scholarly journals PRODUKSI ENZIM LIPASE DARI Aspergillus niger ISOLAT KAPANG KOPRA DENGAN MENGGUNAKAN MEDIUM KELAPA PARUT

KOVALEN ◽  
2017 ◽  
Vol 3 (3) ◽  
pp. 269
Author(s):  
Indah Indah ◽  
Mappiratu Mappiratu ◽  
Musafira Musafira
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Water Content ◽  
High Activity ◽  
Incubation Time ◽  
Enzyme Production ◽  
Block Design ◽  
Lipase Enzyme ◽  
Randomized Block Design

The investigation about the lipase enzyme production from Aspergillus niger using grated coconut as a medium has been done. The aim of the study is to determine the best incubation time, pH and water content which produced lipase enzyme with high activity. Randomized Block Design (RBD) with 2 factorial pattern (incubation time and pH of the medium) was used in this research. Each factor consists of three levels and it was done in the triple. The level of incubation time and of pH were 48, 60, 72 hours and pH 5, 6, and 7, respectively. The observed parameter was the obtained lipase enzyme activity. The result showed that the highest activity was achieved at pH 7 for 48 hours of incubation time with 45% of water content. The amount of lipase enzyme activity was 1.70 µmol/ml.minutes.Keywords: mold coconut, Aspergillus niger, lipase

scholarly journals Fermentasi Kelapa Parut Bebas Protein dengan Aspergillus niger untuk Menghasilkan Lipase

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 45-52
Author(s):  
Rifka Aulia ◽  
Syaiful Bahri ◽  
Meryany Ananda
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Water Content ◽  
Incubation Time ◽  
Fermentation Medium ◽  
Enzyme Extract ◽  
Enzyme Protein ◽  
Lipase Enzyme ◽  
Randomized Design ◽  
Completely Randomized Design

The research about the fermentation of protein-free grated coconut with Aspergillus niger to produce of crude lipase enzyme has been carried out. This study aims to determine the effect of water content on fermentation medium and the incubation time which results lipase enzyme extract with the highest activity. This study used a Completely Randomized Design (CRD) consisting of 2 factors, i.e. variations of water content in medium of 40%, 50%, 60%, and 70% and incubation times of 48, 96, 144, and 192 hours. The parameters observed were lipase enzyme activity produced in each treatment. The results showed that the highest medium water content was 70% with the lipase enzyme activity 4.33 µmol/mL.minute and the best incubation time was 96 hours with the lipase enzyme activity of 0.83 µmol/mL.minute. Keywords: Lipase enzyme, protein-free grated coconut, Aspergillus niger

Stem Cold Hardiness, Leaf Heat Tolerance, Growth, and Performance of Six Japanese Maples (Acer palmatum) in Northern Illinois

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 512e-512
Author(s):  
A.M. Shirazi
Keyword(s):  
Water Content ◽  
Heat Tolerance ◽  
Cold Hardiness ◽  
Block Design ◽  
Leaf Tissue ◽  
Ion Leakage ◽  
Randomized Block Design ◽  
Leaf Tissues ◽  
Artificial Freezing ◽  
And Performance

Six different Japanese Maples (Acer palmatum) cultivars `Water Fall', `Burgundy Lace', `Crimson Queen', `Oshio-Beni', `SangoKaKu', and `Bloodgood' from Monrovia Nursery were planted in a randomized block design on 4 June 1997 at the The Morton Arboretum. Leaf heat tolerance was evaluated by measuring ion leakage of the leaf tissue at 25–60 °C in July, Aug., and Sept. 1997. The LT50 (the temperature at which 50% of the tissues were injured) of all the cultivars were higher in July (≈53 °C) and were lower in September (≈47 °C). Water content of the leaf tissues were higher in July compare to August and September and were not related to heat tolerance of most cultivars. Stem cold hardiness was performed by artificial freezing tests in Oct., Dec., and Feb. 1997/98. The Lowest Survival Temperature (LST) for the most hardy to least hardy cultivars in October and December were: `Burgundy Lace' (–15, –27 °C), `Bloodgood' (–18, –24 °C), `Oshio-Beni' (–15, –24 °C), `Crimson Queen' (–15, –18 °C), `Water Fall' (–9, –18 °C) and `SangoKaKu' (–9, –12 °C), respectively. Growth, dormancy development, spring budbreak and performance of these cultivars will be compared.

PERANAN TRICHOKOMPOS DAN PUPUK KCL DALAM MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN BAWANG MERAH DI TANAH BERPASIR (The Role of Trichokompos and KCL Fertilizer to Control Fusarium Will Disease on Onion in Sandy Soil)

AgriPeat ◽  
2019 ◽  
Vol 20 (01) ◽  
pp. 19-26
Author(s):  
Admin Journal
Keyword(s):  
Research Design ◽  
Fusarium Wilt ◽  
Sandy Soil ◽  
Incubation Time ◽  
Block Design ◽  
Wilt Disease ◽  
Single Factor ◽  
Fusarium Wilt Disease ◽  
Randomized Block Design

ABSTRACTThe role of Trichocompost and KCl fertilizer to control Fusarium wilt disease on onion in sandy soil. Fusarium wilt on onion is an interesting disease it is can loss the onion yield. The purpose of research to study trichocompost and KCl fertilizer role to control Fusarium wilt disease on ann onion. The research design used a Factorial Randomized Block Design with 2 factors. The first factor is 4 levels trichocompost, it is: without trichocompost (T0), trichocompost 10 t.ha-1 dosage (T1), trichocompost 20 t.ha-1 dosage (T2), trichocompost 30 t.ha-1 dosage (T3). The second factor is 3 levels KCl fertilizer, it is: without KCl (K0), KCl 100 kg.ha-1 dosage (K1), KCl 200 kg.ha-1 dosage (K2). Result of this research showed the application of trichocompost 10 t.ha-1 dosage and KCl 100 kg.ha-1 dosage can inhibit Fusarium wilt incubation time, can inhibit the patogen development with effective value 89,23%, the single factor it is aplication trichocompost 10 t.ha-1 dosage and trichocompost 30 t.ha-1 dosage not significant to dried onion bulb weight per clump of onion plant.Key words: Trichocompost, KCl fertilizer, Fusarium wilt disease, onion, sandy soil.ABSTRAKPenyakit layu Fusarium merupakan salah satu penyakit penting dapat menurunkan produksi bawang merah hingga 50%. Tujuan penelitian untuk mengetahui peranan trichokompos dan pupuk KCl dalam mengendalikan penyakit layu fusarium pada tanaman bawang merah. Penelitian menggunakan Rancangan Acak Kelompok faktorial dua faktor perlakuan. Faktor pertama 4 taraf dosis trichokompos yaitu: tanpa trichokompos (T0), trichokompos dosis 10 t.ha-1 (T1), trichokompos dosis 20 t.ha-1 (T2), trichokompos dosis 30 t.ha-1 (T3). Faktor kedua 3 taraf dosis pupuk KCl yaitu: tanpa pupuk KCl (K0), pupuk KCl dosis 100 KCl kg.ha-1 (K1), pupuk KCl dosis 200 KCl kg.ha-1 (K2). Hasil penelitian menunjukkan pemberian trichokompos 10 t.ha-1 dan pupuk KCl 100 kg.ha-1 dapat memperpanjang masa inkubasi penyakit, menekan serangan penyakit layu Fusarium dengan nilai efektivitas sangat baik (89,23%), perlakuan tunggal trichokompos dosis 10 t.ha-1 tidak berbeda nyata dengan dosis 30 t.ha-1 terhadap bobot umbi kering per rumpun tanaman bawang merah.Kata kunci: penyakit layu Fusarium, pupuk KCl, tanah berpasir, tanaman bawang merah, trichokompos.

scholarly journals Pengaruh Konsentrasi Pelarut dan Lama Pengadukan Terhadap Karakteristik Glukomanan Ubi Talas sebagai Bahan Edible Film Buah Segar

2019 ◽  
Vol 4 (2) ◽  
pp. 62
Author(s):  
Amna Hartiati ◽  
I. A. Mahatma Tuningrat
Keyword(s):  
Water Content ◽  
Sweet Potato ◽  
Starch Content ◽  
Block Design ◽  
Edible Film ◽  
Potato Flour ◽  
Solvent Concentration ◽  
Randomized Block Design ◽  
Stirring Time ◽  
Sweet Potato Flour

Tujuan umum yang ingin dicapai dari penelitian ini adalah 1) untuk mengetahui pengaruh konsentrasi pelarut dan waktu pengadukan pada karakteristik glukomanan tepung ubi talas yang diproduksi, 2) untuk mendapatkan glukomanan dengan karakteristik terbaik dari tepung  ubi  talas. Pelarut yang digunakan adalah etanol pada konsentrasi  50, 60 dan 70% dengan waktu pengadukan 30, 60 dan 90 menit. Penelitian ini menggunakan rancangan acak kelompok faktorial dengan dua faktor: konsentrasi pelarut dan waktu pengadukan selama ekstraksi tepung ubi talas. Konsentrasi pelarut yang digunakan untuk ekstraksi glukomanan terdiri dari 3 level, yaitu 50, 60 dan 70% (v / v). Waktu pengadukan selama ekstraksi terdiri dari tiga level, yaitu 30, 60 dan 90 menit. Parameter yang diamati adalah rendemen, kadar glukomanan, kadar pati dan kadar air. Penelitian ini terdiri dari 2 (dua) tahap, yaitu pembuatan glukomanan dari tepung ubi talas dan tahap kedua adalah hasil terbaik pada tahap satu yang akan digunakan sebagai bahan  edible  film buah segar. Hasil penelitian menunjukkan bahwa konsentrasi pelarut dan waktu pengadukan memiliki pengaruh yang sangat signifikan terhadap kadar glukomanan, kadar air dan interaksinya, tidak berpengaruh nyata terhadap kadar pati, rendemen glukomanan. Karakteristik terbaik glukomanan adalah perlakuan konsentrasi pelarut etanol 70% dan waktu pengadukan 90 menit dengan kadar glukomanan 60,166%; kadar pati 0,072%; kadar air 7,313% dan rendemen  9,320% untuk tepung talas.   The general objectives to be achieved from this study were: 1) to determine the effect of solvent concentration and stirring duration on the glucomannan character of Taro sweet potato flour produced; 2) to obtain glucomannan with the best characteristics of Taro sweet potato flour. The solvent used is ethanol at a concentration of 50, 60 and 70% with a stirring time of 30, 60 and 90 minutes. The study used a factorial randomized block design with two factors: solvent concentration and stirring time during the extraction of Taro sweet potato flour. The concentration of solvent used for glucomannan extraction consisted of 3 levels, namely 50, 60 and 70% (v / v). The duration of stirring during extraction consists of three levels, namely 30, 60 and 90 minutes. The parameters observed were the yield, glucomannan content, starch content, and water content. This study consisted of 2 (two) stages, namely the manufacture of glucomannan from Taro sweet potato flour and the second stage was the best result in stage one would be used as an edible film chart on fresh fruit. The results showed that solvent concentration and stirring time had a very significant effect on glucomannan content, water content, and interaction and did not significantly affect starch content, the yield of glucomannan Taro flour. The best characteristics of glucomannan were the treatment of 70% ethanol solvent concentration and stirring time of 90 minutes with glucomannan content of 60.116%; 0.072% starch content; moisture content 7.313% and 9.320% yield for Taro yam flour.  

scholarly journals SUBSTITUSI TEPUNG ALMOND DENGAN TEPUNG KEMIRI (Aleurites moluccana Wild) TERHADAP KARAKTERISTIK KULIT MACARON

2019 ◽  
Vol 8 (2) ◽  
pp. 122
Author(s):  
Ida Ayu Ganitri Adnyasuari ◽  
I Gusti Ayu Ekawati ◽  
Ni Made Indri Hapsari Arihantana
Keyword(s):  
Protein Content ◽  
Water Content ◽  
Analysis Of Variance ◽  
Block Design ◽  
Fat Content ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Randomized Block Design ◽  
The Right ◽  
Aleurites Moluccana

This research aims to identify the substitution of almond flour with candlenut flour on the characteristics of macaron shell, and to identify the right concentration of almond flour with candlenut flour on the characteristics of macaron shell. This research used randomized block design with the treatment ratio of almond flour with candlenut flour, which consists of 6 levels such as: 100% : 0%, 80% : 20%, 60% : 40%, 40% : 60%, 20% : 80%, and 0% : 100%. Each treatment was repeated 3 times, resulting in 18 experimental units. The data were then analyzed using Analysis of Variance and if the treatment had an effect on the parameter, the Duncan test was performed. The result showed that substitution of almond flour with candlenut flour had a significant effect on water content, ash content, protein content, fat content, carbohydrate content, swelling, flavor (hedonic), texture (hedonic), taste (scoring) and overall acceptance (hedonic). Substitution of almond flour with candlenut flour 40% had the best characteristics, with 2,40% water content, 2,51% ash content, 9,40% protein content, 18,98% fat content, 66,70% carbohydrate content, 130,81% swelling, color, flavor, taste, and overall acceptance liked, taste candlenut medium and texture very soft.

scholarly journals PENGARUH KONSENTRASI NATRIUM METABISULFIT DAN LAMA PERENDAMAN TERHADAP KARAKTERISTIK BUBUK BUNGA KENIKIR (Tagetes erecta L.)

2019 ◽  
Vol 7 (1) ◽  
pp. 81
Author(s):  
Komang Adi Darmawan ◽  
Ni Made Wartini ◽  
Luh Putu Wrasiati
Keyword(s):  
Water Content ◽  
Block Design ◽  
Tagetes Erecta ◽  
Sodium Metabisulfite ◽  
Total Carotenoid ◽  
Soaking Time ◽  
Brightness Level ◽  
Randomized Block Design ◽  
Residue Levels ◽  
Tagetes Erecta L

Marigold powder can be used as natural orange dyes extract of marigold powder contain ?-carotene. The aim of this research was to investigate the effect of sodium metabisulfite (Na2S2O5) concentration and soaking time on the characteristics of marigold flower powder and determine the best treatment of the concentration of sodium metabisulphite (Na2S2O5) and soaking time to produce powdered marigold flowers. This research used factorial randomized block design with 2 factor experiments. The first factor is the concentration of sodium metabisulfite (Na2S2O5) which consists of 3 levels: 2000, 2500, and 3000 ppm. The second factor is the soaking time which consists of 3 levels: 10, 20, and 30 minutes. The results of research showed that the treatment of sodium metabisulfite (Na2S2O5) concentration take effect on water content, total carotenoid levels, brightness (L*), yellowish level (b*), and sulfite residue levels, but no effect to the level of redness (a*). The treatment of soaking time take effect on the levels of sulfite residues but no effect on water content, total carotenoid levels, brightness level (L*), yellowish level (b*), and redness level (a*). Soaking treatment in sodium metabisulfite (Na2S2O5) solution with a concentration of 2500 ppm and soaking time of 10 minutes, is the best treatment to produce marigold flower powder with the characteristics of water content 11,40%, total carotenoid levels of 18.91%, brightness level (L*) 44.19, redness level (a*) 63.80, yellowish level (b*) 56.86, and sulfite residue levels of 182.40 ppm. Keywords : Tagetes erecta L, sodium metabisulfite, carotenoid, color.

scholarly journals EVALUASI SIFAT KIMIA DAN SENSORI PERMEN JELLY JAMUR TIRAM PUTIH PADA BERBAGAI KONSENTRASI GELATIN

2021 ◽  
Vol 11 (1) ◽  
pp. 43-53
Author(s):  
Sussi Astuti ◽  
Dwi Ardiansyah ◽  
Susilawati Susilawati
Keyword(s):  
Data Analysis ◽  
Water Content ◽  
Block Design ◽  
Ash Content ◽  
Oyster Mushroom ◽  
Single Factor ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Gelatin Concentration ◽  
Sugar Levels

The purpose of the research to determine the concentration of gelatin that produce white oyster mushroom jelly candy which appropriate with the chemical and sensory characteristic of standard ISO 3547.2-2008.  The research arranged in a Complete Randomized Block Design (CRBD) by a single factor that is gelatin concertration on six grade of 5%, 10%, 15%, 20%, 25%, and 30% with four repetition.  Data analysis of variance and a further test with the Least Significant Difference (LSD) at 5% level.  The results showed that the best treatment was found in 20% of gelatin concentration that produces flavor white oyster mushroom jelly candy with score of 2.98 (rather typical white oyster mushroom), elasticity with a score of 3.89 (chewy), color with score of 3.71 (like), the overall acceptance with score of 3.83 (like), the water content of 18.27% (bb), ash content of 0.25% (bb), reduced sugar levels of 0.28% (bb), and sucrose levels of 51.33% (bb) which appropriate with SNI jelly candy 3547.2-2008.

scholarly journals Optimasi Waktu Inkubasi Lactobacillus rhamnosus SKG 34 Dalam Produksi Enzim Penggumpal Susu

2020 ◽  
Vol 9 (2) ◽  
pp. 108
Author(s):  
Wulandari Setiadarma ◽  
Dewa Gede Mayun Permana ◽  
Komang Ayu Nocianitri
Keyword(s):  
Incubation Time ◽  
Protease Activity ◽  
Block Design ◽  
Lactobacillus Rhamnosus ◽  
Duncan Multiple Range Test ◽  
Milk Clotting ◽  
Randomized Block Design ◽  
Milk Clotting Enzyme ◽  
Specific Protease ◽  
Range Test

This study aims to determine the effect of incubation time on milk clotting enzyme (MCE) activity produced by L. rhamnosus SKG 34 and determine the optimum incubation time to produce the highest its activity. This study used a randomized block design (RBD) with incubation time as a treatment consisting of 8 levels, that were 6 hours, 12 hours, 18 hours, 24 hours, 30 hours, 36 hours, 42 hours, and 48 hours. Data were analyzed with Variance Analysis (ANOVA) than followed by Duncan Multiple Range Test (DMRT). The analyzed were repeated 3 times resulting in 24 experimental units. The results showed that the incubation time significantly affected protease activity, MCE activity, specific protease activity and ratio of MCE to protease but did not affect the total LAB. The optimum incubation time of L. rhamnosus SKG 34 is 12 hours with total LAB 1.83 x 109 CFU/ml and protease activity 180.67 U/ml, MCE activity 595.06 SU, protease spesific activity 73.149 U/mg and ratio of MCE to protease 3.29 SU/U. Keywords : incubation time, Enzyme protease, Lactobacillus rhamnosus SKG 34, Milk clotting enzyme.

scholarly journals Pengaruh Perbandingan Puree Wortel (Daucus carota L.) Dan Terigu Terhadap Karakteristik Roti Tawar

2020 ◽  
Vol 9 (2) ◽  
pp. 193
Author(s):  
Norma Yunita ◽  
I Made Sugitha ◽  
I Gusti Ayu Ekawati
Keyword(s):  
Water Content ◽  
Wheat Flour ◽  
Block Design ◽  
Ash Content ◽  
Loaf Volume ◽  
Carotene Content ◽  
Daucus Carota L ◽  
Randomized Block Design ◽  
Carrot Puree ◽  
Orange Color

This research was conducted with the to determine the comparative effect of carrot puree and wheat flour on the characteristic of bread and to know of the exact comparison of carrot puree and wheat flour to obtain the best characteristic of bread. The experimental design used was a Randomized Block Design (RBD) with treatment factor ratio of carrot puree and wheat flour that was 0%: 100%, 10%: 90%, 20%: 80%, 30%: 70%, 40%: 60%, and 50 %: 50%. The treatment was repeated 3 times to obtain 18 experimental units. Data were analyzed with the Anova test and if the treatment had an effect on the variables then proceeded with the Duncan test. The results showed that the comparison of carrot puree and wheat flour affected the water content, ash content, swelling power, total carotene, color, aroma, texture, taste, pore uniformity, and overall acceptance. Comparison of 30% carrot puree: 70% wheat flour produced the best characteristics of bread with the criteria water content of 16.13%, ash content of 1.37%, loaf volume of 123.65% and total carotene content of 13.01 mg/100 g. Orange color, slightly carrot aroma, soft texture, pore uniformity (uniform) liked and overall acceptance of bread very liked. Keywoards : bread, wheat flour, carrot puree.

scholarly journals Karakteristik Fisiko-Kimia dan Sensori Nugget Rumput Laut dengan Penambahan Tepung Ikan Motan (Thynnichthys thynnoides)

2018 ◽  
Vol 6 (2) ◽  
pp. 163-173
Author(s):  
Ricky Setyo Aditomo ◽  
Rodiana Nopianti ◽  
Indah Widiastuti
Keyword(s):  
Protein Content ◽  
Water Content ◽  
Sensory Analysis ◽  
Research Method ◽  
Fish Meal ◽  
Block Design ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Physical Analysis ◽  
Randomized Block Design

The purpose of the research was to know the physicochemical and sensory characteristic of seaweed nugget with an addition of carp fish meal. The research was conducted from March 2016 until February 2017. The research method used a randomized block design (RBD). The treatments were 0%, 10%, 20%, 30% and 40% of fish meal. The parameters of this research were chemical analysis such as water content, ash content, protein content, carbohydrate content, and calcium; physical analysis was hardness, color and sensory analysis (color, taste, and texture). The results showed that treatments gave significant effect on water content, ash content, protein content, lipid content, carbohydrate content, calcium, lightness, chroma, hardness, and taste, but there was no effect on hue, color and texture. The water content was 47.01%-48.19%, ash was 1.29%-4.36%, protein was 2.26%-7.10%, lipid was 11.83%-14.79%, carbohydrate was 26.75%-36.42%, calcium was 15.11 mg/100g-48.46 mg/100g. The lightness of this seaweed nugget were 33.70%-63.43%, chroma 25.00%-35.10%, texture 26.97 gf – 50.80 gf. Taste sensory analysis of seaweed nugget were 2.72 (unlike) – 3.84 (like). The best treatment found in this research was A1.

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