scholarly journals Asosiasi Glomus sp. dan Gigaspora margarita pada bibit Aquilaria malaccensis

2019 ◽  
Vol 87 (2) ◽  
Author(s):  
Endah Susilowati ◽  
Melya Riniarti ◽  
Maria Viva Rini

AbstractAgarwood (Aquilaria malaccensis) is one of the non-timber forest products that has high economic value. However, this plant grows very slowly.  Therefore, seed technology input is needed, such as addition of arbuscular mycorrhizal fungi (AMF). The objectives of this research were to study the effect of AMF inoculum i.e. Glomus sp., Gigaspora margarita and mixture of both species on agarwood growth and to determine the best AMF type for agarwood seedlings growth. The experimental research design used was a completely randomized design with three treatments, namely Glomus sp. (G), G. margarita (Gi) and a mixture of Glomus sp. and G. margarita (GGi) and without AMF inoculation (K) with each treatment repeated 8 times. The number of AMF spores used was ± 300 spores/seedling, and applied at the time of transplanting from germination tray to the polybag. Data were processed using analysis of variance and least significant difference test (LSD). The results showed there was AMF colonization in agarwood roots after 12 weeks of inoculation with the highest percentage at 20.50% on G. margarita treatment. Overall, AMF improved the growth of agarwood seedlings. The best seedlings growth was in the treatment of mixed AMF (GGi). Increased growth was found in plant height (14.68 cm), seedling diameter (2.16 mm), leaf area (119.30 cm2), root volume (1.15 mL), total dry weight (0.83 g), and shoot root ratio (4.99).[Keywords: agarwood, A. malaccensis,AMF, G. margarita,Glomussp.]AbstrakGaharu (Aquilariamalaccensis) adalah salah satu hasil hutan bukan kayu yang memiliki nilai ekonomis tinggi. Namun tanaman ini memiliki pertumbuhan yang lambat, sehingga diperlukan input teknologi pembibitan berupa penambahan fungi mikoriza arbuskular (FMA). Penelitian bertujuan untuk mengetahui pengaruh pemberian FMA tunggal Glomussp., Gigaspora margaritadan campuran keduanya (Glomussp. dan G. margarita)terhadap pertumbuhan gaharu serta menentukan isolatyang menghasilkan pertumbuhan bibit gaharu yang lebih baik.  Rancangan percobaan yang digunakan adalah rancangan acak lengkap untuk menguji tiga perlakuan yaitu Glomussp. (G), G. margarita (Gi) dan campuran Glomus sp. dan G. margarita(GGi) serta satu kontrol (K) dengan  masing – masing perlakuan diulang 8 kali. Inokulum FMA yang digunakan memiliki kepadatan ±300 spora/bibit dan diinokulasikan saat bibit dipindahkan dari persemaian ke polybag. Data diolah menggunakan analisis varians dan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa ditemukan adanya kolonisasi FMA dalam akar gaharu setelah 12 minggu inokulasi dengan persentase kolonisasi tertinggi 20,50% oleh G. margarita.  Secara keseluruhan, pemberian FMA mampu meningkatkan pertumbuhan bibit gaharu. Pertumbuhan terbaik terjadi pada perlakuan FMA campuran (GGi).  Peningkatan pertumbuhan secara nyata dapat dilihat pada tinggi tanaman (14,68 cm), diameter bibit (2,16 mm), luas daun (119,30 cm2), volume akar (1,15 mL), bobot kering total (0,83 g) dan nisbah tajuk akar (4,99).[Kata kunci: A. malaccensis, FMA, gaharu, G. margarita, Glomus sp.]

2020 ◽  
Vol 17 (4) ◽  
pp. 150
Author(s):  
O. TRISILAWATI

<p>ABSTRACT</p><p>The effects of several arbuscular mycorrhizal fungi (AMF) on thegrowth, nutrient uptake (nitrogen, phosphorus, and potassium), and acidphosphate activity of two promising numbers of Anacardium occidentaleseedling were evaluated. The experiment was conducted in the greenhouse of Indonesian Spices and Medicinal Crops Research Institute(BALITTRO) in 2002 for six months on a randomized design with twofactors and four replicates. First factor was isolate (six isolates of AMFand one control) consisting of : 1) control; 2) Glomus aggregatum; 3)Glomus etunicatum; 4) Mycofer; 5) Glomus sp.; 6) a mixture of Glomussp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomus etunicatum,Gigaspora margarita, Gigaspora sp., and Enthropospora sp., and 7)Gigaspora sp. The second factor was two cashew promising numbers :Asembagus and Wonogiri. The results showed that AMF inoculationsignificantly affected the growth of cashew. Mycofer and mixed AMFwere more effective to Wonogiri promising number, while for Asembaguspromising number inoculation of mycofer was more effective. Inoculationwith mycofer to Asembagus promising number increased the uptake of Pand K nutrients by 65 and 53% while inoculation with mycofer and mixedAMF to Wonogiri promising number increased the uptake of N, P and Knutrients by 55, 38, and 17%, and by 18, 31, and 17%. Moreover, theAMF inoculation resulted in higher phosphatase activity. In mycorrhizalAsembagus promising number infected by mixed AMF, the increment ofphosphatase activity was 136.5%, whether in Wonogiri promising numberinfected by mycofer, the increment of phosphatase activity was 80% thancontrol.</p><p>Key words: Anacardium occidentale, promising number, growth,phosphatase activity</p><p>ABSTRAK</p><p>Pengaruh Pupuk Hayati Fungi Mikoriza Arbuskula(FMA) terhadap Pertumbuhan Benih Jambu Mete</p><p>Penelitian ini bertujuan untuk mengetahui pengaruh beberapa jenisfungi mikoriza arbuskula (FMA) terhadap pertumbuhan, serapan hara danaktivitas enzim fosfatase dari dua nomor harapan benih jambu mete(Anacardium occidentale). Penelitian dilakukan di rumah kaca Balittropada tahun 2002 selama 6 bulan, menggunakan rancangan acak yangterdiri dari dua faktor dan diulang empat kali. Faktor pertama adalahisolate (6 jenis isolat FMA dan satu kontrol) yaitu: 1). kontrol; 2). Glomusaggregatum; 3) Glomus etunicatum; 4). Mycofer; 5). Glomus sp.; 6).campuran dari Glomus sp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomusetunicatum, Gigaspora margarita, Gigaspora sp., Enthropospora sp., dan7). Gigaspora sp. Faktor kedua adalah nomor harapan jambu mete, yaituAsembagus dan Wonogiri. Hasil penelitian mendapatkan bahwa inokulasiFMA berpengaruh nyata terhadap pertumbuhan jambu mete. Mycofer dancampuran FMA lebih efektif berpengaruh terhadap nomor harapanWonogiri, sedangkan mycofer lebih efektif berpengaruh terhadap nomorharapan Asembagus. Serapan hara P dan K pada nomor harapanAsembagus yang diinokulasi mycofer meningkat sebesar 65 dan 53%,sedangkan nomor harapan Wonogiri yang diinokulasi mycofer dancampuran FMA, serapan hara N, P, dan K meningkat masing-masingsebesar 55; 38; dan 17%, dan 18; 31; dan 17%. Selain itu, inokulasi FMAdapat meningkatkan aktivitas fosfatase akar jambu mete. Peningkatanaktivitas fosfatase akar jambu mete nomor harapan Asembagus yangterinfeksi oleh campuran FMA sebesar 136,5%, sedangkan pada nomorharapan Wonogiri yang terinfeksi mycofer, peningkatnnya sebesar 80%dibandingkan kontrol.</p><p>Kata kunci: Anacardium occidentale, nomor harapan, pertumbuhan,aktivitas fosfatase</p>


2017 ◽  
Vol 12 (4) ◽  
pp. 486
Author(s):  
Ana Carolina da Silva Ardito ◽  
Orivaldo José Saggin Junior ◽  
Rosalba Ortega Fors ◽  
Rogério Sebastião Correa da Costa ◽  
Eliane Maria Ribeiro da Silva

No Brasil a cafeicultura se expande para regiões mais quentes com a cultivar clonal Conilon ‘BRS Ouro Preto’ da espécie C. canephora. A resposta da ‘BRS Ouro Preto’ à inoculação com fungos micorrízicos arbusculares (FMAs) ainda não foi testada. O presente estudo visou selecionar espécies de FMAs promotoras do crescimento e nutrição fosfatada da referida cultivar. Avaliaram-se três clones (M057, M194 e M199) em três Argissolos Vermelho-Amarelos, coletados em áreas sob: cana-de-açúcar (Solo 1), Cerrado (Solo 2) e Mata Atlântica (Solo 3). Os tratamentos de inoculação foram: Acaulospora colombiana+Glomus sp., Acaulospora scrobiculata, Claroideoglomuse tunicatum+Glomus sp., Dentiscutata heterogama, Gigaspora margarita e Rhizophagus clarus, além de duas testemunhas não inoculadas, uma delas com adubação completa. O primeiro e o terceiro solos proporcionaram maior desenvolvimento vegetativo às plantas. O teor de clorofila e fósforo nas folhas foi maior nas plantas do solo 3. A melhor resposta à inoculação, expressa através do crescimento e nutrição das plantas, foi verificada no solo 1, sendo os melhores tratamentos C. etunicatum+ Glomus sp. e G. margarita. A espécie Glomus sp., presente nos tratamentos A. colombiana + Glomus sp. e C. etunicatum+Glomus sp., apresentou alta esporulação e promoveu o crescimento e nutrição da ‘BRS Ouro Preto’. Os clones M057 e M194 apresentaram maior crescimento, resposta à inoculação e esporulação de FMAs, comparados ao clone M199. Assim, os clones M057 e M194 podem ser considerados micotróficos e promotores da esporulação, enquanto o clone M199 caracteriza-se por baixa micotrofia.


2012 ◽  
Vol 17 (2) ◽  
pp. 140 ◽  
Author(s):  
Urley Adrian Pérez-Moncada ◽  
María Margarita Ramírez-Gómez ◽  
Víctor Manuel Núñez-Zarante ◽  
Marcela Franco-Correa ◽  
Gabriel Roveda-Hoyos

<strong>Objective</strong>. Obtain an in vitro mycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). <strong>Materials and methods</strong>. We used spores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF) Glomus sp (GEV02). We established an autotrophic culture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of spores produced, the length of the extraradical mycelium as well as the percentage of colonization of the AMF. Additionally, we measured the shoot and root length, and the fresh and dry weight of the leaf and root parts to determine the plant development. <strong>Results</strong>. The autotrophic culture system was successful for blackberry plants (Rubus glaucus, Benth; an optimal shoot and root growth was observed. Additionally, we obtained a system that allowed the development of Glomus sp. in in vitro conditions, with the formation of structures typical of the symbiosis as well as a good intraradical colonization, with the production of arbuscules and vesicles, development of extraradical mycelium with branched hyphae, and formation of new spores. <strong>Conclusion</strong>. For the first time, micropropagated blackberry plants associated successfully with an AMF under in vitro conditions, enabling the development of the symbiotic system AMF Glomus sp. associated to roots of micropropagated blackberry plantlets.<br /><strong>Key words</strong>: arbuscular mycorrhizal fungi (AMF), autotrophic culture, Rubus glaucus Benth, Glomus sp. (GEV02), in vitro mycorrhization.


2021 ◽  
Vol 26 (1) ◽  
pp. 19
Author(s):  
Slamet Budi Yuwono ◽  
Alawiyah Alawiyah ◽  
Melya Riniarti ◽  
Dermiyati Dermiyati

Rehabilitation of post-mining limestone soils is often a challenge due to a lack of nutrients and poor soil humus. The research aims to study the effect of various ameliorants on soil chemical properties, growth, and P uptake of gaharu plant (Aquilaria malaccensis) in post-mining limestone soil for revegetation of critical land. The research was conducted in a Completely Randomized Design with three replications. The treatments were P0 (Control, without ameliorant); P1 (Humic Acid, HA, 4 kg ha-1 ); P2 (Phosphate Rocks, PR, 350 kg ha-1 ); P3 (Vesicular Arbuscular Mycorrhizal Fungi, AMF, 500 spores plant-1 ); P4 (HA+AMF); P5 (PR+AMF); and P6 (HA+PR+AMF). Data were analyzed using ANOVA at 95% confidence level and continued with the LSD test. The use of various types of ameliorants (HA, RP, and AMF) significantly increased root length, root volume, wet and dry weight of roots, shoot wet and dry weight, and P uptake of gaharu tree. The best ameliorant in increasing gaharu tree growth was AMF (P3) treatment, and AMF combined with HA (P4) treatment. Thus, for revegetation of critical land, especially post-mining limestone land, using gaharu tree requires HA and AMF inoculation.


2018 ◽  
Vol 8 (3) ◽  
pp. 147-153
Author(s):  
Hutami Indah Pertiwi ◽  
Sri Wilarso Budi R. ◽  
Arum Sekar Wulandari

Jabon (Anthocepalus cadamba Roxb.) is one of the fast-growing species that naturally spread in some areas of Indonesia. Known as well adapted to some types of soil, and attributes to a quite high of economical prospects value. Interaction of Arbuscular Mycorrhiza Fungi (AMF) symbiotic involve not only between fungi and plant’s root but also involving supporting organisms (bacteria). This bacteria capable to stimulating the development of mycorrhizal hyphae namely as Mycorrhiza Helper Bacteria (MHB). The aims of this research was to discover of bacterial isolates that can stimulate the development of AMF in Jabon seedling and to examine the effectiveness of MHB isolate and AMF toward Jabon growth. The experiment was conducted in Completely Randomized-split splot design with two factors. The main plot was AMF with two levels; without AMF (M0) and with AMF respectively. Bacteria as the sub-plot with 19 levels consist of the control (B0), Isolate of Glomus sp with coding B1, B2, B3, B4, B5, B6, B7, B8, B9, B10, B11, B12, B13, B14, B15, B16, B17, and B18 respectively. Our experiment result showed that AMF inoculated of Jabon seedling had significantly effect on root colonization and root dry weight. The average of root colonization was 20.2%. Root dry weight increased 4.69% compared to control. Bacteria were suspected as MHB has not provided significant resultsKey words: arbuscular mycorrhizal fungi, jabon, mycorrhizal helper bacteria


2017 ◽  
Vol 11 (1) ◽  
pp. 76 ◽  
Author(s):  
Faisal Danu Tuheteru ◽  
Asrianti Arif ◽  
Eka Widiastuti ◽  
Ninis Rahmawati

Pengaruh fungi mikoriza arbuskula (FMA) lokal terhadap pertumbuhan dan serapan logam tanaman Nauclea orientalis L., telah diteliti. Tanaman ditumbuhkan pada kondisi rumah kaca pada media serpentine soil tanpa dan dengan FMA (Glomus sp., Acaulospora tuberculata, dan campuran) selama 2 bulan. Akar tanaman lonkida terkolonisasi FMA dengan ditemukan struktur FMA berupa hifa internal>hifa eksternal>coil> vesikula>arbuskula. Kolonisasi A. tuberculata dan Glomus sp. signifikan meningkatkan berat kering akar (P<0,01, r=0,810) dan pucuk (P<0,05, r=0,802). N. orientalis memiliki ketergantungan tinggi terhadap inokulasi FMA (MIE>65). Nilai Transpor Faktor (TF) <1 untuk semua logam dengan urutan serapan logam Fe>Mn>Ni>Cr. Glomus sp mengurangi serapan Fe dan Ni akar sebesar 13% dan 3%, secara berturutan. A. tuberculata meningkatkan serapan semua logam. Kemampuan serapan logam berbeda antara jenis FMA.Heavy Metal Uptake by Indigenous Arbuscular Mycorrhizas of Nauclea orientalis L. and the Potential for Phytoremediation of Serpentine SoilAbstractEffect of indigenous arbuscular mycorrhizal fungi (AMF) on growth and metal uptake of Nauclea orientalis L. plants, has been investigated. Plants were grown in greenhouse conditions on serpentine soil media without and with the AMF (Glomus sp., Acaulospora tuberculata, and mix) for two months. Lonkida roots was colonized by AMF because it was found structures of AMF: internal hyphae>external hypae>coil>vesicles>arbuscule. Colonization A. tuberculata and Glomus sp. significantly increased dry weight of root (P<0.01, r=0.810) and shoot (P<0.05, r=0.802). N. orientalis has a high dependence on inoculation of AMF (MIE>65). Transport Factor value (TF) <1 for all metals with metal uptake sequence was Fe>Mn>Ni>Cr. Glomus sp reduced Fe and Ni uptake on roots by 13% and 3%, respectively. A. tuberculata increased the uptake of all metals. Metal uptake ability was difference among types of AMF.


1998 ◽  
Vol 28 (1) ◽  
pp. 150-153
Author(s):  
J N Gemma ◽  
R E Koske ◽  
E M Roberts ◽  
S Hester

Rooted cuttings of Taxus times media var. densiformis Rehd. were inoculated with the arbuscular mycorrhizal fungi Gigaspora gigantea (Nicol. & Gerd.) Gerd. & Trappe or Glomus intraradices Schenck and Smith and grown for 9-15 months in a greenhouse. At the completion of the experiments, leaves of inoculated plants contained significantly more chlorophyll (1.3-4.1 times as much) than did noninoculated plants. In addition, mycorrhizal plants had root systems that were significantly larger (1.3-1.4 times) and longer (1.7-2.1 times) than nonmycorrhizal plants, and they possessed significantly more branch roots (1.3-2.9 times). No differences in stem diameter and height or shoot dry weight were evident at the end of the experiments, although the number of buds was significantly greater in the cuttings inoculated with G. intraradices after 15 months.


2017 ◽  
Vol 8 (1) ◽  
pp. 20-25
Author(s):  
Fransisca Yosina Regina Luturmas ◽  
Sri Wilarso Budi R. ◽  
Irdika Mansur

Jabon (Anthocephallus cadamba Roxb.) is one of fast-growing species with high economical value, and well adapted on some of soil types. The aims of this research were to analyze the effectiveness of AMF species isolated from Samama (Anthocephallus macrophyllus (Roxb.) Havil) and to determine the favorable dose of nitrogen and phosphate fertilizer for Jabon growth. The research was conducted CRD-split plot design main plot was Acaulospora sp.1 (M1), Glomus sp.1 (M2), Acaulospora sp.2 (M3), Acaulospora sp.3 (M4), Glomus sp.2 (M5). While the fertilizer as the sub-plot, consist of control (P0), urea 0.5g+rockphosphat 2g (P1), urea 1g+rockphosphat 4g (P2). The result showed that AMF and fertilizer applications effectively improved Jabon grown, especially in height, stem diameter, and shoot dry weight. The interaction (M4P1) increased plant height 154.73%, diameter 75.38% and shoot dry weight 376.09% compared with control. P1 treatment was better for growth of Jabon that was inoculated by mycorrhiza. Acaulospora sp.1 (M1) originally from Samama without fertilizer had the best effectiveness for Jabon growth.Key words: Acaulospora, indigenous


Author(s):  
Priyanka Kumari ◽  
R. R. Singh ◽  
Ruby Rani ◽  
Mahendra Singh ◽  
Uday Kumar

Litchi (Litchi chinensis Sonn.) originated from South China, it is sub-tropical evergreen fruit crops, especially grown on the marginal climate of tropics and subtropics. It is delicious juicy fruit of India having excellent nutritional quality, pleasant flavoured, good amount of antioxidant and vitamins C, vitamin B-complex and phytonutrients flavonoids. It has a great potential to earn foreign exchange in the national and international market through export. Arbuscular mycorrhizal (AM) infection is a common association between plant roots and microorganisms. It is responsible for increasing plant nutrient uptake and also increases in macro and micronutrients in leaf. Therefore, the present work has been analyzed macro and micro nutrients from soil and leaf, after 60, 90 and 120 days after inoculation of two bio-inoculants with phosphorus (SSP) including nine treatments with three replications. After 120 days of inoculation both the species of mycorrhizal combination with phosphorus application were very effective. Highest Copper content is (10.99 ppm), Zinc (33.17 ppm), Iron (121.47 ppm) and Manganese (15.33 ppm) was recorded in case T5 (G. mosseae 10 g + Phosphorus 50 mg kg-1 of soil) which is gradually increases. The soil nutrient content gradually decreased with time duration but no- significant difference was found among treatments after 120 days inoculation. After 120 days potting result was found that the Copper content is (1.70 ppm), Zinc (3.07 ppm), Iron (7.80 ppm) and Manganese (4.00 ppm) was recorded in case T5 (G. mosseae 10 g + Phosphorus 50 mg kg-1 of soil).this research was undertaken to find out whether Arbuscular mycorrhizal (AM) infection and phosphorus affect the micro-nutrient status of soil and leaves in nursery stage.


2010 ◽  
Vol 56 (No. 9) ◽  
pp. 412-418 ◽  
Author(s):  
M.C. Pagano ◽  
M.N. Cabello ◽  
M.R. Scotti

This paper focuses on Eremanthus incanus Less. (Asteraceae), a common species of highland regions in Brazil. The effect of arbuscular mycorrhizal (AM) inoculation on plant growth (height and diameter) was evaluated. Roots were examined from individuals randomly selected from undisturbed areas of highland vegetation and from an experimental restored site. Results showed that E. incanus presented high AM colonization both in restored and undisturbed sites. Moreover, AM colonization was significantly higher in the inoculated treatment than in the non-inoculated one. The species presented Arum-type colonization and frequent production of vesicles, especially in the restored site. Arbuscular mycorrhizal inoculation stimulated plant growth (height and diameter). Ten AM fungi (AMF) taxa were found in the studied rooting zones: Acaulospora spinosa, A. elegans, A. foveata, Acaulospora sp., Gigaspora margarita, Glomus sp., Dentiscutata biornata, D. cerradensis, Dentiscutata sp. and Racocetra verrucosa. These results revealed that AMF is a common and important component in highland vegetation in Brazil, and should be included in future restoration programs.


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