scholarly journals Pengaruh bahan pra-sterilan, tutup tabung kultur, dan musim terhadap tingkat kontaminasi eksplan pada kultur microcutting karet Effect of pre-sterilization agent, culture tube closure, and season on the contamination level of rubber microcutting culture

2016 ◽  
Vol 77 (2) ◽  
Author(s):  
. NURHAIMI-HARIS ◽  
. SUMARYONO ◽  
M.P. CARRON CARRON
Keyword(s):  
Hevea Brasiliensis ◽  
Rainy Season ◽  
Clonal Propagation ◽  
Dry Season ◽  
Contamination Level ◽  
Culture Tube ◽  
Culture Phase ◽  
Tube Closure

AbstractMicrobial contamination is a major obstaclein clonal propagation of hevea (Heveabrasiliensis) through microcutting technology;therefore the ability to reduce contamination willdetermine the success of the application of thistechnology. The aim of experiments was toincrease healthy and survived plantlets by testingpre-sterilization agents for cleaning explantsduring pre-sterilization step, culture tubeclosures suitable for explants growth and anappropriate time for introducing explants at theprimary culture phase. The pre-sterilizationagents tested were aganol, alcohol anddesogerme, the culture tube closures used wereparafilm and cotton, and the time for culturingexplants were determined by using rubbergenotypes introduced during the year of 2006 and2007. The results show that desogermedecreased significantly the level of explantcontamination compared to aganol and alcohol,meanwhile the type of culture tube closure didnot affect the level of explant contamination. Thetype of culture tube closure influencedsignificantly the survival of explants where thenumber of survived explants in culture tubescovered with cotton was higher than that of withparafilm. Season also affected the contaminationfrequency of the explants. Higher number ofhealthy plantlets were obtained whenintroduction of the explants were conducted fromJune to October considered as dry season inBogor compared to introduction of the explantsduring rainy season from January to May.Different genotypes of rubber introduced at theprimary culture phase did not affect thepercentage of explant contamination.AbstrakKontaminasi oleh mikroba merupakanmasalah utama pada perbanyakan klonal tanamankaret (Hevea brasiliensis) melalui teknologimicrocutting sehingga kemampuan mengurangikontaminasi menentukan keberhasilan aplikasiteknologi tersebut. Penelitian ini bertujuanmempelajari pengaruh jenis bahan pra-sterilanyang efektif untuk pencucian eksplan tahap pra-sterilisasi, mempelajari pengaruh tutup tabungterhadap perkembangan eksplan serta meng-identifikasi waktu yang tepat untuk melaksanakanintroduksi eksplan pada tahap kultur primer(kultur awal) sehingga jumlah eksplan sehat dantumbuh dapat ditingkatkan. Bahan pra- sterilanyang diuji adalah aganol, alkohol dan desogerme,tutup tabung yang digunakan adalah parafilm dankapas, sedangkan identifikasi waktu kulturdilakukan melalui introduksi eksplan sepanjang tahun 2006 dan 2007 terhadap berbagai genotipetanaman karet yang tersedia. Hasil penelitianmenunjukkan bahwa desogerme menurunkansecara nyata tingkat kontaminasi eksplandibandingkan dengan aganol dan alkohol,sedangkan jenis tutup tabung tidak berpengaruhterhadap persentase kontaminasi. Jenis tutuptabung berpengaruh sangat nyata terhadappersentase eksplan yang hidup dan membentuktunas, di mana persentase eksplan membentuktunas pada tabung dengan tutup kapas lebih tinggidibandingkan dengan tutup parafilm. Musim jugasangat mempengaruhi tingkat kontaminasieksplan. Eksplan sehat jauh lebih banyakdiperoleh apabila penanaman eksplan dilakukanpada bulan Juni sampai Oktober, yang merupakanmusim kemarau di Bogor dibandingkan denganintroduksi eksplan pada bulan Januari sampaiMei, yang merupakan musim hujan. Jenisgenotipe yang ditanam pada tahap kultur primertidak berpengaruh terhadap persentasekontaminasi.

scholarly journals Pengaruh bahan pra-sterilan, tutup tabung kultur, dan musim terhadap tingkat kontaminasi eksplan pada kultur microcutting karet Effect of pre-sterilization agent, culture tube closure, and season on the contamination level of rubber microcutting culture

2016 ◽  
Vol 77 (2) ◽  
Author(s):  
. NURHAIMI-HARIS ◽  
. SUMARYONO ◽  
M.P. CARRON CARRON
Keyword(s):  
Hevea Brasiliensis ◽  
Rainy Season ◽  
Clonal Propagation ◽  
Dry Season ◽  
Contamination Level ◽  
Culture Tube ◽  
Culture Phase ◽  
Tube Closure

AbstractMicrobial contamination is a major obstaclein clonal propagation of hevea (Heveabrasiliensis) through microcutting technology;therefore the ability to reduce contamination willdetermine the success of the application of thistechnology. The aim of experiments was toincrease healthy and survived plantlets by testingpre-sterilization agents for cleaning explantsduring pre-sterilization step, culture tubeclosures suitable for explants growth and anappropriate time for introducing explants at theprimary culture phase. The pre-sterilizationagents tested were aganol, alcohol anddesogerme, the culture tube closures used wereparafilm and cotton, and the time for culturingexplants were determined by using rubbergenotypes introduced during the year of 2006 and2007. The results show that desogermedecreased significantly the level of explantcontamination compared to aganol and alcohol,meanwhile the type of culture tube closure didnot affect the level of explant contamination. Thetype of culture tube closure influencedsignificantly the survival of explants where thenumber of survived explants in culture tubescovered with cotton was higher than that of withparafilm. Season also affected the contaminationfrequency of the explants. Higher number ofhealthy plantlets were obtained whenintroduction of the explants were conducted fromJune to October considered as dry season inBogor compared to introduction of the explantsduring rainy season from January to May.Different genotypes of rubber introduced at theprimary culture phase did not affect thepercentage of explant contamination.AbstrakKontaminasi oleh mikroba merupakanmasalah utama pada perbanyakan klonal tanamankaret (Hevea brasiliensis) melalui teknologimicrocutting sehingga kemampuan mengurangikontaminasi menentukan keberhasilan aplikasiteknologi tersebut. Penelitian ini bertujuanmempelajari pengaruh jenis bahan pra-sterilanyang efektif untuk pencucian eksplan tahap pra-sterilisasi, mempelajari pengaruh tutup tabungterhadap perkembangan eksplan serta meng-identifikasi waktu yang tepat untuk melaksanakanintroduksi eksplan pada tahap kultur primer(kultur awal) sehingga jumlah eksplan sehat dantumbuh dapat ditingkatkan. Bahan pra- sterilanyang diuji adalah aganol, alkohol dan desogerme,tutup tabung yang digunakan adalah parafilm dankapas, sedangkan identifikasi waktu kulturdilakukan melalui introduksi eksplan sepanjang tahun 2006 dan 2007 terhadap berbagai genotipetanaman karet yang tersedia. Hasil penelitianmenunjukkan bahwa desogerme menurunkansecara nyata tingkat kontaminasi eksplandibandingkan dengan aganol dan alkohol,sedangkan jenis tutup tabung tidak berpengaruhterhadap persentase kontaminasi. Jenis tutuptabung berpengaruh sangat nyata terhadappersentase eksplan yang hidup dan membentuktunas, di mana persentase eksplan membentuktunas pada tabung dengan tutup kapas lebih tinggidibandingkan dengan tutup parafilm. Musim jugasangat mempengaruhi tingkat kontaminasieksplan. Eksplan sehat jauh lebih banyakdiperoleh apabila penanaman eksplan dilakukanpada bulan Juni sampai Oktober, yang merupakanmusim kemarau di Bogor dibandingkan denganintroduksi eksplan pada bulan Januari sampaiMei, yang merupakan musim hujan. Jenisgenotipe yang ditanam pada tahap kultur primertidak berpengaruh terhadap persentasekontaminasi.

STUDI BIONOMIK VEKTOR MALARIA DI KECAMATAN KALIBAWANG, KULONPROGO

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Sardjito Eko Windarso dkk
Keyword(s):  
Land Use ◽  
Malaria Vector ◽  
Rainy Season ◽  
Dry Season ◽  
Physical Factors ◽  
Mosquito Vector ◽  
Anopheles Species ◽  
Commercial Farming ◽  
Study Results ◽  
Dry And Rainy Seasons

The increasing of malaria cases in recent years at Kecamatan Kalibawang has been suspected correspond with the conversion of farming land-use which initiated in 1993. Four years after the natural vegetation in this area were changed become cocoa and coffee commercial farming estates, the number of malaria cases in 1997 rose more than six times, and in 2000 it reached 6085. This study were aimed to observe whether there were any differences in density and diversity of Anopheles as malaria vector between the cocoa and mix farming during dry and rainy seasons. The results of the study are useful for considering the appropriate methods, times and places for mosquito vector controlling. The study activities comprised of collecting Anopheles as well as identifying the species to determine the density and diversity of the malaria vector. Both activities were held four weeks in dry season and four weeks in rainy season. The mea-surement of physical factors such as temperature, humidity and rainfall were also conducted to support the study results. Four dusuns which meet the criteria and had the highest malaria cases were selected as study location. Descriptively, the results shows that the number of collected Anopheles in cocoa farming were higher compared with those in mix horticultural farming; and the number of Anopheles species identifi ed in cocoa farming were also more varied than those in the mix horticultural farming.Key words: bionomik vektor malaria, anopheles,

scholarly journals Understanding variability in greenhouse gas emission estimates of smallholder dairy farms in Indonesia

2021 ◽  
Author(s):  
Titis Apdini ◽  
Windi Al Zahra ◽  
Simon J. Oosting ◽  
Imke J. M. de Boer ◽  
Marion de Vries ◽  
...  
Keyword(s):  
Greenhouse Gas ◽  
Rainy Season ◽  
Greenhouse Gas Emission ◽  
Dairy Farms ◽  
Dry Season ◽  
Enteric Fermentation ◽  
Tropical Regions ◽  
Population Mean ◽  
Smallholder Dairy ◽  
Smallholder Dairy Farms

Abstract Purpose Life cycle assessment studies on smallholder farms in tropical regions generally use data that is collected at one moment in time, which could hamper assessment of the exact situation. We assessed seasonal differences in greenhouse gas emissions (GHGEs) from Indonesian dairy farms by means of longitudinal observations and evaluated the implications of number of farm visits on the variance of the estimated GHGE per kg milk (GHGEI) for a single farm, and the population mean. Methods An LCA study was done on 32 smallholder dairy farms in the Lembang district area, West Java, Indonesia. Farm visits (FVs) were performed every 2 months throughout 1 year: FV1–FV3 (rainy season) and FV4–FV6 (dry season). GHGEs were assessed for all processes up to the farm-gate, including upstream processes (production and transportation of feed, fertiliser, fuel and electricity) and on-farm processes (keeping animals, manure management and forage cultivation). We compared means of GHGE per unit of fat-and-protein-corrected milk (FPCM) produced in the rainy and the dry season. We evaluated the implication of number of farm visits on the variance of the estimated GHGEI, and on the variance of GHGE from different processes. Results and discussion GHGEI was higher in the rainy (1.32 kg CO2-eq kg−1 FPCM) than in the dry (0.91 kg CO2-eq kg−1 FPCM) season (P < 0.05). The between farm variance was 0.025 kg CO2-eq kg−1 FPCM in both seasons. The within farm variance in the estimate for the single farm mean decreased from 0.69 (1 visit) to 0.027 (26 visits) kg CO2-eq kg−1 FPCM (rainy season), and from 0.32 to 0.012 kg CO2-eq kg−1 FPCM (dry season). The within farm variance in the estimate for the population mean was 0.02 (rainy) and 0.01 (dry) kg CO2-eq kg−1 FPCM (1 visit), and decreased with an increase in farm visits. Forage cultivation was the main source of between farm variance, enteric fermentation the main source of within farm variance. Conclusions The estimated GHGEI was significantly higher in the rainy than in the dry season. The main contribution to variability in GHGEI is due to variation between observations from visits to the same farm. This source of variability can be reduced by increasing the number of visits per farm. Estimates for variation within and between farms enable a more informed decision about the data collection procedure.

scholarly journals Directional Distance Function Technical Efficiency of Chili Production in Thailand

2021 ◽  
Vol 13 (2) ◽  
pp. 741
Author(s):  
Wirat Krasachat ◽  
Suthathip Yaisawarng
Keyword(s):  
Distance Function ◽  
Rainy Season ◽  
Sample Selection ◽  
Agricultural Practices ◽  
Directional Distance Function ◽  
Dry Season ◽  
Small Samples ◽  
Function Technique ◽  
Security Problem ◽  
Gap Technology

To overcome the challenging food safety and security problem, in 2003, the Thai government initiated ‘Good Agricultural Practices’ (GAP) technology. This paper used a sample of 107 small chili farms from the Chiyaphoom province for the 2012 crop year, and data envelopment analysis (DEA) meta-frontier directional distance function technique to answer two questions: (1) Are GAP-adopting farms, on average, more efficient than conventional farms? (2) Does access to GAP technology affect farmers’ decisions to adopt GAP technology? We also developed an ‘indirect’ approach to reduce the potential sample selection bias for small samples. For the dry-season subsample, GAP farms were more technically efficient when compared with non-GAP farms. These dry-season non-GAP farms may not adopt the GAP method because they have limited access to GAP technology. For the rainy-season subsample, on average, GAP farms were more efficient than non-GAP farms at the 5% level. Access to the GAP technology is not a possible reason for non-GAP rainy season farms to not adopt the GAP technology. To enable sustainable development, government agencies and nongovernmental organizations (NGOs) must develop and implement appropriate educational and training workshops to promote and assist GAP technology adoption for chili farms in Thailand.

scholarly journals Application of Sentinel-1 and-2 Images in Measuring the Deformation of Kuh-e-Namak (Dashti) Namakier, Iran

2021 ◽  
Vol 13 (4) ◽  
pp. 785
Author(s):  
Sen Zhang ◽  
Qigang Jiang ◽  
Chao Shi ◽  
Xitong Xu ◽  
Yundi Gong ◽  
...  
Keyword(s):  
Synthetic Aperture Radar ◽  
Rock Salt ◽  
Rainy Season ◽  
Surface Deformation ◽  
Meteorological Data ◽  
Dry Season ◽  
Synthetic Aperture ◽  
Advantages And Disadvantages ◽  
Sbas Insar ◽  
Aperture Radar

Kuh-e-Namak (Dashti) namakier is one of the most active salt diapirs along the Zagros fold–thrust belt in Iran. Its surface deformation should be measured to estimate its long-term kinematics. Ten Sentinel-2 optical images acquired between October 2016 and December 2019 were processed by using Co-Registration of Optically Sensed Images and Correlation (COSI-Corr) method. Forty-seven Sentinel-1 ascending Synthetic Aperture Radar (SAR) images acquired between April 2017 and December 2019 were processed by using Small Baseline Subset Synthetic Aperture Radar Interferometry (SBAS-InSAR) method. The deformation of Kuh-e-Namak (Dashti) namakier was measured using both methods. Then, meteorological data were utilized to explore the relationship between the kinematics of the namakier and weather conditions and differences in macrodeformation behavior of various rock salt types. The advantages and disadvantages of COSI-Corr and SBAS-InSAR methods in measuring the deformation of the namakier were compared. The results show that: (1) The flank subsides in the dry season and uplifts in the rainy season, whereas the dome subsides in the rainy season and uplifts in the dry season. Under extreme rainfall conditions, the namakier experiences permanent plastic deformation. (2) The “dirty” rock salt of the namakier is more prone to flow than the “clean” rock salt in terms of macrodeformation behavior. (3) In the exploration of the kinematics of the namakier via the two methods, COSI-Corr is superior to SBAS-InSAR on a spatial scale, but the latter is superior to the former on a time scale.

scholarly journals Seasonal Differences in Cyclospora cayetanensis Prevalence in Colombian Indigenous People

2021 ◽  
Vol 9 (3) ◽  
pp. 627
Author(s):  
Hagen Frickmann ◽  
Juliane Alker ◽  
Jessica Hansen ◽  
Juan Carlos Dib ◽  
Andrés Aristizabal ◽  
...  
Keyword(s):  
Indigenous People ◽  
Rainy Season ◽  
Gastrointestinal Symptoms ◽  
Dry Season ◽  
Seasonal Effects ◽  
Chain Reaction ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Stool Samples ◽  
Polymerase Chain

Fecal-orally transmitted cyclosporiasis is frequent in remote resource-limited settings in Central and South America with poor hygiene conditions. In this study, we aimed at assessing seasonal effects on the epidemiology of colonization or infection with C. cayetanensis in Colombian indigenous people living under very restricted conditions. In the rainy season between July and November and in the dry season between January and April, stool samples from indigenous people with and without gastrointestinal symptoms were collected and screened for C. cayetanensis applying in-house real-time polymerase chain reaction (PCR). In the rainy season and in the dry season, positive PCR results were observed for 11.8% (16/136) and 5.1% (15/292), respectively, with cycle threshold (Ct) values of 30.6 (±3.4) and 34.4 (±1.6), respectively. Despite higher parasite loads in the rainy season, fewer individuals (2/16, 12.5%) reported gastrointestinal symptoms compared to the dry season (6/15, 40%). In conclusion, considerable prevalence of C. cayetanensis in Colombian indigenous people persists in the dry season. Low proportions of gastrointestinal symptoms along with higher parasite loads make colonization likely rather than infection.

scholarly journals Nocturnal Boundary Layer Erosion Analysis in the Amazon Using Large-Eddy Simulation during GoAmazon Project 2014/5

Atmosphere ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 240
Author(s):  
Rayonil Carneiro ◽  
Gilberto Fisch ◽  
Theomar Neves ◽  
Rosa Santos ◽  
Carlos Santos ◽  
...  
Keyword(s):  
Boundary Layer ◽  
Large Eddy Simulation ◽  
Rainy Season ◽  
Nocturnal Boundary Layer ◽  
Enso Event ◽  
Dry Season ◽  
Dry Period ◽  
Additional Energy ◽  
Eddy Simulation ◽  
Large Eddy

This study investigated the erosion of the nocturnal boundary layer (NBL) over the central Amazon using a high-resolution model of large-eddy simulation (LES) named PArallel Les Model (PALM) and observational data from Green Ocean Amazon (GoAmazon) project 2014/5. This data set was collected during four intense observation periods (IOPs) in the dry and rainy seasons in the years 2014 (considered a typical year) and 2015, during which an El Niño–Southern Oscillation (ENSO) event predominated and provoked an intense dry season. The outputs from the PALM simulations represented reasonably well the NBL erosion, and the results showed that it has different characteristics between the seasons. During the rainy season, the IOPs exhibited slow surface heating and less intense convection, which resulted in a longer erosion period, typically about 3 h after sunrise (that occurs at 06:00 local time). In contrast, dry IOPs showed more intensive surface warming with stronger convection, resulting in faster NBL erosion, about 2 h after sunrise. A conceptual model was derived to investigate the complete erosion during sunrise hours when there is a very shallow mixed layer formed close to the surface and a stable layer above. The kinematic heat flux for heating this layer during the erosion period showed that for the rainy season, the energy emitted from the surface and the entrainment was not enough to fully heat the NBL layer and erode it. Approximately 30% of additional energy was used in the system, which could come from the release of energy from biomass. The dry period of 2014 showed stronger heating, but it was also not enough, requiring approximately 6% of additional energy. However, for the 2015 dry period, which was under the influence of the ENSO event, it was shown that the released surface fluxes were sufficient to fully heat the layer. The erosion time of the NBL probably influenced the development of the convective boundary layer (CBL), wherein greater vertical development was observed in the dry season IOPs (~1500 m), while the rainy season IOPs had a shallower layer (~1200 m).

scholarly journals Seasonal influence of tuberculosis diagnosis in Rwanda

2021 ◽  
Vol 49 (1) ◽  
Author(s):  
Doris Uwamahoro ◽  
Aly Beeman ◽  
Vinay K. Sharma ◽  
Michael B. Henry ◽  
Stephanie Chow Garbern ◽  
...  
Keyword(s):  
Rainy Season ◽  
Laboratory Data ◽  
Retrospective Chart Review ◽  
Dry Season ◽  
University Teaching ◽  
Health Concern ◽  
People Living With Hiv ◽  
Hiv Status ◽  
Tropical Country ◽  
Included Patient

Abstract Background Tuberculosis (TB) remains a major global health concern. Previous research reveals that TB may have a seasonal peak during the spring and summer seasons in temperate climates; however, few studies have been conducted in tropical climates. This study evaluates the influence of seasonality on laboratory-confirmed TB diagnosis in Rwanda, a tropical country with two rainy and two dry seasons. Methods A retrospective chart review was performed at the University Teaching Hospital-Kigali (CHUK). From January 2016 to December 2017, 2717 CHUK patients with TB laboratory data were included. Data abstracted included patient demographics, season, HIV status, and TB laboratory results (microscopy, GeneXpert, culture). Univariate and multivariable logistic regression (adjusted for age, gender, and HIV status) analyses were performed to assess the association between season and laboratory-confirmed TB diagnoses. Results Patients presenting during rainy season periods had a lower odds of laboratory-confirmed TB diagnosis compared to the dry season (aOR=0.78, 95% CI 0.63–0.97, p=0.026) when controlling for age group, gender, and HIV status. Males, adults, and people living with HIV were more likely to have laboratory-confirmed TB diagnosis. On average, more people were tested for TB during the rainy season per month compared to the dry season (120.3 vs. 103.3), although this difference was not statistically significant. Conclusion In Rwanda, laboratory-confirmed TB case detection shows a seasonal variation with patients having higher odds of TB diagnosis occurring in the dry season. Further research is required to further elucidate this relationship and to delineate the mechanism of season influence on TB diagnosis.

scholarly journals Seasonal Variation of Rainy and Dry Season Per Capita Water Consumption in Freetown City Sierra Leone

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 499
Author(s):  
Salmatta Ibrahim A ◽  
Fayyaz Ali Memon ◽  
David Butler
Keyword(s):  
Seasonal Variation ◽  
Water Use ◽  
Water Consumption ◽  
Low Income ◽  
Rainy Season ◽  
Predictor Variable ◽  
Dry Season ◽  
Data Set ◽  
Per Capita ◽  
End Use

Ensuring a sustainable urban water supply for developing/low-income countries requires an understanding of the factors affecting water consumption and technical evidence of individual consumption which can be used to design an improved water demand projection. This paper compared dry and rainy season water sources available for consumption and the end-use volume by each person in the different income groups. The study used a questionnaire survey to gather household data for a total of 398 households, which was analysed to develop the relationship between per capita water consumption characteristics: Socio-economic status, demographics, water use behaviour around indoor and outdoor water use activities. In the per capita water consumption patterns of Freetown, a seasonal variation was found: In the rainy season, per capita water consumption was found to be about 7% higher than the consumption for the full sample, whilst in the dry season, per capita water consumption was almost 14% lower than the full survey. The statistical analysis of the data shows that the average per capita water consumption for both households increases with income for informal slum-, low-, middle- and high-income households without piped connection (73, 78, 94 and 112 L/capita/day) and with connection (91, 97, 113 and 133 L/capita/day), respectively. The collected data have been used to develop 20 statistical models using the multiple linear stepwise regression method for selecting the best predictor variable from the data set. It can be seen from the values that the strongest significant relationships of per capita consumption are with the number of occupants (R = −0.728) in the household and time spent to fetch water for use (R = −0.711). Furthermore, the results reveal that the highest fraction of end use is showering (18%), then bathing (16%), followed by toilet use (14%). This is not in agreement with many developing countries where toilet use represents the largest component of indoor end use.

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