scholarly journals Aktivitas antibakteri ekstrak kulit buah kakao (Theobroma cacao L.) terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus aureus

2016 ◽  
Vol 80 (2) ◽  
Author(s):  
Agustin Sri MULYATNI ◽  
Asmini BUDIANI ◽  
Darmono TANIWIRYONO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Theobroma Cacao ◽  
Diffusion Method ◽  
Dry Bean ◽  
E Coli ◽  
Randomized Design ◽  
Theobroma Cacao L

AbstractCocoa (Theobroma cacao L.), one of the most important export commodities from Indonesia, is widely planted with current total area of 1.6 million Ha, producing 500.000 metric tons of dry bean  in 2011 . At the time of harvest, instead of seed approximately the same volume cacao husk is produced. The aim of the study was to assess the potential of cocoa husk extract as an antibacterial against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus, and to determine the minimum inhibitory concentration (MIC) of cocoa husk extract to the three test bacteria. Extraction of cocoa husk conducted by maceration method using ethanol 96%. Analysis of antibacterial activity was done by paper disc diffusion method. Completely Randomized Design of single factor presentage that is extract concentration of 0; 1; 2; 4; 8; 16; 32; and 64% (g/mL) with three replicans were applied.The results showed that the extract of cocoa pod husk has antibacterial activity against S. aureus, B. subtilis, and E. coli with the MIC are 8% (g/ mL), 16% (g/ mL), and 32% (g/ mL) respectively.AbstrakKakao (Theobroma cacao L.), salah satu komoditi ekspor terpenting Indonesia, ditanam secara luas dengan total luasan 1,6 juta Ha, menghasilkan 500.000 ton biji kering pada tahun 2011. Di samping biji sebagai hasil utama, pada saat panen juga dihasilkan kulit buah dengan volume yang hampir sama dengan biji. Penelitian ini bertujuan untuk mengkaji potensi ekstrak kulit buah kakao sebagai antibakteri terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus aureusserta menentukan konsentrasi hambat minimum (KHM) ekstrak kulit buah kakao terhadap ketiga bakteri uji. Ekstraksi kulit buah kakao dilakukan dengan metode Maserasi menggunakan pelarut etanol 96%. Analisis aktivitas antibakteri dilakukan dengan metode difusi cakram  kertas. Penelitian  ini  menggunakan  Rancangan Acak  Lengkap (RAL) dengan faktor tunggal  konsen-trasi ekstrak, yaitu 0; 1; 2; 4; 8; 16; 32; dan 64% (g/mL), masing-masing dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa ekstrak kulit buah kakao berpotensi sebagai antibakteri terhadap S. aureus, B. subtilis dan  E. coli, dengan KHM berturut-turut adalah 8% (g/mL), 16% (g/mL), dan 32% (g/mL).

scholarly journals Aktivitas antibakteri ekstrak kulit buah kakao (Theobroma cacao L.) terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus aureus

2016 ◽  
Vol 80 (2) ◽  
Author(s):  
Agustin Sri MULYATNI ◽  
Asmini BUDIANI ◽  
Darmono TANIWIRYONO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Theobroma Cacao ◽  
Diffusion Method ◽  
Dry Bean ◽  
E Coli ◽  
Randomized Design ◽  
Theobroma Cacao L

AbstractCocoa (Theobroma cacao L.), one of the most important export commodities from Indonesia, is widely planted with current total area of 1.6 million Ha, producing 500.000 metric tons of dry bean  in 2011 . At the time of harvest, instead of seed approximately the same volume cacao husk is produced. The aim of the study was to assess the potential of cocoa husk extract as an antibacterial against Escherichia coli, Bacillus subtilis, and Staphylococcus aureus, and to determine the minimum inhibitory concentration (MIC) of cocoa husk extract to the three test bacteria. Extraction of cocoa husk conducted by maceration method using ethanol 96%. Analysis of antibacterial activity was done by paper disc diffusion method. Completely Randomized Design of single factor presentage that is extract concentration of 0; 1; 2; 4; 8; 16; 32; and 64% (g/mL) with three replicans were applied.The results showed that the extract of cocoa pod husk has antibacterial activity against S. aureus, B. subtilis, and E. coli with the MIC are 8% (g/ mL), 16% (g/ mL), and 32% (g/ mL) respectively.AbstrakKakao (Theobroma cacao L.), salah satu komoditi ekspor terpenting Indonesia, ditanam secara luas dengan total luasan 1,6 juta Ha, menghasilkan 500.000 ton biji kering pada tahun 2011. Di samping biji sebagai hasil utama, pada saat panen juga dihasilkan kulit buah dengan volume yang hampir sama dengan biji. Penelitian ini bertujuan untuk mengkaji potensi ekstrak kulit buah kakao sebagai antibakteri terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus aureusserta menentukan konsentrasi hambat minimum (KHM) ekstrak kulit buah kakao terhadap ketiga bakteri uji. Ekstraksi kulit buah kakao dilakukan dengan metode Maserasi menggunakan pelarut etanol 96%. Analisis aktivitas antibakteri dilakukan dengan metode difusi cakram  kertas. Penelitian  ini  menggunakan  Rancangan Acak  Lengkap (RAL) dengan faktor tunggal  konsen-trasi ekstrak, yaitu 0; 1; 2; 4; 8; 16; 32; dan 64% (g/mL), masing-masing dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa ekstrak kulit buah kakao berpotensi sebagai antibakteri terhadap S. aureus, B. subtilis dan  E. coli, dengan KHM berturut-turut adalah 8% (g/mL), 16% (g/mL), dan 32% (g/mL).

Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

2020 ◽  
Vol 15 (6) ◽  
pp. 665-679
Author(s):  
Alok K. Srivastava ◽  
Lokesh K. Pandey
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

2017 ◽  
Vol 901 ◽  
pp. 124-132
Author(s):  
Artania Adnin Tri Suma ◽  
Tutik Dwi Wahyuningsih ◽  
Deni Pranowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Shigella Flexneri ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (COFFEA CANEPHORA L.) LEAVES TO STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2019 ◽  
pp. 113-115
Author(s):  
ZAMHARIRA MUSLIM ◽  
YONANIKO DEPHINTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Effect ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Robusta Coffee

Objective: This research aims to analyze the ability of robusta coffee leaves fraction extract to inhibit the growth of Staphylococcus aureus and Escherichia coli and also determine the minimum inhibitory concentration (MIC). Methods: Antibacterial activity evaluated by the disc diffusion method observed in four types of fraction of extract robusta coffee leaves (n-hexane, ethyl acetate, ethanol, and water). Each extract divided into three various concentrations, 5%, 10%, and 15%. Determination of antimicrobial activity in vitro by the disk diffusion method. Results: Ethyl acetate fraction of coffee leaves extract produced the largest diameter zone of inhibition of bacterial growth compared to other extraction fractions of 17.28 mm in E. coli and 18.58 mm in S. aureus. The MIC of coffee leaves extract fraction water, ethyl acetate, and n-hexane on E. coli and S. aureus is 5%, while the fraction ethanol MIC is 10%. Conclusion: The antibacterial effect of ethyl acetate fraction of coffee leaves extract showed an antibacterial effect that was better than the fraction of n-hexane, ethanol, and water.

Bakteri Simbion Karang Porites dari Perairan Gunungkidul, Yogyakarta dan Aktivitas Antibakteri terhadap Bakteri Patogen Staphylococcus aureus dan Escherichia coli

2018 ◽  
Vol 7 (1) ◽  
pp. 43
Author(s):  
Rivan Novianto Madilana ◽  
Diah Permata Wijayanti ◽  
Agus Sabdono
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
16S Rrna ◽  
Bacillus Pumilus ◽  
Coral Tissue ◽  
Diffusion Method ◽  
Rrna Gene ◽  
E Coli ◽  
Porites Coral

Porites merupakan genus karang yang memiliki persebaran luas di Indonesia, termasuk perairan Gunungkidul, Yogyakarta. Penelitian menunjukkan bahwa bakteri simbion karang Porites memiliki potensi antibakteri dalam menanggulangi bakteri patogen. Penelitian ini betujuan untuk mengetahui jenis bakteri simbion karang Porites dari Perairan Gunungkidul Yogyakarta yang memiliki aktivitas antibakteri patogen S. aureus dan E. coli. Bakteri simbion diisolasi dari fragmen jaringan karang dengan pengenceran bertingkat, kemudian uji aktivitas antibakteri dilakukan dengan menggunakan metode overlay dan difusi paperdisk. Delapan dari 64 isolat aktif menghambat kedua bakteri patogen Staphylococcus aureus dan Escherichia coli. Dua diantaranya merupakan isolat unggul yang menunjukkan aktivitas antibakteri paling baik. Kedua isolat selanjutnya diidentifikasi karakteristik molekular DNA dengan sekuen gen 16S rRNA. Hasil identifikasi 16S rRNA menunjukkan isolat GKP1.4.3 memiliki kesamaan 99% dengan Bacillus pumilus strain NBRC 12092, dan isolat GKP3.2.2 memiliki kesamaan 99% dengan Vibrio natriegens strain NBRC 15636.Porites is a coral which has distributed widely in Indonesia, including Gunungkidul Waters, Yogyakarta. Research has shown that Porites coral symbiont bacteria have antibacterial potency against pathogenic bacteria.This study aims to determine the type of Porites coral symbiont bacteria collected from the waters of Gunungkidul Yogyakarta which has antibacterial activity of S. aureus and E. coli. Bacteria symbionts were isolated from coral tissue fragments by serial dillution method, while antibacterial activity was performed by using overlay and paperdisk diffusion method. Eight of the 64 active isolates inhibited both pathogenic bacteria S. aureus and E. coli. Two of 8 isolates showed stronger antibacterial activity. The two isolates subsequently identified the molecular characteristics of DNA with the 16S rRNA gene sequence. The identification of 16S rRNA showed that GKP1.4.3 isolate had 99% similarity with Bacillus pumilus strain NBRC 12092, and GKP3.2.2 isolate had 99% similarity with Vibrio natriegens strain NBRC 15636.

scholarly journals Antibacterial Activity of Polygonum pulchrum Blume Ethanol Extract on Staphylococcus aureus and Escherichia coli

2018 ◽  
Vol 3 (2) ◽  
pp. 26
Author(s):  
Asman Sadino ◽  
Idin Sahidin ◽  
Wahyuni Wahyuni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Weak Antibacterial Activity

The emergence of resistant bacteria strain has become a global health concern. It encourages the exploration of potential antibacterial agents, particularly from natural sources. The aim of this study was to investigate the antibacterial activity of ethanol extract of root, stems, leaves, and flowers of Polygonum pulchrum Blume against Staphylococcus aureus and Escherichia coli, through disc diffusion method using cup-plate method. Inhibition zone against S. aureus from roots, stems, leaves, and flowers ethanol extract were 3.5 mm, 2.5 mm, 2.25 mm, and 2.62 mm, respectively, while the inhibition zone against E. coli were 2.25 mm, 2.12 mm, 1.62 mm, and 1.75 mm, respectively. In conclusion, ethanol extract of root, stem, leaves, and flower of P. pulchrum Bl possessed weak antibacterial activity against S. aureus and E. coli.Keywords: P. pulchrum Bl, antibacterial, E. coli, S. aureus, cup-plate technique

scholarly journals Antibacterial Activity of Borassus flabellifer Vinegar-Graphene Quantum Dots Against Gram-Positive and Gram-Negative Bacteria

2021 ◽  
Vol 33 (11) ◽  
pp. 2662-2666
Author(s):  
Amnuay Noypha ◽  
Paweena Porrawatkul ◽  
Nongyao Teppaya ◽  
Parintip Rattanaburi ◽  
Saksit Chanthai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Quantum Dots ◽  
Antibacterial Activity ◽  
Graphene Quantum Dots ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli

Borassus flabellifer vinegar–graphene quantum dots (BFV-GQDs) were successfully synthesized using a pyrolysis method with Borassus flabellifer vinegar (BFV) as the precursor. All the samples were characterized using ultraviolet-visible spectrophotometry (UV-Vis), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). The antibacterial activities of BFV-GQDs against strains of Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus) were determined using the agar well diffusion method for preliminary screening, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the broth macro-dilution method. The zones of inhibition were compared with those of citric acid–graphene quantum dots (CA-GQDs). It was observed that the synthesized BFV-GQDs demonstrated excellent antibacterial activity against Staphylococcus aureus (82.3%) and good antibacterial activity against Escherichia coli (73.3%). The MIC of BFV-GQDs against E. coli was 6.25 mg/mL and S. aureus was 12.5 mg/mL, whereas the MBC of BFV-GQDs against E. coli was 12.5 mg/mL and S. aureus was 25.0 mg/mL.

scholarly journals POTENSI ANTIBAKTERI BINTANG LAUT DARI PERAIRAN PANTAI KELURAHAN TONGKAINA MANADO

2019 ◽  
Vol 7 (3) ◽  
pp. 167
Author(s):  
Delpris Piter ◽  
Esther D Angkouw ◽  
Fitje Losung
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Agalactiae ◽  
Antibacterial Agents ◽  
Sea Water ◽  
Diffusion Method ◽  
E Coli ◽  
Linckia Laevigata ◽  
And Staphylococcus Aureus

Starfish is one of the species of the asteroidean class, and is grouped into phylum echinoderms. Echinoderms consist of approximately 6000 species and all live in sea water. Starfish is one source bioactive compounds. Active compounds from starfish have been known to have activities antioxidant, antibacterial, anti-inflammatory, antifungal and immunostimulatory, there are also blue starfish that are potential as antitumor and antibacterial agents. This study aims to obtain crude extracts from several types of starfish, and testing the antibacterial activity of crude starfish extracts against bacteria Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus. Test the antibacterial activity of crude starfish extracs Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata against bacteria E. coli, S. aureus, dan S. agalactiae use the agar diffusion method by means of a well.  L. l extract  can inhibit bacteria e. coli at an extract concentration of 1000 mg/ml with a diameter of inhibitory zone 11.0 mm, whereas at a concentration of 750 mg/ml the test bacteria can still grow on the bacteria S. agalactiae and S. aureus can still grow even though the extract has be given  L. l Keywords: Potential Starfish, Antibacterial Activity, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae Bintang laut merupakan salah satu spesies dari kelas Asteroidea, dan dikelompokkan kedalam filum Echinodermata. Filum Echinodermata  terdiri atas kurang lebih 6000 spesies dan semuanya hidup di air laut. Bintang laut  merupakan salah satu sumber penghasil senyawa bioaktif. Senyawa aktif dari bintang laut telah diketahui memiliki aktivitas antioksidan, antibakteri, antiinflamasi, antifungi dan imunostimulator, ada juga bintang laut biru yang potensial sebagai antitumor dan agen antibakteri. Penelitian ini bertujuan untuk mendapatkan ekstrak kasar dari beberapa jenis bintang laut, dan menguji aktivitas antibakteri ekstrak kasar bintang laut terhadap bakteri Streptococcus agalactiae, Escherichia coli dan  Staphylococcus aureus. Uji aktivitas antibakteri dari ekstrak kasar bintang laut Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata terhadap bakteri E. coli, S. aureus, dan S. agalactiae mengggunakan metode difusi agar dengan cara sumur. Ekstrak L. l mampu menghambat bakteri E.coli pada konsentrasi ekstrak 1.000 mg/ml dengan diameter zona hambat 11.0 mm, sedangkan pada konsentrasi 750 mg/ml bakteri uji masih bisa bertumbuh pada bakteri S. agalactiae dan S. aureus masih dapat bertumbuh walaupun telah diberikan ekstrak L. l.Kata Kunci : Potensi Bintang Laut, Aktivitas Antibakteri, Streptococcus agalactiae, Escherichia coli and  Staphylococcus aureus

scholarly journals UJI ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS TERHADAP Escherichia coli DAN Staphylococcus aureus SERTA POTENSINYA TERHADAP AKTIVITAS ANTI-UV

2019 ◽  
Vol 7 (3) ◽  
pp. 196
Author(s):  
Monika Gabrielle ◽  
Deiske A Sumilat ◽  
Veibe Warouw ◽  
Remy EP Mangindaan ◽  
Chatrien AC Sinjal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Diffusion Method ◽  
Water Fraction ◽  
E Coli ◽  
Self Defense ◽  
Crude Extracts ◽  
Water Fractions

Sponge organisms produce bioactive compounds that are toxic as a means of self-defense. The compound is known to have the potential as an antibacterial and anti-UV which can absorb sunlight with the potential to be used as a material for making sunscreen. The purpose of this study was to obtain antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and anti-UV activity from crude extracts and sponges fractions. The antibacterial test done by agar diffusion method (Kirby and Bauer diffusion disc) and the crude extract and the active fraction of antibacterial compounds were tested in a UV spectrophotometer to see its anti-UV activity. As a result, 4 species of sponges were extracted and partitioned into water fractions, methanol fractions, and n-hexane fractions. All samples were tested for antibacterial activity and the results showed antibacterial activity against S. aureus by Plakortis sp. in crude extract (9 mm) and water fraction (8.6 mm), Agelas sp. in crude extract (7 mm) and in E. coli bacteria shown by Plakortis sp. in crude extract (12.6 mm) and water fraction (9 mm), Liosina sp. in the water fraction (7.6 mm), Haliclona sp. in the water fraction (8 mm) and Agelassp. in crude extract (10.3 mm). Crude extracts and water fractions were tested using a UV spectrophotometer for anti-UV testing, the results showed that crude extract and all the water fractions of four species sponge could absorb UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm).Keywords: sponges, antibacterial, partition, anti-UV

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