽  
S Sayasoonthorn ◽  
D Jaisut ◽  
T Chungcharoen ◽  
W Thakhiew ◽  
...  

Abstract Dried slightly sticky mango sheets is healthy sweet snack, having slightly chewy texture. The objective of this work was to study the effect of drying temperatures (60, 70 and 80 °C) on the drying characteristics, total phenolic content (TPC), antioxidant activity (assessed by DPPH and ABTS scavenging activity assays) and specific energy consumption of the dried slightly sticky mango sheets. The experimental results showed that the times required to reach the desired final moisture content were 230, 150 and 120 min when drying was performed at the temperatures of 60, 70 and 80 °C, respectively. The total specific energy consumption (SECtotal) values of the drying at 70 and 80 °C were lower than that at 60 °C. However, the TPC and the antioxidant activities assessed by DPPH and ABTS scavenging activity assays were not significantly different amongst the samples dried at different temperatures.


2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nii Korley Kortei ◽  
George Tawia Odamtten ◽  
Mary Obodai ◽  
Victoria Appiah ◽  
Felicia Akuamoa ◽  
...  

Dried Pleurotus ostreatus mushrooms stored in polythene and polypropylene packs were exposed to gamma radiation from a cobalt-60 source at doses of 0, 0.5, 1, 1.5, and 2 kGy at a dose rate of 1.7 kGy/hr and stored for a period of 1 month. Total phenolic contents, flavonoids, and free radical scavenging activity DPPH (2,2′-diphenyl-1-picrylhydrazyl) were determined using aqueous, ethanol, and methanol extracts by Folin-Ciocaultaeu method as a source of potential natural antioxidants. Total phenol content ranged 0.56 ± 0.01–10.96 ± 1.7 mg/GAE, flavonoids ranged 1.64 ± 0.05–8.92 ± 0.6 mg/QE, DPPH radical scavenging activity also ranged 7.02 ± 0.10–13.03 ± 0.04%, and IC50 values also ranged 0.08–0.16 mg/mL. Statistical differences (P < 0.05) were recorded for the extracts and the treatment doses of mushrooms stored in polythene and polypropylene packs. A significant linear correlation was confirmed between values for the total phenolic content and antioxidant activity of mushroom extracts. The high contents of phenolic compounds indicated that these compounds contribute to high antioxidant activity. Pleurotus ostreatus can be regarded as a promising candidate for natural mushroom sources of antioxidants with high value. The use of low dose gamma radiation by the local food industry could improve the hygienic quality, extend shelf-life, and preserve nutrients and antinutrients.


2021 ◽  
Vol 913 (1) ◽  
pp. 012042
Author(s):  
N Ardiana ◽  
A S Abidin ◽  
B T K Ilhami ◽  
A L Sunarwidhi ◽  
S Widyastuti ◽  
...  

Abstract Seaweed is one of the marine-biota that is widely known for its uses, one of them is in the food functional. Functional food is a program that promotes a healthy diet so that it can prevent a disease when consuming food. With functional food, daily eating patterns can be maintained and also the content of the food consumed daily has a good effect on the body. It is known that in seaweed, especially brown algae (S. crassifolium), it contains antioxidants and other compounds that are good for the health. In this study, phytochemical content and proximate composition was evaluated in S. crassifolium. Including total flavonoid content and total phenolic content, antioxidant capacity both of DPPH and ABTS assay used three type of solvents such as Ethanol, n-Hexana, and ethyl acetate for comparable the best solvents. Also, proximate analysis to determine the content of moisture content, crude fat content, crude protein content, and carbohydrates content. The results showed that n-Hexana and ethyl acetate solvents were significantly than ethanol. Which mean both of them had highest values of total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity. The results of chemical composition in proximate compounds showed that S. crassifolium was suitable to be candidate of functional food.


2012 ◽  
Vol 66 (5) ◽  
pp. 723-726 ◽  
Author(s):  
Boris Pejin ◽  
Jelena Bogdanovic-Pristov

A limited number of mosses have ever been studied in regard to antioxidant activity; with only few belonging to the European species. As part of our on-going screening of bryophytes as natural antioxidant resources, a relevant biological activity investigation was conducted on the three moss species, namely Brachythecium rutabulum (Hedw.) Schimp. (Brachytheciaceae), Calliergonella cuspidata (Hedw.) Loeske (Hypnaceae) and Hypnum mammillatum (Brid.) Loeske (Hypnaceae), collected in Germany. The antioxidant activity of corresponding lyophilised aqueous extracts (teas) was evaluated on the basis of their ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)] cation scavenging activities. The total phenolic contents were determined as well. This study led to the detection of a moderate total phenolic content of the moss B. rutabulum, indicating for the first time its potential in searching for novel antioxidant agents.


Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1219 ◽  
Author(s):  
Supawadee Burapan ◽  
Mihyang Kim ◽  
Yingyong Paisooksantivatana ◽  
Bekir Engin Eser ◽  
Jaehong Han

For the functional food applications, antioxidant properties and the bioactive compounds of the 23 Curcuma species commercially cultivated in Thailand were studied. Total phenolic content and DPPH radical scavenging activity were determined. The concentrations of eight bioactive compounds, including curcumin (1), demethoxycurcumin (2), bisdemethoxycurcumin (3), 1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (4), germacrone (5), furanodienone (6), zederone (7), and ar-turmerone (8), were determined from the Curcuma by HPLC. While the total phenolic content of C. longa was highest (22.3 ± 2.4 mg GAE/g, mg of gallic acid equivalents), C. Wan Na-Natong exhibited the highest DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity. Twenty-three Curcuma species showed characteristic distributions of the bioactive compounds, which can be utilized for the identification and authentication of the cultivated Curcuma species. C. longa contained the highest content of curcumin (1) (304.9 ± 0.1 mg/g) and C. angustifolia contained the highest content of germacrone (5) (373.9 ± 1.1 mg/g). It was noteworthy that 1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (4) was found only from C. comosa at a very high concentration (300.7 ± 1.4 mg/g). It was concluded that Thai Curcuma species have a great potential for the application of functional foods and ingredients.


2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Ozahrah Hasan Basir ◽  
◽  
Nurul Atika Zaid ◽  
Noor Akhmazillah Mohd Fauzi ◽  
Aliff Hisyam A Razak ◽  
...  

Antioxidant plays a significant role in inhibiting and scavenging free radicals, which protectshuman against infection and degenerative diseases. Nowadays, most studies focus on “natural antioxidants” from herbs due to safe therapeutic. In this study, performance on different extraction methods, i.e. Soxhlet, cold maceration and traditional extraction method assisted with ultrasonic-assisted extraction (UAE) for Asystasia gangetica leaves and Phyllanthus niruri plant are investigated by means of antioxidant, total phenolic content and antibacterial activity. The extracts were characterized for its antioxidant activity and total phenolic content by means of DPPH radical scavenging method and Folin-Ciocalteau reagent respectively. Optimization studies were carried out on combined cold-maceration and UAE extraction method by using Response Surface Method. Then, the optimized extracts were further characterized for antibacterial activity using Escherichia coliand Staphylococcus epidermidisby the agar disc diffusion method. Sample containing cold macerated A. gangetica and P. niruri indicates the highest antioxidant property as it contains the highest percentage of DPPH scavenging activity (59.57% and 51.14% respectively). While, the total phenolic content of macerated A. gangetica and P. niruri are 2.73 mg GAE/g and 2.68 mg GAE/g respectively. Consequently, after the determination of highest percentage of DPPH scavenging activity, the extracts werefurther optimized using UAE. From the optimization, the optimum condition was determined to be power of 17.57±0.01W and time of 5.83±0.01minutes for A. gangetica while, the power of 98.14±0.01W and 0.17±0.01minutes for P. niruri. Both optimized extracts showed potent antibacterial activity with high degree of antibacterial against E. coli. In conclusion, combination of cold maceration and UAE method increase the amount of antioxidant from extracts of A. gangetica and P. niruri. The finding from this study can be useful in pharmaceutical and cosmeceutical industries.


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