scholarly journals Evaluation of the Frequency of Swine Enzootic Pneumonia in one Farm Based on Slaughterhouse, Clinical and Laboratory Monitoring

2021 ◽  
Vol 49 ◽  
Author(s):  
Janine Alves Sarturi ◽  
Carlos Augusto Rigon Rossi ◽  
Rodrigo Dalmina Rech ◽  
Analaura Bianchini Pinheiro ◽  
Kauany Moura Da Silva ◽  
...  

Background: Swine enzootic pneumonia (SEP), caused by the bacterium Mycoplasma hyopneumoniae, is one of the main respiratory diseases of pigs. The purpose of this study was to evaluate the frequency of SEP in one farm with an independent production system, low level of technification and high losses in the meat packing plant, based on slaughterhouse, clinical and laboratory monitoring.Materials, Methods & Results: This study consisted of three monitoring steps, divided into three periods: P1, P2 and P3. In the first step of the study, slaughterhouse reports were analyzed and slaughtering was monitored at one meatpacking plant in Santa Maria, RS, Brazil. The second step of the study started by drawing up a profile of the farm that supplied the pigs to the aforementioned slaughterhouse, after which clinical monitoring was performed on three occasions. The third step of the study (laboratory monitoring) involved collecting lung fragments containing suspected SEP lesions from the meatpacking plant and subjecting them analysis. The average measured enzootic pneumonia (EP) in P3 was 38.54% (P < 0.05) higher than in P1 and 29.79 higher than in P2. The mean frequency of pulmonary emphysema (PE) in P3 was 59.13% (P < 0.05) higher than in P1 and 48.04% higher than in P2. The mean number of lung adhesions (LA) did not differ statistically (P > 0.05) between P1, P2 and P3. As for the mean frequency of pulmonary hepatization (PH), P3 was 48.80% higher (P < 0.05) than P1 and 41.78% higher than P2. With regard to the mean frequency of craniodorsal lung lesions (CLL), P3 was 48.26% higher (P < 0.05) than P1 and 40.77% higher than P2. The mean frequency of disseminated lesions (DL) and the pneumonia severity index (PSI) showed no statistical difference (P > 0.05) between the three evaluated periods. On the other hand, in the finishing period (FP), the frequency of coughing and sneezing was 37.56% (P < 0.05) higher in P1 than in P3. The frequency of coughing and sneezing evaluated in the nursery phase (CF) did not differ (P > 0.05) between periods. The samples sent to the laboratory MicroVet tested positive for M. hyopneumoniae, Influenza virus and Pasteurella multocida capsular type A. In the samples sent to the laboratory LABAC, the mean PCRmo (PCR for identification of mollicutes) did not differ (P > 0.05) in the analyzed periods, although the presence of DNA from bacteria of the class Mollicutes was confirmed in the fragments. In addition, the mean results of PCRmy (PCR for identification of M. hyopneumoniae) also did not differ (P > 0.05), although they revealed positivity for M. hyopneumoniae. Discussion: In relationship to the mean frequency of PE in each analyzed period, our study revealed an increase in the number of pulmonary lesions at slaughter during the periods under analysis. This finding is in agreement with those of other authors, who explain that pneumonias is one of the main health problems for swine producers. Some lesions are identified more frequently in routine inspections in meatpacking plants, including pulmonary emphysema, lung adhesions and pneumonia caused by M. hyopneumoniae. In our study, we found that the clinical signs of respiratory distress decline between the periods under evaluation, but during slaughter, the lesions suggestive of SEP continued to show an upward trend, mainly because they were chronic. Other authors state that several control measures should be adopted to ensure good zootechnical indices, including vaccination, strategic medication and the reduction of risk factors, thereby minimizing the level of M. hyopneumoniae infection in the herd.

2020 ◽  
Vol 57 (4) ◽  
Author(s):  
Wafaa Abd El-Ghany ◽  
Hanan Ali Ahmed ◽  
Ali Zaher Qandoos ◽  
Mohamed Abd El-Rahman Bosila Bosila

This study was carried out to evaluate the efficacy of vaccination of layer chickens with inactivated FC bacterin prepared from local Egyptian strains of Pasteurella multocida (P. multocida). A total of 200 layer chickens were divided into 5 equal groups, 40 for each. At the age of 6 weeks, chickens in groups (A) and (B) were vaccinated with P. multocida serotypes A:1 and A:3, respectively, booster doses were given after 3 weeks (9 weeks old) and challenge was done with virulent serotypes A:1 and A:3 at 2 weeks later (11 weeks old). Chickens in groups (C) and (D) were not vaccinated, only challenged with P. multocida serotype A:1 and A:3, respectively. Birds in group (E) were kept as non-vaccinated and non-challenged. Blood samples were collected weekly from all groups for humoral immune response. All the birds were kept under observation for signs, mortalities, lesions and re-isolation of challenging organism and for histopathological examination. Results of the mean Enzyme Linked Immuno-Sorbent Assay (ELISA) revealed that the highest level was at 5 weeks post vaccination as the titers reached to 3970 in group (A) and 3905 in group (B). The clinical signs, mortality rate and lesions were mild in the vaccinated birds while severe lesions were in non-vaccinated and challenged birds. The protection rates were 85 % and 80 % in groups (A) and (B); respectively, while 10 % and 20 % in groups (C) and (D); respectively. The re-isolation rates of P. multocida after challenge were 95 % and 90 % in non-vaccinated-challenged birds with P. multocida serotypes A:1 and A:3; respectively, while they were 25 % and 15 % in vaccinated-challenged groups with P. multocida serotypes A:1 and A:3; respectively. Histopathological examination of P. multocida vaccinated-challenged birds revealed mild to no microscopic lesions when compared with non-vaccinated challenged chickens. In conclusion, the prepared FC inactivated bacterin from the local Egyptian predominant P. multocida serovars proved efficacy and protection of layer chickens. Key words: Pasteurella multocida; chickens; immunization; protection; Egypt UČINKOVITOST CEPLJENJA KOKOŠI NESNIC Z INAKTIVIRANO BAKTERIJO KOLERE PERJADI, PRIPRAVLJENE IZ LOKALNIH EGIPTOVSKIH SEVOV BAKTERIJE Pasteurella multocida Povzetek: Raziskava je bila izvedena z namenom ocenitve učinkovitosti cepljenja kokoši nesnic z inaktivirano bakterijo FC, pripravljeno iz lokalnih egiptovskih sevov bakterije Pasteurella multocida (P. multocida). Skupno 200 kokoši nesnic je bilo razdeljenih v 5 enakih skupin. V vsaki skupini je bilo 40 kokoši. Pri 6 tednih smo kokoši v skupinah A in B cepili s serotipoma P. multocida A:1 in A:3, po 3 tednih, ko so bile živali stare 9 tednov, so dobile poživitvene doze cepiva. Po dveh tednih (v starosti 11 tednov) so bile kokoši okužene z virulentnima serotipoma A:1 in A:3. Piščanci v skupinah C in D niso bili cepljeni temveč samo okuženi s serotipoma A:1 in A:3. Kokoši v skupini E niso bile niti cepljene, niti okužene. Vzorci krvi so bili odvzeti pri vseh skupinah tedensko za preverjanje humoralnega imunskega odziva. Vse kokoši smo stalno opazovali in beležili prisotnost bolezenskih znakov, različnih ran in umiranje kokoši. Pri poginulih kokoših smo osamili bakterije ter opravili histopatološki pregled. Rezultati encimsko-imunskega testa (ELISA) so pokazali da je bila najvišja stopnja zaščite dosežena 5 tednov po cepljenju, saj so titri dosegli 3970 v skupini A in 3905 v skupini B. Klinični znaki, stopnja umrljivosti in rane so bili pri cepljenih kokoših blagi, hude rane pa so bile vidne pri necepljenih in okuženih kokoših. Stopnja zaščite je bila v skupinah A in B 85- oziroma 80-odstotna, v skupinah C in D pa 10- oziroma 20-odstotna. Stopnje ponovne izolacije P. multocida po okužbi so bile 90 in 95 odstotkov pri kokoših, ki niso bile cepljene, medtem, ko so bile v skupinah, ki so bile okužene s P. multocida serotipa A:1 in A:3 15- in 25-odstotkov. Histopatološki pregled cepljenih in okuženih kokoši je pokazal popolno odsotnost ali prisotnost blagih mikroskopskih poškodb, medtem ko so imele necepljene okužene kokoši bolj obsežne histopatološke poškodbe. Pripravljena inaktivirana bakterija FC iz lokalnih egiptovskih prevladujočih serovarov P. multocide se je izkazala za učinkovito zaščito kokoši nesnic.Ključne besede: Pasteurella multocida; kokoši; imunizacija; zaščita; Egipt


1971 ◽  
Vol 69 (4) ◽  
pp. 717-725
Author(s):  
L. H. Collier ◽  
Elaine Lightman

SUMMARYTrachoma vaccines are usually assayed by testing their ability to protect monkeys or baboons against subsequent challenge of the conjunctiva with a pathogenic strain of trachoma/inclusion conjunctivitis (TRIC) agent. In such experiments the course of infection in vaccinated baboons was compared in terms of arbitrary scores assigned to a range of clinical signs, and of counts of TRIC inclusions in conjunctival scrapings. Analysis of many such scores indicated that after a large challenge dose of strain MRC-4s, the scores for signs of inflammation reached their maximum earlier than the follicle score; the inflammation score was closely related to the number of inclusions, whereas the follicle score was not. With this system, the optimum periods for eliciting differences between vaccinated and control measures varied according to the sign used; it was later for follicles than for inflammation or inclusions. For assessing the influence of vaccination, the mean of the inflammation scores read weekly for the first 3 weeks after challenge and the mean inclusion score over the same period were equally satisfactory, and either was rather better than the mean of three follicle scores taken over the period 3–6 weeks.For assessing the influence of vaccines or therapeutic agents on experimental trachoma it is important to determine which signs discriminate best between treated and control animals, and the optimum times for measuring them.


2021 ◽  
Vol 3 (2) ◽  
pp. 42-49
Author(s):  
Wakgari Abirham Haile ◽  

This seminar review is aimed to provide information about the IBK which results in ocular pain and loss of vision that would result in the reduction of the market price of the affected animal. Infectious Bovine Keratoconjuctivitis (IBK) or ‘pink eye’ is a common and highly contagious ocular disease. The disease is caused by the bacteria family Moraxellaceae, genus Moraxella and species Moraxella bovis. M. bovis is a gram-negative rod. The occurrence and distribution of the disease are worldwide and the persistence of the disease from year to year is by means of infected animals, which can act as carriers. Transmission is unusual in the absence of flies and occurs generally in their presence. A number of factors such as tall grass, weeds, dust, face flies and ultraviolet radiation, and other stress factors contribute to the disease occurrence. The pathogenesis of IBK is likely associated with collagenase release from epithelial cells, fibroblasts, and neutrophils. Hydrolytic enzymes of M. bovis possess the ability to degrade lipids, mucopolysaccharides, and matrix proteins, which may contribute to corneal ulceration. The first signs of pinkeye are characterized by excessive tearing, blinking, photophobia, and swelling of the eyelids and conjunctiva. As the disease progresses, the ocular discharge becomes purulent. The disease is usually diagnosed with clinical signs like excessive lacrimation and culturing of the bacteria from ocular exudates. IBK is differentially diagnosed from M. bovis, Pasteurella multocida, IBRT, and Thelaziasis. Drugs may be delivered to the eye in several ways: subconjunctival injection, topical application, and systemic administration to treat the diseases. Vaccination and fly control are some of the prevention and control measures. This disease is economically very important which causes severe ocular disorder in cattle which may result in the suffering of the animal from pain and loss of vision thereby economic loss due to bodyweight loss by the stress from pain, inability to feed properly, and the blindness that reduces the price of sale. Therefore, it is recommended that the susceptible cattle should be housed to avoid exposure to UV radiation and the populations of face flies should be controlled to minimize the incidence and transmission of IBK.


Author(s):  
P. R. Chavelikar ◽  
G. and Neha Rao C. Mandali ◽  
Neha Rao

Ruminal acidosis is an important clinical emergency in small ruminants. In this study, eight healthy farm goats and 24 goats presented at TVCC of the college of Veterinary Sciences and A.H., Anand with clinical signs of ruminal acidosis and having rumen liquor pH below 6 were examined for alterations in the ruminal fluid and serum biochemical parameters. Among various rumen fluid parameters evaluated, the mean values of rumen fluid pH decreased significantly (4.71±0.11 vs. 6.90±0.10), while sediment activity time (46.67±1.20 vs. 24.50±0.78 min) and methylene blue reduction time (29.50±0.73 vs. 10.03±0.27 min) increased significantly in acidotic goats. The normal greenish, aromatic viscous color, odour and consistency of rumen fluid of healthy goats also changed to milky grey/creamy, sour/pungent watery in acidotic goats. The rumen protozoal activity decreased to nil in acidotic goats as compared to the healthy goats. Among various serum biochemical constituents, the mean values of glucose (92.43±1.37 vs. 74.13±1.83 mg/dl), BUN (26.49±0.47 vs. 22.63±1.19 mg/dl), serum creatinine (01.01±0.02 vs. 00.83±0.02 mg/dl) and albumin (03.22±0.03 vs. 03.05±0.05 g/dl), ALT (56.75±1.55 vs. 27.88±1.14 IU/L) and AST (93.25±1.82 vs. 54.00±1.75 IU/L), increased significantly, while there was significant decrease in serum calcium (09.09±0.14 vs. 10.29±0.08 mg/dl) in acidotic goats. The mean values of alkaline phosphatase (IU/L) in acidotic goats increased non-significantly from the base values of healthy goats.


Author(s):  
P. R. Chavelikar ◽  
G. C. Mandali ◽  
D. M. Patel

Ruminal acidosis is one of the most important clinical emergencies in sheep and goats resulting into high mortality rate. In the present study, eight healthy farm goats and 24 goats presented to the TVCC of the college with clinical signs of ruminal acidosis like anorexia, tympany, increased pulse and respiratory rate, reduced body temperature, doughy rumen, enteritis, oliguria, grinding of teeth, purulent nasal discharge, muscle twitching, arched back, dehydration and recumbency with rumen liquor pH below 6 were examined for haematological alterations using autohaematoanalyzer. Among various haematological parameters evaluated from acidotic goats, the mean values of Hb (12.21±0.17 vs. 10.86±0.15 g/dl), TEC (14.28±0.16 vs. 12.04±0.36 ×106/ μl), TLC (13.43±0.11 vs. 11.11±0.27 ×103/μl), PCV (36.91±0.53 vs. 29.88±0.55%), neutrophils (64.54±0.93 vs. 28.13±0.92%), MCV (23.38±0.37 vs. 19.38±1.34 fl) and MCH (7.03±0.08 vs. 6.31±0.25 pg) were found significantly increased, while the mean values of lymphocytes (28.00±0.82 vs. 65.38±0.80%) and MCHC (24.55 ±0.26 vs. 34.88±0.97 g/dl) were decreased significantly from the base values of healthy goats. It was concluded that ruminal acidosis induced due to accidental heavy ingestion of readily fermentable carbohydrate rich grains and food waste significantly altered the haematological profile concurrent with clinical manifestations in goats, and hence can be used to assess the severity of the disease.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Clare Shakespeare ◽  
Handsome Dube ◽  
Sikhangezile Moyo ◽  
Solwayo Ngwenya

Abstract Background On the 27th of March 2020 the Zimbabwean government declared the Covid-19 pandemic a ‘national disaster’. Travel restrictions and emergency regulations have had significant impacts on maternity services, including resource stock-outs, and closure of antenatal clinics during the lockdown period. Estimates of the indirect impact of Covid-19 on maternal and perinatal mortality was expected it to be considerable, but little data was yet available. This study aimed to examine the impact of Covid-19 and lockdown control measures on non-Covid outcomes in a government tertiary level maternity unit in Bulawayo, Zimbabwe, by comparing maternal and perinatal morbidity and mortality before, and after the lockdown was implemented. Methods This was a retrospective, observational study, using a cross-sectional design to compare routine monthly maternal and perinatal statistics three months before and after Covid-19 emergency measures were implemented at Mpilo Central Hospital. Results Between January-March and April-June 2020, the mean monthly deliveries reduced from 747.3 (SD ± 61.3) in the first quarter of 2020 to 681.0 (SD ± 17.6) during lockdown, but this was not statistically significant, p = 0.20. The Caesarean section rates fell from a mean of 29.8% (SD ± 1.7) versus 28.0% (SD ± 1.7), which was also not statistically significant, p = 0.18. During lockdown, the percentage of women delivering at Mpilo Central Hospital who were booked at the hospital fell from a mean of 41.6% (SD ± 1.1) to 35.8% (SD ± 4.3) which was statistically significant, p = 0.03. There was no significant change, however, in maternal mortality or severe maternal morbidity (such as post-partum haemorrhage (PPH), uterine rupture, and severe preeclampsia/eclampsia), stillbirth rate or special care baby unit admission. There was an increase in the mean total number of early neonatal deaths (ENND) (mean 18.7 (SD ± 2.9) versus 24.0 (SD ± 4.6), but this was not statistically significant, p = 0.32. Conclusions Overall, maternity services at Mpilo showed resilience during the lockdown period, with no significant change in maternal and perinatal adverse outcomes, with the same number of man-hours worked before and during the lockdown Maternal and perinatal outcomes should continue to be monitored to assess the impact of Covid-19 and the lockdown measures as the pandemic in Zimbabwe unfolds. Further studies would be beneficial to explore women’s experiences and understand how bookings and deliveries at local clinics changed during this time.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Larissa Pessidjo Djomatcho ◽  
Mathurin Pierre Kowo ◽  
Antonin Ndjitoyap Ndam ◽  
Sylvain Raoul Simeni Njonnou ◽  
Gabin Ulrich Kenfack ◽  
...  

Abstract Background Minimal hepatic encephalopathy (MHE) is the presence of neuropsychological abnormalities detectable by psychometric tests. Psychometric Hepatic Encephalopathy Score (PHES) is a gold standard test for the early diagnosis of MHE in cirrhotic patients. The aim of this study was to standardize the PHES in a healthy Cameroonian population and to evaluate the prevalence of MHE among cirrhotic patients. Methods This was a prospective, multicentric study from 1 December 2018 to 31 July 2019 in two groups: healthy volunteers and cirrhotic patients without clinical signs of hepatic encephalopathy. The results of the number connection test-A, number connection test-B, serial dotting test, line tracing test were expressed in seconds and those of the digit symbol test in points. Results A total of 102 healthy volunteers (54 men, 48 women) and 50 cirrhotic patients (29 men, 31 women) were included. The mean age was 38.1 ± 12.55 years in healthy volunteers and 49.3 ± 15.6 years in cirrhotic patients. The mean years of education level was 11.63 ± 4.20 years in healthy volunteers and 9.62 ± 3.9 years in cirrhotic patients. The PHES of the healthy volunteer group was − 0.08 ± 1.28 and the cut-off between normal and pathological values was set at − 3 points. PHES of the cirrhotic patients was − 7.66 ± 5.62 points and significantly lower than that of volunteers (p < 0.001). Prevalence of MHE was 74% among cirrhotic patients. Age and education level were associated with MHE. Conclusion PHES cut-off value in Cameroonians is − 3, with MHE prevalence of 74% among cirrhotic patients.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rine Christopher Reuben ◽  
Shovon Lal Sarkar ◽  
Habiba Ibnat ◽  
Md. Ali Ahasan Setu ◽  
Pravas Chandra Roy ◽  
...  

AbstractPasteurella multocida causes fowl cholera, a highly contagious poultry disease of global concern, causing significant ecological and economic challenges to the poultry industry each year. This study evaluated the effects of novel multi-strain probiotics consisting of Lactobacillus plantarum, L. fermentum, Pediococcus acidilactici, Enterococcus faecium and Saccharomyces cerevisiae on growth performance, intestinal microbiota, haemato-biochemical parameters and anti-inflammatory properties on broilers experimentally challenged with P. multocida. A total of 120 birds were fed with a basal diet supplemented with probiotics (108 CFU/kg) and then orally challenged with 108 CFU/mL of P. multocida. Probiotics supplementation significantly (P < 0.05) improved growth performance and feed efficiency as well as reducing (P < 0.05) the population of intestinal P. multocida, enterobacteria, and mortality. Haemato-biochemical parameters including total cholesterol, white blood cells (WBC), proteins, glucose, packed cell volume (PCV) and lymphocytes improved (P < 0.05) among probiotic fed birds when compared with the controls. Transcriptional profiles of anti-inflammatory genes including hypoxia inducible factor 1 alpha (HIF1A), tumor necrosis factor- (TNF) stimulated gene-6 (TSG-6) and prostaglandin E receptor 2 (PTGER2) in the intestinal mucosa were upregulated (P < 0.05) in probiotics fed birds. The dietary inclusion of the novel multi-strain probiotics improves growth performance, feed efficiency and intestinal health while attenuating inflammatory reaction, clinical signs and mortality associated with P. multocida infection in broilers.


1986 ◽  
Vol 41 (1-2) ◽  
pp. 215-224 ◽  
Author(s):  
Winfried Fichtner ◽  
Axel Markworth ◽  
Norbert Weiden ◽  
Alarich Weiss

The temperature dependence of salts M(1)H(Cl3CCOO)2 and molecular compounds of trichloroacetic acid with amines and benzaldehydes, TCA · X, was studied,The data fit rather well to the known dependence of the mean frequency shift Δ <v(35Cl)> on the pkadifference of X with respect to TCA. A linear relation is observed between the bleaching out temperature Tb of the 35Cl NQR lines and Δ <v(35Cl)> for M(1)H(Cl3CCOO)2 and for TCA · X, X = benzaldehydes.


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